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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李佳音 | zh_TW |
| dc.contributor.advisor | Chia?Yin Lee | en |
| dc.contributor.author | 黃儒音 | zh_TW |
| dc.contributor.author | Ru-Yin Huang | en |
| dc.date.accessioned | 2021-07-10T21:52:29Z | - |
| dc.date.available | 2024-08-15 | - |
| dc.date.copyright | 2019-08-23 | - |
| dc.date.issued | 2019 | - |
| dc.date.submitted | 2002-01-01 | - |
| dc.identifier.citation | Ames, G.F. (1968). Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol 95, 833-843.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77244 | - |
| dc.description.abstract | 腸炎弧菌在台灣是主要引起食物中毒的病原菌之一，磷脂醯絲胺酸合成酶 (Pss) 為弧菌合成磷脂質組成份的一個決定性的酵素，可催化胞苷二磷酸二醯甘油和L型絲胺酸成為磷脂醯絲胺酸及5’-胞核苷單磷酸鹽。本研究欲探討腸炎弧菌中磷脂醯絲胺酸合成酶基因 (pss) 在細菌中的表現及此基因所生成的蛋白質之功能及特性。pss的mRNA於生長的指數期表現，其轉錄起始點位於轉譯起始點上游37個核苷酸，並藉由報導基因的試驗及RNA聚合酶之電泳位移分析證明pss基因的啓動子區域。另外，建構可能的調控者VPA0041之缺陷株發現VPA0041並不能調控pss基因在轉錄層次的表現。此外，由免疫沉澱法及液相串連式質譜儀證明腸炎弧菌的細胞內確實會轉譯出Pss蛋白質。為證實Pss蛋白質具有催化的功能，利用大腸桿菌大量表現可溶的重組Pss，並將其純化進行酵素活性分析。藉由基質輔助雷射解析串聯飛行時間質譜儀，可以證明Pss酵素催化基質後的產物磷脂醯絲胺酸的生成，使用分光光譜儀可以證實Pss酵素催化反應之產物5’-胞核苷單磷酸鹽的產生。另外，Pss最適反應溫度為40℃，最適pH為8，並受到20 mM二價金屬離子的抑制。再者，經由建構Pss的蛋白質立體結構模型，以及將模型與基質進行分子對接的模擬，及定點突變的製作，推測其酵素活性區存在於與其他磷脂酶D家族具保守性的兩個HKD (His(X)Lys(X)4Asp) 區域。弧菌屬中的Pss是具高保守性的蛋白質，本論文研究結果可促進了解其他弧菌之磷脂醯絲胺酸合成酶的基因及生化特性。 | zh_TW |
| dc.description.abstract | Vibrio parahaemolyticus is one of the main food pathogens that cause foodborne illness in Taiwan. Phosphatidylserine synthase (Pss) is a crital enzyme to synthesize phospholipid, which can catzlyze cytidine diphosphate diacylglycerol and L‐serine to form phosphatidylserine and cytidine 5’‐ monophosphate. This study would investigate the gene expression of the phosphatidylserine synthase gene (pss) in V. parahaemolyticus, and the function and the characteristics of Pss. pss mRNA was found to be expressed mainly during the exponential phase. The transcriptional start site of pss was mapped through sequencing and was identified at −37 nucleotides upstream of the start codon. In addition, the promoter was identified using a lux reporter assay and gel shift assay with an RNA polymerase. Furthermore, one predicted Pss regulator deletion mutant ΔVPA0041 was constructed, however, VPA0041 was found that it was no effect on regulating the transcriptional level of pss. To analyze the catalytic activity, a soluble form of His6‐tagged recombinant Pss was overexpressed and purified from Escherichia coli. Using matrix‐assisted laser desorption ionizationtime of flight mass spectrometry, the formation of phosphatidylserine was proved. The production of cytidine 5’‐monophosphate was confirmed by using spectrophotometer. Moreover, the optimum temperature of Pss was 40°C and the optimum pH of Pss was 8. The activity of Pss was inhibited by 20 mM divalent metal. In addition, through the construction of the three-dimensional structure of Pss, the simulation of molecular docking of the model with the substrate, and site-directed mutagenesis, the active region of the enzyme is presumed to be the two HKD (His(X)Lys(X)4Asp) regions conserved with other phospholipase D families. Since Pss is a conserved protein in Vibrio, this study can help to understand genetic and biochemical characterization of Pss in other Vibrio. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:52:29Z (GMT). No. of bitstreams: 1 ntu-108-D00623005-1.pdf: 4968581 bytes, checksum: ffaa4ce98d4f88a452da1861a1917556 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii 英文摘要 iv 目錄 vi 表次 ix 圖次 x 附錄表次 xii 附錄圖次 xiii 縮寫表 xiv 第一章 前言 1 一、文獻回顧 1 1. 弧菌 1 2. 腸炎弧菌 1 3. 磷脂質 3 4. 磷脂醯絲胺酸合成酶 5 5. 磷脂醯絲胺酸合成酶調控者PssR 6 二、研究動機 8 第二章 pss基因分析 9 摘要 9 一、前言 9 二、材料與方法 9 1. 菌株、質體及引子 9 2. 生物資訊工具 9 3. RNA萃取 10 4. 即時定量反轉錄聚合酶連鎖反應(qRT-PCR) 11 5. 轉錄起始點之分析 11 6. 建構luxAB訊號載體 12 7. 電穿孔轉形 (electroporation) 12 8. 冷光強度之測定 13 9. 建構RpoD表現載體 13 10. 表現及純化RpoD重組蛋白質 13 11. 電泳位移分析法（Electrophoretic Mobility Shift Assay，EMSA） 14 12. 建構VPA0041缺陷株 15 13. 南方雜合 16 14. 統計分析 17 三、結果 17 1. 腸炎弧菌的Pss是第一型的PS合成酶 18 2. pss基因的表現與生長的時期相關 18 3. 鑑定pss基因的啓動子 18 4. VPA0041不能調控pss基因的轉錄 19 四、討論 20 五、結論 21 第三章 Pss蛋白質的表現及酵素活性分析 22 摘要 22 一、前言 22 二、材料與方法 23 1. 菌株、質體及引子 23 2. 建構myc-pss菌 23 3. 純化Myc-Pss 24 4. 西方雜合 24 5. 胰蛋白酶膠內酶解 (In gel trypsin digestion) 25 6. 建構Pss表現載體 25 7. 表現及純化重組Pss 26 8. MALDI-TOF質譜分析酵素活性 27 9. Cytidine 5’ ‐ monophosphate (CMP) 標準曲線的製作 28 10. Pss最適反應溫度測試 28 11. Pss最適反應pH測試 28 12. 測試二價金屬離子對Pss的影響 28 13. 測試CDP‐DAG (18:1/18:1) 是否可作為Pss的基質 29 三、結果 29 1. 鑑定腸炎弧菌會轉譯出Pss蛋白質 29 2. 腸炎弧菌重組蛋白質於大腸桿菌內表現及純化 30 3. MALDI‐TOF質譜儀分析酵素活性 30 4. 紫外光分光光度計分析Pss酵素活性 31 四、討論 31 五、結論 32 第四章 Pss結構分析 33 摘要 33 一、前言 33 二、材料與方法 33 1. 菌株、質體及引子 33 2. 模擬Pss之蛋白質立體結構 33 3. 分析Pss酵素的活性區 34 4. 模擬Pss酵素與基質CDP-DAG(16:0)分子對接 34 5. 序列排比分析弧菌屬的Pss 34 6. 製作Pss HKD區之Histidine定點突變 34 7. 定點突變Pss酵素H131A及H352Ａ之Pss活性分析 35 三、結果 35 1. 建構Pss的三維蛋白質模型並模擬與基質分子對接 35 2. Pss中的兩個HKD 區的兩個Histidine對於酵素活性是重要的 36 四、討論 36 五、結論 36 總結 38 未來展望 39 參考文獻 79 附錄表 96 附錄圖 97 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 磷脂醯絲胺酸 | zh_TW |
| dc.subject | 5’-胞核?單磷酸鹽 | zh_TW |
| dc.subject | 磷脂醯絲胺酸合成? | zh_TW |
| dc.subject | 磷脂質 | zh_TW |
| dc.subject | 磷脂?D家族 | zh_TW |
| dc.subject | 腸炎弧菌 | zh_TW |
| dc.subject | Vibrio parahaemolyticus | en |
| dc.subject | phospholipid | en |
| dc.subject | phosphatidylserine synthase | en |
| dc.subject | phosphatidylserine | en |
| dc.subject | cytidine 5’?monophosphate | en |
| dc.subject | phospholipase D family | en |
| dc.title | 腸炎弧菌磷脂醯絲胺酸合成酶基因及生化特性之研究 | zh_TW |
| dc.title | The genetic and biochemical characterization of phosphatidylserine synthase in Vibrio parahaemolyticus | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 107-2 | - |
| dc.description.degree | 博士 | - |
| dc.contributor.oralexamcommittee | 羅禮強;陳翰民;劉啟德;余美萱 | zh_TW |
| dc.contributor.oralexamcommittee | Lee-Chiang Lo;Han-Min Chen;Chi-Te Liu;Mei?Shiuan Yu | en |
| dc.subject.keyword | 腸炎弧菌,磷脂質,磷脂醯絲胺酸合成?,磷脂醯絲胺酸,5’-胞核?單磷酸鹽,磷脂?D家族, | zh_TW |
| dc.subject.keyword | Vibrio parahaemolyticus,phospholipid,phosphatidylserine synthase,phosphatidylserine,cytidine 5’?monophosphate,phospholipase D family, | en |
| dc.relation.page | 102 | - |
| dc.identifier.doi | 10.6342/NTU201902760 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2019-08-14 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 農業化學系 | - |
| 顯示於系所單位： | 農業化學系 | |
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