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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Lim Jormay | en |
| dc.contributor.author | 林若梅 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:30Z | - |
| dc.date.available | 2021-07-01T08:19:30Z | - |
| dc.date.issued | 1996 | |
| dc.identifier.citation | Ahmad, I. (1995) Mash-1 is expressed during rod photoreceptor differentiation and binds an E-box, Eopsin-1 in the rat opsin gene. Developmental Brain Res. 90: 184-189 Ahmad, I., Yu, X. and Barnstable, C. J. (1994) A cis-element, Tα-1, in the upstream region of rod α-transducin gene that binds a developmentally regulated retina-specific nuclear factor. J. Neurochem. 62: 396-399 Al Magtheh, M., Inglehearn, C., Lunt, P., Jay, M., Bird, A. and Bhattacharya, S. (1994) Two new rhodopsin transversion mutations (L40R; M216K) in families with autosomal dominant retinitis pigmentosa. Human mutaion 3:409-410 Al-Ubaidi, M. R., Pittler. S. J., Champagne, M. S., Triantafyllos, J. T., McGinnis, J. M. and Baehr, W. (1990) Mouse opsin: Gene structure and molecular basis of multiple transcripts. J. Biol. Chem. 265: 20563-20569 Alexander, G.. Sweeting, R. and McKeown, B. (1994) The shift in visual pigment dominance in the retinae of juvinile coho salmon (Oncorhynchus kisutch): An indicator of smolt status. J. Exp. Biol. 195: 185-197 Arsenjo, A. B., Rim, J. and Oprian, D. D. (1994) Molecular determinants of human red/green color discrimiNatlon. Neuron 12: 1131-1138 Bowmaker. J. K., Govardovskii, V. I., Shukolyukov, S. A., Zueva, L. V., Hunt, D. M., Sideleva, V. G. and Smirnova, O. G. (1994) Visual pigments and the photic environment: the cottoid fish of Lake Baikal. Vision Res. 34:591-605 Bowmaker, J. K. and Kunz, Y. M. (1987) Ultraviolet receptors, tetrachromatic color vision and retinal mosaics in the brown trout (Salmo trutta): Age-dependent changes. Vision Res. 27(12): 2101-2108 Buchman, A. R. and Berg, P. (1988) Comparison of intron-dependent and intron independent gene expression. Mol. Cell. Biol. 8: 4395-4405 Chan, T., Lee, M. and Sakmar, T. P. (1992) Introduction of hydroxyl-bearing amino acids causes bathochromic spectral shifts in rhodopsin. J. Biol. Chem. 267: 9478-9480 Chong, J.-L. (1994) Some Preliminary Characteristics of the photoreceptor genes from a carp retina cDNA library. Master thesis, Natlonal Taiwan University, Taipei, Taiwan. Davidson, F. F., Loewen, P. C. and Khorana, H. G. (1994) Structure and function in rhodopsin: Replacement by alanine of cysteine residues 110 and 187, components of a conserved disulfide bond in rhodopsin, affects the light-activated metarhodopsin II state. Proc. Natl. Acad. Sci. USA 91: 4029-4033 Doi, T., Molday, R. S. and Khorana, H. G. (1990) Role of the intradiscal domain in rhodopsin assembly and function. Proc. Natl. Acad. Sci. USA 87: 4991-4995 Downing, J. E. G., Djamgoz, M. B. A. and Bowmaker, J. K. (1986) Photoreceptors of cyprinid fish, the roach: morphological and spectral characteristics. J. Comp. Physiol. A 159: 859-868 Fitzgibbon, J., Hope, A., Slobodyanyuk, S. J., Bellingham, J., Bowmaker, J. K. and Hunt, D. M. (1995) The rhodopsin-encoding gene of bony fish lacks introns. Genes 64:273-277 Franke, R. R., Konig, B., Sakmar, T. P., Khorana, H. G. and Hofmann K. P. (1990) Rhodopsin mutants that bind but fail to activate transducin. Science 250: 123-125 Fukada, M. N., Papermaster, D. S. and Hargrave, P. A. (1979) Rhodopsin carbohydrate: Structure of small oligosaccharides attached at two sites near the NH2 terminus. J. Biol. Chem. 254:8201-8207 Ginkel, P. R. and Hauswirth, W. W. (1994) Parallel regulation of fetal gene expression in different photoreceptor cell types. J. Biol. Chem. 269: 4986-4992 Govardhan, C. P. and Oprian, D. D. (1994) Active site-directed inactivation of constitutively active mutants of rhodopsin. J. Biol. Chem. 269: 6524-6527 H?rosi, F. I. and Hashimoto, Y. (1983) Ultraviolet visual pigment in a vertebrate: A tetrachromatic cone system in the Dace. Science 222:1021-1023 Hisatomi, O., Iwasa, T., Tokunaga, F. and Yasui, A. (1991) Isolation and characterization of lamprey rhodopsin cDNA. Biochem. Biophys. Res. Comm. 174: 1125-1132 Jacobson, S. G., Kemp, C. M., Cideciyan, A. V., Macke, J. P., Sung, C.-H. and Nathans, J. (1994) Phenotypees of stop codon and splice site rhodopsin mutations causing retinitis pigmentosa. Invest. Ophthalmol. Vis. Sci. 35:2521-2534 Johnson, R. L., Grant, K. B., Zankel, T. C., Boehm, M. F., Merbs, S. L., Nathans, J. and Nakanishi, K. (1993) Cloning and expression of goldfish opsin sequences. Biochem. 32: 208-214 Konig, B., Arendt, A., Mcdowell, J. H., Kahlert, M., Hargrave, P. A. and Hofmann, K. P. (1989) Three cytoplasmic loops of rhodopsin interact with transducin. Proc. Natl. Acad. Sci. USA 86: 6878-6882 Karnik, S. S., Sakmar, T. P., Chen, H.-B. and Khorana, H. G. (1988) Cystein residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. Proc. Natl. Acad. Sci. USA 85: 8459-8463 Karnik, S. S. and Khorana, H. G. (1990) Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187. J. Biol. Chem. 265: 17520-17524 Kaushal, S. and Khorana, H. G. (1994) Structure and function in rhodopsin. 7. Point mutations associated with autosomal dominant retinitis pigmentosa. Biochem. 33: 6121-6128 Khorana, H. G. (1992) Rhodopsin, photoreceptor of the rod cell. J. Biol. Chem. 267: 1-4 Korenbrot, I J. and Fernald, R. D. (1989) Circadian rhythm and light regulate opsin mRNA in rod photoreceptors. Nature 337: 454-457 Krupnick, J. G., Gurevich, V. V., Schepers, T., Hamm, H. E. and Benovic, J. L. (1994) Arrestin-rhodopsin interaction. J. Biol. Chem. 269: 3226-3232 La Vail, M. M., Rapaport, D. H. and Rakic, P. (1991) Cytogenesis in the monkey retina. J. Comp. Neurol. 309: 86-114 Morabito, M. A., Yu, X. and Barnstable, C. J. (1991) Characterization of developmentally regulated and retina-specific nuclear protein binding to a site in the upstream region of the rat opsin gene. J. Biol. Chem. 266: 9967-9972 Morris, A., Bowmaker, J. K. and Hunt, D. M. (1993) The molecular basis of a spectral shift in the rhodopsins of two species of squid from different photic environment. Proc. R. Soc. Lond. B 254: 233-240 Naash, M. I., Hollyfield, J. G., Al Ubaidi, M. R. and Baehr, W. (1993) Simulation of human autosomal dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene. Proc. Natl. Acad. Sci. USA 90: 5499-5503 Nathans, J. (1990) Determinants of visual pigment absorbance: Identification of the retinylidene Schiff’s base counterion in bovine rhodopsin. Biochem. 29: 9746-9752 Nathans, J and Hogness, D. S. (1983) Isolation, sequence analysis, and intronexon arrangement of the gene encoding bovine rhodopsin. Cell 34: 807-814 Nathans, J and Hogness, D. S. (1984) Isolation and nucleotide sequence analysis of the gene encoding human rhodopsin. Proc. Natl. Acad. USA 81: 4851-4855 Nathans, J., Thomas, D. and Hogness, D. S. (1986) Molecular genetics of human color vision: The genes encoding blue, green, and red pigments. Science 232: 193-202 Nathans, J., Piantanida, T. P., Eddy, R. L., Shows, T. B. and Hogness, D. S. (1986) Molecular genetics of inherited variation in human color vision. Science 232: 203-232 Neitz, M., Neitz, J. and Jacobs, G. H. (1991) Spectral tuning of pigments underlying red-green color vision. Science 232: 193-202 Okano, T., Kojima, D., Fukada, Y., Shichida, Y. and Yoshizawa, T. (1992) Primary structures of chicken cone visual pigments: vertebrate rhodopsins have evolved out of cone visual pigments. Proc. Natl. Acad. Sci. USA 89:5932-5936 Palczewski, K., Mcdowell, J. H. and Hargrave, P. A. (1988) Purification and characterization of rhodopsin kinase. J. Biol. Chem. 263:14067-14073 Pittler, S. J., Fliesler, S. J. and Baehr, W. (1992) Primary structure of frog rhodopsin. FEBS 313: 103-108 Portera Cailliau, C., Sung, C.-H., Nathans, J. and Adler, R. (1994) Apoptotic photoreceptor cell death in mouse models of retinitis pigmentosa. Proc. Natl. Acad. Sci. USA 91: 974-978 Rao, M, J, K. and Argos, P. (1986) A conformational preference parameter to predict helices in integral membrane proteins. Biochim. Biophys. Acta 869: 197-214 Reig, C., Antich, J., Gean, E., Garcia Sandoval, B., Ramos, C., Ayuso, C. and Carballo, M. (1994) Identification of a novel rhodopsin mutation (Met-44-Thr) in a simplex case of retinitis pigmentosa. Human Genetics 94: 283-286 Robinson, P. R., Buczylko, J., Ohguro, H. and Palczewski, K. (1994) Opsins with mutations at the site of chromophore attachment constitutively activate transducin but are not phosphorylated by rhodopsin kinase. Proc. Natl. Acad. Sci. USA 91: 5411-5415 Robinson, J., Schimitt, E. A., H?rosi, F. I., Reece, R. J. and Dowling, J. F. (1993) Zebrafish ultraviolet visual pigment: Absorption spectrum, sequence and localization. Proc. Natl. Acad. Sci. USA 90:6009-6012 Rodriguez, J. A., Herrera, C. A., Birch, D. G. and Daiger, S. P. (1994) A leucine to arginine amino acid substitution at codon 46 of rhodopsin is responsible for a severe form of autosomal dominant retinitis pigmentosa. Human mutaion 2: 205-213 Sakmar, T. P., Franke, R. R. and Khorana, H. G. (1989) The role of the retinylidene Schiff base counterion in rhodopsin in determining wavelength absorbance and Schiff base pKa. Proc. Natl. Acad. Sci. USA 86: 8309-8313 Sakmar, T. P., Franke, R. R. and Khorana, H. G. (1991) Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin. Proc. Natl. Acad. Sci. USA 88: 3079-3083 Sheshberadaran, H. and Takahashi, J. S. (1994) Characterization of the chicken rhodopsin promoter: Identification of retina-specific and glass-like protein binding domains. Mol. Cell. Neurocsi. 5: 309-318 Shyue, S.-K., Li, L., Chang, B. H.-J. and Li, W.-H. (1994) Intronic gene conversion in the evolution of human X-linked color vision genes. Mol. Biol. Evol. 11:548-551 Sullivan. L. J., Makris, G. S., Dickinson, P., Mulhall, L. E. M., Forrest, S., Cotton, R. G. H. and Loughnan, M. S. (1993) A new codon 15 rhodopsin gene mutation in autosomal dominant retinitis pigmentosa is associated with sectorial disease. Achiv. Ophthalm. 111: 1512-1517 Sung, C.-H., Davenport, C, M. and Nathans, J. (1993) Rhodopsin mutations responsible for autosonal dominant retinitis pigmentosa: Clustering of functional classes along the polypeptide chain. J. Biol. Chem. 268: 26645-26649 Takao, M., Yasui, A. and Tokunaga, F. (1988) Isolation and sequence determination of the chicken rhodopsin gene. Vision Res. 28: 471-480 Timmers, A. M., Wintjes, E. T. and Hauswirth, W. W. (1995) Fetal topography of bovine rhodopsin mRNA suggests retinotopo-graphically determined gene expression. Invest. Ophthalmol. Vis. Sci. 36: 2008-2019 Treisman, J. E., Morabito, M. A. and Barnstable, C. J. (1988) Opsin expression in the rat retina is developmentally regulated by transcriptional activation. Mol. Cell. Biol. 8: 1570-1579 Tsai, H.-J., Shih, S.-R., Kuo, C.-M. and Li, L.-K. (1994) Molecular cloning of the common carp (Cyprinus carpio) rhodopsin cDNA. Comp. Biochem. Physiol. 109B: 81-88 Wald, G. (1965) Visual excitation and blood clotting. Science 150: 1028-1030 Wang, Y., Macke, J. P., Merbs, S. L., Zack, D. J., Klaunberg, B., Bennett, J., Gearhart, J. and Nathans, J. (1992) A locus control region adjacent to the human red and green visual pigment genes. Neuron 9: 429-440 Wang, S.-Z., Adler, R. and Nathans, J. (1992) A visual pigment from chicken that resembles rhodopsin: Amino acid sequence, gene structure, and functional expression. Biochem. 31: 3309-3315 Weitz, C. J. and Nathans, J. (1992) Neuron 8: 465-472 Yokoyama, S. (1995) Amino acid replacements and wavelength absorption of visual pigments in vertebrates. Mol. Biol. Evol. 12: 53-61 Yokoyama, R. and Yokoyama, S. (1990) Convergent evolution of the red- and green-like visual pigment genes in fish, Astyanax facsiatus, and human. Proc. Natl. Acad. Sci. USA 87: 9315-9318 Yokoyama, R. and Yokoyama, S. (1990) Isolation, DNA sequence and evolution of a color visual pigment gene of the blind cave fish Astyanax fasciatus. Vision Res. 30: 807-816 Yokoyama, R. and Yokoyama, S. (1993) Molecular characterization of a blue visual pigment gene in the fish Astyanax fasciatus. FEBS 334: 27-31 Yu, X. and Barnstable, C. J. (1994) Characterization and regulation of the protein binding to a cis-acting element, RET-1, in the rat opsin promoter. J. Mol. Neurosci. 5: 259-271 Zack, D. J., Bennett, J., Wang, Y., Davenport, C., Klaunberg, B., Gearhart, J. and Nathan, J. (1991) Unusual topography of bovine rhodopsin promoter-lacZ fusion gene expression in transgenic mouse retinas. Neuron 6: 187-199 Zuker, C. S., Cowman, A. F. and Rubin, G. M. (1985) Isolation and structure of a rhodopsin gene from D. melanogaster. Cell 40: 851-858 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76246 | - |
| dc.description.abstract | 為了研究視覺基因的分子結構與有關魚類生理學視覺訊息傳導的生化起始反應，首先得定出視覺基因的核酸序列．從鯉魚視網膜cDNA基因庫，第一種視紫質的cDNA (Tsai et al., 1994)與第二種視紫質部分的cDNA (Chong, 1994)已被選殖．本論文的實驗定出完整的第二種視紫質的cDNA序列並分析之．第二種視紫質的胺基酸序列顯示兩種視紫質之間有五個胺基酸相異，即(i)第一種的第十九個胺基酸是Val而第二種的是Ile (Val-19-Ile), (ii)Ile-54-Val, (iii)Ile-108-Val, (iv) Val-169-Glu，與(v)His-315-Asn．除此，第二種視紫質基因的3’端非轉譯區比第一種的長99鹼基，並且含四個polyadeny1ation signal而不是一個．再者，本人建立了一個genomic基因庫並從裡面分離出第二種視紫質的genomic DNA．此genomic DNA被定序及其轉錄之起始位置(transcription start site)也經引子延長化驗(primer extention assay)而被確定．實驗結果顯示，(1)此基因序列沒有內子(intron),(2)其轉錄起始位置其他脊椎動物相同，及(3)在其上游序列含有保守區域(conserved region)和視網膜專一性核蛋白結合位置(retina-specific nuclear protein binding sites)．而南方分析(Southern analysis)與反轉錄聚合酵素鍊鎖反應(reverse transcriptase-polymerase chain reaction)表示兩種視紫質乃存在於同一個鯉魚群但是不同的個體裡．另一方面，利用綠光視覺接受器(green opsin)的cDNA當做探針，本人從genomic基因庫篩選出一個類似retinal epithelium-specific protein的克隆(clone)．結果其中一片段的序列與人類衛星去氧核醣核酸(human satellite DNA)有66％相似而另一段則與thromboxane synthase有95％相似．但此克隆的真正身份卻有待進一步的研究． | zh_TW |
| dc.description.abstract | To investigate the molecular structures of the visual pigment genes and the initial biochemical reactions involved in the visual signal transduction relevant to fish physiology, the sequence of the visual pigment gene has to be determined. A putative cDNA encoding carp type I rhodopsin (Tsai et al., 1994) and a partial cDNA encoding type II rhodopsin (Chong, 1994) had been cloned from a retinal cDNA library. In the present work, the cDNA of the type II rhodopsin was completely sequenced and analyzed. The deduced amino acid sequence of type II rhodopsin reveals that there are only five amino acid residues differences between type I and type II, i.e.. (i) the 19th residue in type I is Val, instead of Tie in type II (Val-19-Ile), (ii) Ile-54-Val, (iii) Ile-108-Val, (iv) Val-169-Glu, and (v) His-315-Asn. Besides, there are 4 polyadenylation signals in type II rhodopsin instead of I in type I, and the 3’ untranslated region of type II is 99 bases longer. Furthermore, a genomic library of the common carp was constructed and the genomic DNA of type II rhodopsin was isolated. The genomic DNA was sequenced and the primer extension assay was performed to determine the transcription start site. The results reveal that (1) the gene is not interrupted by any intron, (2) the initiation of the transcripts of rhodopsin II is similar to that of the other vertebrates, and (3) there are conserved regions and retina-specific nuclear protein binding sites found in the upstream region. The Southern analysis of genomic DNA and the reverse transcriptase-polymerase chain reaction imply that the two types of rhodopsins are from different individuals of the same population. On the other hand, a clone (3aV1) which resembles the retinal epithelium-specific protein gene was screened from the genomic DNA library using the partial cDNA of green opsin as a probe. The results indicate that a fragment of the deletion clones of 3aV1 is 66% identical to the human satellite DNA and another fragment is 95% identical to the thromboxane synthase. However, the real identity of the clone remains to be investigated. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:30Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
| dc.description.tableofcontents | Abstract…………………………………………1 Literature review…………………………………3 Background……………………………………3 1. Maximal wavelength of spectral absorption…3 2. Signal transduction mechanism………………4 3. Structure and function relationship…………4 4. Naturally occurred mutation: retinitis pigmentosa…5 5. The genes encoding visual pigment …………6 Chapter 1 cDNA and deduced amino acid sequence of type II rhodopsin…………………………………………8 1.1 Introduction…………………………………8 1.2 Materials and methods………………………8 1.3 Results and Discussion………………………9 1.3.1 Primary structure of putative type II rhodopsin gene…9 1.3.2 Secondary structure of carp rhodopsin………………10 Chapter 2 Molecular structure of type II rhodopsin gene………12 2.1 Introduction…………………………………12 2.2 Materials and methods………………………13 2.2.1 Construction of genomic library……………13 2.2.2 Screening ……………………………………15 2.2.3 Southern blotting ……………………………18 2.2.4 Subcloning …………………………………18 2.2.5 Deletion construct …………………………19 2.2.6 Sequencing …………………………………19 2.2.7 Extraction of total RNA ……………………21 2.2.8 Primer extension assay ………………………21 2.2.9 Northern blotting ……………………………22 2.2.10 Southern analysis of genomic DNA………22 2.2.11 Reverse transcriptase-polymerase chain Reaction………………23 2.3 Results and Discussion……………………………25 2.3.1 Isolation of the rhodopsin gene………………25 2.3.2 Deletion clones………………………………25 2.3.3 Coding region…………………………………25 2.3.4 Primer extension assay………………………26 2.3.5 Northern blotting analysis……………………26 2.3.6 Upstream consensus sequence…………………27 2.3.7 The existence of the types is individual dependent……………28 Chapter 3 Clone resembles retinal epithelium-specific protein gene……………………………………………29 3.1 Introduction…………………………………………29 3.2 Materials and methods ………………………………29 3.3 Results and Discussion ………………………………30 3.3.1 Isolation of the clone with positive signal………30 3.3.2 Sequence analysis……………………………30 References……………………………………………31 Figures………………………………………………36 Table…………………………………………………67 Appendix………………………………………………68 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯉魚視網膜紫質基因之分子結構 | zh_TW |
| dc.title | Molecular Structure of Carp Visual Pigment Genes | en |
| dc.date.schoolyear | 84-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 77 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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