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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱清良(Ching-Liang Chu) | |
| dc.contributor.author | Guei-Yun Cheng | en |
| dc.contributor.author | 鄭貴云 | zh_TW |
| dc.date.accessioned | 2021-05-19T17:49:22Z | - |
| dc.date.available | 2022-09-12 | |
| dc.date.available | 2021-05-19T17:49:22Z | - |
| dc.date.copyright | 2017-09-12 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-20 | |
| dc.identifier.citation | Akira Sekikawa, et al. Involvement of the IL-22/REG Iα axis in ulcerative colitis. Laboratory Investigation. 2010 January; 90, 496–505.
Baumgart DC, Sandborn WJ. Crohn's disease. Lancet. 2012 Nov 3;380(9853): 1590-605. Brian D., et al. Metal Chelation and Inhibition of Bacterial Growth in Tissue Abscesses. Science. 2008 Feb; 319 (5865): 962-965 Chen HY, et al. Role of galectin-3 in mast cell functions: galectin-3-deficient mast cells exhibit impaired mediator release and defective JNK expression. J Immunol. 2006 Oct 15;177(8):4991-7. Cho KA, et al. Interleukin-17 and Interleukin-22 Induced Proinflammatory Cytokine Production in Keratinocytes via Inhibitor of Nuclear Factor κB Kinase-α Expression. Ann Dermatol. 2012 Nov;24(4):398-405. Cummings RD, Liu FT. Galectins. Essentials of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009. Chapter 33. Danese S, Fiocchi C. Ulcerative colitis. N Engl J Med. 2011 Nov 3;365(18):1713-25. Dessein R, et al. TLR2 is critical for induction of Reg3 β expression and intestinal clearance of Y. pseudotuberculosis. Gut. 2009 Jun;58(6):771-6. Eva Hainzl, et al. Intestinal Epithelial Cell Tyrosine Kinase 2 Transduces IL-22 Signals To Protect from Acute Colitis. J Immunol. 2015 Nov 15; 195(10): 5011–5024. Fukui H, et al. DMBT1 is a novel gene induced by IL-22 in ulcerative colitis. Inflamm Bowel Dis. 2011 May;17(5):1177-88. Geetha Srikrishna. S100A8 and S100A9: New Insights into Their Roles in Malignancy. J Innate Immun. 2011 Dec; 4(1): 31–40. Geethanjali Pickert, et al. STAT3 links IL-22 signaling in intestinal epithelial cells to mucosal wound healing. J Exp Med. 2009 Jul 6; 206(7): 1465–1472. Hsu DK, et al. Targeted disruption of the galectin-3 gene results in attenuated peritoneal inflammatory responses. Am J Pathol. 2000 Mar;156(3):1073-83. Janet Z. Liu, et al. Zinc sequestration by the neutrophil protein calprotectin enhances Salmonella growth in the inflamed gut. Cell Host Microbe. 2012 Mar 15; 11(3): 227–239. Jensen-Jarolim E, et al. The constitutive expression of galectin-3 is downregulated in the intestinal epithelia of Crohn's disease patients, and tumour necrosis factor alpha decreases the level of galectin-3-specific mRNA in HCT-8 cells. Eur J Gastroenterol Hepatol. 2002 Feb;14(2):145-52. Judith Behnsen, et al. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity. 2014 Feb 20; 40(2): 262–273. Lejeune D, et al. Interleukin-22 (IL-22) activates the JAK/STAT, ERK, JNK, and p38 MAP kinase pathways in a rat hepatoma cell line. Pathways that are shared with and distinct from IL-10. J Biol Chem. 2002 Sep 13;277(37):33676-82. Lim C, Savan R. The role of the IL-22/IL-22R1 axis in cancer. Cytokine Growth Factor Rev. 2014 Jun;25(3):257-71. Lörchner H, et al. Myocardial healing requires Reg3β-dependent accumulation of macrophages in the ischemic heart. Nat Med. 2015 Apr;21(4):353-62. Michelle M. Averill, et al. S100A8 and S100A9 in Cardiovascular Biology and Disease. Arterioscler Thromb Vasc Biol. 2012 Feb; 32(2): 223–229. Midwood KS, et al. Tissue repair and the dynamics of the extracellular matrix. Int J Biochem Cell Biol. 2004 Jun;36(6):1031-7. Mitra A, et al. IL-22 induced cell proliferation is regulated by PI3K/Akt/mTOR signaling cascade. Cytokine. 2012 Oct;60(1):38-42. Nata K, et al. Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III. Gene. 2004 Sep 29;340(1):161-70. Perusina Lanfranca M, et al. Biological and pathological activities of interleukin-22. J Mol Med (Berl). 2016 May;94(5):523-34. Pham TA, et al. Epithelial IL-22RA1-mediated fucosylation promotes intestinal colonization resistance to an opportunistic pathogen. Cell Host Microbe. 2014 Oct 8;16(4):504-16. Pickert G, et al. STAT3 links IL-22 signaling in intestinal epithelial cells to mucosal wound healing. J Exp Med. 2009 Jul 6;206(7):1465-72. Raffatellu M, et al. Simian immunodeficiency virus-induced mucosal interleukin-17 deficiency promotes Salmonella dissemination from the gut. Nat Med. 2008 Apr;14(4):421-8. Schreiber F, et al. Pathogen Resistance Mediated by IL-22 Signaling at the Epithelial-Microbiota Interface. J Mol Biol. 2015 Nov 20;427(23):3676-82. Sohini Mukherjee and Lora V. Hooper. Antimicrobial Defense of the Intestine. Immunity. 2015 Jan; 42(1): 28-39, January Theodore Kouo, et al. Galectin-3 shapes antitumor immune responses by suppressing CD8+ T cells via LAG-3 and inhibiting expansion of plasmacytoid dendritic cells. Cancer Immunol Res. 2015 Apr; 3(4): 412–423. Tsai HF, et al. Galectin-3 suppresses mucosal inflammation and reduces disease severity in experimental colitis. J Mol Med (Berl). 2016 May;94(5):545-56. Wolk K, et al. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur J Immunol. 2006 May;36(5):1309-23. Zheng Y, et al. Interleukin-22 mediates early host defense against attaching and effacing bacterial pathogens. Nat Med. 2008 Mar;14(3):282-9. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7661 | - |
| dc.description.abstract | Galectin-3 (Gal-3) 在生理反應上調控許多生物活性功能。在腸炎症患者中，腸道中的Gal-3 表現量明顯較健康人低。在腸道發炎情況下，Interleukin-22 (IL-22) 能透過表現抗菌蛋白，如Reg3β/3γ 及 S100A8/A9，來保護腸道避免遭受病原菌侵犯。IL-22結合到IL-22受器後，會活化下游Jak1/Tyk2-STAT3、Akt 及 MAPK 訊號路徑。許多研究顯示 Gal-3 及 IL-22 對於維持腸道生理平衡扮演重要角色，故我們假設 Gal-3 也許參與在IL-22/IL-22R 訊號傳遞，並調控腸表皮細胞的功能。我們研究中發現，Gal-3似乎不參與在IL-22/IL-22R下游主要的三條訊號路徑。在動物實驗的大腸組織中，雖然Gal-3不參與在調控 Reg3b/3g 的表現，但Gal-3會負調控 IL-22 所誘導表現的 S100A8/A9。 | zh_TW |
| dc.description.abstract | Galectin-3 (Gal-3) is known to exert a lot of biological function. Expression of Gal-3 is significantly downregulated in the gut of patients with inflammatory bowel disease (IBD). Interleukin-22 (IL-22) is known to protect against colitis through enhancing the production of antimicrobial peptides (AMP), such as Reg3β/3γ and S100A8/A9. Binding of IL-22 to its receptor activates Jak1/Tyk2-STAT3, Akt and MAPK signaling pathways. It has been reported that Gal-3 and IL-22 are important to maintain the intestinal homeostasis. We hypothesize that Gal-3 may participate in IL-22 signaling and regulate the functions in intestinal epithelial cells (IECs). In our study, Gal-3 seems not regulate the three major IL-22 signaling pathways. Although Gal-3 is not involved in regulating Reg3b/3g expression, it negatively regulates IL-22-induced S100A8/A9 expression in colon. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-19T17:49:22Z (GMT). No. of bitstreams: 1 ntu-106-R04449010-1.pdf: 4682627 bytes, checksum: fe2839ab91988b8ebd675c96b080bc72 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 III Abstract IV 一、 前言 1 1. Inflammatory bowel disease (IBD) 1 2. Galectin (Gal) 2 2.1 Galectins 簡介 2 表一、Galectins 依結構分類 2 表二、Galectins 在人類與小鼠的分布情形 2 2.2 Galectin-3 (Gal-3) 3 3. Interleukin-22 (IL-22) 4 4. 抗菌蛋白 (antimicrobial peptides/proteins, AMPs) 5 4.1 Reg3β/3γ 5 4.2 S100A8/A9 5 二、材料與方法 7 1. 細胞培養 7 1.1 CMT-93 (Mouse intestinal epithelial cell line) 7 1.2 HT-29 (human intestinal epithelial cell line) 7 2. 動物實驗 7 2.1 操作與培養步驟 7 2.2 組織處理配方 8 3. Knockdown 8 4. Real-time PCR 9 5. 西方墨點法 (Western Blotting) 9 6. 統計分析 10 三、結果 11 1. Galectin-3 普遍表現在 IEC 11 2. 細胞生長密度不影響 galectins profile 11 3. IL-22 不影響腸表皮細胞galectins 的表現分布 11 4. Gal-3 也許不參與在IL-22 所誘導的 AMPs之表現 in vitro 12 5. Gal-3 不影響 IL-22/IL-22R axis 的主要訊號傳遞 13 6. Gal-3 會影響 IL-22Ra1 的表現 ex vivo 13 7. Gal-3缺失不影響 IL-22 所誘導的 Reg3β/γ 之表現 ex vivo 14 8. Gal-3 缺失會促進IL-22 所誘導的 S100A8/A9 之表現 in vivo 14 四、討論 15 五、圖表 18 六、參考文獻 29 七、附件 i 附件一：名稱縮寫 i 附件二：Lgals3 shRNA sequence ii 1. Mouse ii 2. Human ii 附件三：試劑 iv 附件四：Real-time PCR Primers v 1. Mouse v 2. Human vi 附件五：實驗動物鼠品系與資訊 vii | |
| dc.language.iso | zh-TW | |
| dc.title | 半乳糖凝集素-3與介白素-22訊號傳遞對於腸表皮細胞表現抗菌胜肽之影響 | zh_TW |
| dc.title | The Effect of Galectin-3 and IL-22 Signaling on Antimicrobial Peptides Gene Expression in Intestinal Epithelial Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 繆希椿(Shi-Chuen Miaw),徐志文(Jr-Wen Shui) | |
| dc.subject.keyword | 半乳糖凝集素-3,介白素-22,炎症性腸病,抗菌胜?,Reg3b,Reg3g,S100A8,S100A9, | zh_TW |
| dc.subject.keyword | galectin-3,Interleukin-22,nflammatory bowel disease,antimicrobial peptides,Reg3b,Reg3g,S100A8,S100A9, | en |
| dc.relation.page | 39 | |
| dc.identifier.doi | 10.6342/NTU201704125 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2017-08-20 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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