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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 鍾宜書 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:11Z | - |
| dc.date.available | 2021-07-01T08:20:11Z | - |
| dc.date.issued | 1997 | |
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Antibodies against Ku protein in sera from patients with autoimmune diseases. Clin. Exp. Immunol. 76: 366-372 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76313 | - |
| dc.description.abstract | 本論文研究一段新的基因，它所encode的protein具有PHD/ LAP finger，命名為Requiem 6，文中除了研究細胞中它的messenger RNA及蛋白質表現情形之外，也找到了全長cDNA，同時發現Requiem 6與DNA-dependent protein kinase catalytic subunit (DNA-PKcs)存在於同一個complex中。 同樣會與 DNA-dependent protein kinase (DNA-PKcs)形成complex的Ku protein早在1985年就知道它是自體抗原（autoantigen）。而DNA-PKcs是1996年才被發現為自體抗原，同時發現DNA-PKcs自體抗體與Ku protein自體抗體的存在，兩者之間有正相關性。本論文在氣喘(asthma)、全身性硬化症、疑似palindromic syndrome、紅斑性狼瘡(SLE)、乾性角膜結合膜炎（Sj?gren's syndrome）、類風濕關節炎(rheumatoid arthritis)這類自體免疫病患中測得Requiem 6自體抗體，而有Requiem 6自體抗體的病人會伴隨著DNA-PKcs自體抗體存在，卻不一定有Ku protein自體抗體。 | zh_TW |
| dc.description.abstract | cDNA encoding a PHD Finger protein, Requiem 6, was cloned. The expression of Requiem 6 mRNA was found in many tissues and cells .Requiem 6 is associated with a complex containing DNA-dependent protein kinase catalytic subunit (DNA-PKcs). Recently, patients have been identified with antibodies to the DNA-PKcs that appears to be associated with anti-Ku antibody. Anti-Requiem 6 auto-antibody was found to be present in patients of systemic lupus erythematosus, Sj?gren's syndrome, rheumatoid arthritis, asthma, palindromic syndrome and multiple sclerosis. The appearance of anti-Requiem 6 and anti-DNA-PKcs auto-antibodies in patients with autoimmune conditions is strikingly similar. These results suggest that Requiem 6 and DNA-PKcs exist in the same complex. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:11Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 縮寫表…………………………………………………………………………………1 英文摘要………………………………………………………………………………2 中文摘要………………………………………………………………………………3 第一章、緒論…………………………………………………………………………4 第二章、材料與方法…………………………………………………………………9 一、抽取質體……………………………………………………………………9 二、SDS聚丙烯醯 凝膠電泳……………………………………………………10 三、質體建構……………………………………………………………………11 四、表現融合蛋白質……………………………………………………………12 五、鎳離子親和性管柱純化融合蛋白質………………………………………12 六、西方墨點法…………………………………………………………………13 七、細胞培養……………………………………………………………………14 八、製備全細胞萃取物…………………………………………………………14 九、南方墨點法…………………………………………………………………15 十、篩選 HeLa cDNA Library…………………………………………………16 十一、免疫沉澱法………………………………………………………………17 十二、DNA定序法 ………………………………………………………………18 第三章、結果…………………………………………………………………………20 第四章、討論…………………………………………………………………………16 參考文獻………………………………………………………………………………31 圖一……………………………………………………………………………………37 圖二……………………………………………………………………………………38 圖三……………………………………………………………………………………39 圖四……………………………………………………………………………………39 圖五……………………………………………………………………………………40 圖六……………………………………………………………………………………41 圖七……………………………………………………………………………………42 圖八……………………………………………………………………………………43 圖九……………………………………………………………………………………45 圖十……………………………………………………………………………………46 圖十一…………………………………………………………………………………47 表一……………………………………………………………………………………48 表二……………………………………………………………………………………49 | |
| dc.language.iso | zh-TW | |
| dc.title | 一 PHD finger 蛋白質的 cDNA 選殖及其在自體免疫病人自體抗體之偵測 | zh_TW |
| dc.title | Cloning of a cDNA Encoding a Protein with PHD Finger and Detection of Its Autoantibody in Autoimmune Disease Patients | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 56 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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