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  1. NTU Theses and Dissertations Repository
  2. 生物資源暨農學院
  3. 生物機電工程學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78115
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dc.contributor.advisor郭彥甫(Yan-Fu Kuo)
dc.contributor.authorChia-Hao Liangen
dc.contributor.author梁家豪zh_TW
dc.date.accessioned2021-07-11T14:42:42Z-
dc.date.available2021-11-02
dc.date.copyright2016-11-02
dc.date.issued2016
dc.date.submitted2016-08-16
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78115-
dc.description.abstract花瓣的形狀能夠決定花朵的外觀,因此花瓣的形狀在花卉園藝的領域裡一直是很重視的目標。不同發育情形下的花瓣能夠產生廣泛多變的花瓣形狀,因此研究花瓣的形狀變異也成為了探索花瓣發育不可或缺的研究。此研究的研究材料為第二子代的大岩桐花朵,第一子代花朵為透過將輻射對稱以及兩側對稱的親本雜交培育而成,而第二子代則是將第一子代進行自交而成。第二子代花朵的CYCLOIDEA基因組合型被觀察出與花朵形狀有非常大的關係,並且發育出具有相當大程度型態變異的花朵。為了能夠精確地描述花瓣形狀,此研究以微斷層掃描機掃描花朵取得三維花朵影像,並且透過三維型態學方法分析花瓣主要的形狀變異,透過觀察主要形狀變異定義出花瓣特徵,接著透過相關性分析以及階層式分群分析了解性狀協調,透過了解花瓣性狀的協調能夠了解花瓣發育上的趨勢,另外透過基因性狀關聯性的分析,我們也探討了在CYCLOIDEA基因組合型與花瓣特徵的關係,此外在輻射對稱的大岩桐花朵裡,觀察出背側花瓣有腹側化的現象,因此最後透過郝斯多夫距離量測形狀相似性以了解輻射對稱花朵的腹側化現象。zh_TW
dc.description.abstractStudy of petal shape variation is necessary for exploring petal morphology. Petals are complex in structures and vary tremendously. To describe petal shape accurately, three-dimensional (3D) methods were applied to this study. This study aimed to acquire 3D images using microcomputed tomography (μCT). 3D geometric morphometrics (GM) methods were used to evaluate major petal shape variations. The petal traits were physically identified according to the principal shape variations. The second generation (F2) populations of Sinningia speciosa (S. speciosa) resulting from intercross between a zygomorphic variety and an actinomorphic cultivar were applied to this study. The petal shapes of F2 population in S. speciosa were found largely associated with genotypic combinations of CYCLOIDEA (SsCYC) genotypes. The genotype-phenotype association was investigate to realize whether SsCYC genotypes played a key role in controlling the petal traits. The actinomorphic plants in S. speciosa developed flowers in which dorsal petals resembled the ventral petals. Venrtralization of the dorsal petals on actinomorphic plants was then examined morphologically by measuring the degree of shape similarity using Hausdorff distance. The 3D-GM methods approximately identified the petal shapes variations and defined the petal traits. Numerous petal traits, including petal width and left-right asymmetry of the dorsal petals, recurvation and petal size of the lateral petals, recurvation and lobe size of the ventral petals were identified not associated with SsCYC genotypes. The shape similarity analysis indicated that actinomorphic plants have ventralized phenotypes.en
dc.description.provenanceMade available in DSpace on 2021-07-11T14:42:42Z (GMT). No. of bitstreams: 1
ntu-105-R03631028-1.pdf: 1503374 bytes, checksum: e038ccc82d8027688945ea7ed475ae82 (MD5)
Previous issue date: 2016
en
dc.description.tableofcontentsACKNOWLEDGEMENTS i
摘要 ii
ABSTRACT iii
TABLE OF CONTENTS iv
LIST OF TABLES vi
LIST OF FIGURES vi
CHATPER 1. INTRODUCTION 1
1.1 Evaluation of flower shape variation using geometric morphometrics 1
1.2 Ventralization 1
1.3 Objectives 2
1.4 Organization 3
CHATPER 2. LITERATURE REVIEW 4
2.1 Evaluation of flower shape variation using geometric morphometrics 4
2.2 Genotype-phenotype association of the floral development 5
2.3 Ventralization 5
CHATPER 3. MATERIAL AND METHODS 6
3.1 Plant materials and SsCYC genotyping 7
3.2 Image acquisition and quality improvement 8
3.3 Landmark selection 9
3.4 Identification and visualization of the major shape variations of the petals 10
3.5 Major morphological traits of the petals 11
3.6 Association between the SsCYC genotypes and defined traits 12
3.7 Ventralization of the dorsal petals on the actinomorphic plants 12
CHATPER 4. RESULTS 14
4.1 Major shape variations of the petals 15
4.2 Morphological traits of the petals 19
4.3 Association between the SsCYC genotypes and defined traits 23
4.4 Ventralization of the dorsal petals on the actinomorphic plants 24
CHATPER 5. DISCUSSIONS 26
5.1 Phenotyping for the petal traits 26
5.2 Association between the SsCYC genotypes and defined traits 26
5.3 Peloric Sinningia, ventralization of the dorsal petals 27
CHATPER 6. CONCLUSIONS 29
REFERENCES 30
dc.language.isoen
dc.subject腹側化zh_TW
dc.subjectCYCLOIDEAzh_TW
dc.subject三維分析zh_TW
dc.subject花瓣形狀變異zh_TW
dc.subject基因性狀關連性zh_TW
dc.subject背腹不對稱zh_TW
dc.subject大岩桐zh_TW
dc.subjectSinningia speciosaen
dc.subjectventralizationen
dc.subjectgenotype-phenotype associationen
dc.subjectpetal shape variationen
dc.subjectdorsoventral asymmetryen
dc.subjectthree-dimensional analysisen
dc.subjectCYCLOIDEAen
dc.title透過微斷層掃描機照射分析大岩桐花瓣形狀變異:輻射對稱和兩側對稱花朵的雜交系zh_TW
dc.titleStudying petal shape variations of Sinningia speciosa using micro-CT: a crossed line of actinomorphic and zygomorphic flowersen
dc.typeThesis
dc.date.schoolyear104-2
dc.description.degree碩士
dc.contributor.oralexamcommittee王俊能(Chun-Neng Wang),林彥蓉(Yann-rong Lin)
dc.subject.keyword大岩桐,CYCLOIDEA,三維分析,背腹不對稱,花瓣形狀變異,基因性狀關連性,腹側化,zh_TW
dc.subject.keywordSinningia speciosa,CYCLOIDEA,three-dimensional analysis,dorsoventral asymmetry,petal shape variation,genotype-phenotype association,ventralization,en
dc.relation.page36
dc.identifier.doi10.6342/NTU201602629
dc.rights.note有償授權
dc.date.accepted2016-08-17
dc.contributor.author-college生物資源暨農學院zh_TW
dc.contributor.author-dept生物產業機電工程學研究所zh_TW
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