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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 鄭石通(Shih-Tong Jeng) | |
dc.contributor.author | Cheng-Chung Huang | en |
dc.contributor.author | 黃承中 | zh_TW |
dc.date.accessioned | 2021-07-10T21:35:47Z | - |
dc.date.available | 2021-07-10T21:35:47Z | - |
dc.date.copyright | 2016-10-14 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-07-29 | |
dc.identifier.citation | Adams, D.R., Ron, D., and Kiely, P.A. (2011). RACK1, A multifaceted scaffolding protein: Structure and function. Cell Commun. Signal. 9: 22.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76728 | - |
dc.description.abstract | 阿拉伯芥基因RELATED to AP2 6 (RAP2.6;AT1G43160),為APETALA2/Ethylene Responsive Factor (AP2/ERF)轉錄因子家族中的一員,先前已在多個報告中被認為會參與各種生物性及非生物性逆境相關的反應調控。它同時也是基於COI1之茉莉酸誘導的轉錄因子,並且參與植物對抗微生物入侵的早期反應。在此研究中,阿拉伯芥RAP2.6基因表現量在植株受到葉片機械性傷害以及處理甲基茉莉酸後皆顯著地受到誘導。另外,RAP2.6表現量受到內生性一氧化氮以及外部施加的亞硝基谷胱甘肽(S-nitrosoglutathione,GSNO,一種一氧化氮釋放劑)抑制,但是RAP2.6卻在一種缺乏一氧化氮突變株noa1之中被誘導,顯示NOA1可能透過與一氧化氮不相關的未知路徑調控RAP2.6表現量。在機械性傷害處理之後,部分參與茉莉酸生合成的基因以及反應基因其表現量在野生型植株以及rap2.6低表現量(knock-down)突變株中皆上升,但是在野生型中的上升量遠大於突變株中的上升量。這些結果顯示RAP2.6在由茉莉酸誘導的傷害反應訊息傳遞路徑之中,扮演正回饋調控因子的角色。Receptor for Activated C Kinase 1 A (RACK1A)是阿拉伯芥中一個參與植株發育與免疫反應的架構蛋白,其功能為固定MAPK cascade 反應中的元件與G蛋白中的Gβ次單元結合。在先前研究中顯示,水稻OsRACK1A可與OsRAP2.6 (OsERF113)結合。在此研究中透過酵母菌雙雜合系統顯示RACK1A與RAP2.6會交互作用反應,表示RACK1A可能參與尚未確認的RAP2.6轉錄後調控機制。 | zh_TW |
dc.description.abstract | The Arabidopsis RELATED to AP2 6 (RAP2.6; AT1G43160), one of the APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors, has been reported to participate in both biotic and abiotic stresses. RAP2.6 is also a COI1-dependent jasmonate (JA)-inducible transcription factors, which take parts in early plant defense responses to microorganism. In this study, RAP2.6 was significantly induced after either leaves mechanical wounding or methyl jasmonate (MeJA) treatment. RAP2.6 was suppressed by endogenous nitric oxide (NO) and exogenous S-nitrosoglutathione (GSNO), a NO donor, but was induced in one of a NO deficient mutant line noa1. These results, suggested that NOA1 may regulate RAP2.6 expressions in a NO-independent pathway. After mechanical wounding, expression levels of several genes responsible for JA biosynthesis and JA response were induced in both wild type and rap2.6 knock-down plants. However, expression levels in wild type were higher than those in mutant plants, suggesting RAP2.6 participates in JA-mediated wounding signaling pathway as a positive feedback regulator. Arabidopsis Receptor for Activated C Kinase 1 A (RACK1A) is a scaffold protein which is involved in plant development and immune responses by recruiting MAPK cascade components and interacting with heterotrimeric G protein Gβ subunit. In previous study, OsRACK1A had shown binding affinity with OsRAP2.6 (OsERF113) in rice. In this study, Arabidopsis RACK1A was shown to interact with RAP2.6 by yeast two hybrid assay. This may suggest that RACK1A participates in a putative RAP2.6 post-translational regulation. | en |
dc.description.provenance | Made available in DSpace on 2021-07-10T21:35:47Z (GMT). No. of bitstreams: 1 ntu-105-R03b42001-1.pdf: 1283530 bytes, checksum: 33166f5fd7a86e19d13db065f20ab30f (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | Thesis Oral Defense Committee Report I
謝詞 (Acknowledgements) II 摘要(Chinese Abstract) III Abstract IV Content V Appendix Content VIII Chapter I. Introduction 1 Chapter II. Materials and methods 7 1. Plant materials and growth conditions 7 2. Plant treatment and harvesting 7 3. Expression levels analysis 8 3-1. Total RNA extraction 8 3-2. DNase treatment 8 3-3. Reverse transcription-PCR (RT-PCR) 9 3-4. Quantitative real-time PCR (qPCR) 9 4. Genomic DNA extraction 10 5. Plasmid constructing 11 5-1. PCR (polymerase chain reaction) 11 5-2. DNA gel electrophoresis 12 5-3. DNA gel extraction 12 5-4. DNA fragment ligation 13 5-5. Competent cells transformation and incubation 13 5-6. E. coli plasmid mini extraction 14 5-7. DNA sequencing 15 6. Yeast two hybrid 15 6-1. Yeast strain maintenance 15 6-2. Yeast competent cells preparation 16 6-3. Yeast transformation 16 6-4. Yeast selection 17 Chapter III. Results 21 1. Expression of transcription factors altered in noa1 upon wounding 21 2. Expression levels of RAP2.6 transcription factors raise rapidly upon wounding 21 3. Expression levels of RAP2.6 inhibited by NO but enhanced by NOA1 22 4. RAP2.6 expression levels was rapidly enhanced upon mechanical wounding and MeJA treatment 23 5. rap2.6-2 is a T-DAN insertion RAP2.6 knock-down mutant line 23 6. JA-responsive genes expression is less sensitive in rap2.6-2 than wild type upon mechanical wounding 24 7. Prediction of cis-elements of JA biosynthesis-related genes with RAP2.6 binding site 24 8. Expression of JA biosynthesis-related genes are less sensitive in rap2.6-2 than wild type under mechanical wounding 25 9. RAP2.6 interacts with RACK1A in vivo 25 Chapter IV. Discussion 26 1. RAP2.6 expression level is inhibited by NO, but induced by NOA1 26 2. RAP2.6 is a wounding- and JA-responsive gene, but not relate to wounding-induced NO 26 3. RAP2.6 participates in JA-mediated wound signal transduction 27 4. Putative RAP2.6 post-translational regulations through RACK1 and MAPK cascade 28 5. Integrating RAP2.6 into NO signaling and JA- mediated wounding response pathway 28 Chapter V. Reference 30 Appendix 37 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥中AP2/ERF轉錄因子RAP2.6在傷害反應中的角色 | zh_TW |
dc.title | Role of AP2/ERF Transcription Factor RAP2.6 in Arabidopsis thaliana Responding to Wounding | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林讚標(Tsan-Piao Lin),林詩舜(Shih-Shun Lin),邱子珍(Tz-Jen Chiou),黃浩仁(Hao-Jen Huang) | |
dc.subject.keyword | RAP2.6,一氧化氮,茉莉酸,正回饋,RACK1A,酵母菌雙雜合系統, | zh_TW |
dc.subject.keyword | RAP2.6,nitric oxide,jasmonate,positive feedback,RACK1A,yeast two hybrid, | en |
dc.relation.page | 50 | |
dc.identifier.doi | 10.6342/NTU201601115 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2016-07-29 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
Appears in Collections: | 植物科學研究所 |
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