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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王愛玉(Ai-Yu Wang) | |
| dc.contributor.author | Yen-Han Lin | en |
| dc.contributor.author | 林延翰 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:40:43Z | - |
| dc.date.available | 2022-02-21 | |
| dc.date.copyright | 2017-02-21 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-10-13 | |
| dc.identifier.citation | 林維治 (1996) 林維治先生竹類論文集，臺灣省林業試驗所
張皓鈞 (2012) 綠竹中 BoMSP41 基因之分子選殖與檢定，碩士論文，國立臺灣大學生命科學院生化科技學系 楊芳潔 (2013) 綠竹 BoMSP41 之細胞內定位與生化性質探討，碩士論文，國立臺灣大學生命科學院生化科技學系 李佩儒 (2014) 重組綠竹 Monocot-Specific Protein-41 之純化與性質檢定，碩士論文，國立臺灣大學生命科學院生化科技學系 王人雙 (2015) 綠竹 BohLOL1 基因之選殖與分析，碩士論文，國立臺灣大學生命科學院生化科技學系 黃塏荔 (2015) 綠竹 BoMSP41 轉殖水稻之建立，碩士論文，國立臺灣大學生命科學院生化科技學系Auerbach D, Thaminy S, Hottiger MO, Stagljar I (2002) The post-genomic era of interactive proteomics: facts and perspectives. Proteomics 2: 611-623 Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (2003) Current Protocols in Molecular Biology. John Wiley & Sons, Inc. Bah A, Vernon RM, Siddiqui Z, Krzeminski M, Muhandiram R, Zhao C, Sonenberg N, Kay LE, Forman-Kay JD (2015) Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch. Nature 519: 106-109 Bannai H, Tamada Y, Maruyama O, Nakai K, Miyano S (2002) Extensive feature detection of N-terminal protein sorting signals. 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Bioinformatics 24: 1401-1402 Williams RM, Obradovi Z, Mathura V, Braun W, Garner EC, Young J, Takayama S, Brown CJ, Dunker AK (2001) The protein non-folding problem: amino acid determinants of intrinsic order and disorder. Pac Symp Biocomput 2001: 89-100 Wright PE and Dyson HJ (2009) Linking folding and binding. Curr Opin Struct Biol 19: 31-38 Wu FH, Shen SC, Lee LY, Lee SH, Chan MT, Lin CS (2009) Tape-Arabidopsis sandwich—a simpler Arabidopsis protoplast isolation method. Plant Methods 5: 16 Yeh SH, Lee BH, Liao SC, Tsai MH, Tseng YH, Chang HC, Yang CC, Jan HC, Chiu YC, Wang AY (2013) Identification of genes differentially expressed during the growth of Bambusa oldhamii. Plant Physiol Biochem 63: 217-226 Zhang Y, Su J, Duan S, Ao Y, Dai J, Liu J, Wang P, Li Y, Liu B, Feng D, Wang J, Wang H.A (2011) highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes. Plant Methods 7: 30 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78050 | - |
| dc.description.abstract | 綠竹筍出土後生長快速的現象早有記錄，但至今綠竹生長相關的調控機制尚未被建立。BoMSP41 (Bambusa oldhamii monocot specific protein-41) 為一個於綠竹筍快速生長的節間中表現量大幅提升的基因，但對其功能尚不清楚。而 BoMSP41 蛋白質被預期是無穩定構形蛋白質（Intrinsically disorder protein, IDP），可能會透過與不同生物分子結合後，形成穩定的構形參與不同的生理反應。透過酵母菌雙雜合系統（Yeast two hybrid, Y2H）於綠竹筍 cDNA library 進行篩選可能與 BoMSP41 有交互作用之蛋白質，然而，篩選到的 185 株候選菌株於後續酵母菌雜交驗證中，皆未有對 BoMSP41 有專一性交互作用的候選目標。另一方面，於綠竹未展開葉跟阿拉伯芥之原生質體中，利用短暫性表現 GFP-BoMSP41 融合蛋白質去分析 BoMSP41 的細胞內定位，可於阿拉伯芥的原生質體中觀察到 BoMSP41 位於細胞膜；而於綠竹原生質體中則是觀察到 BoMSP41 存在於細胞質、細胞膜與葉綠體，因觀察到 BoMSP41 位於細胞膜上，推測 BoMSP41 可能會與膜蛋白質產生交互作用或是 BoMSP41 可能屬於單側嵌膜蛋白質 (integral monotopic protein)。此外，BoMSP41 會與染色質產生交互作用，雖目前無法確認是否為直接與 DNA 有交互作用。BoMSP41 出現於多樣性的胞器之中，推測 BoMSP41 可能涉及多種不同的生理反應之中。 | zh_TW |
| dc.description.abstract | The fast-growing feature of bamboo shoots has been recorded, but the related regulatory mechanism has not been elucidated. BoMSP41 (Bambusa oldhamii monocot specific protein- 41) is an unknown function gene that is highly up-regulated in rapidly elongating bamboo shoots. The protein encoded by this gene was suggested to be an intrinsically disorder protein (IDP), which might interact with other biomolecules to perform its function. To identify BoMSP41-interacting proteins, yeast two-hybrid screening was performed with a bamboo shoots cDNA library. Although 185 candidates were obtained, none of them specifically interacted with BoMSP41 after confirmation. Transient expression of GFP-BoMSP41 fusion proteins in protoplasts isolated from Arabidopsis leaves and bamboo unexpanded leaves showed that BoMSP41 was localized in the plasma membrane of Arabidopsis protoplasts, and in the cytoplasm, plasma membrane and chloroplasts of bamboo protoplasts. Localization of BoMSP41 in plasma membrane suggested that the protein may interact with membrane proteins or it is a kind of integral monotopic protein. In addition, BoMSP41 was found to interact with chromatin; however, whether it directly interact with DNA or not remains unknown. Presence of BoMSP41 in various cellular compartments suggested that this protein participate in multiple pathways. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:40:43Z (GMT). No. of bitstreams: 1 ntu-105-R03B22026-1.pdf: 10325348 bytes, checksum: b4def21e031e164c449fa68cf7a99d20 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 目錄 ……………………………………………………………………………………… II
縮寫表…………………………………………………………………………………… IV 摘要………………………………………………………………………………………… VI Abstract …………………………………………………………………………… VII 第一章 緒論 ………………………………………………………………………… 1 1.1 綠竹功能未知基因-BoMSP41 ……………………………… 1 1.2 BoMSP41 之蛋白質特性 ………………………………………… 1 1.3 無穩定構形蛋白質（intrinsically disorder protein, IDP） ……… 2 1.4 BoMSP41 之細胞內定位 ………………………………………… 3 1.5 本篇論文的研究主題與目的 …………………………………… 4 第二章 實驗材料與儀器 ……………………………………………………… 5 2.1 實驗材料 …………………………………………………………… 5 2.2 實驗藥品與儀器 …………………………………………………… 8 第三章 實驗方法 ……………………………………………………………… 10 3.1 Chromatin enrichment for proteomics (ChEP) …………………… 10 3.2 酵母菌雙雜合系統 (Yeast Two-hybrid System, Y2H) …………… 12 3.3 短暫表現重組螢光蛋白質 ………………………………………… 17 第四章 結果 …………………………………………………………………… 20 4.1 Chromatin enrichment for proteomics (ChEP) …………………… 20 4.2 酵母菌雙雜合系統 (Yeast Two-hybrid System, Y2H) …………… 20 4.3 短暫表現重組螢光蛋白質 ………………………………………… 23 第五章 討論 …………………………………………………………………… 25 5.1 以 Y2H 未篩選得與 BoMSP41 有交互作用之蛋白質的可能原因 ………………………………………………………………………… 25 5.2 短暫表現螢光重組蛋白質觀察 BoMSP41 的細胞內定位 ……… 27 第六章 未來展望 ……………………………………………………………… 30 6.1 BoMSP41 與蛋白質交互作用 …………………………………… 30 6.2 BoMSP41 與 DNA 交互作用 ……………………………………… 30 6.3 綠竹筍之原生質體的製備 ………………………………………… 30 6.4 建構 BoMSP41-GFP 觀察細胞內定位 …………………………… 31 參考文獻 ……………………………………………………………………… 32 圖與表 …………………………………………………………………………… 36 附錄圖與表 ……………………………………………………………………… 54 | |
| dc.language.iso | zh-TW | |
| dc.subject | 膜蛋白質 | zh_TW |
| dc.subject | 綠竹 | zh_TW |
| dc.subject | 無穩定構形蛋白質 | zh_TW |
| dc.subject | BoMSP41 | zh_TW |
| dc.subject | Membrane protein | en |
| dc.subject | Bamboo | en |
| dc.subject | BoMSP41 | en |
| dc.subject | Intrinsically disorder protein | en |
| dc.title | 綠竹 BoMSP41 與其他生物分子交互作用之探討 | zh_TW |
| dc.title | Studies on the interactions between BoMSP41 and other biomolecules in Bambusa oldhamii | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林崇熙(Chung-Hsi Lin),楊健志(Chien-Chih Yang),洪傳揚(Chwan-Yang Hong),葉汀峰(Ting-Feng Yeh) | |
| dc.subject.keyword | 綠竹,BoMSP41,無穩定構形蛋白質,膜蛋白質, | zh_TW |
| dc.subject.keyword | Bamboo,BoMSP41,Intrinsically disorder protein,Membrane protein, | en |
| dc.relation.page | 57 | |
| dc.identifier.doi | 10.6342/NTU201603664 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-10-13 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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