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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 黃斌 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:53Z | - |
| dc.date.available | 2021-07-01T08:19:53Z | - |
| dc.date.issued | 1997 | |
| dc.identifier.citation | 林綠瑩 1989 低溫逆境對綠豆白化幼苗生長、根尖顯微構造，及細胞膜、液胞膜的影響 國立臺灣大學植物研究所碩士論文 馬淑芳 1988 低溫逆境對耐寒和不耐寒植物之外部形態、內部微細構造和蛋白質合成比較 國立臺灣大學植物研究所碩士論文 郭金龍 1991 低溫逆境下植物體氧化還原?、蔗糖還原?活性的改變 國立臺灣大學植物研究所碩士論文 郭惠芬 1996 酒精逆境對大豆幼苗的影響及胺基酸類似物誘導大豆第一族低分子量熱休克蛋白質生合成之定量分析 國立臺灣大學植物研究所碩士論文 張孟瑤 1989 低溫馴化對綠豆幼苗生理與生化改變的影響 國立臺灣大學植物研究所碩士論文 楊敏宗 1990 低溫逆境下綠豆白化幼苗之RNA Polymerase活性與蛋白質合成之探討 國立臺灣大學植物研究所碩士論文 劉炫亨 1993 綠豆幼苗經溫度調馴後在溫度逆境下形態及生理之變化 國立臺灣大學植物研究所碩士論文 Alberdi, M., and L.J. Corcuera 1991 Cold acclimation in plants. Phytochemistry 30: 3177-3184 Anderson, J.V., Q.B. Li, D.W. Haskell, and C.L. Guy 1994 Structural organization of spinach nedoplasmic reticulum-Luminal 70-kilodalton heat-shock cognate gene and expression of 70-kilodalton heat-shock genes during cold acclimation. Plant Physiol. 104:1359-1370 Boston, R.S., P.V. Viitanen, and E. Vierling 1996 Molecular chaperones and folding in plants. Plant Mol.Biol. 32:191-222 Burke, M.J., L.V. Gusta, H.A. Quamme, C.J. Weiser, and P.H. Li 1976 Freezing and injury in plants. 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Plant Physiol. 103:667-668 Yoshida, S. 1991 Chilling-induced inactivation and its recovery of tonoplast H+-ATPase in mungbean cell suspension cultures. Plant Physiol. 95:456-460 Yoshida, S. 1995 Low-temperature-induced alkalization of vacuoles in suspension-cultured cells of mungbean (Vigna radiata L. Wilczk). Plant Cell Physiol. 36: 1075-1079 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76286 | - |
| dc.description.abstract | 綠豆?不耐低溫性植物，將其白化幼苗放在低溫4℃處理12小時後，便會有延緩生長的現象；而在處理2天後，進行回溫時則會有相當明顯的傷害出現並且死亡。 將白化幼苗以40℃進行短暫熱休克前處理30分鐘，便可明顯降低其在低溫下的傷害且能夠在回溫後繼續生長，而隨著熱休克時間的增加至2小時，其在低溫逆境下可耐到長達12天以上的時間。 綠豆白化幼苗在高溫40℃處理2小時後，經35S-methionine放射性元素標定、雙向電泳的分析與熱休克蛋白質抗體反應，發現有一群高、中、低分子量熱休克蛋白質被誘導出來，而由抗體所辨識的一群19-21kD第一族低分子量熱休克蛋白質會隨熱休克時間不同而生成量不同；在致死溫度（4℃）與冷馴化溫度（10℃）處理下這群19-21kD蛋白質並不被誘導生成。此蛋白質的量在常溫（28℃）下可維持約48小時，至72小時則完全消失；在4℃低溫下可維持超過12天。除了正面證明誘導的熱休克蛋白質愈多，其幼苗在低溫下的忍受力（生長勢）愈強外，亦反面證明此低分子量熱休克蛋白質消失時，幼苗便會喪失其低溫耐性。 以抗大腸桿菌60kD與抗酵母菌60kD熱休克蛋白質抗體檢測反應，發現在綠豆幼苗處理過程中，並沒有與抗體性質相似的中分子量熱休克蛋白質被誘導出來。 藉由熱休克處理來增加低溫忍受性的研究在過去已有報導，但均未直接針對低分子量熱休克蛋白質的角色進行探討，是以本研究已確立了綠豆第一族低分子量熱休克蛋白質在低溫下的生理特性，並證明其與綠豆幼苗耐冷性間的相關性。 | zh_TW |
| dc.description.abstract | The growth of etiolated mungbean seedlings (Vigna radiata L. cv. Tainan No.5), a chilling-sensitive crop, was significantly inhibited by low temperature (4℃) treatment of 12 hr, and a significant injury appeared 2 days after low temperature treatment followed by 1 day recovery at 28℃. Pretreatment of 40℃ could improve chilling tolerance of mungbean seedlings, and a set of isotope labeled HSPs was separated by 1-D gel electrophoresis. A group of 19-21kD class I low-molecular-weight HSPs was recognized by westerrn blotting using rabbit anti-soybean class I low-molecular-weight heat-shock protein antibody. This group of 19-21kD HSPs was only induced by high temperature at 40℃ and reached the maximum level at 2 hr. The half life of these 19-21kD HSPs were about 2 days at 28℃, but the amount of proteins maintained at high level at 4℃ for 12 days. The more of the 19-21kD HSPs, the more chilling tolerance was observed. When these 19-21kD HSPs were degraded completely, the plant has lost its chilling tolerance. Using E. coli and yeast 60kD HSPs antibodies for western blot analysis, no HSPs ranging in 80kD to 40kD in mungbean seedlings were detected. The roles of each group of HSPs are still yet to be clearfied. Furthermore, the chilling tolerance observed in our mungbean system could be achieved by multiple HSPs but not only a single HSPs. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:53Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 中文摘要…………………………………………………i 英文摘要…………………………………………………ii 前 言………………………………………………………1 藥品縮寫…………………………………………………9 材料與方法………………………………………………10 結 果……………………………………………………19 討 論……………………………………………………22 圖 表……………………………………………………29 參考文獻…………………………………………………46 | |
| dc.language.iso | zh-TW | |
| dc.title | 綠豆第一族低分子量熱休克蛋白質與低溫耐性之探討 | zh_TW |
| dc.title | The Studies on Class I Low-molecular-weight Heat Shock Proteins and Chilling Tolerance in Mungbean (Vigna radiata L.) | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 59 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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