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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳淑華 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:54Z | - |
| dc.date.available | 2021-07-01T08:17:54Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Burch, J. B. E., Evans, M. I., Friedman, T. M. and O’ Malley, P. J. 1988. Two functional estrogen response elements are located upstream of the major chicken vitellogenin gene. Mol. Cell Biol. 8:1123-1131.
Campbell, C M. and Idler, D. R. 1980. Characterization of an estradiol-induced protein from rainbow trout serum as vitellogenin by the composition and radioimmunological cross reactivity to ovarian yolk fractions. Biol. Reprod 22:605-617. Chan, S. L,, Tan, C. H., Pang, K. M. and Lam, T. M. 1991. Vitellogenin purification and development of assay for vitellogenin receptor in oocyte membranes of the tilapia (Oreochromis niloticus, L.) ．J. Exp. Zool.257:96 -109. Chang, C-F. and Chen, M-R. 1990. Fluctuation in sex steroids and sex steroid–binding protein during the development and annual cycle of the male common carp, Cyprinus carpio. Comp. Biochem. Physiol. 97A:565-568. Chang, C-F. and Lee, Y-H. 1992. Purification of the sex steroid-binding protein from common carp (Cyprinus carpio) plasma. Comp. Biochem. Physiol.101B:587-590. Chrismann, J. L., Greayson, M. J. and Hwang, R. C. C. 1977. Comparative study of hen yolk phosvitin and plasma vitellogenin. Biochemistry 16:3250-3256. Copeland, P. A., Sumpter, J. P. Walker, T. K. and Croft, M. 1986. Vitellogenin levels in male and female rainbow trout (Salmo gardneri Richardson) at various stages of the reproductive cycle. Comp. Biochem. Physiol. 83B:487-493. Covens, M., Stynen D., Ollevier, F. and de Loof, A. 1988. Concanavalin A reactivity of vitellogenin and yolk proteins of the threespined stickleback Gasterosteus aculeatus (Teleosti). Comp. Biochem. Physiol 90B:227-233. Crim, L. W. and Idler, D ．R. 1978. Plasma gonadotropin, estradiol, vitellogenin and gonad phosvitin levels in relation to the seasonal reproductive cycle of female brown trout. Ann. Biol. Anim. Biochem. Biophysiol 18:1001-1005. De Vlaming, V. L., Wiley, H. S., Delahunty, G. and Wallace, R. A. 1980. Goldfish (Carassius auratus) vitellogenin:induction, isolation, properties and relationship ot yolk proteins. Comp. Biochem. Physiol. 67 B:613-623. Felden, F., Leheup, B., Fremont, S., Bougueme, R. Egloff, M., Nicolas, J. P., Grignon, G. and Gueant, J. L. 1992. The plasma membrane of epididymal cells has a specigic receptor which binds to androgen-binding protein and sex steroid binding protein. J Steroid Biochem. Mol. Biol. 42:279-285. Foucher, J-L., Niu, P. D., Mourot, B., Vaillant, C. and le Gac, F. 1991. In vivo and in vitro studies on sex steroid binding protein (SBP) regulation in rainbow trout (Oncorhynchus mykiss): influence of sex steroid hormones and of factors linked to growth and metabolism. J. Steroid Biochem ．Mol. Biol. 39:975-986. Frairia, R., Fortunati, N., Berta, L., Fazzari, A., Fissore, F. and Gaidano, G. 1991. Sex steroid binding Protein (SBP) receptors in estrogen sensitive tissues. J. Steroid Biochem. Mol. Biol. 40:805-812. Gershagen. S., Fernlund, P. and Edenbrandt, C-M. 1991. The genes for SHBG / ABP and the SHBG–like region of vitamin K-dependent protein S have evolved from a common ancestral gene. J. Steroid Biochem．Mol. Biol 40:763-769. Goedake, A. and Berbroom, B. L. 1974. Studies on the maturation and fecundity of the pike, Esox lucius. Aquaculture 4:3-12. Green, C. D. and Tata, J. R. 1976. Direct induction by estradiol of vitellogenin synthesis in organ cultures of male Xenopus laevis liver. Cell7:131-139. Hamazaki, T. S., Iuchi, I. and Yamagami, K. 1987. Production of a “ spawning female-specefic substance ” in hepatic cells and its accumulation in the asscites of the estrogen-treated adult fish, Oryzias latipes，J. Exp. Zool. 242:325-332. Hara, A., Takano, K. and Hirai, H. 1983. Immunochemical identification of female-specigic serum protein, vitellogenin, in the medaka, Oryzias latipes (teleosts). Comp. Biochem. Physiol. 76A:135-141. Hara, A. and Hirai, H. 1978. Comparative studies on immunochemical properties of female-specfic serum protein and egg yolk protein in rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 59B:339-343. Hara, A., Yamauchi, K. and Hira. H. 1980. Studies on female-specific serum protein (vitellogenin) and egg yolk protein in Japanese eel (Anguilla japonica). Comp. Biochem. Physiol. 81B:275-279. Ho, S-M., Lance, L. and Megaloudis, M. 1987. Plasma sex-steroid binding protein in a seasonally breeding reptile, Alligator mississippiensis. Gen. Comp．Endocrinol. 65:121-132. Idler, D. R. and Campbell, C. M. 1980. Gonadotropin stimulation of estrogen and yolk procursor synthesis in juvenile rainbow trout. Gen. Comp. Endocrinol. 41:384-391. Kanungo, J., Petrino, T. R. and Wallace, R. A. 1990. Oogenesis in Fundulus heteroclitus. Ⅵ. Establishment and verification of conditions for vitellogenin incorporation by oocytes in vitro. J. Exp. Zool. 254:313-321. Klein-Hitpass, L., Tsai, S. Y., Greene, G. L., Clark, J. H., Tsai, M-J. and O’Malley, B. W. 1989. Specific binding of estrogen receptor to the estrogen responsive element. Mol. Cell. Biol. 9:43-49. Kumar, V. and Chambon, P. 1988. The estrogen receptor binds tightly to its responsive element as a ligend-induced homodimer. Cell 55:145-156. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685. Laurell, C. B. 1966. Quantitative estimation of protein by electrophoresis in agarose containing antibodies. Anal. Biochem. 15:45-52. Lazier, C. B., Lonergan, K. and Mommsen, T. P. 1985. Hepatic estrogen receptors and plasma estrogen-binding activity in the atlantic salmon. Gen. Comp. Endocrinol. 57:234-245. Lee, K. B. H., Lim, E. H., Lam, T. J. and Ding, J. L. 1992. Vitellogenin diversity in the perciformes. J. Exp. Zool. 264:100-106. Losso, J. N., Bogumil, R. and Nakai, S. 1993. Comparative studies of phosvitin form chicken and salmon egg yolk. Comp. Biochem. Physiol, l06B:919-923. Mackey, M. E. and Lazier, C. B. 1993. Estrogen responsiveness of vitellogenin gene expression in rainbow trout (Oncorhynchus mykiss) kept at different temperatures. Gen, Comp. Physiol. 89:255-266. Maitre, J. L., le Guellec, C., Derrien, S., Tenniswood, M. and Valotaire, Yves. 1984. Measurement of vitellogenin from rainbow trout by rocket immuno-electrophoresis: application to the kinetic analysis of estrogen stimulation in the male. Can J Biochem. Cell Biol. 63:982-987. Martin, B. and Xavier, F. 1981. High-affinity binding of progesterone, estradiol-17β and testosterone by plasma proteins of the reptile Lacerta vivipara J. Gen. Comp. Endocrinol. 43:299-307. Mercier-Bodard, C., Radanyi, C., Roux, C., Groyer, M. T., Robel, P., Dadoune, J.P., Petra, P. H., Jolly, D. J. and Baulieu, E. E., 1987. Cellular distribution and hormonal regulation of hSBP in human hepatoma cells. J Steroid Biochem. 27:297-307. Moore, F. L., Spielvogel, S. P., Zoeller ，R. T. and Wingfield, J. 1983. Testosterone-binding protein in a seasonally breeding amphibian. Gen. Comp. Endocrinol. 49:15-21. Nagler, J. J., Ruby, S. M., Idler, D. R. and So. Y. P. 1986. Serum phosphoprotein phosphorus and calcium levels as reproductive indicators of vitellogenin in highly vitellogenic mature female and estradiol-injected immature rainbow trout (Salmo gairdneri). Can. J.Zool. 65:2421-2425. Nath, P. and Sundararaj, I. S. 1981. Isolation and identification of female-specific serum lipophosphoprotein (vitellogenin) in the catfish, Heteropneustes fossilis (Bloch). Gen, Comp. Endocrinol. 43:184-190. Norberg, B., Bjornsson, B. T., Brown, C. L., Wichardt, U-P., Deftos, J, and Haux, C. 1989. Changes in plasma vitellogenin, sex steroids, calcitonin and thyroid hormones related to sexual maturation in female brown trout (Salmo trutta). Gen. Comp ．Endocrinol. 75:316-326. Olin, T., Westman, A. and von der Decken, A. 1989. Time- and temperature dependent appearance of vitellogenin in golgi vesicles and serum after estrogen treatment of salmon (Salmo salar). J. Exp. Zool. 249:213-220. Opresko, L. K. and Wiley H. S. 1987. Receptor-mediated endocytosis in Xenopus oocytes. I. Characterization of the vitellogenin receptor system. & Ⅱ. Evidence for two novel mechanisms of hormonal regulation. J Biol. Chem. 4109-4123. Pakdel, F., Feon, S., le Gac, F., le Menn, F. and Valotaire, Y. 1991. In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout. Mol. Cell. Endocrinol. 75:205-212. Perez, L. E., Fenton, M. I. and Callard, I. P. 1991. Vitellogenin-homologous of mammalian apoproteins. Comp. Biochem. Physiol. 100B:821-826. Petra. P. H., Namkung, P. C., Senear, D. F., McCrae, D. A., Rousslang, K. W., Teller, D. C. and Ross, J. B. A. 1986. Molecular characterization of the sex steroid-binding protein (SBP) of plasma. Re-examination of rabbit SBP and comparison with the human. madaque and baboon proteins. J Steroid Biochem. 25:191-200. Petra, P. H. 1991. The plasma sex steroid binding protein (SBP or SHBG). A critical review of resent developments on the structure, molecular biology and function．J. Steroid Biochem. Mol. Biol. 40:735-753. Pottinger, T. G. 1988. Seasonal variation in specific plasma-and target-tissue binding of androgens, relative to plasma steroid levels, in the brown trout, Salmo trutta L. Gen, Comp. Endocrinol. 70:334-344. Pugeat, M., Crave, J. C., Elmidani, M., Nicolas, M. H., Garoscio-Cholet, M., Dechaud. H. and Tourniaire, J. 1991. Pathophysiology of sex hormone binding globulin (SHBG):relation to insulin. J Steroid Biochem. MoL Biol. 40:841-849. Rabelo, E. M. and Tata. J. R. 1993 ．Thyroid hormone potentiates estrogen activation of vitellogenin genes and autoinduction of estrogen receptor in adult Xenopus hepatocytes. Mol. Cell. Endocrinol. 96:37-44. Redshaw, M. R. and Follett, B. K. 1971. The crystaline yolk-platelet proteins and their soluble plasma precursor in an amphibian Xenopus laevis. Biochem. J 124:759-766. Riley, D., Kleis-San Francisco, S. M. and Callard, I. P. 1988. A plasma steroid hormone binding protein in the viviparous water snake, Nerodia. Gen. Comp. Endocrinol. 71:419-428. Rosner, W., Hryb, D. J., Khan, M. S., Nakhla, A. M. and Romas, N. A. 1991. Sex hormone-binding globulin:anatomy and physiology of a new regulatory system. J Steroid Biochem．Mol. Biol. 40:813-820. Selman, K. and Wallance, R. A. 1982. Oocyte growth in the sheephead minnow: uptake of exogenous proteins by vitellogenic oocytes. Tissue & Cell 14:555-571. Shapiro, D. 1982. Steroid hormone regulation of vitellogenin gene expression. CRC Crit. Rev. Biochem. Silversand, C., Hyllner, S. J. and Haux, C. 1993. Isolation immunochemical detection, and observations of the instability of vitellogenin from four teleosts. J Exp. Zool. 267:587-597. So, Y. P., Idler, D. R and Hwang, S. I. 1985. Plasma vitellogenin in landlocked atlantic salmon (Salmo salar Ouananiche):isolation, homologous radioimmunoassau and immunological cross-reactivity with vitellogeini form other teleosts. Comp. Biochem. Physiol. 81 B:63-71. Speith, J., Nettleton, M., Zucker-Aprison, E., Lea, K. and Blumenthal, T. 1991. Vitellogenin motifs conserved in nemetodes and vertebrates. J Mol. Evol. 32:429-438. Steyrer, E., Barber, D. L. and Schreider, W. L. 1990. Evolution of lipoprotein and vitellogenin receptor binds the mammalian apolipoprotein E. J. Biol Chem. 265:19575-19581. Truss, M. and Beato, M. 1993. Steroid hormine receptors:interaction with deoxyribonucleic acid and transcription factors. Endocrine Rev. 14:459-479. Tyler, C. R. and Sumpter, J. P. 1990. The purification and partial characterizationo of carp， Cyprinus carpio, vitellogenin. Fish Physiol. Biochem. 8:111-120. Tyler, C. R., Sumpter, J. P., Kawauchi, H. and Swanson, P. 1991. Involvement of gonadotropin in the uptake of vitellogenin into vitellogenic oocytes of the rainbow trout, Oncorhynchus mykiss. Gen Comp Endocrinol 84:291-299. Tyler, C. 1993. Electrophoretic patterns of yolk proteins throughout ovarian development and their relationship to vitellogenin in the rainbow trout, Oncorhynchus mykiss. Comp. Biochem. Physiol. 106B:321-329. Tyler, C. R,, Sumpter, J. P. and Bromage, N. R. 1988. In vivo ovarian uptake and processing of vitellogenin in the rainbow trout, Salmo gairdneri. J Exp. Zool. 246:171-179. Varriale, B., Pierantoni, R., Matteo, L. D., Minucci, S.，Milone, M. and Chieffi, G. 1988. Relationship between estradiol-17 β seasonal porfile and annual vitellogenin content of liver, fat body, plasma, and ovary in the frog (Rana esculenta). Gen. Comp. Endocrinol. 69:328-334. Waagboe, R. and Sandnes, K. 1988. Determination of vitellogenin in serum of rrainbow trout (Salmo gairdneri) by high-performance gel chromatography．J. Chromatogrqph. 427:138-143. Wahli, W., David, I. B., Ryffel, G. U. and Weber, R. 1981. Vitellogenesis and vitellogenin gene family. Science 212:298-304. Wahli, W. 1988. Evolution and expression of vitellogenin genes ．Trends Genet. 4:227-232. Waters, S. and von der Decken, A. 1992. Tissue-specific sensitivity of chromatin and the vitellogenin gene to micrococcal nuclease afetr continous exposure of salmon (Salmo salar) to 17 β- estradiol. Gen. Comp. Endocrinol. 87:105-110. Wiley, H. S., Opresko, L. and Wallance, R. A. 1979. New methods for the purification of vertebrate vitellongenin. Anal. Biochem. 97:142-152. Yaron, Z. and Barto, C. 1980. Stimulation of estradiol-17 β output from isolated ovarian fragments of the plaice (Pleuronectes platessa) by homologous pituitary extract. Gen. Comp. Endocrinol: 42:151-154. Yoshikuni, M. and Nagahama, Y. 1991. Endocrine regulation fo gametogenesis in fish. Bull Inst. Zool., Acad Sinica, Monograph 16:139-172. 張百恩， 1992。香魚血漿中卵黃前質及雌二醇之定量及其特性之研究。碩士論文。碩士論文。 鄭恆仲， 1993。白鰻催熟效果之比較。碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76085 | - |
| dc.description.abstract | 雌性卵生脊椎動物進入生殖週期之初期，在雌二醇誘導下，肝細胞進行卵黃前質合成，經由血液運送至卵巢，以胞飲作用進入卵細胞中，進一步分解為磷卵黃（phosvitin）及脂卵黃（ lipovitellin)，為堆積於卵細胞中之主要卵黃蛋白，此生理現象為卵細胞成熟之重要特徵。本論文擬就血液雌二醇結合蛋白、卵黃前質鑑定及特性、以及雌二醇誘導卵黃前質生成作用等進行研究，期能針對與卵黃生成作用相關生理獲得更進一步之瞭解。 以 HPLC-陰離子交換管柱層析分析方法，比較雌二醇注射處理性未成熟烏魚血清、雄魚血清與成熟中雌魚血清，鑑定雌性特有、雌二醇誘發之強陰電性蛋白質吸收峰，並比較此吸收峰與卵黃蛋白萃取液之胺基酸組成及免疫性質，確認其為卵黃前質蛋白。以 7% Native-PAGE 配合以各種特性染色分析，確定卵黃前質具有醣蛋白、脂蛋白及磷酸化特性，且其蛋白質相當不穩定。經由 10% SDS-PAGE 分析，可知卵黃前質蛋白主要由五條太鏈所組成，其大小均接近於 116kd。 以不同劑量之雌二醇注射處理性未成熟魚，約可誘導卵黃前質生成；，則血清中卵黃前質最高含量之升高與注射劑量呈相關性。以 12.5 ug/g 體重雌二醇劑量注射尚未進入生殖季之成魚，其血清中之卵黃前質含量在第十天可達 121.73 士 33.73 mg/ml，遠高於生殖季內成熟烏魚採樣測值（0-2.6 mg/ml), 其可能原因為卵黃前質在非生殖季中無法進入卵細胞，而造成高量蓄積於血液中，或因雌二醇注射劑量高於一般之生理值，而造成之藥理現象。 烏魚血清中，證實有與雌二醇具專一性結合能力蛋白之存在。以不同之類固醇進行競爭性結合，發現其與一般性類固醇結合力高，但與助孕素之結合能力不佳；經以 HPLC-陰離子交換管柱分離雌二醇結合蛋白並進行 7 % Native-PAGE 分析，發現雌二醇放射性集中於 Rf 值 0.43 處，但可能因蛋白質含量過低，而未能對應於一電泳染色帶。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 摘 要………………………………………………………………………………………………i
前 言…………………………………………………………………………………………… l 一、魚類卵巢發育及卵黃前質合成過程…………………………………………………………2 二、性類固醇結合蛋白之特性……………………………………………………………………5 材料與方法…………………………………………………………………………………………8 一、材料來源及處理………………………………………………………………………………8 二、高效液相管柱層析法（High Performance Liquid Chromatography )……………………9 三、聚丙烯醞銨膠體電泳（ Polyacrylamide Gel Electrophoresis )……………………………10 （一）自然聚丙烯醞銨膠體電泳（ Native Polvacrvlamide gel electrophoresis )…………10 （二） SDS - 聚丙烯醯銨膠體電泳（SDS-Polyacrylamide gelelectrophoresis,SDS-PAGE)……10 （三）電泳膠體染色方法…………………………………………………………………………11 四、胺基酸組成分析（ Amino Acid Composition Analysis )…………………………………15 五、抗血清之製備及卵黃前質免疫分析…………………………………………………………16 （一）抗血清之製備過程………………………………………………………………………6 （二）火箭式免疫電泳（ Rocket immunoelectorphoresis )……………………………………17 六、性類固醇結合蛋白分析………………………………………………………………………18 （一）雌二醇結合實驗……………………………………………………………………………18 （二）雌二醇與性類固醇之結合平衡…………………………………………………………18 （三）性類固醇結合蛋白之初步純化及鑑定……………………………………………………19 七、蛋白質之測定…………………………………………………………………………………20 結 果……………………………………………………………………………………………21 一、卵黃前質之鑑定及特性分析………………………………………………………………21 （一）卵黃前質之鑑定與 HPLC 分析…………………………………………………………22 （二）卵黃前質之非變性膠體電泳形式及染色性質…………………………………………23 （三）卵黃前質之 SDS-PAGE 分析…………………………………………………………23 （四）卵黃前質與卵黃蛋白之胺基酸組成分析…………………………………………………24 （五）卵黃前質之免疫特性分析…………………………………………………………………24 二、卵黃前質之定量結果…………………………………………………………………………25 （一）卵黃前質之定量分析：火箭式免疫電泳法………………………………………………25 （二）幼魚施以雌二醇注射處理對血液中卵黃前質之合成影響………………………………26 （三）性腺未發育成魚施以雌二醇注射處理對血液中卵黃前質之含量影響…………………26 （四）卵黃生成期內雌性烏魚血液中卵黃前質之合成量……………………………………27 三、性類固醇結合蛋白之鑑定與特性探討………………………………………………………27 （一）血液中之雌二醇專一性結合蛋白之鑑定…………………………………………………27 （二）性類固醇結合蛋白與雌二醇間之結合平衡…………………………………………27 （三）性類固醇及其他類固醇對雌二醇一結合蛋白平衡態之競爭能力………………………28 （四）性類固醇結合蛋白之初步分離……………………………………………………………32 討 論……………………………………………………………………………………………29 一、烏魚卵黃前質之鑑定及特性分析……………………………………………………………29 二、烏魚卵黃前質與卵黃萃取液之比較…………………………………………………………33 三、卵黃前質之定量方法…………………………………………………………………………34 四、雌二醇對卵黃前質合成之影響………………………………………………………………35 五、血液中性類固醇結合蛋白之鑑定及其特性探討……………………………………………36 參考文獻…………………………………………………………………………………………38 圖 表…………………………………………………………………………………………47 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯔魚卵黃前質及性類固醇結合蛋白之特性分析 | zh_TW |
| dc.title | IDENTIFICATION AND CHARACTERIZATION ON VITELLOGENIN AND SERUM SEX STEROID-BINDING PROTEIN OF GREY MULLET (Mugil cephalus L.) | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 85 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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