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DC 欄位 | 值 | 語言 |
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dc.contributor.author | 劉棠必 | zh_TW |
dc.date.accessioned | 2021-07-01T08:19:20Z | - |
dc.date.available | 2021-07-01T08:19:20Z | - |
dc.date.issued | 1996 | |
dc.identifier.citation | Alhertvn. J.. Hohrnann, S., Thevelein, J M. and Prior, BA. GPD1 ,which encodes glycerol-S-phosphate dehydrogenase,is essential for rowth under osmotic stress in Saccharomyces ceresvisiue.and its expression is regulated by the high-osmolarity glycerol response pathway. Mol.Cell.Biol. 14-6, 4135 - 4144. (1994) Andrews, P. The Gel-Filtration Behaviour of Protein Related to their Molecular Weights over a Wide Range. Biochem. J. 96, 595 - 606. (1965) Avernann, K., Knippers, R.,Koller, T. and Sogo. J.M. Carnptothecin,a specific inhibitor of type I DNA topoisomerase ,induceds DNA breakage at replication forks. Mol.Cell.Biol. 8-8, 3026 - 3034. (1988) Badaracco, G., Landsberger, N. and Benfante. R. Purification and Characterization of a proteolytic active ftagment of DNA topoisornerase I from the brine shrimp Artemia Franciscan (Crustacea anostraca). Biochem. J. 282, 249 -254. (1992) Bailey, G.S., Hendricks, J.D., Nixon, J.E. and Pawlowski. N.E. The sensitivity of rainbow trout and other fish to carcinogens. Drug Metabolism Review 15-4,725-750. (1984) Bailey, G.S., William, D.E., Wilcox, J.S., Loveland, P.M. Coulombe, R.A. and Hendricks, J.E. A flatoxin Bi carcinogenesis and its relation to DNA adduct fonnation and adduct persistence in sensitive and resistant salmonid fish. Carcinogenesis 9-11, 1919 - 1926. (1988) Baker, S.D., Wadkins, R.M., Stewart, C.F., Beck. W.T. and Danks, M.K. Cell cycle analysis of amount and distribution of nuclear DNA topoisomerase I as determined by fluorescence digital imaging microscopy. Cytometry 19, 134 - 145. (1995) Boguslawski. G. PBS2, a yeast gene encoding a putative protein kinase. interacts with the RAS2 pathwav and affects osmotic sensitivity of Saccharomyees. Cerevsiae. J.Gen. Microbiol. 138. 2425 - 2432. (1992) Braitiuan. D. L. Bomstein, P. and Gallis, B. Phosphotvrosvl -protein phosphatase. Specific inhibition by Zn. J. Biol. Chen 256- 13,6519-6522. (1981) Brewster, .J.L.. de Valoir, T., Dwyer, ND., Winter,E. and Gustin, M.C. An osmosensing signal transduction pathway in yeast. Scicnce 259, 1760- 1763. (1993) Brunati,A.M. and Pinna,L.A. Isolation and partial characterization of distinct species of phosphotvrosv Iprotein phosphatase from rat spleen. Biochem.Brophys.Res.Comrn.. 133-3, 929 -936. (1985) Burg, MB. Molecular basis of osmotic regulation. The American Physiological Society F983—F996. (1995) Cardellin. E. and Durban, E. Phosphorviation of human topoisomerase I by protein kinase C in vitro and in phorbol 12-myristate 13-acetate-activated HL-60 prornylocvtic leukaernia cells. Biochem .J. 291, 303 -307. (1993) Carpenter. G.. King,L.Jr, and Cohen, S. Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. J.Biol.Chem. 254-11,4884-4891. (1979) Chackalaparampil. I. and Shalloway,D. Altered phosphorylation and activation of pp60cs during fibroblast mitosis. Cell 52,801-810. (1988) Charbonneau. H. and Tonks, N.K. 1002 protein phosphatases Annu. Rev.Cell Riol.8. 463-493. (1992) Charhonneau. H., Tonks, N.K., Kurnar, S., Diltz. CD., Ilarrvlock. M.. Cool. D.E.. Krebs, E.G.. Fischer,E.H. and Walsh,K.A. Human placenta protein-tyrosine- phosphatase ,amino acid sequence and relationship to a family of receptor-like proteins. Proc..Nal..Acad..Scl.USA.86, 5252 - 5256. (1989) Coughlin, SR.. Barr, P.3., Cousens, L.S.. Fretto,L.J. and Williams, L.T. Acidic and basic fibroblast growth factors stimulate tvrosme kinase activity in vivo. J.Bioi.Chem. 263-2, 988- 993. (1988) Courtneidge. S.A. and Furnagalli, S. A initotic function for src Trends in Cell Biol. 4, 345-347. (1994) D’Arpa. P. and Lat, L.F. Cell cycle - specific and transcription - related pliosphorvlation of mammalian DNA topoisornerase 1. Experimemal Cell Research 217, 125 - 131. (1995) Devarv, Y.. Gottlieb, R.A., Smeal, T. and Karin, M. The mammalian ultraviolet response is triggered by activation of src tyrosine kinase. Cell 71, 1081-1091(1992) Domanico. P.L. and Tse-Dinh. Y.C. Mechanistic studies of F. coli DNA topoisomerase I , divalent ion effects. .J. Inorg. Biochem. 42, 87 - 96. (1991) Durban, E.. Goodenough, M., Mills, J. and Busch. H. Topoisomerase I phosphorylation in vitro and in rapidly growing Novikoff hepatoma cells. The EMBO.Jonrnal. 4-11, 2921-2926. (1985) Durban. E.. Mills. J.S.. Roll, D. and Busch, HF. Phosphorviation of purified novikoff hepatoma topoisomerase I. Biocliem. Biophis. Res. Comm. 111, 897 — 905. (1983) Egyhazi, E. and Durban, E. Microinjection of anti—topoisomerase immunoglobulin G into nuclei of Chironomus tentans salivary gland cells leads to blockage of transcription elongation Mol. Cell. Biol. 7,4308-4316. (1987) Erpel. T. and Courtneidge. S.A. Src family protein kinases and cellular signal transduction pathways. Curr. Opi. in Cell. Biol. 7, 176- 182. (1995) Feng. G.S., Hui, CC. and Pawson, T. SH2-contamting phosphotyrosine phosphatase as a target of proteintyrosine kinases. Science 259, 1607-1611. (1993) Fleischmann. G.. Pflugfelder, G, Steiner, E.K., Javaherian. K.. Howard. G.C., Wang, J.C. and Elgin. S. Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome. Proc. Nall..Acad.Sci.USA.81, 6958-6962. (1984) Frangioni. J.V., Beahrn, PH., Shifrmn, V., Jost. C.A. and Ned, B.G. The nontrausmembrane tyrosine phosphatase PTP-I B localization to the endoplasmic reticulum via its 35 amino acid C-terminal sequence. Cell 68, 545 - 560. (1992) Gallis, B.. Bornstein, P. and Brautigan, D.L. Tyrosyiprotein kinase and phosphatase activities in membrane vesicles from normal and rous sarcoma virus-transformed rat cells. Proc. Nail. Acad. Sci USA. 78, 6689- 6693. (1981) Gelkop, S., Priel, E. and Isakov, N. T cell mitogens induce increased DNA topoisomerase I protein levels and its translocation to the nucleus where activation of he enzyme occurs. Biochem. Biophys. Res. Commnu.. 11-3,872-877. (1995) Gong, C. X.. Singh, T.J., Grundke-Iqbal, I. and Iqbal, K. Phosphoprotem phosphatase activities in Alzheinmer disease brain. Journal of Neurochemistry 61-3, 921 - 927. (1993) Gupta. M Fulimori, A. and Pommier. Y. Eukarvotic DNA topoisomerases I. Biochrm et Biophys. Acta. 1262, 1 - 14. (1995) Harlow. E and Lane. D. Antibodies, A Laboratory Manual Cold Spring Harbol Laboratory (1988) Heck. M. S.. Hittelman. W.N. and Earnshaw. W.C. Differential expression of DNA topoisornerase I and II during eukaryotic cell cycle Proc. Nall. .Acad. Sci. USA. 85, 1086 - 1090. (1988) Heldin. C.H. arid Westermark, B. Growth factors . mechanism of action and relation to oncogenes. Cell 37.9-20 (1984) Hoerlem. D., Gallis, B., Brautigan, D.L. and Bornstein, P. Partial purification and characterization of phosphotyrosyl-protein phosphatase from ehrlich ascites tumor cells. Biochemristry 21. 5577 - 5584. (1982) Hsiang. Y. H. and Liu. L.F. Identification of mammalian DNA topoisomerase I as an intracellular target of the anticancer drug camptothecin. Cancer Research 48. 1 722 - 1726. (1988) Hunter, T. Protein kinase and phosphatase,the yin and yang of protein phosphorviation and signaling. Cell 80. 225 -236. (1995) Hunter. T. and Sefton. B.M. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc. Nall. Acad. Sci.. 77, 1311 - 1315. (1980) Hwong, C. L.. Chen, C.Y., Shang, HF. and Hwang, J. Increased synthesis and degradation of DNA topoisornerase I during the initial phase of human T lymphocyte proliferation. J.Biol.Chem.. 268-25. 18982- 18986. (1993) Hwong. CL.. Chen, MS. and F-Iwang, J. Phorhol ester transiently increase topoisornerase I mRNA levels in human skin fihrohlasts. J. Biol. Chem. 264-25, 14923 - 14926. (1989) Thie. J.N., Witthulrn, B. A., Queue, F.W., Yarnamoto, K. and Silvennoinen, 0. Signaling through the hematopoietic cvtokine receptors. Annu. Rex. lmmunol.. 13, 369 - 398. (1995) Ishimi, Y., Nashizawa. M. and Andoh. T. Characterization of a camptothecin- resistant human DNA topoisornerase I in an in vitro system for Simian virus 40 DNA replication. Eur. .J. Biochem. 202, 835 - 839. (1991) Johnson. P., Ostergaard. H.L., Wasden. C. and Trowbridge. I.S. Mutational analysis of CD45. A leukocvte-specific protein tyrosine phsophatase. J. Biol. Chem. 267,8035-8041. (1992) Jones. S. W.. Erikson, R. L., Ingebritsen, V.M. and Ingebritsen, T.S. Phosphotvrosvl -protein phosphatase. J. Biol. Chem. 264-13, 7747-7753.(1989) Kaguni. J. M. and Kornberg, A. Topoisoinerase I confers specificity in enzymatic replication of the Escherich Ia coli chromosomal origin. J. Biol. Chem.. 259, 8578-8583. (1984a) Kaguni, J. M. and Kornberg, A. Replication initiated at the origin (oriC) of the E. coli chromosome reconstituted with purified enzymes. Cell 38, 183-190. (984b) Kaisennan, H.B., Ingebritsen, T.S. and Benbow, R.M. Regulation of Xenopus laevis DNA topoisomerase I activity by phosphorylation in Vitro. Biochemistry 27, 3216 -3222. (1988) Kasid, U.N.. Halligan, B., Liu, L.F., Dritschilo, A. and Smulson, M. Polv( ADP-ribose)-rnediated post-translational modification of chromatin-associated human topoisomerase I. J. Biol. Chem. 264. 18687- 18692. (1989) Kim, R.A. and Wang. .1.C. Function of DNA topoisornerases as replication swivels in .Saccharomyces Function of DNA topoisomerases as replicaion swivels in Saccharomyces cerevisiae J. Mol. Chem. 208. 257 - 267. (1989) King, Mi. and Sale, G.J. Assay of phosphotyrosyiprotein phosphatase using synthetic peptide 1142- 1153 of the insulin receptor. EEBS. Len. 237. 137 - 140. (1988) Kordivak, G. J., Jakes, S., Ingebritsen, T.S. and Benbow, R.M. Casem kinase 11 stimulates Xenopus laevis DNA topoisornerase I by physical association. Biochemistry. 33, 13484-13491 . (1994) Kunze, N., Yang. G,. Dolberg, M.. Sumdarp, R., Knippers. R. and Richter, A. Structure of the human type I DNA topoisomerase gene. J. Biol. Chem. 266. 15, 9610 -9616. (1991) Kretzschrnar, M., Meisterernst, M. and Roeder, R.G. Identification of human DNA topoisomerase I as a cofactor for activator- dependent transcription by RNA polyrnerase II. Proc.Nall.Acad.Sci.USA. 90, 11508- 11512. (1993) Larnmli, U.K. Cleavage of Structure Proteins during the Assembly of the Head of Bacteriophage T4 Nalure 227-15.680-685. (1970) Lane, L.C. A Simple Method for Stablizing Potein-Sulthydrl Groups during SDS-Gel Electrophoresis .Analytical Biochemistry 6, 655 - 664. (1978) Law W.K.H., Farley, J.R. ann BaylinK, D.j. Phosphotyrosyl protein phosphatase. Biochem. J. 257, 23 - 36. (1989) Lan, W.K.H., Freeman, T.K. and Baylink, D.J.. Purification and characterization of an acid phosphatase that displays phosphotvrosyl-protein phosphatase activity from bovine cortical bone matrix. J. Biol. Chem. 262-3, 1389- 1397. (1987) Law W.K.H., Freeman, T.K. and Baylink, D. J. Purification and Characterization of an Acid Phosphatase That Displays Phosphotvrosyl-protein phosphatase Activity from Bovinc Cortical Bone Matrix. J. Biol. Chem. 262, 1389- 1397. (1987) Lee. M.P., Brown, S.D. and Hsieh, T.S. DNA ropoisomerase I is essential in Drosophila .Malaogu.ster. Proc Nall.Acad. Sci.. 90, 6656 - 6660. (1993) Liu. L.F. and Miller, K,G. Eukarvoric DNA topoisomerases I, Two Forms of Types I DNA Topoisornerases from HeLa Cell Nuclei. Proc.,Nall.Acad. Sci. USA. 78,3487-3491, (1981) Luna, E.J. and Hitt, A.L. Cytoskeletol-plasma membrane interactions Science 258, 955 - 964. (1992) Martineau. D., Bowser,P.R., Renshaw, R.R. and Casey, J.W. Molecular characterization of a unique retrovirus associated with a fish tumor. J. virol. 66-1, 596 - 599. (1992) Mauro L.J. and Dixon J.E. Zip codes’ direct intracellular protein tyrosine phosphatases to the correct cellular ‘address’. TIBS. 19. 151 - 154. (1994) McCormick A. Miles, L.E.M., Chrambach, A. Selective Elution of Zones from Preparative Isoelectric Focusing Gel by Ainpholvtes or Buffer. Analyucal Biochemistry 75. 321 - 324. (1976) McLaughlin, S. and Dixon, J.E. Alternative splicing gives rise to a nuclear protein tvrosine phosphatase in Drosophila. J. Biol. Chem. 268, 6839- 6842. (1994) McMalion, G. The genetics of human cancer, implications for ecotoxicologv. Emvironmental Health Perspectives. 102-12,75 -80. (1994) Merino, A., Madden. K.R., Lane, W.S.. Champoux. J.J. and Reinberg, D. DNA topoisornerase I is involved in both repression and activation of transcription. Nalure 365, 227-232. (1993) Merril, C.R., Goldman, D., Sedman, S.A. and Ebertm. M.H. Ultrasensitive stain for proteins in polyacrvlamide gels shows regional variation in cerebrosoinal fluid proteins. Science 221, 1437- 11438. (1981) Mourey, R.J. and Dixon J.E. Protein tyrosine phosphatases, characterization of extracellular and intracellular domains. Current Opinion in Genetics and Development 4, 31 - 39. (1994) Muller, M.T., Pfund, W.P., Mehta, V.B. and Trask, D.R. Eukaryotic type I topoisomerase is enriched in the nucleolus and catalytically active on ribosomal DNA. The EMBO J. 4, 1237- 1243. (1985) Murchelano, R.A. and Wolke, R.E. Epizootic carcinoma in the winter flounder, Pseudopleuronectes americanus. Science 228, 587 -589. (1985) Nelson. R.L. and Branton. P.E. Identification, purification and characterization of phosphotvrosine specific protein phosphatases from cultured chicken embryo fibroblasts. Mol. Cell. Biol. 4, 1003 - 1012 (1984) Norburv. C. and Nurse, P. Animal cell cycles and their control. Annu Rev. Bichem.. 61, 441- 470. (1992) Paartanen, S. and Pekonen, F. Histochemicallv demonstrable phosphotvrosvl-protein phosphatase in nonnal human breast, in benign breast diseases and in breast cancer. Anncancer research 9, 667 - 672. (1989) Park, S.M. and Koo, H-S. Purification of Caenorhabditis elegans DNA topoisomerase I. Biochinuca et Biophysica .Acta.. 1219, 47 - 54. (1994) Pawson. T. and Gish, G.D. SH2 and SH3 domains , from structure to function. Cell. 71.359-362. (1992) Pliss, GB. and Khudoley, V.V. Tumor induction by carcinogenic agents in aquarium fish. Journal of the .National Cancer Institute 55-1, 129 - 136. (1975) Pommier, Y., Kerrigan, D., Hartman, K.D. and Glazer, R.I. Phosplioiylation of mammalian DNA topoisomerase I and activation by protein kinase C. J. Riol. Chem. 265, 9418 -9422. (1990) Pommier, Y. Eukarvotic DNA topoisomerase I, genome gatekeeper and its intniders, camptothecins. Seminor in Oncology 23-1, 3-10. (1996) Poot, M.. Hiller, K-H., Heimpel, S. and Hoehn. H. Distinct patterns of cell cycle disturbance elicited by compounds interfering with DNA topoisomerase I and II activity. Experiniental Cell Research 218,326-330. (1995) Rose, K.M., Szopa. J.. Han. P.S., Cheng, Y.C., Richter,A. and Scheer, U. Association of DNA topoisomerase I and RNA polymerase La possible role for topoisomerase I in ribosornal gene transcription. Chomosoma 96.411 - 416. (1988) Rossi. F . Lahouner. E.. Forne, T., Divita. G. Derancourt, J., Riou, J.F., Antine. F., Cathala. C.. Brunri C. and Tazi J. Specific phosphorviation of SR proteins by mammalian DNA topoisoinerase I Nater 381. 80 - 82. 1996 Sambrook J. Fritsch. E.F. and Maniatis, T. Molecular Cloning A Laborator Manual (2nd edition), Cold Spring Harbor Laboratory. (1989) Samuel. D.S.. Shimizu. Y. and Shimizu, N. Protein kinase C phosphorvlates DNA topoisornerase I. FEBS Lell.. 259. 57 -60. (1989) Samuels. ID S. and Shimizu, N. DNA topoisomerase I phosphorylation in murine fibroblasts treated with 12-O-tetradecanoviphorbol- 13-acetate and in Vitro by protein kinase C. J. Biol. Chem. 267, 11156- 11162. (1992) Sarnules. D.S.. Shimizu. Y., Nakabayashi, T. and Shirnizu. N Schlessinger,J. SH2/SH3 signaling proteins. Biochimica et Biophica Acta, 1233, 77 - 83. (1994) Schuller, C., Brewster. J.L., Alexander, M.R., Gustin, M.C. and Ruis, H. The HOG pathway controls osmotic regulation of transcription via the stress response elernent(STRE) of the Saccharomyces cerevisiae CTTI gene. The EMBO .J. 13 - 18. 4382 -4389. (1994) Smith. P.K., Krohn, R.I., Henianson, C.T., Mallia, A.K., Gartner, F.H. and Provenzana. D.C. Mearsurement of Protein Using Bicinchoninic Acid Ana1.Biochem I 50. 76 - 85. (1985) Superti-Furga, G. and Courtneidge, S.A. Structure-function relationships in Src family and related protein tvrosine kinases. Brassays 17, 321 - 330. (1995) Tamura. S., Suzuki. Y., Kiktichi, K. and Tsuiki, S. Identification and characterization of Mg2-dependent phosphotvrosyl protein phosphatase from rat liver cytosol. Btochem. Biophys. Res. Comm.. 140-1, 212 - 218. (1986) Tauchi T., Feng, G.S., Shen. R., Hoatlin. M., Bagbv, Jr.G.C., Kabat, D., Lu. L. and Broxrneyer, HE. Involvement of SH2-containing phosphotvrosine phosphatase Syp in ervthropoietin receptor signal transduction patlnays. J. Biol. Chem. 270, 5631 -5635. (1995) Taylor. S.J. and Shalloway, D. The cell cycle and c-Src. Currem Opinion in Cenetics and Developnicnt 3. 26 - 34. (1993) Thivagarajan, MM., Kotani, H., Holloman, W.K. and Kmiec. E.B. DNA relaxation mediated by Usti1agy maydis type I topoisomerase; modulation by chromatin associated proteins. Biochem et Biophysica Ada. 1173, 155 - 164. (1993) Thrash, C., Voelkeo, K., Dinardo, S. and Sternglanz, R. Identification of saccharomyces cerevusuae mutant deficient in DNA topoisornerase I activity. J. Biol. Chem. 259, 1375 - 1377. (1984) Tillrnann, U., Wagner, J., Boerboom, D., Westphal, 1-1. and Tremblay, M.L. Nuclear localization and cell cycle regulation of a murine protein tyrosine phosphatase. Mol.Cell.Biol. 14-5,3030-3040. (1994) Tornic. S.. Greiser, U. Lammers, R., Kharitonekov, A.. lrnvanitov. E., Ullrich, A and Bohmer, F.D. Association of SH2 domain protein tyrosine phosphatases with the epidennal growth factor receptor in human tumor cells. J. Biol. Chem. 270,21277-21284. (1995) Tonks, N.K.. Diltz, CD. and Fischer, E.H. Purification of the major protein-tvrosine -phosphatases of human placenta. J. Biol. Chem. 283, 6722- 6730. (1988) Tournier, M.F., Sobczak, J.. Nechaud, B. and Duguet, M. Comparison of biochemical properties of DNA topoisomerase I for normal and regenerating liver. Enr. J. Biochem. 210, 359 - 364. (1992) Tsao, Y.P., Wu, H.Y. and Liu, L.F. Transcription-driven supercoiling of DNA,direct biochemical e idence from in vitro studies. Cell 56. 111 - 118. (1989) Tse-Dinh. Y-C., Wang, T.W. and Goldberg, A.R. Virus- and cell-encoded tyrosine protein kinases inactivate DNA topoisomerase I in vitro. Nature 312. 20- 27. (1984) Tung, H Y and Reed, L.J. Identification and purification of a cytosolic phosphotvrosvl protem phsophatase from bovine spleen. Anal. Biochem. 161, 412-419. (1987) Turman rvl. A. and Dauvas A. A casein kinase type II (CKII)-like nuclear protein kinase associates with, phosphorviates, and activates topoisomerase I Biomedical medicine and metahilc bio1ogy 50, 210-225. (1993) Uemura. T. and Yanagida, M. Isolation of type I and II DNA topoisomerase mutants from fission yeast, single and double mutants show different phenotype in cell growth and chromatin organization. EMBO J, 3.1737- 1744. (1984) Vicendo P., Fauvel, J., Ragab-Thornas, J.F. and Chap, H. ldentitication,characterization and purification to near-homogeneity of a novel 67 kDa phosphotyrosyl protein phosphatase associated with pig lung annexin extract. Biochem .J. 278, 435 - 440. (1991) Walton, KM. and Dixon, J.E. Protein tvrosine phosphatases. Annu. Rev. Riochem. 62, 101 - 120. (1993) Wang, J.C. Interaction between DNA and an Escherichia coli protein. J .Mol. Riol. 55. 523 - 533. (1971) Wan, J.C. DNA topolsornerases. Annu. Rev. Biochem. 54, 665 - 697. (1985) Wang J.C. Recent studies of topoisomerase I Biochem et Biophysica Acia. 909, 1 - 9. (1987) Wang, iC. and Lynch. AS. Transcription and DNA supercoiling. Curr. Opt. in Gen. Dev. 3, 764 - 768. (1993) Welch, P.J. and Wang, J.Y.J. A C-tenriinal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle. Cell 75, 779 - 790. (1993) Wilson, R. et al. 2.2 Mb of contiguous nucleotide sequence from chromosome 111 of Celegans. Naiure 368, 32-39. (1994) Witt,D.P. and Gordon, J.A. Specific dephosphorylation of membrane proteins in Rous sarcoma virustransformed chick embryo fibroblast. Nalurc 287, 241 - 233. (1980) Wu, N.Y., Shvy, S.H., Wang, J.C. and Liu, L.F. Transcription generates positively and negatively supercoi led domains in the template. Cell 53. 433-140. (1988) Yew, F.H. and Chang, L.M. DNA replication and repair in Tidapia cells. Journal of Cell. Scrence 72, 213 -216. (1984) Zhang. ZY. Wang. Y.. WU. L.. Faurnan, E.B., Stuckey, J.A., Schubert. H. L., Saper. M.A. and Dixon. J.E. The Cvs( X)Arg catalytic motif in phosphoester hydrolysis. Biocheinisirr 33. 15266 - 1520. (1994b) Zhang, Z.Y. Wang Y. and Dixon, J.E. Dissecting the catalytic mechanism of protein-tyrosine phosphatases. Proc. Nail. Acad. Sci. 91, 1624- 1627. (1994a) Zhou, C.. Agarwal. N. and Cammarata, P. Osrnoregulatorv alterations in rnyo-inositol uptake by bovine lens epithelial cells.1l.cloning of a 626 bp cDNA portion of a Na/rnyo-inositol cotransporter. an osmotic shock protein. Invest. Ophuhulmol. Vls. Sci. 35, 1236 - 1242. (1994) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76230 | - |
dc.description.abstract | 本論文採用水生脊椎動物:Tilapia mossambica為實驗材料,對魚類細胞中影響酪胺酸磷酸化的蛋白質酪胺酸解磷酸?與近來受到熱烈討論並成為化學療法對象的拓樸異構?-I進行活性調控的研究。 純化的步驟共使用1次親合力層析法,2次陰離子交換層析法與1次膠體過濾層析法。純化到的核型PTPase其比活性為5.095unit/mg of protein。分子量為62kDa(由膠體過濾管柱SuperoseTM 12層析法推算)與67kDa與60kDa(由SDS-PAGE推算)。pI值為5.79±0.02。pH 7.0為酵素活性最適作用酸鹼值。Ca2+、Mg2+、Mn2+與VO43-與Zn2+五種金屬離子對純化到之核型蛋白質酪胺酸解磷酸?活性的影響皆為抑制效果其中Mn2+,VO43-與Zn2+對PTPase活性具有很強的的抑制作用。 依據等當於蛋白質酪胺酸解磷酸?純化步驟所得到之拓樸異構?-I的比活性為53.000unit/mg of protein。由SDS-PAGE估算之分子量為66kDa,65kDa,64kDa與58kDa,並且可被anti human topoisomerase I-antibody辨認。 純化得到的拓樸異構?-I在事先未經任何處理的狀態下,經由人類鹼性解磷酸?作用後活性幾乎消失,而核型PTPase對於此一狀態的拓樸異構?-I並無影響活性的作用。但若經c-Src以[γ-32P]-ATP封拓樸異構?-I進行磷酸化後,一方面可在放射性自動顯影中觀察到pl由5.32,5.23,5.11與4.93往酸端移動成為4.32,4.23,4.06與3.96,另方面造成拓樸異構?-I的活性下降,之後再經過核PTPase解磷酸化作用後,拓樸異構?-I的解旋能力回復至原來活性。c-Src與核蛋白質酪胺酸解磷酸?對拓樸異構?-I的作用關係與發生的可能性進一步在本論文中討論。 | zh_TW |
dc.description.abstract | A phosphotyrosyl protein phosphatase (PTPase) was purified from the hepatic nuclear matrix of Tilapia mossambica with a final specific activity of 5.095 units/mg of protein. 2% CHAPS and 2 M NaCl were applied to extract the matrix bound protein and one affinity column (HiTrap Heparine). two anion exchangers (HiTrap Q and Mono Q HR 5/5) plus one gel filtration column (SuperdexTM 200) were conducted consecutuvely to purify the enzyme. The PTPase had a relative molecular mass of 67 kDa and 60 kDa as estimated by SDS-PAGE and of 62 kDa by gel filtration chromatography. This monomeric and neutral PTPase was with an isoelectric point of 5.79 ± 0.02 and an optimal activity at pH 7.0 in the dephosphorylation of a 32P-labeled synthetic insulin receptor peptide [ -RDI(PY)ETD(PY)(PY)R- ] as substrates. Orthovanadate, Zn2+ and Mn2+ were strong inhibitors for the purified PTPase and had IC50 at the level of μM; Mg2+ and Ca2+ had higher IC50 in mM level. Through comparable purification procedures, topoisomerase I was purified in proteolytic forms with relative molecular mass of 66 kDa. 65 kDa and 59 kDa, and had a final specific activity of 53,000 units/mg of protein. The influence of different topoisomerase I phosphorylation states on the enzyme activity was investigated: incubation of the purified topoisomerase I with human alkaline phosphatase abolished the relaxing activity in a time-dependent pattern but not with PTPase. The activity of topoisomerase I was also inhibited (49% decreased) by recombinant human c-Src-mediated tyrosyl phosphorylation and re-activated by PTPase-treatment. In isotope labeling experiments which employed the purified topoisomerase I, [γ-32P] ATP and recombinant human c-Src, a pI-shifting was observed in Immobiline DryStrip Gel by isoelectric focusing. The interaction between Src, topoisomerase I and PTPase was futher discussed. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:19:20Z (GMT). No. of bitstreams: 0 Previous issue date: 1996 | en |
dc.description.tableofcontents | 中文摘要 Ⅰ 英文摘要 Ⅱ 縮寫表 Ⅲ 序論 1 材料與方法 10 實驗材料 一.生物材料 10 二.藥品與試劑 10 三.反應組與器材 12 實驗方法 一.酵素純化步驟 13 二.蛋白質磷酸化酪胺酸解磷酸?之活性分析 16 三.拓樸異構?-I之活性分析 17 四.質體DNA的大量製備 18 五.鹼性解磷酸?對拓樸異構?-I活性調控之分析 19 六.酪胺酸磷酸化及解磷酸化反應對拓樸異構?-I活性調控之分析 19 七.膠體過濾層析法估算分子量 20 八.蛋白質定量 21 九.聚丙烯醯胺膠體電泳 21 十.銀染法 22 十一.西方免疫點墨分析 22 十二.等電集焦電泳 23 十三.放射性自動顯影 24 結果 25 討論 31 展望 38 參考文獻 40 圖表 55 | |
dc.language.iso | zh-TW | |
dc.title | 吳郭魚肝臟細胞之核型蛋白質磷酸化酪胺酸解磷酸?以c-Src磷酸化的拓樸異構晦-I為受質 | zh_TW |
dc.title | c-Src Phosphorylated Topoisomerase I as a Substrate of Nuclear Phosphotyrosyl Protein Phosphatase from the Liver of Tilapia mossambica | en |
dc.date.schoolyear | 84-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 97 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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