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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 方玫貞 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:53Z | - |
| dc.date.available | 2021-07-01T08:17:53Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Ahn, K. Y., Madsen, K. M., Tishen, C. C., Kone, B. C. (1993). Differential expression and cellular distribution of mRNA encoding α and β isoforms of Na, K-ATPase in rat Kidney. Am. J. Physiol., 265, F792-F801.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76083 | - |
| dc.description.abstract | 本研究探討廣鹽性吳郭魚（ Oreochromis mossambicus ）鰓是否表現不同型Na, K-ATPase α subunit 基因，以及這些基因是否分別在海水或淡水環境中表現不同的功能。 以 RT-PCR 方法找到吳郭魚至少具有 5 種不同型 Na , K-ATPase α subunit 基因。本實驗確定 TG3 (α1）、 TH23 、 TH6 、 S34-2 、 S34-9 是 Na , K-ATPase α subunit isoform 基因之證據包括: 1、在 GCG bank 作比對，氨基酸相似性最高的前 20 個蛋白質都是 Na , K-ATPase α subunit ，有 75 ％-84 ％以上相似性。 2、Na , K-ATPase α subunit 的 active site 氨基酸都相同 3、除 TH6 外，以其餘 4 種作 DNA 探針，進行各個組織 RNA 北方式點墨法，都具有 Na , K-ATPase α subunit 相近位置。 此外以 RT-PCR 方法得到一個較大的吳郭魚 Na , K-ATPase α subuni t DNA 片段，長約 1685bp ，命名為 OM α1，可轉譯為一個含 565 個氨基酸的蛋白質，進入 GCG bank 作氨基酸相似性比對，皆與已知動物 Na , K-ATPase α1 subunit 有 85 % 以上的相似性，而且 active site 的氨基酸都相同，因此推測OMαl 是屬於哺乳類 Na , K-ATPase α1 subunit 的相似基因。 本實驗以 2 種方法證明不論是海水或淡水鰓都具有不同型 Na , K-ATPase α subunit 基因表現。海水魚鰓與淡水魚鰓都具有 TG3 (α1）這型 Na, K-ATPase α subunit 而且基因表現量較其他 isoform 高。在 TG3 (α1) isoform，海水魚鰓的基因表現量高於淡水魚鰓。在 TH23 isoform，海水魚鰓基因表現量非常少；淡水魚鰓相對海水魚而言，基因表現量較高。至於 S34-2與S34-9 isoform 基因只表現在淡水魚鰓。淡水魚鰓比海水魚鰓表現較多型 Na , K-ATPase α subunit , 可以解釋淡水魚鰓和海水魚鰓完全不一樣的功能機制。 本實驗是第一次直接證明廣鹽性吳郭魚鰓有不同型 Na , K-ATPase α subunit 基因表現，而且不論是海水或淡水吳郭魚鰓表現最多的 Na , K-ATPase α subunit是α1。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:53Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 摘要……………………………………………………………………………………………2
壹、前言………………………………………………………………………………………3 貳、材料與方法………………………………………………………………………………6 （1）材料……………………………………………………………………………………6 （2）實驗內容………………………………………………………………………………6 （3）實驗方法………………………………………………………………………………7 參、結果………………………………………………………………………………………16 （1） Na, K-ATPase α subunit cDNA 片斷……………………………………………l6 （2） Na, K-ATPase α sununit 部份 cDNA sequence…………………………………l6 （3） 吳郭魚基因體 DNA ( genomic DNA ）的 Southern blot………………………l7 （4） Na, K-ATPase α subunit isoform probe專一性…………………………………17 （5） 淡水與海水吳郭魚鰓 Na, K-ATPase α subunit 基因表現……………………l8 （6） 吳郭魚不同組織器官 Na, K-ATPase α subunit isoform 基因表現 …………l9 （7） 不同鹽度環境，對鰓與腎臟 Na , K-ATPase α subunit 基因表現的影響 ……20 （8） 吳郭魚腎臟及腦的 cDNA library （基因庫）之篩選基因 …………………20 肆、討論………………………………………………………………………………………21 伍、附錄………………………………………………………………………………………26 陸、參考資料…………………………………………………………………………………30 柒、圖表 | |
| dc.language.iso | zh-TW | |
| dc.title | 吳郭魚鰓不同型 Na , K – ATPase α 次單原基因之研究 | zh_TW |
| dc.title | Study on gene of different isoforms of Na , K - ATPase α subunit in the gills of tilapia （ Oreochomis mossambicus ) | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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