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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王愛玉 | |
| dc.contributor.author | Wei-Han Shiu | en |
| dc.contributor.author | 徐暐涵 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:38:27Z | - |
| dc.date.available | 2022-08-29 | |
| dc.date.copyright | 2017-08-29 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-07-26 | |
| dc.identifier.citation | 楊芳潔 (2013) 綠竹 BoMSP41 之細胞定位及生化性質探討，碩士論文，國立臺灣大學生化科技學系。
李佩儒 (2014) 重組綠竹 Monocot-Specific protein-41 之純化與性質檢定，碩士論文，國立臺灣大學生化科技學系。 黃塏荔 (2015) 綠竹 BoMSP41 轉殖水稻之建立，碩士論文，國立臺灣大學生化科技學系。 林延翰 (2016) 綠竹 BoMSP41 與其他生物分子交互作用之探討，碩士論文，國立臺灣大學生化科技學系。 Aghdasi B, Ye K, Resnick A, Huang A, Ha HC, Guo X, Dawson TM, Dawson VL, Snyder SH (2001) FKBP12, the 12-kDa FK506-binding protein, is a physiologic regulator of the cell cycle. Proc Natl Acad Sci U S A 98 (5):2425-2430. doi:10.1073/pnas.041614198 Ahn JC, Kim DW, You YN, Seok MS, Park JM, Hwang H, Kim BG, Luan S, Park HS, Cho HS (2010) Classification of rice (Oryza sativa L. Japonica nipponbare) immunophilins (FKBPs, CYPs) and expression patterns under water stress. BMC Plant Biol 10:253. doi:10.1186/1471-2229-10-253 Bai J, Zhu X, Wang Q, Zhang J, Chen H, Dong G, Zhu L, Zheng H, Xie Q, Nian J, Chen F, Fu Y, Qian Q, Zuo J (2015) Rice TUTOU1 encodes a suppressor of cAMP receptor-like protein that Is important for actin organization and panicle development. 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Biochem Biophys Res Commun 345 (2):646-651. doi:10.1016/j.bbrc.2006.04.140 Jinjun Li YY, Zefu Lu, Liusha Yang, Rongcun Gao, Jingen Lu, Jiayang Li and Guosheng Xiong (2012) s Rice 1, encoding a homeodomain protein, regulates trichome development in rice. Kang MY, Kwon HY, Kim NY, Sakuraba Y, Paek NC (2015) CONSTITUTIVE PHOTOMORPHOGENIC 10 (COP10) contributes to floral repression under non-inductive short days in Arabidopsis. Int J Mol Sci 16 (11):26493-26505. doi:10.3390/ijms161125969 Li N, Li Y (2016) Signaling pathways of seed size control in plants. Curr Opin Plant Biol 33:23-32. doi:10.1016/j.pbi.2016.05.008 Li X, Romero P, Rani M, Dunker AK, Obradovic Z (1999) Predicting protein disorder for N-, C-, and internal regions. Genome Inform Ser Workshop Genome Inform 10:30-40 Li Y, Fan C, Xing Y, Jiang Y, Luo L, Sun L, Shao D, Xu C, Li X, Xiao J, He Y, Zhang Q (2011) Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nat Genet 43 (12):1266-1269. doi:10.1038/ng.977 Liu S, Hua L, Dong S, Chen H, Zhu X, Jiang J, Zhang F, Li Y, Fang X, Chen F (2015) OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production. Plant J 84 (4):672-681. doi:10.1111/tpj.13025 Liu WT, Chen PW, Chen LC, Yang CC, Chen SY, Huang G, Lin TC, Ku HM, Chen JJW (2017) Suppressive effect of microRNA319 expression on rice plant height. Theor Appl Genet. doi:10.1007/s00122-017-2905-5 Mao H, Sun S, Yao J, Wang C, Yu S, Xu C, Li X, Zhang Q (2010) Linking differential domain functions of the GS3 protein to natural variation of grain size in rice. Proc Natl Acad Sci U S A 107 (45):19579-19584. doi:10.1073/pnas.1014419107 Rerie WG, Feldmann KA, Marks MD (1994) The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis. Genes Dev 8 (12):1388-1399 Rio DC (2015) Denaturation and electrophoresis of RNA with formaldehyde. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77964 | - |
| dc.description.abstract | BoMSP41 (Monocot specific protein-41 in Bambusa oldhamii) 為綠竹中之未知功能蛋白質。其基因表現量於綠竹較快速生長期的節間中大幅提升，可能參與綠竹生長。為了探討BoMSP41 的功能，先前已建立帶有FLAG-BoMSP41 或 BoMSP41- FLAG 之轉殖水稻(Oryza sativa L. cv Tainung 67)。本研究針對異源表現BoMSP41 對於轉殖株生長之影響進行觀察。比較野生株與轉殖株種子大小，發現轉殖株的種子長度增加，厚度縮小。另外也發現到，轉殖株內外穎上的毛狀體(trichome) 和野生株相比較為稀疏。此外，轉殖株的種子與野生株相比含有較高比例的白堊質。另外，轉殖株的穗長、第二及第三節的節間長度較野生株短，造成植株矮化的性狀。推測於水稻中異源表現BoMSP41 可能參與水稻節間中的細胞增生或細胞延長，毛狀體的分化及種子發育。 | zh_TW |
| dc.description.abstract | BoMSP41 (Monocot specific protein-41 in Bambusa oldhamii) is a protein with unknown function in bamboo. Its gene expression is highly up-regulated in the internode of bamboo during rapid growth stage, suggesting that BoMSP41 may involve in bamboo growth. To get insights into the function of BoMSP41, the FLAG-BoMSP41 or BoMSP41-FLAG gene have been introduced into rice (Oryza sativa L. cv Tainung 67) previously. The effect of ectopic expression of BoMSP41 on rice growth was investigated in this study. Comparing to the wild type, the seeds of transgenic rice showed an increase in seed length but a decrease in seed thickness. Besides, the density of trichomes on the lemma/palea of seed in transgenic plants was less than that of wildtype. Additionally, the seeds of transgenic rice contained higher content of chalkiness than wildtype. Moreover, the length of panicle, the second and the third internodes was decreased in transgenic plants, which resulted in a dwarf phenotype. The results suggest that ectopically expressed BoMSP41 may involve in cell proliferation and/or elongation in internodes, differentiation of trichome and seed development. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:38:27Z (GMT). No. of bitstreams: 1 ntu-106-R04b22044-1.pdf: 2917784 bytes, checksum: d50ec08e06ddcd4e56146437302c9389 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 縮寫表............................................................................................................................1
摘要................................................................................................................................3 Abstract ..........................................................................................................................4 第一章 緒論................................................................................................................5 1.1 研究背景.......................................................................................................................... 5 1.1.1 綠竹中未知功能基因BoMSP41 之發現 .........................................5 1.1.2 BoMSP41 先前研究...........................................................................5 1.1.3 水稻OsMSPs .....................................................................................7 1.2 水稻.................................................................................................................................. 7 1.2.1 水稻種子發育.....................................................................................7 1.2.2 毛狀體(trichome) 形成.....................................................................8 1.2.3 水稻節間延長.....................................................................................9 1.3 研究目的........................................................................................................................ 10 第二章 材料與方法.................................................................................................. 11 2.1 試驗材料........................................................................................................................ 11 2.1.1 水稻...................................................................................................11 2.1.2 藥品...................................................................................................11 2.2 試驗儀器........................................................................................................................ 11 2.2.1 核酸電泳...........................................................................................11 2.2.2 蛋白質電泳.......................................................................................11 2.2.3 離心機...............................................................................................12 2.2.4 即時定量聚合酶鏈鎖反應...............................................................12 2.2.5 掃描式電子顯微鏡...........................................................................12 2.2.6 其他...................................................................................................12 2.3 試驗方法........................................................................................................................ 12 2.3.1 水稻染色體DNA 之抽取與分析...................................................12 2.3.1.1 水稻染色體DNA 之抽取.......................................................12 2.3.1.2 DNA 聚合酶鏈鎖反應...........................................................13 2.3.1.3 DNA 電泳.................................................................................14 2.3.2 mRNA 之分析..................................................................................14 2.3.2.1 total RNA 之抽取.....................................................................14 2.3.2.2 DNase I 處理............................................................................14 2.3.2.3 變性膠體電泳............................................................................15 2.3.2.4 反轉錄聚合酶鏈鎖反應(Reverse transcription-PCR)............15 2.3.2.5 核酸即時定量PCR (quantitative real time PCR, qPCR) ........16 2.3.4 水稻農藝性狀分析...........................................................................18 2.3.4.1 同型合子篩選............................................................................18 2.3.4.2 水稻種子性狀分析....................................................................18 2.3.4.3 水稻株高分析............................................................................18 第三章 結果..............................................................................................................19 3.1 轉殖株基因插入、mRNA表現分析............................................................................ 19 3.1.1 轉殖株BoMSP41 基因插入確認...................................................19 3.1.2 轉殖株BoMSP41 mRNA 表現確認...............................................19 3.1.4 同型合子(homozygote) 篩選.........................................................20 3.2 轉殖株性狀觀察............................................................................................................ 20 3.2.1 水稻種子粒型與種子表面毛狀體分析...........................................20 3.2.1.1. 水稻種子粒型分析...................................................................20 3.2.1.2 水稻種子表面毛狀體分布........................................................21 3.2.2 水稻株高分析...................................................................................22 3.2.3 種子內胚乳之觀察...........................................................................22 第四章 討論..............................................................................................................23 4.1 轉殖株性狀與BoMSP41 表現之關聯性.................................................................... 23 4.2 BoMSP41 可能參與之調控途徑.................................................................................. 24 4.3 BoMSP41 與OsMSP 在演化上之關係..................................................................... 25 第五章 未來展望......................................................................................................26 5.1 種子粒型相關基因表現量分析.................................................................................... 26 5.2 水稻葉片毛狀體型態分析............................................................................................ 26 5.3 胚乳中澱粉含量、組成及澱粉合成相關基因的分析................................................ 26 5.4 OsMSP39 及 OsMSP41 表現圖譜的建立.................................................................. 26 5.5 轉錄體定序.................................................................................................................... 27 Reference .....................................................................................................................28 圖與表..........................................................................................................................32 | |
| dc.language.iso | zh-TW | |
| dc.subject | 白堊質 | zh_TW |
| dc.subject | 株高 | zh_TW |
| dc.subject | 節間延長 | zh_TW |
| dc.subject | 毛狀體 | zh_TW |
| dc.subject | 種子大小 | zh_TW |
| dc.subject | seed size | en |
| dc.subject | trichome | en |
| dc.subject | internode elongation | en |
| dc.subject | chalkiness | en |
| dc.subject | plant height | en |
| dc.title | 異源表現綠竹BoMSP41對於水稻生長及穀粒外觀及品質之影響 | zh_TW |
| dc.title | Effects of ectopic expression of Bambusa oldhamii MSP41 (BoMSP41) on the growth, grain appearance
and quality of rice (Oryza sativa, L.) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 洪傳揚 | |
| dc.contributor.oralexamcommittee | 楊健志,張孟基,蔡育彰 | |
| dc.subject.keyword | 種子大小,毛狀體,節間延長,白堊質,株高, | zh_TW |
| dc.subject.keyword | seed size,trichome,internode elongation,chalkiness,plant height, | en |
| dc.relation.page | 50 | |
| dc.identifier.doi | 10.6342/NTU201701721 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-07-26 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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