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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳雅鈴 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:13Z | - |
| dc.date.available | 2021-07-01T08:20:13Z | - |
| dc.date.issued | 1997 | |
| dc.identifier.citation | Aasland, R. , Gibson, T.J. , and Stewart, A.F.(1995) The PHD finger: implications for chromatin-mediated transcriptional regulation. Trends Biochem. Sci. 20:56-59.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76316 | - |
| dc.description.abstract | 核受體屬於配基-誘發性的轉錄因數，其在與認知的反應區結合後可調節標的基因的表現；核受體的配基-依賴性活化功能，AF-2 ，推測可經由轉錄介子/中間因數作用在基本的轉錄機構上。雖然中間因數(介子)顯現居間傳導核受體的活化功能，但是對於這個由核受體引發的轉錄性活化的分子機轉仍不瞭解。筆者由小鼠基因庫中篩選到 TIF1β這個核蛋白，並證實它在試管中可與醣皮激素受體交互作用。在哺乳動物細胞，予以配基刺激，可促進小鼠乳腺腫瘤病毒-和α1-酸性醣蛋白-氯黴素乙醯基轉移?基因的表現。因此TIF1β很可能是一個有關媒介醣皮質激素受體荷爾蒙結合區活化功能的共活子。 | zh_TW |
| dc.description.abstract | Nuclear receptors (NRs) act as ligand-inducible transcription factors which regulate the expression of target genes upon binding to their cognate response elements. Their ligand-dependent activation function, AF-2, presumably acts on the basal transcription machinery through transcriptional mediators/intermediary factors (TIFs). But the molecular mechanisms underlying transcriptional activation by NRs are still poorly understood, although intermediary factors (mediators) appear to be involved in mediating the transactivation functions of NRs. We have isolated the cDNA encoding mouse nuclear proteins, TIF1β, that can interact with the glucocorticoid receptor (GR) in vitro. Overexpression of the TIF1β gene in mammalian cells enhances the hormone-regulated expression of mouse mammary tumor virus- and α1-acid glycoprotein- chloramphenical acetyltransferase gene. Thus TIF1β can probably serve as a coactivator, potentiating the transactivation functions in glucocorticoid receptor hormone binding domain (HBD). | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:13Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 英文摘要………………………………………………………………………………………………………………………1 中文摘要………………………………………………………………………………………………………………………2 第一章、緒論(Introduction)………………………………………………………………………………………………3 第二章、材料與方法(Materials and Methods) …………………………………………………………………………8 一、質體之抽取 ………………………………………………………………………………………………………8 二、製備勝任細胞與細胞轉型 ………………………………………………………………………………………9 三、質體的構築 ………………………………………………………………………………………………………11 四、在大腸桿菌中表現融合蛋白質 …………………………………………………………………………………12 五、鎳離子親和性管柱層析法 ………………………………………………………………………………………12 六、SDS聚丙烯醯胺膠體電泳…………………………………………………………………………………………14 七、蛋白質R9單源及多源抗體的製備 ………………………………………………………………………………15 八、西方墨點法 ………………………………………………………………………………………………………16 九、DNA探針之放射性標記……………………………………………………………………………………………17 十、細胞RNA之抽取……………………………………………………………………………………………………17 十一、北方墨點分析 …………………………………………………………………………………………………18 十二、多種組織之北方墨點分析 ……………………………………………………………………………………20 十三、從老鼠基因庫篩選出TIFlβ的基因 …………………………………………………………………………23 十四、細胞培養 ………………………………………………………………………………………………………24 十五、免疫螢光細胞染色法 …………………………………………………………………………………………24 十六、細胞轉染 ………………………………………………………………………………………………………25 十七、氯黴素乙醯基轉換?分析 ……………………………………………………………………………………26 十八、全細胞液之抽取 ………………………………………………………………………………………………27 十九、免疫沉澱法 ……………………………………………………………………………………………………27 第三章、結果…………………………………………………………………………………………………………………29 第四章、討論…………………………………………………………………………………………………………………35 參考文獻………………………………………………………………………………………………………………………42 圖表……………………………………………………………………………………………………………………………49 圖一、Modular structural and functional organization of nuclear receptors with particular emphasis on the activation function2(AF-2). 圖二、Schematic representation of the mouse mammary tumor virus long terminal repeat sequence. 圖三、AGP基因promoter上的蛋白質結合位 圖四、R9基因片段嵌在pT3T7D載體上的質體圖譜 圖五、R9基因片段以RcoRI/HindIII限制?位嵌在pRSET-B表現之質體圖譜 圖六、大腸桿菌轉型菌株表現融合蛋白質R9的情形 圖七、利用SDS聚丙烯醯胺膠體電泳分析以鎳離子親和性管柱純化出來的融蛋白質R9 圖八、以北方墨點法分析RN?2.9kb大小的TIFlβ在不同的細胞株(A)與各種人類組織(B)中的表現 圖九、TIFlβ是核內蛋白質，散佈在細胞核每個角落且有集結的現象 圖十、以1％洋菜膠體電泳分析以R9為探針從小鼠基因庫篩選全長的TIFlβ基因 圖十一、TIFlβ的DNA序列 圖十二、在HeLa細胞株中，TIFlβ可促進結合ligand後之GR對MMTV基因活性 圖十三、在HeLa細胞株中，TIFlβ的超表現促進結合ligand後之GR對MMTV基因的活性 圖十四、在HeLa細胞株中，ligand的濃度會影響TIFlb/GR複合體對MMTV基因的活性 圖十五、In vitro interaction of TIFlb with the glucocorticoid receptor. 圖十六、調節AGP基因表現的模型 圖十七、Diagram of the vectors used to transfect BHK cells. 圖十八、在BHK細胞株中，TIFlβ可促進結合ligand後之GR對AGP基因的活性 圖十九、在BHK細胞株中，TIFlβ的超表現促進結合ligand後之GR對AGP基因的活性 圖二十、在BHK細胞株中，改變AGP promoter之GRE site抑制TIFlβ/GR對AGP基因的活化 圖二十一、在BHK細胞株中，改變AGP promoter之C區後TIFlβ/GR對AGP基因調控的情形 圖二十二、在BHK細胞株中，改變AGP promoter之D區後TIFlβ/GR對AGP基因調控的情形 圖二十三、在BHK細胞株中，改變AGP promoter之D區後TIFlβ/GR對AGP基因調控的情形 圖二十四、在BHK細胞株中，改變AGP promoter之CE區後TIFlβ/GR對AGP基因調控的情形 圖二十五、在BHK細胞株中，改變AGP promoter之CDE區後TIFlβ/GR對基因調控的情形 圖二十六、在BHK細胞株中，TIFlβ可促進AGP/EBP對AGP基因的活化 | |
| dc.language.iso | zh-TW | |
| dc.title | TIF1β在α1-酸性醣蛋白基因調控之研究 | zh_TW |
| dc.title | Regulation of α1-Acid Glycoprotein Gene by TIFlβ | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 86 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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