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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Jing-Ing Lu | en |
| dc.contributor.author | 呂俊英 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:56Z | - |
| dc.date.available | 2021-07-01T08:17:56Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Breitbart, R. E., Nguyen, H. T., Medford, R. M., Destree, A. T., Mahdavi, V., and Nadal-Ginard, B. Intricate combinatorial patterns of exon splicing generate multiple regulated troponin T isoforms from a single gene. (1985) Cell 41, 67-82.
Briggs, M. M., and Schachat, F. N-terminal amino acid sequence of three functionally different troponin T isoforms from rabbit fast skeletal muscle. (1989) J. Mol. Biol. 206, 245-249. Bullard , B ., Dabrowskar , R ., and Winkelman , L . The contractile and regulatory proteins of insert flight muscle. (1981) Biochem. J. 135, 277-286. Chong, P. C. S., and Hodges, R. S. Proximity of sulfhydryl groups to the site of interaction between component of the troponin complex from rabbit skeletal muscle. (1982) J. Biol. Chem. (257), 2549-2555. Endo , T ., and Obinata , T . Troponin and its components from ascidian smooth muscle . J. Biochem . (1981) 89, 1599-1608 Garfinkel , L. I ., Periasamy , M ., and Nadal-Ginard , B . Cloning and haracterization of cDNA sequence corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, a-tropomyosin, and a-actin. (1982) J. Biol. Chem. 257, 11078-11086. Hastings, K. E. M., Bucher, E. A., and Emerson, C. P. Generation of troponin T isoforms by alternative RNA splicing in avian skeletal muscle. (1985) J. Biol. Chem. 260,13699-13703. Hill,R .W., and Wyse , G . A . Animal physiology. second edition . (1988) Harper & Row, Publishers , New York. Kavinsky, C. J., Umeda, P. K., Sinha, A. M., Elzinga, M., Tong, S.W., Zak, R., Jakovcic,S., and Rabinowitz, M. Cloned mRNA sequence for two types of embryonic myosin heavy chain from chicken skeletal muscle. (1983) J. Biol. Chem. 258, 5196-5205. Kobayashi, T., Takagi, T., Konishi, K., nd Coz, J. A. Amino acid sequence of crayfish troponin I. (1989a) J. Biol. Chem. 264, 1551-1557. Kobayashi , T ., Takagi T . Konishi, and Wnuk W . Amino acid sequence of crayfish troponin I. (1989b) 264, 18247-18259. Medford, R. M., Nguyen, H. T., DEstree, A. T., Summers, E., and Nadal-Ginard, B. A novel mechanism of alternative RNA splicing for the developmentally regulated generation of troponin T isoforms from a single gene. (1984) Cell 38, 409-421. Minty, A. J., Alonzo, S., Caravatti, M., and Buckingham, M. A fetal skeletal muscle actin mRNA in the mouse and its identity with cardiac actin mRNA. (1982) Cell 30, 185-192. Nishita, K. and Ojima T. American lobster troponin. (1990) J. Biochem. 108, 677-683. Ojima, T. and Nishita, K. Troponin from akazara scallop striated adducter muscle. (1986) J. Biol. Chem. 261, 16749-16754. Pearlstone, J. R., and Smillie, L. B. Effect of troponin-I plus -C on the binding of troponin-T and its fragment to a-tropomyosin. (1983) J. Biol. Chem. 258, 2534- 2545. Periasamy, M., Strehler, E. E., Garfinkel, L. I., Gubits, R. M., Ruiz-Opazo, N., and Nadal-Ginard, B. Fast skeletal muscle myosin light chain 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing. (1984) J. Biol. Chem. 159, 13595-13604. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular cloning: a laboratory manual. (1989) Cold Spring Harbor Laboratory Press, New York. Sanger, F., Nicklen, S., and Coulson, A. R. DNA sequence with chain terminating inhibitors. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467. Schachat, F. H., Bronson, D. D., and Mcdonald, O. B. Heterogeneity of contractile proteins. (1985) J. Biol. Chem. 260, 1108-1113. Schachat, F. H., Diamond, M. S., and Brandt, P. W. Effect of different troponin-T-tropomyosin combination on thin filamental activation. (1987) J. Mol. Biol. 198, 551-5 54. Shinoda , Y ., Yamada, A ., and Yagi , K . Identification of troponin-I of crayfish myofibrils . J. Biochem. 103, 636-640. Smillie, L . B ., Golosinska, K ., and Reinach , F . C. Sequence of complete cDNAs encoding four variants of chicken skeletal muscle troponin T . J . Biol . Chem.(1988) 263, 18816-18820. Tobacman, L. S., and Lee, R. Isolation and functional compari sion of bovin cardiac troponin T isoforms. (1987) J. Biol. Chem. 262, 4059-4064. Toyota, N., and Shimada, Y. Isoform variants of troponin in skeletal and cardiac muscle cells cultured with and without nerves. (1983) Cell 33, 297-304. Vander, A. J. Sherman, J. H. and Luciano , D . S. Human physiology, 3rd ed. McGraw-Hill , New York, (1980). Wnuk, W., Schoechlien, M., and Stein, E . A. Regulation of actomyosin ATPase by a single calcium- binding site on troponin C from crayfish. (1984) 259 ,9017-9023 Wydro, R. M.. Nguyen. H, T., Gubits. R. M.. and Nadal-Ginard. B. Characterization of sarcomeric myosin heavy chain genes. (1983) J. Biol. Chem. 258, 670-678. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76088 | - |
| dc.description.abstract | 選用rat troponin T exon-15 當 probe 從紅色吳郭魚骨骼肌建立的 cDNA library中，在同一 phage particle中，選殖出二段 cDNA 並加以定序，該 cDNA 片段分別有 2944 及 927 個核甘酸，前者包含5’端末轉譯區的 300 個核甘酸，轉譯區的 1830 個核甘酸，共可譯成 610 個胺基酸和3’端末轉譯區的 615 個核甘酸，此段 cDNA 與 rat TnT 比較，則有 67 ％的相似性， T1 轉譯成的胺基酸分子量約 68K daltons ，其 isoelectric point (PI) = 9 . 72 並具有 3 個 N－ glycosylation site 及 3 個 cAMP－and cGMP - dependent protein Kinase phosphorylation site ．後者包含5’端末轉譯區的 52 個核甘酸，轉譯區的 678 個核甘酸，共可轉譯成 226 個胺基酸及 3 ’端末轉譯區的 196 個核甘酸．而 T2所轉譯成的核甘酸分子量約 26K Daltons ，其 PI 值為 8.24 ，並具有3 個 protein Kinase C phosphsrylation site 。 最後以所得的cDNA insert 作 Northern blot ，分析是否具有組織專一性．在所取得的骨骼肌，平滑肌，心肌，肝，表皮，鰓等 6 個組織中，僅骨骼肌具有正反應，顯示所篩選到的基因為骨骼肌專一性基因。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:56Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . . . . . . . . . . . . . . . 1 前言. . . . . . . . . . . . . . . . . . . . . . . . . 2 材料與方法. . . . . . . . . . . . . . . . . . . . 5 一 材料. . . . . . . . . . . . . . . . . . . . . . . 5 二 方法. . . . . . . . . . . . . . . . . . . . . . . . 6 A cDNAlibrary的建立. . . . . . . . . . . . 6 B 篩選定序與比較. . . . . . . . . . . . . . 10 C 組織專一性分析. . . . . . . . . . . . . . 14 結果. . . . . . . . . . . . . . . . . . . . . . 15 一 cDNA library 的建立. . . . . . . . . 15 二 cDNA 的選殖與定序. . . . . . . . . . . . . . 15 三 核甘酸序列及轉譯成的胺基酸序列. . . . . . . 15 四 核甘酸序列及胺基酸序列之比較. . . . . . . . . . 16 a 核甘酸序列的比較. . . . . . . . . . . . . . . . . . b 胺基酸序列的比較. . . . . . . . . . . . . . . . . . 五 骨骼肌專一性分析. . . . . . . . . . . . . . . . . . 17 討論. . . . . . . . . . . . . 18 1 篩選探針的設計. . . . . . . . . . . . . . . . . . . . . 18 2 T1 T2 的方向性. . . . . . . . . . . . . . . . . . . . . 18 3 cDNA 序列的比較. . . . . . . . . . . . . . . . . . . . 18 4 胺基酸序列的比較. . . . . . . . . . . . . . . . . . . . .19 5 未來進行方向. . . . . . . . . . . . . . . . . 19 參考文獻. . . . . . . . . . . . . . . . . . .. . . 20 圖表. . . . . . . . . . . . . . . . . . .. . . . . . 22 附錄一. . . . . . . . . . . . . . . . . . . . . . . . 43 附錄二. . . . . . . . . . . . . . . . . . . . . 44 附圖一. . . . . . . . . . . . . . . . . . . . . . . . . 48 附圖二. . . . . . . . . . . . . . . . . . . . . . . . . 49 | |
| dc.language.iso | zh-TW | |
| dc.title | 紅色吳郭魚骨骼肌專一性基因的選殖、定序及比較 | zh_TW |
| dc.title | Cloning Sequencing and Comparison of Skeletal Muscle Specific Gene from Tilapia (Oreochrimos ,niloticus var.) | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 61 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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