請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76359完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Hong-Wen Guo | en |
| dc.contributor.author | 郭鴻文 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:40Z | - |
| dc.date.available | 2021-07-01T08:20:40Z | - |
| dc.date.issued | 1998 | |
| dc.identifier.citation | 賴明龍（1993）國立台灣大學生化科學研究所博士論文 Aarons, D., Boettger-Tong, H., Holt, G. and Poirier, G. R. (1991) Mol. Reprod. Dev. 30, 258-64 Aarons, D., Speake, J. L. and Poirier, G. R. (1984) Biol. Reprod. 31, 811-7 Baldi, E., Krausz, C., Luconi, M., Bonaccorsi, L., Maggi, M. and Forti, G. (1995) J. Steroid Biochem. Mol. Biol. 53, 199-203 Bayer, E. A. and Wilchek, M. (1990) Methods in Enzymology 184, 138-160 Bedford, J. M. (1994) Hum. Reprod. 9, 842-858 Bedford, J. M., Moore, H. D. M. and Franklin, L. E. (1979) Exp. Cell Res. 119, 119-126 Benoff, S. (1993) Hum. Reprod. 8, 2001-2006 Bleil, J. D. and Wassarman, P. M. (1990) Proc. Natl. Acad. Sci. USA 87, 5563-5567 Bleil, J. D. and Wassarman, P. M. (1988) Proc. Natl. Acad. Sci. USA 85, 6778-6782 Boettger-Tong, H., Aarons, D., Biegler, B., Lee, T. and Poirier, G. R. (1992) Biol. Reprod. 47, 716-722 Calvete, J. J., Solis, D., Sanz, L., Diaz-Maurino, T. and Topfer-Petersen, E. (1994) Biol. Chem. Hoppy-Seyler 375, 667-673 Chang, M. C. (1951) Nature 168, 697-698 Chen, Y. H., Pentecost, B. T., McLachlan, J. A. and Teng, C. T. (1987) Mol. Endocrinol. 1, 707-16 Cheng, A., Le, T., Palacios, M., Bookbinder, L. H., Wassarman, P. M., Suzuki, F. and Bleil, J. D. (1994) J. Cell Biol. 125, 867-878 Coronel, C. E., Winnica, D. E., Novella, M. L. and Lardy, H. A. (1992) J. Biol. Chem. 267, 20909-15 Coronel, C. E., San Agustin, J. T. and Lardy, H. A. (1988) Biol. Reprod. 38, 713-722 Drickamer, K. and Taylor, M. E. (1993) Annu. Rev. Cell Biol. 9, 237-264 Florman, H. M. and Wassarman P. M. (1985) Cell41, 313-324 Fraser, L. R. (1995) Hum. Reprod. 10, 22-30 Fraser, L. R. (1992) Arch. Pathol. Lab. Med. 116, 345-350 Fraser, L. R. (1985) J. Reprod. Fertil. 74, 185-96 Garber, D. L. (1989) Annu. Rev. Biochem. 58, 719-742 Greve, J. M. and Wassarman, P. M. (1985) J. Mol. Biol. 181, 253-264 Horii, A., Tomita, N., Yokouchi, H., Doi, S., Uda, K., Ogawa, M., Mori, T. and Matsubara, K. (1989) Biochem. Biophys. Res. Commun. 162, 151-9 Irwin, M., Nicholson, N., Haywood, J. T. and Poirier, G. R. (1983) Biol. Reprod. 28, 1201-6 Jones, R. (1990) J. Reprod. Suppl. 42, 89-105 Kalab, P., Visconti, P., Leclerc, P. and Kopf, G. S. (1994) J. Biol. Chem. 269, 3810-3817 Lai, M. L., Chen, S. W. and Chen, Y. H. (1991) Arch. Biochem. Biophys. 290, 265-71 Lee, S. L. and Wei, Y. H. (1993) Biotechnol. Appl. Biochem. 19, 31-40 Leyton, L., LeGuen, P., Bunch, D. and Saling, P. M. (1992) Proc. Natl. Acad. Sci. USA 89, 11692-11695 Leyton, L. and Saling, P. (1989) Cell. 57, 1123-30 Mcleskey, S. B., Dowds, C., Carballada, R., White, R. R. and Saling, P. M. (1998) Int. Rev. Cyt. 177, 57-113 Mills, J. S., Needham, M., Parker and M. G. (1987a) EMBO J. 6, 3711-7 Mills, J. S., Needham, M., Thompson, T. C. and Parker, M. G. (1987b) Mol. Cell. Endocrinol. 53, 111-8 Mortillo, S. and Wassarman. P. M. (1991) Development 113, 141-139 O'Rand, M. G. (1988) Gamete Res. 19, 315-328 Ostrowski, M. C., Kistler, M. K. and Kistler, W. S. (1979) J. Biol. Chem. 254, 383-90 Oyen, O., Mvklebust, F., Scott, J. D., Hansson, V. and Jahnsen, T. (1989) FEBS Lett. 246, 57-64 Page, M., Baines, M. G. and Thorpe, R. (1994) Methods in Molecular Biology (J. M. Wallers, ed.) 32, pp. 407-432 Phelps, B. M., Primakoff, P., Koppel, D. E., Low, M. G. and Myles, D. G. (1988) Science 240, 1780-1782 Poirier, G. R. and Jackson, J. (1981) Gamete Res. 4, 555-569 Richardson, K. T., Yamasaki, N., and O Rand, M. G. (1994). Dev Biol. 165, 688-701 Robert, M. and Gagnon, C. (1995) Human Reprod. 10, 2192-7 Rosiere, T. K. and Wassarman, P. M. (1992). Dev. Biol. 154, 309-317 Sacco, A. G., Yurewicz, E. C., Subramanian, M. G., and Matzat, P. D. (1989) Biol. Reprod. 41, 523-532 Saling, P.M. (1981) Pro. Natl. Acad. Sci. USA, 78, 6231-5 Sanz, L., Clavete, J. J., Jonakova, V. and Topfer-Petersen, E. (1992) FEBS Lett. 300, 63-66 Sanz, L., Calvete, J. J., Mann, K., Schafer, W., Schmid, E. R. and Topfer-Petersen, E. (1991) FEBS Lett. 291, 33-36 Schagger, H. and von Jagow. G. (1987) Anal. Biochem. 166, 368-379 Shur, B. D. and Hall, N. G. (1982) J. Cell Biol. 95, 574-579 Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985) Anal. Biochem. 150, 76-85 Tanaka, N., Espey, L. L., Stacy, S. and Okamura, H. (1992) Biol. Reprod. 46, 665-670 Topfer-Petersen, E. (1996) Reprod. Domest. Anim. 31, 93-100 Topfer-Petersen, E. and Calvete, J. J. (1995) Int. J. Androl. 18, 20-26 Topfer-Petersen, E., Calvete, J. J., Sanz, L. and Sinowatz, F. (1995) Andrologia 27, 303-324 Topfer-Petersen, E., Steinberger, M., von Eschenbach, C. E. and Zucker, A. (1990) FEBS Lett. 265, 51-54 Topfer-Petersen, E., and Henschen, A. (1988) Biol. Chem. Hoppe-Seyler 369, 69-76 Tschesche, H., Wittig, B., Decker, G., Muller-Esterl, W. and Fritz, H. (1982) Eur. J. Biochem. 126, 99-104 Tsubamoto, H., Hasegawa, A., Inoue, M., Yamasaki. N. and Koyama, K. (1996) J. Reprod. Fertil. 50, 63-67 Urch, U. A., and Patel. H. (1991). Development 111, 1165-1172 Veselsky, L., Jonakova, V., Sanz, M. L., Topfer-Petersen, E. and Cechova, D. (1992) J. Reprod. Fertil. 96, 593-602 Veselsky, L., Cechova, D. and Jonakova, V. (1989) Int. J. Fertil. 34, 231-4 Vihko, K. K., Suominen, J. J. and Parvinen, M. (1984) Biol. Reprod. 31, 383-9 Ward, C. R. and Storey, B. T. (1984) Dev. Biol. 104, 287-296 Wassarman, P. M. and Litscher, E. S. (1995) Curr. Top. Dev. Biol. 30, 1-19 Wilson, J. D. (1978) Annu. Rev. Physiol. 40, 279-306 Yanagimachi, R. (1981) Fertilization and Embryonic Development In Vitro (L. Mastroianni, and J. D Biggers, eds.) pp. 81-187 Yonezawa, N., Aoki, H,. Hatanaka, Y. and Nakano, M. (1995) Eur. J. Biochem. 233, 35-41 Yurewicz, D. C., Pack, B. A., Armant, D. R. and Sacco, A. G. (1993) Mol. Reprod. Dev. 36, 382-389 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76359 | - |
| dc.description.abstract | 前人之研究而知，從小白鼠貯精囊分泌液純化不含醣鏈的胰蛋白?抑制因數（Irypsin Inhibitor，簡稱TI）屬Kazal-type proteinase inhibitor，其分子量為6.4 kDa，只存在雄性生殖系統中貯精囊、前列腺及凝固腺（coagulating gland）等部位。探討TI對精蟲活性的影響為本論文之研究重點，使用間接螢光檢定法及直接的組織染色法來觀察TI結合在精蟲上的位置，可以發現TI會結合在精蟲頭端的頂體（acrosome）位置。由chlortetracycline （CTC）螢光染色分析和Coomassie Blue （CB）染色的方法研究而知，TI可抑制由牛血清白蛋白（bovine serum albumin）所誘發精細胞產生獲能作用（capacitation）及頂體反應（acrosome reaction）的能力。此外，使用冷熱的方式（frozen-thawed method）所得到溶解性的精蟲蛋白，通過TI親和性管柱，可得一個分子量約為15kDa的蛋白，而此蛋白可能為TI在精細胞上的結合蛋白。 | zh_TW |
| dc.description.abstract | A kazal-type trypsin inhibitor (TI) has been previously purified from seminal vesicle secretion in our laboratory. The protein contains no carbohydrate and its molecular weight is 6.4 kDa as estimated by SDS-electrophoresis. Among the reproductive tracts of mice, TI is product only male accessory sexual gland such as seminal vesicles, prostate and coagulating gland. This work aimed to assess the effect of TI on sperm activity. Moreover, TI could suppressed the bovine serum albumin induced sperm capacitation and acrosome reaction as assayed by the chlortetracycline (CTC) fluorescence method and the Coomassie Blue (CB) staining technique. A protein with a Mr around 15 kDa could be isolated from the supernatant of frozen-thawed epididymal sperm suspension through an affinity column of agrose linked with TI. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:40Z (GMT). No. of bitstreams: 0 Previous issue date: 1998 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………1 英文摘要……………………………………………………2 簡寫表……………………………………………………3 論文內容……………………………………………………4 圖 次……………………………………………………31 參考文獻……………………………………………………48 | |
| dc.language.iso | zh-TW | |
| dc.title | 小白鼠貯精囊自體中胰蛋白?抑制因數的生理研究 | zh_TW |
| dc.title | Physiological Study of a Trypsin Inhibitor from Mouse Seminal Vesicle Secretion | en |
| dc.date.schoolyear | 86-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 50 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。