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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
| dc.contributor.author | Po-Hang Tseng | en |
| dc.contributor.author | 曾柏杭 | zh_TW |
| dc.date.accessioned | 2021-05-19T17:49:06Z | - |
| dc.date.available | 2022-09-03 | |
| dc.date.available | 2021-05-19T17:49:06Z | - |
| dc.date.copyright | 2017-09-03 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-24 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7652 | - |
| dc.description.abstract | 前腦發育畸形症屬稀罕人腦疾病，胎兒發育期間會發展出不正常的腦室，可依據不同的腦室變形程度加以分類。得此病最嚴重的情況胎兒只會有一個腦室，無法順利發育雙眼與鼻子。通常胎兒發育期間最先會由醫學超音波檢查診斷出該疾病，在最嚴重的情況下出生後無法存活。根據病理學，研究異常人體器官取下之組織樣本時須將組織切片並且由光學顯微鏡在小於釐米尺度的範圍觀察切片的細節。在本研究中，我們利用連續石蠟切片染色後由高解析度白光顯微鏡分析組織切片，並試著比較正常與前腦發育畸形之胎兒腦組織在二維及三維結構上的差異。另外我們也希望透過新型振動式切片技術與雙光子顯微技術的結合發展出更好的腦組織三維完整取像方法。使用任何方式進行組織切片時都會有組織損失的問題，進而增加三維影像接合之變數，本文亦將探討此問題對三維結構重建之影響。 | zh_TW |
| dc.description.abstract | Holoprosencephaly, a kind of brain disease, is usually identified by ultrasonography on fetuses during gestation and confirmed by Magnetic Resonance Imaging (MRI). In the most severe cases the fetus carries craniofacial malformations Cyclopia and Proboscis above the eye with the nose absent.
In this research we use the standard histological method to study the microscopic anatomy of brain tissue from human postmortem fetal brain with the Holoprosencephaly disease. Consecutive paraffin slices stained with Hematoxylin and Eosin were imaged by light microscopy and the images were then reconstructed into 3D stacks for more information on the anatomy of the disease. We also study the possibility of using agarose for embedding brain tissue along with the oscillating blade cutting technique to obtain better 3D image from slices with two-photon microscopy. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-19T17:49:06Z (GMT). No. of bitstreams: 1 ntu-106-R04222054-1.pdf: 3013484 bytes, checksum: be3560001738c54f12485efb2736f3df (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 iii Abstract iv CONTENTS v LIST OF FIGURES viii LIST OF TABLES x Chapter 1 Introduction 1 1.1 Motivation 1 1.2 Experimental Approach 3 Chapter 2 The Brain 5 2.1 Anatomical Reference 5 2.2 Basic Structure 7 2.2.1 Cerebrum 7 2.2.2 Cerebral Cortex and Layering 8 2.2.3 Hippocampus 10 2.2.4 Corpus Callosum 11 2.2.5 The Ventricular System 12 2.3 Congenital Brain Disease and Holoprosencephaly 13 2.3.1 Anencephaly 14 2.3.2 Lissencephaly 14 2.3.3 Holoprosencephaly 15 Chapter 3 The Standard Histology Approach 18 3.1 Brain Histology and Histopathology 18 3.1.1 Fixation 20 3.1.2 Paraffin Embedding 21 3.1.3 Tissue Sectioning with Microtome 22 3.1.4 Hematoxylin and Eosin Staining 23 3.1.5 Light Microscopy 24 3.2 Methods 26 3.2.1 Sample Preparation 26 3.2.2 Experimental Setup for Light Microscopy 27 3.2.3 Experiment Procedure 28 3.3 Results and Discussion 29 3.3.1 Histological Findings from 2-D Images 29 3.3.2 3-D Analysis of 20 Consecutive Tissue Slices 32 3.3.3 Discussion 41 Chapter 4 The Fresh tissue Approach 43 4.1 Overview of Alternative Histology 43 4.1.1 Agarose 44 4.1.2 The Pig Brain 45 4.2 The Flexure-Based Oscillating Blade System 46 4.2.1 Design of the modified vibrating Microtome 46 4.2.2 Cutting Parameters 48 4.2.3 Blade Material 48 4.2.4 Surface Roughness 51 4.3 Two-Photon Microscopy 52 4.3.1 Fluorescence 52 4.3.2 Two-Photon Fluorescence 54 4.3.3 Fluorescent Dyes and DAPI 55 4.4 Methods 57 4.4.1 Sample preparation 57 4.4.2 Setup of the Oscillating Blade System and Two-Photon LSM 58 4.4.3 Experiment Procedure 60 4.5 Results and Discussion 61 4.5.1 Steel Blade 62 4.5.2 Sapphire Blade 63 4.5.3 Tungsten Carbide Blade 64 4.5.4 Discussion 64 Chapter 5 Conclusion 66 5.1 Comparison of the Histology Techniques 66 5.2 Present Limitations and Future Work 67 REFERENCE 69 | |
| dc.language.iso | en | |
| dc.title | 利用組織切片技術研究腦組織三維結構 | zh_TW |
| dc.title | Survey on Brain Tissue 3-D Reconstruction using different Histology techniques | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 黃佩欣(Pei-Hsin Huang) | |
| dc.contributor.oralexamcommittee | 陳永芳(Yang-Fang Chen),張顏暉(Yuan-Huei Chang) | |
| dc.subject.keyword | 前腦發育畸形症,振動式切片技術,組織病理學,人腦,雙光子顯微技術, | zh_TW |
| dc.subject.keyword | Holoprosencephaly,Oscillating Microtome,Human Fetal Brain,Histopathology,Two Photon Microscopy, | en |
| dc.relation.page | 71 | |
| dc.identifier.doi | 10.6342/NTU201704179 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2017-08-25 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 物理學研究所 | zh_TW |
| 顯示於系所單位： | 物理學系 | |
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