請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76242
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 徐慧雯 | zh_TW |
dc.date.accessioned | 2021-07-01T08:19:28Z | - |
dc.date.available | 2021-07-01T08:19:28Z | - |
dc.date.issued | 1996 | |
dc.identifier.citation | Andrews, C. H. and Pereira, H. G. 1967. Viruses of vertebrates. Williams and Wilkins Co., Baltimer. Bertrand, E., Pictet, R. and Grenge, T. 1993. Can hammerhead ribozyme be efficient tools to inactivate gene function Nucleic Acids Res. 22(3):293-300. Berzal-Herranz, A., Joseph, S. and Burke, J. M. 1992 In vitro selection of active hairpin ribozyme by sequential RNA-Catalyzed cleavage and ligation. Gene Dev. 6(1):129-34. Buzayan, J. M., Hampel, A. and Bruening, G. 1986. Nucleotide sequence and newly fromed phosphodiester bond of spontaneously ligated satellite tobacco ringspot virus RNA. Nucleic Acids Res. 14(24):9729-43. Collins, R. A. and Saville, B. J. 1990 A site-specific self-cleavage reaction performed by a novel RNA in Neurospora mitochondria. Cell 61:(4):685-96. Dahm, S. C. and Uhlenbeck, O. C. 1991. Role of divalent metal ions in the hammerhead RNA cleavage reaction .Biochemistry 30(39):9464-9. Denman, K. and Miller, D. C. 1993. Novel cleavage of a Hammerhead ribozyme targeted to beta-amyloid peptide precursor mRNA. Arch. Biochem. Biophys. 305(2):392-400. Dobos, P. 1993. In Vitro guanylylation of IPNV polypeptide VP1. Virology. 193:403-413. Dobos, P. 1977. Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus. J. Virol. 21:242-258. Dobos, P. and Rowe, ft 1977. Peptide map comparison of infectious pancreatic necrosis virus-specific polypeptides. J. Virol. 24:805-820. Dobos, P., Hallett, R., Kells, D. T. C., Sorensen, O. and Dowe, P. 1977. Biophysical studies of infectious pancreatic necrosis virus. J. Virol. 22:150-159. Dobos, P., Hill, B. J., Hallett, R., Kells, D. T. C., Becht, H. and Teninges, D. 1979. Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J. Virol. 32:593-605. Duncan, R., Nagy, E., Krell, D. J. and Dobos, P. 1987. Synthesis of infectious pancreatic necrosis virus polyprotein dection of a virus-encoded protease, and fine structure mapping of genome segment A coding region. J. Virol. 61: 3655-3664. Ducan, R., Mason, C. L., Nagy, E., Leong, J. A. and Dobos, P. 1991. Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encode VP1 protein: A putative RNA dependent RNA polymerase lacking the Gly-Asp-Asp Motif. Virolgy 181:541-552. Estay, A. Farias, G. Soler, M. and Kuznar, J. 1990. Further analysis on the structural proteins of IPNV. Virus research 15:85-96. Epstein, L. M. and Gall, J. G. 1987 Self-cleavage transcripts of satellite DNA from the newt. Cell 48(3):535-43. Fedor, M. J. and Uhlenbeck, O. C. 1990. Substrate sequence effect on “hammerhead” RNA catalytic efficiency. Proc. Natl. Acad. Sci. USA 87(5):1668-72. Forster, A. C., Davies, C., Hutchins, C. J. and Symons, R. H. 1990. Characterization of self-cleavage of viroid and virusoid RNAs. 181:583-607. Forster, A. C. and Symons, R. H. 1987. Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site. Cell 50(l):9-16. Forster, A. C. Jeffries, A. C. Sheldon, C. C. and Symons, R. H. 1987. Structural and ionic requirements for self-cleavage of virusoid RNAs. and trans self-cleavage of viroid RNA. Cold Spring Harbsymp Quant. Biol. 52:249-59. Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N. and Altman, S. 1983. The RNA moiety of ribonuclease P is the catalytic subunit. Cell 35:849-57. Haseloff, J. and Gerlach, W. L. 1988. Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 338: 150-153. Hsu, Y. L., Chen, B. S., and Wu, J. L. 1989(a). Comparison of RNAs and polypeptides of infectious pancreatic necrosis virus from eel and rainbow trout. J. Gen. Virol. 70:2233-2239. Hsu, Y. L., Chen, B. S., and Wu, J. L. 1989(b). The incidence infectious pancreatic necrosis virus from eel and rainbow trout in 1987. J. Fish. Dis. Inoue, T., Sullivan, F.X. and Cech, T. R. 1986. New reactions of the ribosomal RNA precursor of Tetrahymena and mechanism of self-splicing. J. Mol. Biol. 189(1):143-65. Jaeger, J. A., Turner, D. H. and Zyker, M. 1990. Predicting optimal and suboptimal secondary structure for RNA. Methods Enzymol. 183: 281-306. Kelly, R. K. and Loh, P. C. 1972. Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus. J. Virol. 10:824-834. Kruger, K., Grabowski, P.J., Zaug, A. J. , Gottschling, D. E. and Cech, T. R. 1982 Self-splicing RNA: autoexcision and autocyclization of the rRNA genes of Tetrahymena thermophila. Cell 31(1):147-57. Lai, Y. C., Lee, J. Y., Liu, H. J., Lin, J. Y. and Wu, H. N. 1996 Effects of circular Permutation on the cis-cleavage reaction of hepatitis delta virus ribozyme: application to trans-acting ribozyme design. Biochemistry 35:124-131. MacDonald, R. D. and Gower, D. A. 1981. Genomic and phenotypic divergence among three serotypes of aquatic birnaviruses (infectious pancreatic necrosis virus) of trout. Virology. 114(1):187-195. MacDonald, R. D. and Yamamoto, T. 1977. The structure of infectious pancreatic necrosis virus RNA. J. Gene. Virol. 34:235-247. Malsberger, R. G. and Cerini, C. P. 1963. Characteristics of infectious pancreatic necrosis virus. J. Bacteriol. 86: 1283-1287. Milligan, J. F., Groebe, D. R., Witherell, G. W. and Uhienbeck, O. C. 1987. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15(21):8783-98. Mizuno, T., Chou, M. Y. and Inouye, M. 1984. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcrip (micRNA). Proc. Natl. Acad. Sci. USA 81:1966-1970. M’Gonigle, R. H. 1940. Acute catarrheal enteritis of salmonid fingerlings. Trans. Am. Fish Soc. 70:297-303. Moss, L. H. and M. Grvell. 1969. Ultrastructure and sequential Development of infectious pancreatic nicrosis virus. J. Virol. 3:52-58. Nagy, E., Duncan, R., Krell, P. and Dobos, P. 1987. Mapping of the large RNA genome segment of infectious pancreatic necrosis virus by hybrid arrested translation. Virolgy 158:211-217. Perreault, J. P., Labuda, D., Usman, N., Yang, J. H. and Cedergren, R. 1991. Relationship between 2’-hydroxyls and magnesium binding in the hammerhead RNA domain: a model for ribozyme catalysis. Biochemistry 30(16):4020-5. Perriman, D., Delves, A. and Gerlach, W.L. 1992 Extended target-site specificity for a hammerhead ribozyme. Gene 113(2):157-63. Rossi, J, J. and Sarver, N. 1990. RNA enzyme (ribozyme) as antiviral therapentic agents. Trends Biotechnol. 817:179-183. Ruffner, D. E., Stormo, G. D. and Uhlenbeck, O. C. 1990. Sequence rrequirements of the hammerhead RNA self-cleavage reaction. Biochemistry 29(47): 10695-702. Sharmeen, C., Kuo, M. Y., Dinter-Gottlieb, G. and Taylor, J. 1988. Antigenomic RNA of human hepatitis delta virus can undergo self-cleavage. J. Virol. 62(8):2674-9. Symons, R. H., Hutchins, C. J., Forster, A.C., Rathjen, P. D., Keese, P. and Visvader, J. E. 1987. Self-cleavage of RNA in the replication of viroids and virusoids. J. Cell Sci. Suppl. 7:303-18. Symons, R. H. 1992. Small catalytic RNAs. Annu. Rev. Biochem. 61: 641-71. Uhlenbeck, O. C. 1987 A small catalytic oligoribonucleotide. Nature 328:596-600. Ventura, M., Wang, P. Ragot, T., Perriaudet, M. and Saragosti, S. 1993. Activation of HIV-specific ribozyme activity by self- cleavage. Nucleic Acids Res. 11:21(14):3249-3255. Wood, E. M. Snieszkom, S. F. and Yasutake, W. T. 1955. Infectious Pancreatic necrosis Virus. A. M. A. Arch. Path. 60: 26-28. Wolf, K. and Quimby, M. C. 1971. Salmonid viruses: infectious pancreatic necrosis virus, pathology and serology of first European isolations. Arch. Gesamte. Virus for sch.; 34(2):144-56. Wolf, K. and Mann, J. A. 1980. Pokilotherm Vertebrate Cell lines and virus: a current listiong for Fish. Virology 16:168-179. Wolf, K. 1966. The fish viruses. Advan. Virus Res. 12:35. Wolf, K., Sniesko, S. F., Dumbar, C. E. and Pyle, E. 1960. Virus nature of IPN in trout. Pro. Soc. Exp. Biol. Med. 104:105-108. Wu, H. N., Lin, Y. J., Lin, F. P., Makino, S., Chang, M. F. and Lai, M. M. 1989. Humam hepatitis delta virus RNA subfragments Contain an autocleavage activity. Proc. Natl. Acad. Sci. USA. 86(6):1831-5. Stram, Y. and Molad, T. 1994. Cleavage of transcripts of Foot and Mouth Disease Virus (FMDV) asial serotype, by ribozymes targeted to the VP3 and VP4 genes. Virus Gene. 9:(2)155-159. Zaug, A. J. and Cech, T. R. 1986. The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphatase. Biochemistry 25(16):447 8-82. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76242 | - |
dc.description.abstract | 感染性胰臟壞死病毒(Infectious Pancreatic Necrosis Virus; IPNV)是一種高度傳染性的病毒,在臺灣感染性胰臟壞死病毒,造成吳郭魚、鰻魚、虱目魚及文蛤等嚴重的損失。因此如何有效的防禦感染性胰臟壞死病毒是當前水產養殖界的重要課題之一。在本篇論文中,設計出具有高度專一性的鎚頭型核醣核酸酵素(Hammerhead Ribozyme)及D型肝炎病毒核醣核酸酵素(Hepatitis D virus Ribozyme),期能順利切割感染性胰臟壞死病毒的核醣核酸。本實驗選用感染性胰臟壞死病毒的A段1346至1776的基因片段,經由不同的核酸限制酵素的切割,產生出不同的線形的質體,利用Sp6聚合?來進行活體外轉錄,合成出部份IPNV正股RNA,當做受質RNA;另一方面核醣核酸酵素的合成是直接利用含T7?動子序列之去氧核醣核酸模版,進行活體外轉錄反應。結果發現,欲取得多量的RNA時,必先審慎評估T7?動子下游之6個核?酸序列。在設計的14個核醣核酸酵素中,僅有RT-1與RT-3n兩個鎚頭型核醣核酸酵素能夠切割受質核醣核酸,此時的反應溫度為37℃及含12mM的MgCl2。同時為了要符合生物體內生理狀況則降低濃度的鎂離子的濃度由12mM至6mM,亦有不錯的反應效果。另一方面為了要提高切割標的RNA的反應成效,則嚐試著提高溫度,可使得原本於37℃無法進行切割反應的RT-7在50℃時可反應;然而適量地添加變性劑或PEG 6000亦可增加切割反應。 | zh_TW |
dc.description.abstract | Infectious pancreatic necrosis virus (IPNV) is contagious and widespread fish disease. In Taiwan aquaculture, IPNV has often caused heavy damage to the aquacultures industry for tilapia, eel, milk fish and clam. Development of effective control technology for IPNV infection has obviously become an important issue in fisheries development program and also imperative to continuing growth of culture industry. In this study, I designed highly specific hammerhead ribozyme and hepatitis delta virus ribozyme, synthesized the ribozyme RNAs and assayed the trans-cleavage activity of these ribozymes on the partial sense RNA of IPNV. Ribozyme RNAs were synthesized by T7 RNA polymerase transcription of synthetic DNA template. The downstream (+1 to +6) of T7 promoter was found to be a key factor to RNA production. Both RT-1 and RT-3n. (hammerhead ribozyme) were capable to cleave substrate RNA in 12mM MgCl2 at 37℃. To match in the physiological environment of organism, decreasing the concentration of magnesium ion from 12mM to 6mM obtained good results. On the other hand, in order to elevate the efficiency of cleavage on the target RNA, increasing the reaction temperature and adding denaturants or PEG 6000 can improve the cleavage reaction. RT-7 was able to cleave substrate RNA in 12mM MgCl2 at 50℃, that was uncut at 37℃. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:28Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
dc.description.tableofcontents | 中文摘要……………………………i 英文摘要……………………………ii 縮寫表……………………………1 壹、緒論 一 感染性胰臟壞死病毒之基本特性………………………3 (一)感染性胰臟壞死病毒宿主種類與臨床症狀……………3 (二)感染性胰臟壞死病毒之發現史………………………3 (三)感染性胰臟壞死病毒之生長與生化特性………………4 (四)感染性胰臟壞死病毒的遺傳物質與蛋白質的研究……4 (五)感染性胰臟壞死病毒的血清分類………………………6 二、核醣核酸酵素的基本簡介………………………………6 (一)Large catalytic RNA…………………………………7 (二)Small catalytic RNA…………………………………9 貳、實驗材料…………………………………………………13 一、生物材料………………………………………………13 二、反應試劑………………………………………………14 三、反應溶液………………………………………………16 參、實驗方法………………………………20 一、構築受質RNA表現載體………………………………20 二、試管內轉錄作用合成RNA……………………………24 肆、結果……………………………………29 伍、討論 ……………………………………35 陸、結論 ……………………………………39 柒、圖表……………………………………40 捌、參考文獻………………………………71 玖、附錄……………………………………77 | |
dc.language.iso | zh-TW | |
dc.title | 抗感染性胰臟壞死病毒核醣核酸酵素之研究 | zh_TW |
dc.title | A Study of Development Ribozyme Technology Resistance to Infectious Pancreatic Necrosis Virus | en |
dc.date.schoolyear | 84-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 84 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。