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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉懷勝(Hwai-Shen Liu) | |
| dc.contributor.author | Hsiu-Ling Chen | en |
| dc.contributor.author | 陳秀綾 | zh_TW |
| dc.date.accessioned | 2021-07-10T22:03:39Z | - |
| dc.date.available | 2021-07-10T22:03:39Z | - |
| dc.date.copyright | 2018-08-23 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77472 | - |
| dc.description.abstract | 本實驗的研究目的主要在於探討利用細胞結塊的方式快速、大量地移除水中疏水性汙染物。
本實驗分別使用Rhodococcus erythropolis NTU-1以及未知的微生物NTU-X進行疏水性汙染物移除實驗，所用的兩種疏水性汙染物則為可溶於其他疏水性有機化合物的Sudan I(蘇丹紅)和不易溶於其他疏水性有機化合物的聚乙烯塑膠微粒。 在Sudan I移除實驗中發現，NTU-1和NTU-X皆能在短時間內移除大量的Sudan I：在700 ppm Sudan I的條件下，3天內，90%以上的Sudan I能被移除。移除Sudan I主要是藉由細胞聚集包覆Sudan I再將含有高濃度Sudan I的細胞結塊以物理法撈除，而非利用生物降解分解Sudan I，故能在短時間內移除大量汙染物。 在塑膠微粒移除實驗中，NTU-1和NTU-X也能在短時間內移除大量的塑膠微粒，且對不同粒徑的塑膠微粒也都具有良好的移除效果：700 ppm PE塑膠微粒的條件下，3天內，80%以上的PE塑膠微粒可被移除。其移除原理也是利用細胞聚集包覆PE塑膠微粒再將含有高濃度PE塑膠微粒的細胞結塊以物理法撈除。 利用NTU-1和NTU-X在正十六烷的環境下生長會產生細胞結塊的特性來移除水中疏水性汙染物，結合了物理移除及生物移除法之優點，能在短時間內移除高濃度之汙染物，此法為移除水中疏水性汙染物提供一個相當具競爭及發展性之移除方法。 | zh_TW |
| dc.description.abstract | This thesis focused on utilizing biofloccule formation of R. erythropolis NTU-1 and NTU-X to removal of hydrophobic pollutants in aqueous systems.
In this study, two different kinds of hydrophobic pollutants were chosen. Sudan I, a kind of diazo dye, was used to represent oil-soluble hydrophobic pollutants in aqueous solutions. On the other hand, PE microplastics were selected to represent oil-insoluble hydrophobic pollutants in aqueous solutions. Results showed that both NTU-1 and NTU-X could rapidly remove high concentrations of containments. At least 90% Sudan I and 80% PE microplastics at the concentration of 700 ppm could be removed in 3 days. The main mechanism of removal of hydrophobic compounds is the aggregation of containments and cells, and followed subsequently physical separation. The utilizing of NTU-1 and NTU-X provided a perspective technique that had advantages of both physical and biological methods for remediation of removing hydrophobic compounds in aqueous systems. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T22:03:39Z (GMT). No. of bitstreams: 1 ntu-107-R05524059-1.pdf: 4390776 bytes, checksum: f2a3bfaace262aae85a6ffd139dbbd36 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 摘要…….. I
Abstract.... II 表目錄….. VI 圖目錄….. VII 第一章 緒論 1 1.1 前言 1 1.2 研究目的及論文綱要 2 第二章 文獻回顧 3 2.1 疏水性化合物簡介及其對環境和生態之影響 3 2.1.1 石油碳氫化合物(Petroleum Hydrocarbons, PHCs) 3 2.1.2 多環芳香烴(Polycyclic Aromatic Hydrocarbons, PAHs) 4 2.1.3 多氯聯苯(Polychlorinated Biphenyl, PCBs) 6 2.1.4 疏水性偶氮染料(Azo dye) 7 2.2 處理疏水性汙染物之方法 8 2.3 塑膠微粒介紹和其對環境、生物之影響 11 2.3.1 塑膠微粒介紹 11 2.3.2 塑膠微粒對生物之影響 19 2.4 微生物攝取疏水性碳源之模式 29 2.5 微生物烷類之代謝途徑 34 2.5.1 直鏈烷之氧化機制 34 2.5.2 支鏈烷之氧化機制 37 2.6 實驗菌株Rhodococcus erythropolis介紹 42 2.6.1 Rhodococcus 菌屬簡介 42 2.6.2 Rhodococcus erythropolis之特性及應用 46 2.7 微生物之細胞聚集現象 51 第三章 實驗材料與方法 54 3.1 實驗菌株 54 3.1.1 Rhodococcus erythropolis NTU-1 54 3.1.2 NTU-X 56 3.2 培養基組成與配製 57 3.2.1 液態礦物培養基 57 3.2.2 菌株活化培養基 59 3.2.3 菌株保存培養基 60 3.2.4 菌株篩選培養基 61 3.2.5 計數平板培養基 62 3.3 實驗方法 63 3.3.1 菌株的活化及生長曲線 63 3.3.2 礦物培養基菌液製作 63 3.3.3 Sudan I(蘇丹紅)之移除實驗 64 3.3.4 塑膠微粒之移除實驗 67 3.3.5 分離、篩選、純化NTU-X 70 3.4 實驗藥品與器材 71 3.4.1 實驗藥品 71 3.4.2 實驗儀器 72 第四章 結果與討論 73 4.1 利用NTU-1移除水中Sudan I之汙染 74 4.1.1 NTU-1在含有Sudan I的培養基之生長趨勢 74 4.1.2 NTU-1對不同濃度Sudan I之移除實驗 83 4.2 利用NTU-X移除水中Sudan I之汙染 90 4.2.1 NTU-X在含有Sudan I的培養基之生長趨勢 90 4.2.2 NTU-X對不同濃度Sudan I之移除實驗 96 4.2.3 NTU-X在不同初始培養基pH值之Sudan I移除實驗 102 4.3 NTU-1、NTU-X和其他微生物於Sudan I移除實驗之比較 108 4.4 利用NTU-1移除水中塑膠微粒之汙染 111 4.4.1 NTU-1對不同粒徑塑膠微粒之復育實驗 112 4.4.2 NTU-1對不同濃度塑膠微粒之復育實驗 119 4.5 利用NTU-X移除水中塑膠微粒之汙染 124 4.5.1 NTU-X對不同粒徑塑膠微粒之移除實驗 124 4.5.2 NTU-X對不同濃度粒徑塑膠微粒之復育實驗 132 4.6 NTU-1、NTU-X與其他微生物於塑膠微粒移除實驗之比較 136 第五章 總結 140 參考文獻 143 附錄1 生長曲線 166 附錄2 校正曲線 167 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用微生物細胞聚集現象移除水中疏水性汙染物 | zh_TW |
| dc.title | Removal of Hydrophobic Pollutants in Aqueous Solutions by Microorganism Aggregation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 江佳穎(Chia-Ying Chiang),鄭玉佳(Yu-Chia Cheng) | |
| dc.subject.keyword | NTU-1,NTU-X,疏水性汙染物,Sudan I,聚乙烯塑膠微粒,復育方法, | zh_TW |
| dc.subject.keyword | NTU-1,NTU-X,hydrophobic pollutants,Sudan I,microplastics,remediation, | en |
| dc.relation.page | 169 | |
| dc.identifier.doi | 10.6342/NTU201802552 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2018-08-18 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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