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標題: | 柑桔潰瘍病原菌非致病突變株之特性分析 Characterization of a non-pathogenesis mutant of Xanthomonas campestris pv. citri induced by Tn5tac1 |
作者: | 王浩文 |
出版年 : | 1997 |
學位: | 碩士 |
摘要: | 柑桔潰瘍病原菌(Xanthomonas campestris pv. citri)是一種植物致病菌,會造成柑桔類植物的柑桔潰瘍病。利用轉位子Tn5tac1誘導X. c. pv. citri突變得到的突變株XT-906無病徵表現,是屬於無致病能力(non-pathogenicity, NP)的突變株。 本研究以XT-906(NP)突變株為研究材料,分離被Tn5tac1破壞的致病相關基因:首先建立野生型X. c. pv. citri(XW47)與突變株XT-906的基因庫;再利用Tn5tac1為標記,由突變株基因庫中篩選出帶有Tn5tac1及部分X. c. pv. citri DNA片段的選殖株。進一步在野生型基因庫中篩選到包含此致病相關基因片段的選殖株。而後建立此DNA片段的限制脢圖譜及定出部分DNA序列;另外,測定突變株XT-906細胞外分泌性酵素的活性,包括:澱粉水解脢、蛋白質分解脢及果膠分解脢,發現和野生型XW47並無差異。 Xanthomonas campestris pv. citri (X. c. pv. citri) is a phytopathogenic bacterium resulting the citrus canker disease. A non-pathogenic mutant, XT-906, of X. c. pv. citri was isolated by Tn5tac1 insertional mutagenesis. To study the pathogenesis regulation of XT-906, genomic libraries of wild type X. c. pv. citri and XT-906 were constructed. The Tn5tac1 containing clone was initially isolated from the XT-906 library. DNA fragments surrounding the transposon were determined and used as the probe to further isolate gene(s) conferring to the non-pathogenic phenotypes of XT-906 from the wild type library. Secretary enzymes such as amylase, protease and pectate lyase of XT-906 have been detected. However there is no significant difference in the activity of these extracellular enzymes between the XT-906 mutant and the wild type Xanthomonas. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76395 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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