請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77827
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Tzu-Hsiang Lin | en |
dc.contributor.author | 林子翔 | zh_TW |
dc.date.accessioned | 2021-07-11T14:35:34Z | - |
dc.date.available | 2022-09-04 | |
dc.date.copyright | 2017-09-04 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-25 | |
dc.identifier.citation | [1] Website: Healthcare.come
[2] 衛生福利部2016全國十大死因 [3] Friederic Parker, JR., 'Frank Burr Mallory, ' The American Journal of Pathology,' Vol. 17, 785-786 (1941). [4] Website: Histology Guide of virtual histology laboratory [5] Maristela L. Onozato, Veronica E. Klepeis, Yukako Yagi, Mari Mino-Kenudson.,' Evaluation of a completely automated tissue-sectioning machine for paraffin blocks,' J Clin Pathol, Vol. 66, 151-154, ( 2013 ) [6] Maristela L. Onozato, Veronica E. Klepeis, Yukako Yagi, Mari Mino-Kenudson., 'A Role of Three-Dimensional (3D)-Reconstruction in the Classification of Lung Adenocarcinoma,' Analytical Cellular Pathology,Vol.35, 79-84 (2012) [7] Pei-Hsun Wu, Anjil Giri, Sean X. Sun, and Denis Wirtz, ' Three-dimensional cell migration does not follow a random walk,' PNAS, Vol. 111, 3949-3954 (2014). [8] Adenocarcinoma. (n.d.), Cancer Treatment Centers of American. Retrieved March 20, 2017, from http://www.cancercenter.com/terms/adenocarcinoma/ [9] R. C. Gonzalez, R. E. Woods, Digital Image Processing second edition, Prentice Hall, 2002 [10] Francesca del Bufalo, Valentina Hoyos,S higeki Yagyu, Ignazio Caruana, Malcolm Brenner., '3D Modeling of Lung Adenocarcinoma With a Hydrogel Scaffold,' Molecular Therapy, Vol. 23, Supplement 1, ( 2015 ) [11] H. C. Berg, 'Random Walks in Biology ,'Princeton University Press, Princeton. ' (1993) [12] Pollard TD, G. G. Borisy , 'Cellular motility driven by assembly and disassembly of actin filaments. ' Cell, Vol.112(4), 453–465 (2003) [13] D. A. Lauffenburger, A. F. Horwitz Cell migration, 'A physically integrated molecular process. ' Cell, Vol.84(3), 359–369. (1996) [14] Pei-Hsun Wu, Anjil Giri, Sean X. Sun, and Denis Wirtz. Three-dimensional cell migration does not follow a random walk . PNAS, March 4, 2014 [15] Jin H, Varner J, 'Integrins: Roles in cancer development and as treatment targets, ' Br J Cancer, Vol.90(3), 561–565 (2004) [16] Wirtz D, Konstantopoulos K, P. C. Searson, 'The physics of cancer: The role of physical interactions and mechanical forces in metastasis, ' Nature Reviews Cancer , Vol.11(7), 512–522 (2011) [17] A. D. Luster, Alon R, U.H. von Andrian, 'Immune cell migration in inflammation: Present and future therapeutic targets, ' Nat Immunol., Vol.6(12), 1182–1190 (2005) [18] Pampaloni, Francesco (October 2007). 'The third dimension bridges the gap between cell culture and live tissue'. Nature Reviews. 8 (10): 839–845. doi:10.1038/nrm2236. Retrieved July 9, 2013. [19] Selection of invasive and metastatic subpopulations from a human lung adenocarcinoma cell line. Am. J. Respir. Cell Mol. Biol. 17:353–360. [20] Turgeon, Mary Louise (2005). Clinical hematology: theory and procedures. Hagerstwon, MD: Lippincott Williams & Wilkins. p. 67. ISBN 0-7817-5007-5. [21] The Journal of Cancer Research October 1 1922 (7) (4) 395-415; DOI: 10.1158/jcr.1922.395 [22] Ya-Lin Huang. “Survey on Lung Adenocarcinoma Cells Nucler-to-Cytoplasm Ratio Analysis for Two-photon Fluorescence Microscopic Images”doi:10.6342/NTU201601628 [23] The study of PLEK2 gene promotes lung cancer cells CL1-0 migration and invasion ability. [24] Website:How to prepare your specimen for IF Microscopy [25] Denk W., Strickler J. H., Webb W. W., 'Two-photon laser scanning fluorescence microscopy,' Science,248,73-6(1990) [26] Yamada, KM (24 August 2007). 'Modeling tissue morphogenesis and cancer in 3D'. Cell. 130 (4): 601–10. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77827 | - |
dc.description.abstract | 癌症,近年來高居全國十大死因之首,而肺癌嚴重性又是更甚於其中。本論文和實驗室同仁主要研究於利用新的三維結構組建平台、3D列印機、實驗室開發的高精準振動切片儀器、雙光子螢光顯微系統,把肺腺癌分化出來的兩種細胞株(Cell lines) CL1-0和CL1-5的腫瘤做三維結構重組和影像分析。過去研究都指向CL1-5較具有侵略性,所以我們希望藉由此論文可以改變傳統癌症上只考量到二維結構的病理結構分析。在近幾年來免疫系統和癌症的交互作用在醫學界中討論的如火如荼,因此癌症分析理論上應該多琢磨考慮三維的細節,此論文以三維影像重組定量分析找出較為適當的討論。建造並提出一整套系統可以將所有關於腦、肺、角膜、皮膚、肝…等等三維結構的影像重組,加上此論文發展出一套新的技術,可以直接把所有組織切片排成矩陣(tissue array)。染色完就可以直接在雙光子激發螢光顯微鏡下觀測,也可以節省大量的人力和時間成本。 | zh_TW |
dc.description.abstract | Cancer is the most common cause of death in Taiwan in recent years and this includes lung cancer. We mainly investigated the use of a new 3-D structure construction platform, a 3-D printer, a high-precision vibrating microtome developed by the laboratory, and a two-photon microscopy system for 3-D structural reconstruction and image analysis of two differentiated lung adenocarcinoma cell lines, CL1-0 and CL1-5. Previous studies have consistently found that CL1-5 is highly invasive. We hope that the findings stated in this thesis can change the conventional 2-D structural pathological analysis of cancers. In recent years, there has been much discussion about the interactions between the immune system and cancer in the medical field; therefore, cancer analysis should theoretically consider 3-D details and we used 3-D image reconstruction and quantitative analysis to identify appropriate discussion points. The constructed and proposed system can carry out 3-D structural reconstruction of the brain, lungs, cornea, skin, liver, and other organs. In addition, a new technology was developed in this study; this technology can directly section all tissues into a tissue array, which can then be directly observed under a two-photon microscope after staining is complete. This can result in significant savings on personnel effort and time. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:35:34Z (GMT). No. of bitstreams: 1 ntu-106-R04245003-1.pdf: 3973088 bytes, checksum: 77fcf13fccd23498942570903125450a (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員會審定書
誌謝 i 中文摘要 iii Abstract iiii CONTENTS v LIST OF FIGURES viii Chapter 1 緒論 1 1.1 研究動機 1 1.2 文獻回顧 2 1.3 組織、器官三維掃描平台開發 5 Chapter 2 實驗原理 6 2.1 肺癌組織介紹 6 2.1.1 呼吸系統 6 2.1.2 惡性腫瘤簡介 8 2.1.3 肺腺癌細胞CL1-0、CL1-5篩選方法 9 2.2 3D列印技術 12 2.2.1 列印塑料PLA 12 2.2.2 裝置原理 12 2.3 非線性光學理論 13 2.3.1 單光子激發光學機制和原理 13 2.3.2 雙光子激發光學機制和原理 18 2.4 免疫螢光抗體染色簡介 21 2.4.1 抗體、抗原批對 21 2.4.2 基本免疫染色步驟 22 Chapter 3 儀器架設、樣品準備和實驗步驟 25 3.1 儀器架設 25 3.1.1 雙光子螢光顯微系統 25 3.2 實驗步驟 26 3.2.1 細胞培養概要 26 3.2.2 CL1-0/CL1-5肺腺癌細胞培養 29 3.2.3 肺腺癌腫瘤培養 30 3.2.4 染色步驟 31 3.2.5 實驗分析方法 32 Chapter 4 結果討論 34 4.1 二維 NC ratio 34 4.2 三維 NC ratio 和Segmentation error 35 4.2.1 Segmentation error – Case1 36 4.2.2 Segmentation error – Case2 36 4.3 比較二維和三維NC ratio 37 4.4 數據結論 38 4.5 缺點改善和限制 39 4.6 三維組織重組開發 39 附錄 42 REFERENCE 48 | |
dc.language.iso | zh-TW | |
dc.title | 雙光子光學顯微造影和高精密切片技術於肺腺癌三維結構影像重組之應用 | zh_TW |
dc.title | Utilizing Two-photon Microscopy and High-precision Cutting Machine to Reconstruct 3D Adenocarcinoma Image | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳惠文(Huei-Wen Chen),陳永芳(Yang-Fang Chen),張顏暉(Yuan-Huei Chang) | |
dc.subject.keyword | 肺腺癌,CL1-0,CL1-5,3D列印機,雙光子螢光顯微術,tissue array, | zh_TW |
dc.subject.keyword | lung adenocarcinoma,CL1-0,CL1-5,3D printer,two-photon microscopy system,tissue array, | en |
dc.relation.page | 49 | |
dc.identifier.doi | 10.6342/NTU201702110 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-08-25 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 應用物理研究所 | zh_TW |
顯示於系所單位: | 應用物理研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-R04245003-1.pdf 目前未授權公開取用 | 3.88 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。