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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林睿軒 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:05Z | - |
| dc.date.available | 2021-07-01T08:20:05Z | - |
| dc.date.issued | 1997 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76304 | - |
| dc.description.abstract | 本論文是以水生無脊椎動物斑節蝦的眼為材料，藉以勝?受質KLKCFFL的酵素活性檢測方法，先後經過HiTrap?Q陰離子交換管柱層析，Superdex TM200膠體過瀘管柱層析，Mono Q陰離子交換管柱層析，最後以勝?偶合親合力管柱完成蛋白質牻牛兒牻牛兒基轉移?I (PGGT I)的純化。純化的PGGT I比活性為188 units/mg。從膠體過濾管柱層析估算的分子量為67 kDa與SDS-PAGE所估算的66 kDa相近，推測斑節蝦蛋白質牻牛兒牻牛兒基轉移?I為單體結構。所得的蛋白質被抗法呢基轉移?α次單元的抗體(Y-53)所辨認，顯示二者含有相似的胺基酸序列。 在所檢測的勝?中，KCFFL是較好的勝?受質。酵素作用最適的酸鹼值是pH 8.0。斑節蝦蛋白質牻牛兒牻牛兒基轉移?I證實是一種金屬性?（EDTA的I.C.50=0.25μM）但活性會受Mg2+（I.C.50=500μM）及Zn2+ (I.C.50=50μM）所抑制。根據各組織的酵素活性檢測，廣布於斑節蝦的眼、肝胰臟、心臟、鰓、神經節、腸、肌肉及殼各組織。 | zh_TW |
| dc.description.abstract | Protein geranylgeranyltransferase I was purified from the eyes of shrimp Penaeus japonicus by the sequentail column chromatography on HiTrap? Q, Superdex? 200, Mono Q and peptide-coupled affinity column. The purified enzyme was found to have relative Mr of 66 kDa as estimated by SDS-PAGE. Since the active protein geranylgeranyltransferase I from Superdex? 200 was found to have relative mass of 67 kDa, the purified enzyme was deduced to be a monomer. Synthetic peptide KCFFL is the best substrate of the protein geranylgeranyltransferase I among the investigated peptides. The optimal pH for enzyme activity is pH 8.0. The enzyme was inhibited by Mg++ and Zn++ ions at 50μM and 500μM respectively. Since EDTA (IC50=0.25μM) is inhibitory as well, the protein geranylgeranyltransferase I of Penaeus japonicus is a metaloenzyme. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:05Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 中文摘要 1 英文摘要 2 緒言 3 材料與方法 13 材料 一．生物材料 13 二．合成勝? 13 三．試劑與藥品 13 四．反應組與器材 15 方法 一．斑節蝦牻牛兒牻牛兒基轉移? I 之純化 17 二．蛋白質牻牛兒牻牛兒基轉移? I 之活性分析 22 三．膠體過濾層析法估算分子量 24 四．矽膠薄層層析 25 五．蛋白質的定量 26 六．硫酸十二酯鈉聚丙烯醯胺膠體電泳 27 七．銀染色法 28 八．西方免疫點墨分析 28 結果 30 討論 37 參考文獻 43 圖表 54 | |
| dc.language.iso | zh-TW | |
| dc.title | 斑節蝦眼的蛋白質牻牛兒牻牛兒基轉移?I之純化與定性 | zh_TW |
| dc.title | Purification and Characterization of Protein Geranylgeranyltransferase I from the Eyes of the Shrimp Penaeus japonicus | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 82 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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