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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Jean-Leon Chong | en |
| dc.contributor.author | 章健良 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:18:00Z | - |
| dc.date.available | 2021-07-01T08:18:00Z | - |
| dc.date.issued | 1994 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76095 | - |
| dc.description.abstract | 為了對受光蛋白有更深的認識，尤其是彩色受光蛋白(cone pigment)。本實臉用金魚綠色受光蛋白核?酸(goldfish green cone pigment II cDNA)的第552個核?酸到第1364個核甘酸，長約0.8Kb 的DNA作為探針。從鯉魚視網膜cDNA基因庫(cDNA library)中篩選到鯉魚green cone pigment部份核?酸序列及第二種rhodopsin。 完成定序的部份鯉魚putative green cone pigment，核?酸序列長度為772個核?酸。與金魚green cone pigment I，第612到第1151核?酸有94.4％相同；與金魚green cone pigment II，第574到1337個核?酸有90.8％相同。蛋白質轉譯區由第2個核?酸到第482個核?酸，共160個胺基酸。與金魚green cone pigment I，從N-端第190個胺基酸到第349個胺基酸，有95.6％相同：與金魚green cone pigment II，從N-端第190個胺基酸到第349個胺基酸，有93.8％相同。 完成定序的部份鯉魚rhodopsin II，有1253個核?酸；與鯉魚rhodopsin I的第378個核?酸到第1532個核?酸有97.2％相同。在3’端非蛋白質轉譯區，鯉魚rhodopsin II比rhodopsin I多99個核?酸。兩者胺基酸轉譯區有三個胺基酸不一樣。rhodopsin I的第108個胺基酸Ile在rhodopsin II為Val. Rhodopsin I的第169個胺基酸Val在rhodopsin II為 Glu. rhodopsin I的第315個胺基酸His在rhodopsin II為Asn。 我們另外選殖到一段類似人、牛endozepine的cDNA；長度為510個核?酸。5’端非蛋白質轉譯區有42個核?酸。蛋白質轉譯區由第43個核?酸到第303個核?酸，共261個核?酸，轉譯87個胺基酸；與人endozepine胺基酸比較有77.1％相同。3’端非蛋白質轉譯區有207個核?酸。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:18:00Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . . . . . . . . . . . 1 前言. . . . . . . . . . . . . . . . . . . . 2 材料與方法. . . . . . . . . . . . . . . . . . . . 8 結果. . . . . . . . . . . . . . . . . . . . 21 討論. . . . . . . . . . . . . . . . . . . . 23 參考文獻. . . . . . . . . . . . . . . . . . . . 31 圖片. . . . . . . . . . . . . . . . . . . . 36 附錄. . . . . . . . . . . . . . . . . . . . 62 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯉魚視網膜受光蛋白基因的選殖、定序及分析 | zh_TW |
| dc.title | Some preliminary characteristics of the photoreceptor genes from a carp retina cDNA library | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 76 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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