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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林燦樑 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:18:41Z | - |
| dc.date.available | 2021-07-01T08:18:41Z | - |
| dc.date.issued | 1995 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76164 | - |
| dc.description.abstract | 黑鯛為一先雄後雌的雌雄同體魚種，在生殖季時可行多次連續或不連續的產卵，其生殖調控機制是由中樞神經系統及內分泌系統所調控，藉由雌二醇控制卵黃生成作用的進行。本實驗以雌二醇處理雄魚，取其mRNA進行反轉錄，經和對照組比較後，發現於21kb處有強烈的差異反應，其極有可能是Vg-mRNA，於是加以分離、純化、切割、大量複製，並加以定序後，得到一條約370bp的cDNA probe。以此探針，用以探知誘發狀態下魚體肝臟內Vg-mRNA/total-RNA的相對比值，由實驗結果發現，處理組的Vg-mRNA/total-RNA比值，於第二次刺激後3hr-6hr到達高峰，且比對照組高過數十倍，實驗的雌二醇處理劑量以lmg/lkg B. W．反應最佳，另外，也以甲狀腺素單獨處理及與二者混合處理，結果發現，單獨處理甲狀腺素有些微的作用，混合處理時則有強大的交互作用，其使高峰更高且往前移，意味著甲狀腺素是一個正向的反應因數。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:18:41Z (GMT). No. of bitstreams: 0 Previous issue date: 1995 | en |
| dc.description.tableofcontents | 目錄 壹：摘要…………………………………………………………………………1 貳：前言…………………………………………………………………………2 參：材料與方法 1 實驗動物…………………………………………………………………………7 2 Total RNA的抽取……………………………………………………………………8 3. mRNA 的分離與純化及反轉錄實驗…………………………………………………………9 4 將ss-DNA轉製成ds-DNA (Nick reaction)及限制酵素內切反應……………………11 5 接合反應(Ligation)及聚合連鎖反應(PCR)…………………………………………12 6 標定(Labeling)，南方點墨分析(Southren Hybridization), 核酸定序(Sequencing)………13 7 定量分析……………………………………………………………………………15 肆:實驗結果 1 Total RNA的分離及純化…………………………………………………………16 2. mRNA的分離與純化及反轉錄實驗…………………………………………………17 3 將ss-DNA轉製成ds-DNA(Nick reaction)及限制酵素內切反應…………18 4 接合反應(Ligation)及聚合連鎖反應(PCR)…………………………………………18 5 核酸定序(Sequencing)及南方點墨分析(Southern Blot)……………………19 6 定自製探針之專一性…………………………………………………………21 7 Estrodiol-induced訊息核糖核酸之表現及Thyroxine之作用……………………21 伍：討論…………………………………………………………………23 陸：參考文獻……………………………………………………………34 柒：圖表……………………………………………………………………40 | |
| dc.language.iso | zh-TW | |
| dc.title | 雌二醇誘導黑鯛肝臟中雌性特有訊息核糖核酸生成之初步研究 | zh_TW |
| dc.title | Preliminary Study on Female-specific mRNA Synthesis Induced by Estradiol in the Liver of the Black Porgy(Acanthopagrus schlegeli) | en |
| dc.date.schoolyear | 83-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 53 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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