海洋內生真菌 Chondrostereum sp. NTOU4196 之活性物質分析

Abstract

本研究利用抑制一氧化氮生成的活性分析平台來篩選真菌培養株，藉以篩選出具有抗發炎活性效果的真菌株，發現某些真菌株的醱酵培養液的乙酸乙酯萃取物，對於RAW264.7細胞的一氧化氮產生具有顯著的抑制作用，而後選定 Chondrostereum sp. NTOU4196 進行其成分研究。接著以PDY (peptone dextrose yeast) 為培養基擴大培養後，針對醱酵液所含代謝產物進行分析、分離與純化，計獲得 17 個化合物純質，分別命名為： chondroterpene A (1)、chondroterpene B (2)、chondroterpene C (3)、chondroterpene D (4)、chondroterpene E (5)、chondroterpene F (6)、chondroterpene G (7)、chondroterpene H (8)、(+)-hirsutanol A (9)、hirsutanol C (10)、hirsutanol E (11)、ent-gloeosteretriol (12)、chondrosterin B (13)、arthrosporone (14)、chondrosterin A (15)、(+)-antrodol C (16) 及 trans 2,3 epoxydeca-4,6,8 triyn-1-ol (17)，其中倍半萜類化合物 1 9與16 為新化合物，其結構經由光譜資料加以確認。在生物活性測試上，化合物 9 對於BV 2 細胞之一氧化氮生成顯示有強抑制效果，其半抑制濃度 (IC50) 為 5.16 ± 1.15 μM。

The ethyl acetate extracts of the fermented broths of Chondrostereum sp. NTOU4196 were found to exhibit significant growth inhibitory activity against NO production in our preliminary screening. Therefore, separation of the active components from broth were carried out which resulted in the isolation of seventeen compounds. Their structures were elucidated to be chondroterpene A (1)、chondroterpene B (2)、chondroterpene C (3)、chondroterpene D (4)、chondroterpene E (5)、chondroterpene F (6)、chondroterpene G (7)、chondroterpene H (8)、(+)-hirsutanol A (9)、hirsutanol C (10)、hirsutanol E (11)、ent-gloeosteretriol (12)、chondrosterin B (13)、arthrosporone (14)、chondrosterin A (15)、(+)-antrodol C (16) and trans-2,3-epoxydeca-4,6,8-triyn-1-ol (17). Their strucrure were elucidated based on spectral analysis. Of these compounds identified, sesquiterpenes 1 9 and 16 were novel chemical entities. Compound 9 exhibited potent inhibitory activity on NO production with a IC50 value of 5.16 ± 1.15 µM.