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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Lim Jormay | en |
dc.contributor.author | 林若梅 | zh_TW |
dc.date.accessioned | 2021-07-01T08:19:30Z | - |
dc.date.available | 2021-07-01T08:19:30Z | - |
dc.date.issued | 1996 | |
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(1994) Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa. Proc. Natl. Acad. Sci. USA 91: 974-978 Rao, M, J, K. and Argos, P. (1986) A conformational preference parameter to predict helices in integral membrane proteins. Biochim. Biophys. Acta 869: 197-214 Reig, C., Antich, J., Gean, E., Garcia Sandoval, B., Ramos, C., Ayuso, C. and Carballo, M. (1994) Identification of a novel rhodopsin mutation (Met-44-Thr) in a simplex case of retinitis pigmentosa. Human Genetics 94: 283-286 Robinson, P. R., Buczylko, J., Ohguro, H. and Palczewski, K. (1994) Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase. Proc. Natl. Acad. Sci. USA 91: 5411-5415 Robinson, J., Schimitt, E. A., H?rosi, F. I., Reece, R. J. and Dowling, J. F. (1993) Zebrafish ultraviolet visual pigment: Absorption spectrum, sequence and localization. Proc. Natl. Acad. Sci. USA 90:6009-6012 Rodriguez, J. A., Herrera, C. A., Birch, D. G. and Daiger, S. P. (1994) A leucine to arginine amino acid substitution at codon 46 of rhodopsin is responsible for a severe form of autosomal dominant retinitis pigmentosa. Human mutaion 2: 205-213 Sakmar, T. P., Franke, R. R. and Khorana, H. G. (1989) The role of the retinylidene Schiff base counterion in rhodopsin in determining wavelength absorbance and Schiff base pKa. Proc. Natl. Acad. Sci. USA 86: 8309-8313 Sakmar, T. P., Franke, R. R. and Khorana, H. G. (1991) Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc. Natl. Acad. Sci. USA 88: 3079-3083 Sheshberadaran, H. and Takahashi, J. S. (1994) Characterization of the chicken rhodopsin promoter: Identification of retina-specific and glass-like protein binding domains. Mol. Cell. Neurocsi. 5: 309-318 Shyue, S.-K., Li, L., Chang, B. H.-J. and Li, W.-H. (1994) Intronic gene conversion in the evolution of human X-linked color vision genes. Mol. Biol. 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Cell 40: 851-858 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76246 | - |
dc.description.abstract | 為了研究視覺基因的分子結構與有關魚類生理學視覺訊息傳導的生化起始反應,首先得定出視覺基因的核酸序列.從鯉魚視網膜cDNA基因庫,第一種視紫質的cDNA (Tsai et al., 1994)與第二種視紫質部分的cDNA (Chong, 1994)已被選殖.本論文的實驗定出完整的第二種視紫質的cDNA序列並分析之.第二種視紫質的胺基酸序列顯示兩種視紫質之間有五個胺基酸相異,即(i)第一種的第十九個胺基酸是Val而第二種的是Ile (Val-19-Ile), (ii)Ile-54-Val, (iii)Ile-108-Val, (iv) Val-169-Glu,與(v)His-315-Asn.除此,第二種視紫質基因的3’端非轉譯區比第一種的長99鹼基,並且含四個polyadeny1ation signal而不是一個.再者,本人建立了一個genomic基因庫並從裡面分離出第二種視紫質的genomic DNA.此genomic DNA被定序及其轉錄之起始位置(transcription start site)也經引子延長化驗(primer extention assay)而被確定.實驗結果顯示,(1)此基因序列沒有內子(intron),(2)其轉錄起始位置其他脊椎動物相同,及(3)在其上游序列含有保守區域(conserved region)和視網膜專一性核蛋白結合位置(retina-specific nuclear protein binding sites).而南方分析(Southern analysis)與反轉錄聚合酵素鍊鎖反應(reverse transcriptase-polymerase chain reaction)表示兩種視紫質乃存在於同一個鯉魚群但是不同的個體裡.另一方面,利用綠光視覺接受器(green opsin)的cDNA當做探針,本人從genomic基因庫篩選出一個類似retinal epithelium-specific protein的克隆(clone).結果其中一片段的序列與人類衛星去氧核醣核酸(human satellite DNA)有66%相似而另一段則與thromboxane synthase有95%相似.但此克隆的真正身份卻有待進一步的研究. | zh_TW |
dc.description.abstract | To investigate the molecular structures of the visual pigment genes and the initial biochemical reactions involved in the visual signal transduction relevant to fish physiology, the sequence of the visual pigment gene has to be determined. A putative cDNA encoding carp type I rhodopsin (Tsai et al., 1994) and a partial cDNA encoding type II rhodopsin (Chong, 1994) had been cloned from a retinal cDNA library. In the present work, the cDNA of the type II rhodopsin was completely sequenced and analyzed. The deduced amino acid sequence of type II rhodopsin reveals that there are only five amino acid residues differences between type I and type II, i.e.. (i) the 19th residue in type I is Val, instead of Tie in type II (Val-19-Ile), (ii) Ile-54-Val, (iii) Ile-108-Val, (iv) Val-169-Glu, and (v) His-315-Asn. Besides, there are 4 polyadenylation signals in type II rhodopsin instead of I in type I, and the 3’ untranslated region of type II is 99 bases longer. Furthermore, a genomic library of the common carp was constructed and the genomic DNA of type II rhodopsin was isolated. The genomic DNA was sequenced and the primer extension assay was performed to determine the transcription start site. The results reveal that (1) the gene is not interrupted by any intron, (2) the initiation of the transcripts of rhodopsin II is similar to that of the other vertebrates, and (3) there are conserved regions and retina-specific nuclear protein binding sites found in the upstream region. The Southern analysis of genomic DNA and the reverse transcriptase-polymerase chain reaction imply that the two types of rhodopsins are from different individuals of the same population. On the other hand, a clone (3aV1) which resembles the retinal epithelium-specific protein gene was screened from the genomic DNA library using the partial cDNA of green opsin as a probe. The results indicate that a fragment of the deletion clones of 3aV1 is 66% identical to the human satellite DNA and another fragment is 95% identical to the thromboxane synthase. However, the real identity of the clone remains to be investigated. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:30Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
dc.description.tableofcontents | Abstract…………………………………………1 Literature review…………………………………3 Background……………………………………3 1. Maximal wavelength of spectral absorption…3 2. Signal transduction mechanism………………4 3. Structure and function relationship…………4 4. Naturally occurred mutation: retinitis pigmentosa…5 5. The genes encoding visual pigment …………6 Chapter 1 cDNA and deduced amino acid sequence of type II rhodopsin…………………………………………8 1.1 Introduction…………………………………8 1.2 Materials and methods………………………8 1.3 Results and Discussion………………………9 1.3.1 Primary structure of putative type II rhodopsin gene…9 1.3.2 Secondary structure of carp rhodopsin………………10 Chapter 2 Molecular structure of type II rhodopsin gene………12 2.1 Introduction…………………………………12 2.2 Materials and methods………………………13 2.2.1 Construction of genomic library……………13 2.2.2 Screening ……………………………………15 2.2.3 Southern blotting ……………………………18 2.2.4 Subcloning …………………………………18 2.2.5 Deletion construct …………………………19 2.2.6 Sequencing …………………………………19 2.2.7 Extraction of total RNA ……………………21 2.2.8 Primer extension assay ………………………21 2.2.9 Northern blotting ……………………………22 2.2.10 Southern analysis of genomic DNA………22 2.2.11 Reverse transcriptase-polymerase chain Reaction………………23 2.3 Results and Discussion……………………………25 2.3.1 Isolation of the rhodopsin gene………………25 2.3.2 Deletion clones………………………………25 2.3.3 Coding region…………………………………25 2.3.4 Primer extension assay………………………26 2.3.5 Northern blotting analysis……………………26 2.3.6 Upstream consensus sequence…………………27 2.3.7 The existence of the types is individual dependent……………28 Chapter 3 Clone resembles retinal epithelium-specific protein gene……………………………………………29 3.1 Introduction…………………………………………29 3.2 Materials and methods ………………………………29 3.3 Results and Discussion ………………………………30 3.3.1 Isolation of the clone with positive signal………30 3.3.2 Sequence analysis……………………………30 References……………………………………………31 Figures………………………………………………36 Table…………………………………………………67 Appendix………………………………………………68 | |
dc.language.iso | zh-TW | |
dc.title | 鯉魚視網膜紫質基因之分子結構 | zh_TW |
dc.title | Molecular Structure of Carp Visual Pigment Genes | en |
dc.date.schoolyear | 84-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 77 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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