請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76240完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林德金 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:19:26Z | - |
| dc.date.available | 2021-07-01T08:19:26Z | - |
| dc.date.issued | 1996 | |
| dc.identifier.citation | 1. Hung, C. -H., Hung, K. -F. and Chiou, S. -H. (1994) Biochem. Biophys. Res. Commun. 165, 689-694 2. Hung, K. -F., Hung, C. -C., Pan, F. -M., Chow, L. -P., Tsugita, A. and Chiou, S. -H. (1995) Biochem. Biophys. Res. Commun. 216, 223-233 3. Teng, C. -M., Ko, F. -N., Tsai, I.-H., Hung, M. -L. and Hung, T. -F. (1993) Thromb. Haemost. 69, 286-292 4. Ho, C. -L., Teng, C. -M. and Lee, C. -Y. (1984) Toxicon 22, 813-816 5. Chen, L. -N., Liu, C. -S. and Chang, C. -C. (1994) Biotechnol. Appl. Biochem. 19, 61-73 6. Tsai, I. -H., Lu, P. -J., Wang, Y. -M., Ho, C. -L. and Liaw, L. -L. (1995) Biochem. J. 311, 895-900 7. Teng, C. -M., Wang, J. -P., Peng, H. -C. and Ouyang, C. (1989) Toxicon 27, 899-905 8. Liu, C. -S., Chen, J. -M., Chang, C. -H., Chen, S. -W., Teng, C. -M. and Tsai, I. -H. (1991) Biochem. Biophys. Acta 1077, 362-370 9. Wang, Y. -M., Lu, P, -J. and Tsai, I. -H. (1994) Journal of the Chinese Biochemical Society 23, 55-58 10. Ouyang, C., Teng, C. -M. and Huang, T. -F. (1992) Toxicon 30, 945-966 11. Ouyang, C. and Huang, T. -F. (1984) Toxicon 22, 705-718 12. Ouyang, C. and Teng, C. -M. (1976) Biochem. Biophys. Acta 420,298-308 13. Kondo, K., Narita, K. and Lee, C. -Y. (1978) J. Biochem. (Tokyo) 83, 91-99 14. Hendon, R. A. and Tu, A. T. (1976) Biochem. Biophys. Acta 578, 243-252 15. Viljoen, C. C., Botes, D. P. and Kruger, H. (1982) Toxicon 20, 715-737 16. Fohlman, J., Eaker, K., Karlsson, E. and Thesleff, S. (1976) Eur. J. Biochem. 68, 457-469 17. Nishida, S., Terashima, M., Shimazu, T., Takasaki, C. and Tamiya, N. (1985) Toxicon 23, 73-85 18. Hunter, W. M. and Greenwood, F. C. (1962) Nature (London) 194,495-496 19. Neville, D. M., Jr. (1971) J. Biol. Chem. 246, 6328-6334 20. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) J. Biol. chem. 193, 265-275 21. Whittaker, V. P. (1959) Biochem. J. 72, 694-706 22. de Haas, G. H., Postema, N. M., Nieuwenhuizen, W. and van Deenen, L. L. M. (1968) Biochem. Biophys. Acta 159, 103-117 23. Schanberg, S. M., Schildkraaut, J. J. and Kopin, I. J. (1967) J, Pharmacol. Exp. Therap. 157, 311-318 24. Schweitz, H. (1984) Toxicon 22, 308-311 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76240 | - |
| dc.description.abstract | TMVI是由龜殼花毒液純化得來的蛋白質，具有磷脂?A2活性，其分子量約為16,000。本文利用125I標示後的TMVI測出它在天竺鼠神經鍵體上的高親合力專一性結合。由數學作圖所求得的解離常數(Kd）為3.7×10-7M，而未碘化之TMVI抑制125I-TMVI結合之IC50則為5×10-8 M。很明顯地，碘化過程降低TMVI對天竺鼠神經鍵體細胞膜之親合力。碘化也會使TMVI的磷脂?A2活性下降45％。125I-TMVI在天竺鼠神經鍵體上之最大結合量（Bmax）為19.6 pmole/mg of protein。 以protease K或胰蛋白?處理天竺鼠神經鍵體，並不能影響125I-TMVI在天竺鼠神經鍵體上之結合，因此，結合體可能並非蛋白質。而以Sr2+離子取代Ca2+離子，125I-TMVI仍有結合能力，因此125I-TMVI在天竺鼠神經鍵體上的專一性結合並不需要PLA2活性。某些其他PLA2也能抑制125I-TMVI在天竺鼠神經鍵體上的專一性結合，惟其抑制能力不一。 | zh_TW |
| dc.description.abstract | TMVI, a PLA2 with molecular weight of 16,000 was purified from Trimeresurus mucrosquamatus venom. TMVI was radioactively iodinated and used to demonstrate specific binding to synaptosomes. The dissociation constant (Kd) calculated from the double-reciprocal plot of the binding curve is 3.7*10-7M. For comparision, unlabeled TMVI inhibited the binding of 125I-TMVI with an IC50 of 5*10-8M. Obviously, iodination of TMVI affect the protein to some degree. The iodination also caused 45% decrease in the PLA2 activity of TMVI. The number of binding sites in the synaptosome is 19.6 pmole/mg of protein. Because the binding activity of 125I-TMVI to synaptosomes is resistant to proteolysis, the binding site may be other than proteins. Competition experiments showed that several other PLA2 variants can displace 125I-TMVI binding with the different ability. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:26Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
| dc.description.tableofcontents | 中文摘要------------------------1 英文摘要------------------------2 緒論----------------------------3 材料與藥品----------------------5 儀器----------------------------6 實驗方法------------------------7 實驗結果-----------------------16 討論---------------------------23 圖表---------------------------28 參考文獻-----------------------73 | |
| dc.language.iso | zh-TW | |
| dc.title | TMVI 在天竺鼠神經鍵體上之專一性結合 | zh_TW |
| dc.date.schoolyear | 84-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 78 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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