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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 杜宜殷(Yi-Yin Do) | |
dc.contributor.author | Chia-Chun Chang | en |
dc.contributor.author | 張佳君 | zh_TW |
dc.date.accessioned | 2021-07-11T14:40:44Z | - |
dc.date.available | 2022-02-21 | |
dc.date.copyright | 2017-02-21 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-10-13 | |
dc.identifier.citation | 王小榮、劉選明、劉斌. 2003. 不同激素組合對苦瓜離體快速繁殖的調控.湖南師範大學自科學學報26:76-78.
江筱慧. 2008.苦瓜質脂結合蛋白cDNA 之選殖與分析. 國立臺灣大學生物資源暨農學院園藝暨景觀學系碩士論文. 汪俏梅、曾廣文. 1997. 苦瓜性別分化的型態與組織化學研究. 浙江農業大學學報 23:149-153 汪俏梅、曾廣文. 2002. Studies on expression of sex differentiation program in Momordica charantica L. 浙江農業大學學報 28:243-248 林生華、杜宜殷. 2008. 轉殖順義ACC合成酶基因延緩苦瓜果實後熟之研究. 台灣園藝 54:115-125 張有明. 1997. 苦瓜組織培養再生、農桿菌媒介法及花粉電穿孔法之基因轉殖研究. 國立臺灣大學園藝學系博士論文. 陳君琳. 2012. 苦瓜質脂結合蛋白基因McPAP1之功能分析. 國立臺灣大學生物資源暨農學院園藝暨景觀學系碩士論文. 陳柏亨. 2014. 胡瓜及苦瓜轉殖表達外源質脂結合蛋白基因McPAP1之研究. 國立臺灣大學生物資源暨農學院園藝暨景觀學系碩士論文. 游沐慈. 2010. 苦瓜質脂結合蛋白McPAP1之功能分析及蛋白質定位. 國立臺灣大學生物資源暨農學院園藝暨景觀學系碩士論文. 賈儒珍. 2005. 苦瓜性別表現相關蛋白質之研究. 國立臺灣大學園藝學系碩士論文. Agarwal, M., and Raka Kamal. 2004. In vitro clonal propagation of Momordica charantia L. Indian J. of Biotechnol. 3:426-430. An, D. B., K. Eggermont, P. Druart, M. D. Vil, I. Goderis, J. Vanderleyden, and W. F. Broekaert. 1994. Agrobacterium-mediated transformation of apple (Malus × domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. Plant Cell Rep. 12:587-593. Ayako, N.-Y., M. Endo, K. Osakabe, H. Saika, and S. Toki. 2014. Precise marker excision system using an animal-derived piggyBac transposon in plants. Plant J. 77:454-463. Aye, M. M., Y. Sung, and W. N. Chang. 2004. Techniques improved ‘Ching Pi’ bitter gourd (Momordica charantia L.) seed germination. Hort. NCHU 29:27-42. Bai, S. L., Y. B. Peng, J. X. Cui, H. T. Gu, L. Y. Xi, Y. Q. Li, Z. H. Xu, and S. N. Bai. 2004. Developmental analyses reveal early arrest of the sporebearing parts of reproductive organs in unisexual flowers of cucumber (Cucumis sativus L.). Planta 220:230-240. Baskaran, P., V. Soós, E. Balázs, and J. Van Staden. 2016. Shoot apical meristem injection: A novel and efficient method to obtain transformed cucumber plants. S. Afr. J. Bot. 103:210-215. Bisaria A.K. 1974. The effect of foliar sprays of alpha naphthalene acetic acid on sex expression in Momordica charantia L. Sci. Cult. 40:70-80. Boualem, A., M. Fergany, R. Fernandez, C. Troadec, A. Martin, H. Morin, M. A. Sari, F. Collin, J. M. Flower, M. Pitrat, M. Pitrat, M. D. Purugganan, C. Dogimont, and A. Bendahmane. 2008. A conserved mutation in an ethylene biosynthesis enzyme leads to andromonoecy in melons. Science 321:836-838. Cervera, M., J. A. Pina, J. Juárez, L. Navarro, L. Peña. 1988. Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration. Plant Cell Rep. 18:271-278. Chee, P.P. 1990. Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants. Plant Cell Rep. 9:245-248. Chee, P. P. and J. L. Slightom. 1991. Transfer and expression of Cucumber Mosaic Virus coat protein gene in the genome of Cucumis sativus. J. Amer. Soc. Hort. Sci. 116:1098-1102. Deruère, J., S. Römer, A. d’Harlingue, R.A. Backhaus, M. Kuntz, and B. Camara. 1994. Fibril assembly and carotenoid overaccumulation in chromoplasts: a model for superamolecular lipoprotein structures. Plant Cell 6:119-133. Galun, E. 1961. Study of the inheritance of sex expression in the cucumber: the interaction of major genes with modifying genetic and non-genetic factors. Genetica 32:134-163. Ganapathi, A. and R. Perl-Treves. 2000. Agrobacterium-mediated transformation in Cucumis sativus via direct organogenesis. Acta Hort. 510:405-407. Gaspar, Thomas., C. Kevers, C. Penel, H. Grepppin, D. M. Reid, and T. A. Thorpe. 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev. Biol. Plant 32:272-289. Goffinet, M. 1990. Comparative ontology of male and female flowers of Cucumis sativus. In: D.M. Bates, R.W. Robinson, C. Jefferey (eds) Biology and utilization of the Cucurbitaceae. Cornell Univ. Press., Ithaca, p 288-304. He, Zhengquan., Z. Z. Duan, W. Liang, F. Chen, W. Yao, H. Liang, C. Yue, Z. Sun, F. Chen, and J. Dai. 2006. Mannose selection system used for cucumber transformation. Genet. Transform. Hybridization 25:953-958. Javier, P.-R., F. Rafia, C. Houlné, C. Cheniclet, j.-P. Carde, M.-L. Schantz, and R. Schantz. 1997. A ubiquitous plant housekeeping gene, PAP, encodes a major protein component of bell pepper chromoplasts. Plant Physiol. 115:1185-1194. Kahana, A., L. Silberstein, N. Kessler, R. S. Goldstein, and R. Perl-Treves. 1999. Expression of ACC oxidase genes differs among sex genotypes and sex phases in cucumber. Plant Mol. Biol. 41:517-528. Kamachi, S., H. Mizusawa, S. Matsuura, and S. Sakai. 2000. Expression of two 1-aminocyclopropane-1-carboxylate synthase genes, CS-ACS1 and CS-ACS2, correlated with sex phenotypes in cucumber plants (Cucumis sativus L.) Plant Biotechnol. 17:69-74. Kim, H. A., S. R. Min, D. W. Choi, P. S. Choi, and S. G. Hong. 2010. Development of transgenic cucumber expressing TPSP gene and morphological alterations. J. Plant Biotechnol. 37:72-76. Kose, E., and N. K. Koç. 2003. Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) and plant regeneration. Biotechnol. Equip. 17:56-62. Kubicki, B. 1969. Investigation of sex determination in cucumber (Cucumis sativus L.). Genetica Polonica 10:69-143. Leitner-dagan, Y., M. Ovadis, E. Shklarman, Y. Elad, D. R. David, and A. Vainstein. 2006. Expression and functional analyses of the plastid lipid-associated protein CHRC suggest its role in chromoplastogenesis and stress. Plant Physiol. 142:233-244. Li, Z., S. Huang, S. Liu, J. Pan, Z. Zhang, Q. Tao, Q. Shi, Z. Jai, W. Zhang, H. Chen, L. Si, L. Zhu, and R. Cai. 2009. Molecular isolation of the M gene suggests that a conserved-residue conversion induces the formation of bisexual flowers in cucumber plants. Genetics 182:1381-1385. Li, Z., S. Wang, Q. Tao, J. Pan, L. Si, Z. Gong, and R. Cai. 2012 A putative positive feedback regulation mechanism in CsACS2 expression suggests a modified model for sex determination in cucumber (Cucumis sativus L.). J. Expt. Bot. 63:4475-4484. Margie, M. P., H. Shou, Z. Guo, Z. Zhang, A. K. Banerjee, and K. Wang. 2004. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica 136:167-179. Martin, A., C. Troadec, A. Boualem, M. Rajab, R. Fernandez, H. Morin, M. Pitrat, C. Dogimont, and A. Bendahmane. 2009. A transposon-induced epigenetic change leads to sex determination in melon. Nature 461:1135-1138. McWilliam, A. A., S. M. Smith, and H. S. Street. 1974. The origin and development of embryoids in suspension cultures of carrot (Daucus carota). Ann. Bot. 38:243-250. Mibus, H. and T. Tatlioglu. 2004. Molecular characterization and isolation of the F/f gene for femaleness in cucumber (Cucumis sativus L.). Theor. Appl. Genet. 109:1669-1676. Poole, C.F. and P. C. Grimball. 1939. Inheritance of new sex forms in Cucumis melo L. J. Hered. 30:21-25. Qi, X. H., X. W. Xu, X. J. Lin, W. J. Zhang, and X. H. Chen. 2012. Identification of differentially expressed genes in cucumber (Cucumis sativus L.) root under waterogging stress by digital gene expression profile. Genomics 99:160-168. Rajagopalan P. A. and R. Perl-Treves. 2005. Improved cucumber transformation by a modified explant dissection and selection protocol. HortScience 40:431-435. Rey, P., B. Gillet, S. Römer, F. Eymery, J. Massimino, G. Peltier, and M. Kuntz. 2000. Over-expression of a pepper plastid lipid-associated protein in tobacco leads to changes in plastid ultrastructure and plant development upon stress. Plant J. 21:483-494. Robinson, R. W., H. M. Munger, T. W. Whitaker, and G. W. Bohn. 1976. Genes of the Cucurbitaceae. HortScience 11:554-568. Selvaraj N., S. Kasthurirengan, A. Vasudevan, M. Manickavasagam, C. W. Choi, and A. Ganapathi. 2010. Evaluation of green fluorescent protein as a reporter gene and phosphinothricin as the selective agent for achieving a higher recovery of transformants in cucumber (Cucumis sativus L. cv. Poinsett76) via Agrobacterium tumefaciens. In Vitro Cell Dev. Biol. 46:329-337. Sikdar. B., M. Shafiullah, A. R. Chowdhury, N. Sharmin, S. Nahar, and O. I. Joarder. 2005. Agrobacterium-mediated GUS Expression in Bitter Gourd (Momordica charantia L.) Biotechnol. 4:149-152. Simkin, A.J., J. Gaffe, J.P. Alcaraz, J.P. Carde, P.M. Bramley, P.D. Fraser, and M. Kuntz. 2007. Fibrillin influence on plastid ultrastructure and pigment content in tomato fruit. Phytochemistry 68:1545-1556. Singh, D.K., S.N. Maximova, P.J. Jensen, B.L. Lehman, H.K. Ngugi, and T.W. McNellis. 2010. FIBRILLIN4 is required for plastoglobule development and stress resistance in apple and Arabidopsis. Plant Physiol. 154:1281-1293. Sureshkumar, P., N. Selvaraj, A. Ganapathi, S. Kasthuriengan, A. Vasudevan, and V. R. Anbazhagan. 2005. Assessment of factors influencing Agrobacterium mediated transformation in cucumber (Cucumis Sativus L.). J. Plant Biotechnol. 74:225-231. Switzenberg, J. A., H. A. Little, S. A. Hammer, and R. Grumet. 2014. Floral promordia-targeted ACS (1-aminocyclopropane-1-carboxylate synthase) expression in transgenic Cucumis melo implicated fine tuning of ethylene production mediating unisexual flower development. Planta 240:797-808. Thiruvengadam, M., K. T. Rekha, C. H. Yang, N. Jayabalan, and I. M. Chung. 2010. High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.). Plant Biotechnol. Rep. 4:321-328. Thiruvengadam, M., N. Praveen, and I. M. Chung. 2012. In vitro regeration from intermodal explants of bitter melon (Momordica charantia L.) via indirect organogenesis. Afr. J. of Biotechnol. 11:8218-8224. Thiruvengadam, M., N. Praveen, and I. M. Chung. 2012. An efficient Agrobacterium tumefaciens – mediated genetic transformation of bitter melon (Momordica charantia L.). Astralian J. of crop Sci. 6:1094-1100. Thiruvengadam, M., S. V. Mohamed, C. H. Yang, N. Jayablan. 2006. Development of an embryogenic suspension culture of bitter melon (Momordica charantia L.). Scientia Hort. 109:123-129. Thomas, T. D. 2008. The effect of in vivo and in vitro applications of ethrel and GA3 on sex expression in bitter melon (Momordica charantia L.). Euphytica 164:317-323. Trebitsh, T., J. E. Staub, and S. D. O’Neill. 1997. Identification of a 1-aminocyclopropane-1 -carboxylic acid synthase gene linked to the Female (F) locus that enhances female sex expression in cucumber. Plant Physiol. 113:987-995. Vasudevan A, N. Selvaraj, A. Ganapathi, and C. W. Choi. 2007. Agrobacterium-mediated genetic transformation in cucumber (Cucumis sativus L.). Am. J. Biochem. Biotechnol. 3:24-32. Vishnevetsky, M., M. Ovadis, H. Itzhaki, and A. Vainstein. 1997. CHRC, Encoding a chromoplast-specific carotenoid-associated protein, is an early gibberellic acid-responsive gene. J. Biol. Chem. 272:24747-24750. Vishnevetsky, M., M. Ovadis, H. Itzhaki, M. Levy, Y. Libal-Weksler, Z. Adam, and A. Vainstein. 1996. Molecular cloning of a carotenoid-associated protein from Cucumis sativus corollas: homologous genes involved in carotenoid sequestration in chromoplast. Plant J. 10:1111-1118. Wang, S. L., S. K. Seong, X. G. YE, C. F. HE, Y. K. Suk, and S. C. Pil. 2015. Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.). J. Integr. Agric. 14:469-482. Wu, T., Z. Qin, X. Zhou, Z. Feng, and Y. Du. 2010. Transcriptome profile analysis of floral sex determination in cucumber. J. Plant Physiol. 167:905-913. Yamasaki, S., N. Fujii, S. Matsuura, H. Mizusawa, and H. Takahashi. 2001. The M locus and ethylene-controlled sex determination in andromonoecious cucumber plants. Plant Cell Physiol. 42:608-619. Yamazaki, S., N. Fujii, and H. Takahashi. 2003. Characterization of ethylene effects on sex determination in cucumber plants. Sex Plant Rep. 16:103-111. Yin, Z., G. Bartoszewski, M. Szwacka, and S. Malepszy. 2005. Cucumber transformation methods - the review. Biotechnologia 1:95-113. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78051 | - |
dc.description.abstract | 為了改良胡瓜及苦瓜基因轉殖系統及再生條件,進行培養基配方及轉殖方法之測試,轉殖質體具有過量表現或默化McPAP1基因之構築,有助於瞭解McPAP1對胡蘆科花性的影響及應用於其他胡瓜及苦瓜基因轉殖分析。由癒傷組織再生之過量表現McPAP1胡瓜轉殖株於瓶內開花,默化McPAP1轉殖胡瓜之再生芽體普遍畸形,限制轉殖植株發育出瓶,McPAP1融合綠色螢光蛋白定位構築轉殖效率低。胡瓜以農桿菌生長點注射法,取得具抗生素抗性之McPAP1基因默化及McPAP1蛋白定位擬轉殖株。將McPAP1融合綠色螢光蛋白定位基因構築置於PiggyBac 轉座子系統之載體中,以農桿菌共培養法轉殖至胡瓜癒傷組織,結果顯示轉殖率較pGKU高約2.5倍。以苦瓜品種‘月華’、‘高月’、‘小月’及‘英翠’之子葉、莖段、葉片所誘導出之癒傷組織形態均相似,分裂旺盛之細胞呈現透明鬆散狀,進行含不同濃度kinetin、TDZ、BA組合之培養基,或以不同濃度2,4-D懸浮培養,皆無法誘導芽體再生。將苦瓜種子無菌播種於添加100 µM乙醯丁香酮 (actosyringone, AS) 之培養基白化幼苗的頂端分生組織 (shoot apical meristem, SAM),以含100 µM AS之 YEB懸浮農桿菌至OD = 0.8的注射液,進行苦瓜生長點農桿菌注射法基因轉殖,有最佳的再生及擬轉殖株抗生素抗性表現。 | zh_TW |
dc.description.abstract | Not only to improve the generation and transformation efficiency in cucumber (Cucumis sativus L.) and bitter gourd (Momordica charantia L.), but also understand the relationship of McPAP1 and flower sexuality in Cucurbiteae. The effects of medium composition and delivery system were investigated using constructs for overexpression, silencing, and protein localization of McPAP1. Callus generated from cotyledon of cucumber and leaf of bitter gourd were transformed via Agrobacterium-mediated method. McPAP1-overexpressed cucumbers bloomed in vitro and McPAP1-silencing cucumbers produced abnormal shoots. Transposon PiggyBac increased 2.4 folds of transformation efficiency of Agrobacterium-mediated method in cucumber. The types of callus induced from cotyledon, stem, leaf disc of 4 bitter gourd varieties ‘Moon Shine’, ‘High moon’, ‘New moon’, and ‘F-2486’ are all whitish-green and friable. Different concentrations and combinations of kinetin, TDZ, and BA in MS medium and suspension culture in MS medium supplemented with different concentrations of 2,4-D were tested but failed to induced shoots from callus. Shoot apical meristems adapted from 5 to 7-day-old etiolated seedings were injected with Agrobacterium broth in YEB supplemented with 100 µM actosyringone and putative transgenic bitter gourds were obtained. | en |
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dc.description.tableofcontents | 審查委員………………………………..……………….………………….i
論文口試委員審定書…………………………………….…………..……ii 誌謝………………………………………………………………………iii 摘要………………………………………………………………………iv Abstract……………………………………………….………………….v 圖目錄…………………………………………………….………………xi 表目錄……………………………………………………………………xii 壹、 前言.................................................................................................1 貳、 前人研究.........................................................................................2 一、 胡瓜轉殖及再生系統..............................................................2 (一) 胡瓜組織培養及再生系統.......................................................2 (二) 胡瓜農桿菌基因轉殖系統........................................................2 二、 苦瓜組織培養.........................................................................3 (一) 苦瓜種子無菌播種....................................................................3 (二) 苦瓜癒傷組織之誘導................................................................3 (三) 苦瓜芽體再生系統....................................................................4 三、 苦瓜基因轉殖.......................................................................5 (一) 花粉電穿孔法.........................................................................5 (二) 農桿菌基因轉殖法...................................................................5 四、 甜瓜與胡瓜的花性分子遺傳.................................................6 (一) 甜瓜花性相關基因...................................................................6 1. 控制甜瓜花性基因座 (locus)…...............................................6 2. A/a 基因座......................................................................6 3. G/g 基因座......................................................................7 4. 甜瓜基因決定花性分化之模式................................................7 (二) 胡瓜花性相關基因....................................................................7 1. 影響胡瓜花性基因座...............................................................7 2. F/f基因座.....................................................................8 3. M/m基因座......................................................................8 4. 胡瓜F及M基因影響花性分化之模式.....................................9 五、 質脂結合蛋白........................................................................9 (一) 從甜椒中看質脂結合蛋白之生理功能..................................10 (二) 胡瓜中質脂結合蛋白之基因調控..........................................10 (三) 苦瓜花性相關基因McPAP1之特性.......................................11 參、 材料與方法..................................................................................13 一、 試驗材料..............................................................................13 (一) 質體材料..................................................................................13 (二) 試驗菌種..................................................................................13 (三) 植物材料.................................................................................13 二、 試驗方法...............................................................................14 (一) 胡瓜基因轉殖及再生系統......................................................14 (二) 不同載體之胡瓜癒傷組織轉殖效率分析..............................14 1. pGnDxKPBgg-McPAPgfp之載體構築...................................14 2. pGnDxKPBgg-siMcPAP之載體構築……………..................14 3. 胡瓜癒傷組織轉殖效率分析………………………………15 (三) 苦瓜組織培養再生系統.................................................15 1. 苦瓜種子發芽處理..................................................................15 2. 不同生長調節劑組合對苦瓜癒傷組織之影響......................15 3. 苦瓜不同部位之癒傷組織誘導..............................................16 4. 不同生長調節劑組合誘導苦瓜培植體癒傷組織..................16 5. 苦瓜‘月華’生長點之芽體誘導及芽體發根試驗...................16 (四) 苦瓜基因轉殖...................................................................17 1. 農桿菌培養..............................................................................17 2. 農桿菌感染液配製…………………………………………17 3. 農桿菌共培養基因轉殖法…………......................................17 4. 農桿菌注射液配製…………………………………………17 5. 頂端分生組織農桿菌注射法..................................................18 (五) 胡瓜轉殖芽體之分子驗證......................................................19 1. GUS活性化學染色分析.........................................................19 2. 植物基因組DNA之抽取……………………………………19 3. 聚合酶連鎖反應......................................................................19 (六) 胡瓜轉殖芽體基因表現分析..................................................20 1. 植物總RNA抽取………........................................................20 2. cDNA合成……………………………………………………20 3. 即時定量聚合酶連鎖反應......................................................20 肆、 結果..............................................................................................22 一、 胡瓜基因轉殖.......................................................................22 (一) 胡瓜癒傷組織農桿菌感染法之轉殖結果..............................22 (二) 胡瓜頂端分生組織農桿菌注射法之轉殖結果......................22 (三) 過量表現及靜默McPAP1胡瓜芽體之基因表現量分析.......22 二、 苦瓜‘高月’癒傷組織誘導芽體試驗.....................................23 (一) 不同濃度TDZ及BAP對苦瓜‘高月’癒傷組織分化之 影響.......................................................................................23 (二) 不同濃度2,4-D液態培養對苦瓜‘高月’癒傷組織分化之 影響………………………………………………………23 三、 誘導之苦瓜癒傷組織形態分類……………………………24 四、 苦瓜生長點農桿菌注射系統之建立………………………24 (一) 苦瓜生長點再生芽體發根試驗……………………………24 (二) 苦瓜生長點農桿菌注射…………………………………25 五、 不同載體之轉殖效率 .........................................................26 伍、 討論............................................................27 一、 質脂結合蛋白對植物再生的影響...................................27 二、 調控質脂結合蛋白基因表現對胡瓜花性相關基因表現 的影響…………………………………………………………27 三、 影響胡瓜基因轉殖效率的因子...............................................29 四、 苦瓜癒傷組織之形態再生芽體分化能力.................................30 五、 影響苦瓜生長點農桿菌注射之轉殖效率的因子....................30 陸、 結語.............................................................................................32 引用文獻...................................................................................................60 附錄...........................................................................................................69 | |
dc.language.iso | zh-TW | |
dc.title | 胡瓜及苦瓜基因轉殖系統之研究 | zh_TW |
dc.title | Studies on Genetic Transformation in Cucumber (Cucumis sativus L.) and Bitter Gourd (Momordica charantia L.) | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 黃鵬林(Pung-Ling Huang) | |
dc.contributor.oralexamcommittee | 廖芳心(Fang-Shin Liao),張有明(Yu-Ming Chang),何錦玟(Chin-Wen Ho) | |
dc.subject.keyword | 胡瓜,苦瓜,基因轉殖,芽體再生, | zh_TW |
dc.subject.keyword | Cucumber,bitter gourd,genetic transformation,shoots regeneration, | en |
dc.relation.page | 69 | |
dc.identifier.doi | 10.6342/NTU201603662 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-10-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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