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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Pi-Shiang Lai | en |
| dc.contributor.author | 賴碧香 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:34Z | - |
| dc.date.available | 2021-07-01T08:19:34Z | - |
| dc.date.issued | 1979 | |
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Van Kammen 1978 “The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of Leghemoglobin in pea nodules induced by Rhizobium leguminosarum.” Biochim Biophys. Acta539: 1-11. 7. Chen pin-ching and Donald A. phillips 1977 “Induction of root nodule senescence by combined nitrogen in Pisum sativum L.” Plant Physiol. 59: 440-442. 8. Cheniae G. M. and H. J. Evans 1959 “Properties of a particulate nitrate reductase from the nodules of the soybean plants.” Biochim. Biophys. Acta 35: 140-153. 9. Dimiler R. J., W. C. Schaefer, C. S. Wise and C. E. Rist 1952. “Quantitative paper chromatography of D-glucose and its oligosaccharides,” Anal. Chem. 24: 1411-1414. 10. Dunn S. D. and Robert V. Klucas 1973 “Studies on possible routes of ammonium assimilation in soybean root nodule bacteroids.” Can. J. Microbiol 19: 1493-1498. 11. Evans H. J. 1954 “Diphosphoyridine nucleotide-nitrate reductase from soybean nodules.” Plant physiol. 29: 298-321. 12. Evans H. J., Burton, Koch and Robert Klucas 1972 “Prepration of nitrogenase from nodules and separation into components.” Methods Enzymol. 24B: 470-477. 13. Hageman R. H. and D. P. Huckleshy 1971 “Nitrate reductase from higher plants.” Methods Enzymol. 23: 491-503. 14. Harper J. E. and Richard L. Cooper 1971 “Nodulation response of soybeans (Glycine max. L. Merr.) to application rate and placement of combined nitrogen.” Crop Science 11: 438-440. 15. Herridge D.F. and John S. Pate 1977 “Utilization of net photosynthate for nitrogen fixation protein production in an annual legume.” Plant physiol. 60: 759-764. 16. Hewitt E. J. and M. M. R. K. Afiridi 1959 “Adaptive synthesis of nitrate reductase in higher plants.” Nature 183: 57 17. Huang C. Y., J. S. Boyer and L. N. Vanderheef. 1975 “Limitation of acetylene reduction (nitrogen fixation) by photosynthesis in soybean having low water potentials.” Plant Physiol. 36: 228-232. 18. Kennedy IR. J. Rigaud and J. C. Trichant 1977 “Nitrate reductase from bacteroids of Rhizobium japonicum enzyme characteristics and possible interaction with nitrogen fixation.” Biochim. Biophys. Acta 397: 24-35. 19. Lawn R. J. and W. A. Burn 1974 “Symbiotic nitrogen fixation in soybeans 1. effect of photosynthetic source-sink manipulation.” Crop Science 64: 11-16 20. Lowry O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall 1951. “Protein measurement with the Folin phenol reagent.” J. Biol. Chem. 192: 265-275. 21. Lyons J. C. and E. B. Earley 1952 “The effect of ammonium nitrate application to field soils on nodu1ation, seed yield and nitrogen and oil content of soybean.” Soil SCI. SOC. Amer. Pro. 16: 259-263. 22. Mcparland R. H., J. G. Guevara, R.R. Becker and H. J. Evans. 1976 “The purification and properties of the glutamine synthetase from the cytosol of soya-bean root nodules.” Biochem. J. 153: 597-606. 23. Moore Stanford and William H. Stein 1954 “A modified Ninhy-drin reagent for the photometric determination of amino acids and related compounds,” J. B. C. 211: 907-911. 24. O'Neal D. and K. W. Joy 1973 “Glutamine synthetase from pea leaves. 1. purification. stabilization and PH optima. Arch. Biophys. 159: 113-122. 25. Orcutt F. S. and P. W. Wilson 1935 “The effect of nitrate-nitrogen on the carbohydrate metabolism of inoculated soybeans.” Soil Science 39:289-295. 26. Phillips DA. KD Newell, SA Hassell. CE Felling 1976 “The effect of CO2 enrichment on root nodule development and symbiotic N2reductuin in Pisum sativum L.” Am. J. Bot. 63:356-362. 27. Rigaud J. F. J. Bergersen, G. L. Turner and R. M. Daniel 1973 “Nitrate dependent anaerobic acetylene reduction and nitrogen fixation by soybean bacteroids.” J. Gen. Microbiol. 77: 137-144. 28. Scholl R. L., J. E. Harper, and R. H. Hageman 1974 “Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate.” Plant physiol. 53: 825-828. 29. Sloger C. 1973 “Assimilation of ammonia by glutamine syntheatse in N2-fixing bacteroids from soybean nodules.” Plant physiol. 54: 34 (suppl.) 30. Tanner J. W. and I. C. Anderson 1963 “An external effect of inorganic nitrogen in root nodulation.” Nature 198: 303-304. 31. Thornton G. D. 1946 “Greenhouse studies of nitrogen fertilization of soybeans and lespedeza during isotopic nitrogen.” Soil Sci. Soc. Amer. Pro. 11: 249-251. 32. Tubb R. S. 1974 “Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella.” Nature 251: 381-384. 33. Tubb R. S. and J. R. Postgate 1973 “Control of nitrogenase synthesis in Klebsiella pneumoniae.” J. Gen. Microbiol 79: 103-117. 34. Wilson, D. O., and H. M. Reisenauer 1963 “Determination of leghemoglobin in legume nodules.” Analytical Biochemistry 6: 27-30 35. Woolfolk C. A., B. Shapiro and E. R. Stadtman 1966 “Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Esherichia coli.” Arch. Biochem Biophys. 166: 177-192. 36. 黃啟穎 1978 “高等植物固氮作用的理論與實際” 中央研究中心專刊第8號—遺傳工程與固氮作用pl-30. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76256 | - |
| dc.description.abstract | 在大豆開花時期施以100毫莫耳硝酸銨，根瘤固氮酵素活性受到抑制，抑制程度隨著處理日數的增加而增加，若以單位根瘤來計算；由第一天活性為對照組的67%，降至第三、五天為對照組的25%，14%根瘤血紅素含量也隨著處理日數的增加而減少，由第一天其含量為對照組的90％降至第三、五天為對照組的62%，30%；硝酸還原酵素活性卻隨著硝酸銨處理日數的增加而增加，此現象與固氮酵素活性因硝酸銨處理而降低成強烈對比；硝酸銨的處理對於葉片及根瘤的麩醯胺合成酵素活性沒有顯著的影響。而發現硝酸銨的處理，可增加可溶性糖的利用率；而由根瘤及葉片硝酸還原酵素活性來比較，發現後者活性約為前者的兩倍，此顯示硝酸的還原作用主要是在葉片中進行；對於固氮作用因硝酸銨處理而受抑制的原因可能是因硝酸銨的處理造成根瘤血紅素合成速率的降低或分解速率的增高，引起血紅素含量的降低，血紅素攜帶氧功能的減弱，而促使固氮酵素活性的降低。 | zh_TW |
| dc.description.abstract | The nodular nitrogenase activity is inhibited while the plants are supplied with ammonium nitrate. The ratio of nitrogenase activity of the ammonium nitrate treated plant to that of the control is declined from 67% to 14% on the fifth day after treatment. The leghemoglobin in nodule is also decreased by the ammonium nitrate. The reduction of nitrogenase activity is compatible with the decrement of Leghemoglobin content by the treatment. The ammonium assimilation enzyme, glutamine synthetase, remaines unchange, however the nitrate reductase is enchanced by the ammonium nitrate. The nitrate reductase is obviousely induced by the pressence of ammonium nitrate, because no activity of this enzyme can be detected in the control plants. The leaf is the main tissue of plant to reduce and assimilate ammonium nitrate. It has been found that nitrate reductase activity in leaf is two fold higher than that in nodule. The nitrogenase activity is antiparrelled with nitrate reductase, no synergistic effect of nitrogenase activity and nitrate reductase on nitrogen metabolism is found in this study. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:34Z (GMT). No. of bitstreams: 0 Previous issue date: 1979 | en |
| dc.description.tableofcontents | 一、中文摘要………………………………………………………………………1 二、英文摘要………………………………………………………………………2 三、前 言………………………………………………………………………3 四、實驗材料與方法………………………………………………………………7 五、結 果………………………………………………………………………19 六、討 論………………………………………………………………………37 七、參考文獻………………………………………………………………………41 | |
| dc.language.iso | zh-TW | |
| dc.title | 硝酸銨對於大豆固氮酵素，麩醯胺合成酵素及硝酸還原酵素活性以及化學組成的影響 | zh_TW |
| dc.title | The Effects of Ammoniuni Nitrateon the Activity of Nitrogenase, Glutamine Synthetase, Nitrate Reductase and Chemical Composition of Soybean | en |
| dc.date.schoolyear | 67-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 45 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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