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|Title:||淡水長臂大蝦大顎器官離體培養下類青春激素 MF 之合成與調控|
In vitro synthesis and regulation of methyl farnesoate in mandibular organ of Macrobrachium rosenbergii
|Publication Year :||1997|
|Abstract:||甲殼類類青春激素MF(methyl farnesoate）是由大顎器官(mandibular organ, MO）所合成與分泌。本報告以淡水長臂大蝦為實驗對象，建立大顎器官之離體培養系統，以TLC、GC/MS、HPLC等層析法定性分析大顎器官離體培養下的產物MF。其結果證明所取組織可分泌MF，表示MO解剖位置無誤，而MF之定量分析則以放射性化學分析法（Radiochemical Assay, RCA）進行，實驗中雌性淡水長臂大蝦之MF合成速率約為3.0±1.3pmol/hr/MO。|
It is established that the mandibular organ (MO) of decapod crustaceans synthesizes and secretes methyl farnesoate (MF). In this report, MOs of the giant freshwater prawn, Macrobrachium rosenbergii were investigated by in vitro incubation system. Products of MO were identified by TLC, GC/MS and HPLC. Secretion of MF from the dissected MOs confirmed the site of MO in M rosenbergii. Radiochemical assay (RCA) was used to quantify in vitro MF synthesis. The rate of MF synthesis in female M. rosenbergii was 3.0±1.3 pmol/hr/MO.
With regard to the regulation of MF synthesis, the synthetic potential of MF on the third day of unilaterally eyestalk ablation was about 2.3 times greater than that of intact. On the other hand, this effect was reduced by addition of crude eyestalk extract in the medium. These results suggested that MF synthesis might be modulated by neuropeptides produced and released from the X-organ sinus gland complex of the eyestalk. As to the intracellular signal transduction pathway of the regulation, in vitro treatment of MO with 8-Br-cAMP (cAMP analog), 8-Br-cGMP (cGMP analog), forskolin (adenylate cyclase activator) and SNP (sodium nitroprusside, guanylate cyclase activator) all had inhibitory effect on MF synthesis. Therefore, cAMP and cGMP might act as second messengers in the regulation of MF synthesis. According to these in vitro data, the synthesis of MF should be under some precise regulation and this MO bioassay system can be a useful tool for studies on neurohormonal regulation and its signal transduciton.
|Appears in Collections:||漁業科學研究所|
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