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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林哲揚 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:33Z | - |
| dc.date.available | 2021-07-01T08:19:33Z | - |
| dc.date.issued | 1996 | |
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E. and Goodnew, M. M. 1992. C-ski cDNAs are encoded by eight exons, six of which are closely linked within the chicken genome. Nuucleic Acids Res. 20:1511-1516. Heyman, H. C. and Stavnezer, E. 1994. Carboxyl-terminal region of the Ski oncoprotein mediates homodimerization as well as heterdimerization with the related protein SnoN. J. Biol. Chem. 269:26996-27003. Hromas, R., Collins, S. J., Hickstein, D., Raskind, W., Deaven, L. L., OHara, P., Hagen, F. S. and Kaushansky, K. 1991. A Retinoic acid- responsive human zinc finger gene, MZF-I, preferential expressed in myeloid cells. J. Biol. Chem. 266:14183-14187. La Rocca, S. A., Agrossi, M., Falcone, G., Aelma, S. and Tato, F. 1989. An interaction with normal cells suppresses the transformed phenotype of v-myc-transformed quail muscle cells. Cell 15:596-603. Larsen, J., Beug, H. and Hayman, M. J. 1992. The c-ski oncogene cooperates with the v-sea oncogene in erythroid transformation by blocking erythroid differentiation. 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Nagase, T., Nomura, N. and Ishii, S. 1993. Complex formation between proteins encoded by the ski gene family. J. Biol. Chem. 268:13710- 13716. Namciu, S., Lieberman, M. A. and Stavnezer, E. 1994. Induction of the c-ski proto-oncogene by phorbol ester correlates with induction of megakaryocyte differentiation. Oncogene 9:1407-1416. Nomura, N., Sasamoto, S., Ishii, S., Date, T., Matsui, M. and Ishizaki, R. 1989. Isolation of human cDNA clones of ski and the ski-related gene,sno. Nucleic Acids Res. 17:5489-5500. Orkin, S. H. 1990. Globin gene regulation and switching: Circa 1990. Cell 63:665-672 Pearson-White, S. 1993. SnoI, A novel alternatively spliced isoform of the ski proto oncogene homolog, sno. Nucleic acids Res. 21:463-4638. Pevny, L., Simon, M. C., Robertson, E., Klein, W. H., Tsai, S-F., D’Agati, V., Orkin, S. H. and Constantini, F. 1991. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature 349:257-260. Shapiro, M. B. and Senapathy, p. 1987. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acid Res. 15:7155-7174. Sleeman, J. P. and Laskey, R. A. 1993. Xenopus c-ski contains a novel coiled-coil protein doman.and is maternally expressed during development. Oncogene 8:67-77. Stavnezer, E., Gerhard, D. S., Binari, R. C. and Blzas, I. 1981. Generation of transforming virus in cultures of chicken fibroblasts infected with an avian leukosis virus. J. Virol. 39:920-934. Stavnezer, E., Barkas, A. E., Brennan, L. A., Brodeur, D. and Li, Y. 1986. Transforming Sloan-kettering virus generated from the cloned v-ski oncogene by in vitro and in vivo recombination. J. Virol. 57:1073-1083. Sutrave, P. and Hughes, S. H. 1989. Isolation and characterization of three distinct complementary DNA species for the chicken c-ski gene. Mol. Cell. Biol. 9:4046-4051. Sutrave, P., Kelly, A. M. and Hughes, S. H. 1990a. Ski can cause selective growth of skeletal muscle in transgenic mice. Genes. Dev. 4:1462-1472. Sutrave. P., Copeland, T. D., Showalter, S. D. and Huhes, S. H. 1990b. Characterization of chicken c-ski oncogene products expressed by retrovirus vectors. Mol. Cell. Biol. 10:3137-3 144. Tao, J.-X. and Ip, W. 1991. Site specific antibodies block kinase A phosphorylation of desmin in vitro and inhibit incorporation of myoblasts into myotubes. Cell Motil. Cytoskel. 19:109-120. Thayer, M.J., Tapscott, S. J., Davis, R. L., Wright, W. E., Lassar, A. B. and Weintraub, H. 1989. Positive autoregulation of the myogenic determination gene MyoDl. Cell 58:241-248. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76253 | - |
| dc.description.abstract | 由 1.6 公分長的吳郭魚萃取 mRNA ，加入針對exon l 設計的 degenerate primer 進行 RT－PCR ，得到四種吳郭魚 c－ski cDNA exon l 的核甘酸片段。四種吳郭魚c－ski cDNA exon l 核甘酸片段之中，三種有 309 個核甘酸，一種有 300 個核甘酸。第一種到第四種吳郭魚c－ski cDNA exon l 與雞 c－ski cDNA 第 468 到第 773 核甘酸各有 87.79％、88.35％、 88.35 ％以及 88.67 ％相同。蛋白質轉譯區由第一個核甘酸到第309個及第300個核甘酸共有103個胺基酸以及100個胺基酸。第一種到第四種吳郭魚 c－ski cDNA exon 1 與雞 c－Ski 從N-端第 101 個胺基酸到第 202 個胺基酸各有 90.10％。 85.15％ 8 .15％、82.18％相同。
針對exon l 以及 exon 6 設計 degenerate primer 進行 RT 一 PCR 之後得到第一種以及第二種吳郭魚c－ski cDNA 核甘酸片段，彼此的核甘酸有 76 . 20 ％相同。蛋白質轉譯區由第 1 個核甘酸到第1290個以及1452個核甘酸一共有430個以及484 個胺基酸 ，與雞c－ski第 101 個到 599 個胺基酸有54.65％以及56.28％相同。 建立吳郭魚 genomic library ，以吳郭魚 type I c－ski cDNA exon l 核甘酸片段做為探針進行篩選。選殖出一段 genomic DNA 共14 kb ，以 Sacl 作用之後進行南方轉漬分析（ Southern blot ）。發現吳郭魚 type I c－ski exon l 位於 4 kb 的核甘酸片段上：其中 exon l 有 894 個核甘酸； 5 ' flanking region 及 5 'untranslated region 有596個核甘酸；其餘為 intron 約有2 . 5 kb 。在 5 ' flanking region 中， ATG 上游-486－502 個核甘酸有 MyoD response element ；-334－341 個核甘酸有 MZF 1 response element ； -284－293 個核甘酸有 GATA－1 response element ；而 TATA box 則位於 -229－234 個核甘酸的位置上。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:33Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . . . . . . . . . . . . 1 文獻回顧. . . . . . . . . . . . . . . . . . . 2 壹、 V—ski 與c—ski sequence . . . . . . . . . 2 貳、Ski protein . . . . . . . . . . . . . . . . . . . 5 參、 The expression of different transcripts . . . . . . 10 肆、 Relative to other oncogenes . . . . . . . . . 13 伍、 Biological activities . . . . . . . . . . . 15 緒論. . . . . . . . . . . . . . . . . . . . . . . 19 材料與方法. . . . . . . . . . . . . . . . 20 壹、材料. . . . . . . . . . . . . . . . . . . . . .20 貳、方法. . . . . . . . . . . . . . . . . . . . . .21 A. RT－PCR . . . . . . . . . . . . . . . . . . . . 21 B. genomic DNA library 之建立 . . . . . . 27 C．篩選（ screen ) . . . . . . . . . . . . . . . 31 D. Southern blot analysis . . . . . . . . . . . 35 E. cDNA library 之建立 . . . . . . . 36 結果. . . . . . . . . . . . . . . . . . . . . . . . 40 A. RT－PCR product : c—ski cDNA 片段. . . . . . . .40 B. genomic 1ibrary . . . . . . . . . . . . . . . 43 C. cDNA 1ibrary . . . . . . . . . . . . . . 45 討論. . . . . . . . . . . . . 47 A. RT－PCR . . . . . . . . . . . . . . . . . . . . 47 B. tilapia c—ski type I 之genomic DNA . . . . . . 52 sequence deduced amino acid sequence的分析 C. cDNA library screen . . . . . . . . . 55 參考文獻. . . . . . . . . . . . . . . . . . 56 圖表. . . . . . . . . . . . . . . . . . 60 附圖一 . . . . . . . . . . . . . . . . 104 二 . . . . . . . . . . . . . . . . . 105 三 . . . . . . . . . . . . . . . . . 106 附錄 . . . . . . . . . . . . . . . . 107 | |
| dc.language.iso | zh-TW | |
| dc.title | 吳郭魚c-ski基因的選殖, 定序及比較 | zh_TW |
| dc.title | Molecular cloning of tilapia(Oreochronis aurea ) c-ski gene | en |
| dc.date.schoolyear | 84-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 113 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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