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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Ju-Ling Lin | en |
| dc.contributor.author | 林如玲 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:54Z | - |
| dc.date.available | 2021-07-01T08:19:54Z | - |
| dc.date.issued | 1997 | |
| dc.identifier.citation | 張奕元（1995）絲瓜果實韌皮部蛋白質的純化、鑑定與定位。國立台灣大學植物研究所碩士論文 莊榮輝（1985）水稻蔗糖合成之研究。國立台灣大學農業化學研究所博士論文 莊榮輝、蘇仲卿（1987）蛋白質膠體電泳檢定法。電泳分離技術研討會論文集，行政院國家科學委員會發行。69-85 陳益明（1987）蛋白質的雙向電泳分離法。電泳分離技術研討會論文集，行政院國家科學委員會發行。59-67 王銘川（1996）七美絲瓜果實溢流42.5kDa蛋白質純化、鑑定與免疫定位。國立台灣大學植物研究所碩士論文 Aitken, A., Geisow, M., Findlay, J.B., Holmes, C., Yarwood, A. (1989) Peptide preparation and characterization. In: Protein sequencing -a practical approach. Findlay, J.B.C. and Geisow, M.J. (eds). IRL press, Oxford England, pp. 43-68 Allen, A.K. (1979) A lectin from the exudate of the fruit of the vegetable marrow (Cucurbita pepo) that has specificity for β-1,4-linked N-acetylglucosamine oligosaccharides. Biochem. J. 183: 133-137 Alosi, M.C., Melroy, D.L., Park, R.B. (1988) The regulation of phloem exudate from Cucurbita fruit by dilution, glutathione and glutathione reductase. Plant Physiol. 86: 1089-1094 Baba, K., Ogawa, M., Nagano, A., Kuroda, H., Sumiya, K. (1991) Developmental changes in the bark lectin of Sophora japonica L. Planta 183: 462-470 Behnke, H.D, Sjolund, R.D. (1990) Sieve Elements: Comparative Structure, Induction and Development. Springer-Verlag, Berlin. pp. 266-283 Behnke, H.D., Kiritsis, U. (1983) Ultrastructure and differentiation of sieve elements in primitive angiosperms. I. Winteraceae. Protoplasma 118: 148-156 Bentwood, B. J., Cronshaw, J. (1978) Cytochemical localization of adenosine triphosphatase in the phloem of Pisum sativum and its relation to the function of transfer cells. Planta 140: 111-120 Bentwood, B.J, Cronshaw, J. (1976) Biochemistry and cytochemical localization of acid phosphatase in the phloem of Nicotiana tabacum. Planta 130: 97-104 Beyenbach, J, Weber, C, Kleinig, H. (1974) Sieve tube proteins from Cucurbita maxima. Planta 119: 113-124 Bostwick, D.E., Dannenhoffer, J.M., Skaggs, M.R., Larkins, B.A., Thompson, G.A. (1992) Pumpkin phloem lectin genes are specifically expressed in companion cells. 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Planta 118: 85-87 Gaffney, T., Friendrich, L., Vernooij, B., Negrotto, D., Nye, G., Uknes, S., Ward E., Kessmann, H., Ryals, J. (1993) Requirement of salicylic acid for the induction of systemic acquired resistance. Science 261:754-756 Garvey, J.S., Cremer, N.E., Susdorf, D. H. (1977) Methods in Immunology: A Laboratory Text for Instruction and Research, W.A. Benjamin, Northampton, Mass Geitl, C., Kauss, H., Ziegler, H. (1979) Affinity chromatography of a lectin from Robinia pseudoacacia L. and demonstration of lectins in sieve tube sap from other tree species. Planta 144: 367-372 Gilder, J., Cronshaw, J. (1973a) The distribution of adenosine triphosphatase activity in differentiating and mature phloem cells of Nicotiana tabacum and its relationship to phloem transport. J. Ultrastruct. Res. 44: 388-404 Gilder, J., Cronshaw, J. (1973b) Adenosine triphosphatase in the phloem of Cucurhita. Planta 110: 189-204. Gilder, J., Cronshaw, J. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76287 | - |
| dc.description.abstract | 植物韌皮部存在著一群複雜具組織專一性的蛋白質，唯至今對P-protein生化功能的瞭解仍很有限。本實驗室於前年從絲瓜果實的韌皮部溢流液中得到24kDa蛋白質的抗體，本實驗則分別利用SDS-PAGE及雙向電泳，對七美絲瓜果實的韌皮部溢流液及中長絲瓜的植株進行蛋白質之分離及免疫轉印分析，同時將其中的24kDa溢流蛋白純化，進行胺基酸組成分析及N-端序列定序。在SDS-PAGE電泳圖譜上，七美絲瓜果實溢流蛋白主要可以分為4群，而藉由免疫轉印的分析得知七美絲瓜的24kDa溢流蛋白可被抗體所辨識，除了24kDa的主帶外，還有少許其他蛋白質被辨識。在雙向電泳圖譜中，絲瓜果實溢流液以酸性蛋白居多，而在免疫墨漬分析上，可辨識一群分子量為24kDa，pI為4-5.2的蛋白（約有4-6個點）。此外，24kDa蛋白群在三天大的小苗中即被表現，並且存在木質部中。將其中一個分子量24kDa，pI 5.2的溢流蛋白純化，經胺基酸組成分析得知其組成以Glycine、Aspartic acid及Glutamic acid居多，分別占12.3％、8.9％及7.2％，對此蛋白質進行CNBr的斷裂反應，而後送至中部貴儀中心進行N-端序列分析。 | zh_TW |
| dc.description.abstract | P-proteins are groups of protein expressed specifically in the phloem. Their biological function have been not well defined so far. A polyclonal antibody against 24 kDa phloem protein was prepared in our previous study. In the present study, phloem proteins from Luffa cylindrica were separated with SDS-PAGE and 2-D electrophoresis. There are four groups of phloem protein in SDS-PAGE profile. A major band at 24 kDa and some minor bands can be identified by the polyclonal antibody. Most fruit phloem exudate protein are acid in 2-D gel. Immunoblotting study showed that 4-6 spots at 24 kDa with pI ranging from 4 to 5.2 are detected. The antibody of pepo 24 kDa also recognized the similar proteins expressed in a three-day-old seedling and in xylem sap. A 24 kDa protein with pI 5.2 obtained from 2-D gel have 16 kinds of amino acids with high contain of Glycine (12.3%) Aspartic acid (8.9%) and Glutamic acid (7.2%). The N-terminal sequencing is totally blocked. Further cleavage of 24 kDa by CNBr was tested and peptide fragments were transferred to PVDF membrane for sequence analysis. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 圖表目錄………………………………………………………………………………………iii 中文摘要………………………………………………………………………………………iv 英文摘要………………………………………………………………………………………v 壹、前言………………………………………………………………………………………1 貳、材料與方法………………………………………………………………………………7 植物材料………………………………………………………………………………………7 （一）蛋白質樣品的收集……………………………………………………………………7 （二）蛋白質的定量…………………………………………………………………………8 （三）蛋白質的分離…………………………………………………………………………9 （四）Glycoprotein的染色…………………………………………………………………17 （五）24kDa P-Protein的免疫分析…………………………………………………………19 （六）24kDa P-Protein的純化………………………………………………………………21 （七）HPLC蛋白質純度分析…………………………………………………………………22 （八）胺基酸組成分析………………………………………………………………………23 （九）24kDa蛋白的序列分析…………………………………………………………………23 參、結果………………………………………………………………………………………25 （一）七美絲瓜果實韌皮部溢流蛋白的SDS膠體電泳分析及免疫轉印分析………………25 （二）七美絲瓜果實韌皮部溢流蛋白的原態梯度電泳分析及免疫轉印分析……………26 （三）溢流液中Glycoprotein的檢定………………………………………………………26 （四）檢視24kDa蛋白在絲瓜幼苗的表現情形………………………………………………27 （五）24kDa蛋白在木質部溢流液存在之檢視………………………………………………27 （六）七美絲瓜果實韌皮部溢流蛋白的雙向電泳分析……………………………………28 （七）果實韌皮部溢流蛋白與莖部總萃取蛋白的雙向電泳及免疫分析…………………29 （八）24kDa蛋白質的純化……………………………………………………………………29 （九）胺基酸水解分析及蛋白質N-端定序…………………………………………………30 肆、討論………………………………………………………………………………………58 伍、參 考 文 獻………………………………………………………………………………64 | |
| dc.language.iso | zh-TW | |
| dc.title | 七美絲瓜果實之24kDa溢流蛋白的分離、純化與定序 | zh_TW |
| dc.title | Isolation, Purification and Sequence Analysis of a 24kDa Protein from Luffa cylindrica Fruit Phloem Exudate | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 80 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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