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Quantifying In-Vivo Tissue Optical Parameters of Precancerous Cervical Lesions using a Portable Reflectance Spectroscopy System
Diffuse Reflectance Spectroscopy,Monte Carlo method,Mucosal tissue,scattering coefficient,absorption coefficient,precancerous lesions,
|Publication Year :||2017|
本實驗室利用蒙地卡羅演算法模擬光子在不同散射及吸收特性組織內之行進情形，藉此得到蒙地卡羅順向模擬光譜，再透過順向模擬光譜進行組織漫反射光譜校正，目前採用光源光纖與六根不同距離(Source to Detection Separation, SDS)光纖之分析模式，並以兩種不同直徑的擬組織散射小球(Polystyrene)及血紅素調配而成之仿體進行校正，最後利用光譜擬合工具擬合校正後之子宮頸漫反射光譜，萃取出組織特性之相關光學參數，如:散射係數、吸收係數、血氧飽和、血紅素濃度、膠原蛋白濃度及上皮層厚度等。
In clinical, cervical tissue lesions or not need to rely on professional training physician through colposcopy to enlarge the view of suspicious sites. In the case of minor, the surface tissue is sampled in a non-invasive manner such as pap test. If more severe, the tissue is sampled in a invasive manner, and the sample is diagnosed by the pathologist. Due to the need to determine by the pathologist, the results are more subjective and the process is quite time-consuming. This study hope to extract the tissue optical parameters of the suspected sites through real-time non-invasive optical diagnosis. By analyzing the differences in parameters inform physician organizational changes and provide real-time medical information. In addition to assisting physician diagnosis, also improve the hospital diagnosis efficiency.
In this laboratory, the Monte Carlo algorithm is used to simulate the traveling conditions of photons in different scattering and absorption properties. The Monte Carlo simulation spectra are obtained and used to correct the diffuse reflectance spectra of the tissue. The analysis mode of source fiber and six different Source to Detection Separation (SDS) fiber is used to calibrate with phantoms consist of different concentration of microspheres (Polystrene) and hemoglobin. The reverse spectral fitting tools fit the corrected diffuse reflectance spectra of the cervix and extract the relevant optical parameters of the tissue properties such as scattering coefficient, absorption coefficient, oxygen saturation, hemoglobin concentration, collagen concentration and epithelial thickness.
This study is based on the previous laboratory Zhuang Minjie set up by the portable reflectance spectroscopy system combined with multiple multi-mode fiber to provide light source fiber with different distance detection fiber measurement of cervical mucosa. The optical parameters of the tissue were extracted by the reverse spectral fitting tool. The purpose of this study is to explore the difference and quantitative analysis between the diffuse reflectance spectrum and the optical parameters between normal and precancerous lesions tissues.
The system has been approved by the Hospital Scientific Research and Ethics Committee of NTUH. Its electrical safety is also in line with the Ministry of Medical Standards. It is expected that the system can establish the optical parameters and spectral characteristics database of cervical mucosa in the future as one of the basis for judging the process of precancerous lesions.
|Appears in Collections:||生醫電子與資訊學研究所|
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