請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76081完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳瑜亮 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:52Z | - |
| dc.date.available | 2021-07-01T08:17:52Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Andreason, G. L. and Evans, G. A. (1989) Optimization of electroporation for transfection of mammalian cell lines. Anal. Biochem. 180:269-275. Arezzo, F. (1989) Sea urchin sperm as a vector of foreign genetic information. Cell Biol. Intl. Rep. 13:391-403. Brem, G. ，Brening, B. ， Horstgen-Schear, G. and Winnacker, E. L. (1988) Gene transfer in tilapia (Oreochromis niloticus). Aquaculture 68:209-219. Billard, R. and Cossom, M. P. (1992) Some problems related to the assessment of sperm motility in freshwater fish. J. Exp. Zool.261:122-131 Brackett, B. G., Baranska, W., Sawicki, W. and Koprowski, H. (1971) Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc. Natl. Acad. Sci. U. S. A 68 (2):353-357. Brinster, R. L., Snadgren, E. P., Behringer, R. R. and Palmiter, R. D. (1989) No simple solution for making transgenic mice. Cell 59:239-241. Birnstiel, M. and Busslinger, M. (1989) Dangerous liaison: Spermatoza as natural vectors for foreign DNA Cell 57:701-702. Chong, S. S. C. and Vielkind, J. R. (1989) Expression and fate of CAT reporter gene microinjected into fertilized medaka(Oryzias latipes) eggs in the form of plasmid DNA, recombinant phage particles and its DNA. Theor. Appyl. Genet.78:369-380. Culp, P., Christiane, N. V. and Hopkins, N. (1991) High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc. Natl. Acad. Sci. USA 88:7953-7957. Chourrout, D. and Perrot, E. (1992) No transgenic rainbowtrout produced with sperm incubated with linear DNA. Mol. Mar. Biol. Biotech. 1:282-285. Chourrout, D., Guyomard, R. and Houdebine, L. M. (1986) High efficiency gene transfer in rainbow trout (Salmogairdneri rich.) by microinjection into egg cytoplasm. Aquaculture 51:143-150. Du, S. J., Gong, Z., Fletcher, G. L, Shears, M. A., King, M. J., Idler, D. R. and Hew, C. L. (1992) Growth enhancement in transgenic Atlantic salmon by the use of an all fish chimeric grouth hormone gene construct. Bio / Tecnology 10:176-181. Du, S. J., Gong, Z., Hew, C. L., Tan, C. H. and Fletcher, G. L. (1992) Development of an all-fish cassete for gene transfer in aquaculture. Mol. Mar. Biol. Biotech. 1:290-300. Dunham, R. A., Ramboux, A. C., Duncan, P. L., Chen, T. T., Lin, C. M., Kight,. K., Gonzalez-Villasenor, I. and Powers, D. A. (1992) Transfer, expression, and inheritance of salmonid growth hormone genes in channel catfish, (Ictalurus punctatus,) and effects on performance traits. Mol. Mar. Biol. Biotech. l:380-389. Erickson, R. P. (1990) Are intermediate vectors needed between foreign DNA on sperm and the nucleus Trends in Genetic 6(2)31. Fletcher, G. L. and Davies, P. L. (1991) Transgenic fish for aquaculture. Genet. Engineer. 13:331-370. Graham, L., Van, A. J. (1973) A new method for the assay of infectivity of human adenovirus 5 DNA. Virol., 52:456-467. Guyomard, R., Chourrout, D., Leroux, C., Houdebine, L. M. and Pourrain, F. (1989) Integration and germ line transmission of foreign genes microinjected into fertilized trout eggs. Biochimie 71:857-863. Ginsburg, A. S. (1963) Sperm-egg association and its relationships to the activation of the eggs in salmonid fishes. J. Embryol. Exp. Morph. 11:13-33. Gagne, M. B., Pothier, F. and Sirard, M. A. (1991) Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. Mol. Reprod. Dev. 29:6-15. Gavora, J. S., Benkel, B., Sasada, H., Cantwell, W. J., Fiser, P., Nagai, J. and Sabour, M. P. (1991) An attemp at sperm-mediated gene transfer in mice and chickens. Can. J. Anim. Sci. 71:287-291. Gong, Z. G., Hew, C. L. and Vielkind, J. R. (1911) Functional analysis and temporal expression of promoter regions from fish antifreeze protein genes in transgeic Japanese medaka embryos. Mol. Mar. Biol. Biotech. 1:64-72. Gandolfi, G., Lavitrano, M., Camaioni, A., Spadafora, C., Siracusa, G. and Lauria, A. (1989) The use of sperm–mediated gene transfer for the generation of transgenic pigs. J. Repord. Fertil. Abstr. Ser. 4:10. Hew, C. L. (1989) Transgenic fish:present status and future directions. Fish Physiol. and Biochem. 7:409-413. Houdebine, L. M. and Chourrout, D. (1991) Transgenesis in fish. Experientia 47:891-897. Inoue, K., Yamashita, S., Hata, J.I., Kabeno, S., Asada, s., Nagahisa, E. and Fujita, T. (1990) Electroporation as a new technique for producing transgenic fish. Cell Differ. Dev. 29:123-128. Kurokura, H.， Hirano, R., Tomita, M. Iwahashi, M. (1984) Cryopreservvation of carp sperm. Aquaculture 37:267-273 Khoo, H. W., Ang, L. H. and Wong, K. Y. (1992) Sperm cells as vectors for introducing foreign DNA into zebrafish. Aquaculture 107:1-19. Klenchin, V. A., Sukharev, S. I., Serov, S. M., Chernomordik, L. V. and Chizmadzhev, Y. A. (1991) Electrically induced DNA uptake by cells is a fast process involving DNA electroporesis. Biophys. J. 60:804-811. Knutson, J. C. and Yee, D. (1987) Electroporation: Parameters affecting transfer of DNA into mammalian cells. Anal. Biochem. 164:44-52. Lavitrano, M., Camaiono, A., Farace, M. G. and Spadafora, C. (1989) Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57:717-723. Lu, J. K., Chen, T. T., Chrisman, C. M., Andrisani， O. M. and Dixon, J. E. (1992) Integration, expression, and germ-line transmission of foreign growth hormone genes in medaka (Oryzias latipes) Mol. Mar. Biol. Biotech. 1:366-375. Mcevoy, T., Stack, M., Keane, B., Barry, T., Sreenan, J. and Gannon, F. (1988) The expression of a foreign gene in salmon embryos. Aquaculture 68:27-37. Maclean, N., Penman, D. and Zhu, Z. (1987) Introduction of novel genes into fish. Bio / Technology 5: 257-261. Muller, F., Ivics, Z., Erdelyi, F., Papp, T., Varadi, L., Horvath, L., Maclean, N. and Orban, L. (1992) Introducing foreign genes into fish eggs with electroporated sperm as a carrier. Mol. Mar. Biol. Biotech. 1:276-281. Ozato, K., Inoue, K. and Wakamatsu, Y. (1989) Transgenic fish: biological and technical problems. Zool. Sci. 6:445-457. Powers, D. A (1989) Fish as model system. Science 246:352-358. Powers, D. A., Hereford, L., Chen T. T., Lin, C. M., Kight, K., Creech, K. and Dunham, R. (1992) Electroporation: a method for transferring genes into the gamets of zebrafish (Brachydanio rerio), channel catfish (Ictalurus punctatus), and common carp (Cyprinus carpio) Mol. Mar. Biol. Biotech. 1:301-308. Perez, A., Solano, R., Castro, F. O., Leonart, R., Armaas, R. D., Martinez, R., Aguilar, A., Herrera, L. and Fuente, J. D. L. (1991) Sperm cell mediated gene transfer in cattle. Biotechnol. Appl. 8:90-94. Rols, M. P. and Teissie, J. (1990) Electropermeabilization of mammalian cells quantitative analysis of the phenomenon. Biophys. J. 58:1089-1098. Stuart, G. W., Mcmurray, J. V. and Westerfield, M. (1988) Replication, intergration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development 103:403-412. Shigekawa, K. and Dower, W. J. (1988) Electroporation of eukaryotes and prokaryotes: A general approach to the introduction of macromolecules into cells. Biotechniques6 (8):742-751. 43. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular cloning:a laboratory mannual (Cold Spring Harbor, New York) Sin, F., Bartly, A., Walker, S., Bulman, S., Allison, L. A., Hopkin, C. L. and Sin, I. (1990) Electroporation of fish sperm for gene transfer. Cell Biol. Int. Rep. 14:167. Sendai, Y. and Aketa, K. (1991) Activation of Ca transport system of Sea Urchin sperm by high external pH: 220kD membrane glycoprotein is involved in the regulation of the Ca entry. Develop. Growth. & Differ. 33 (2) 101-109. Symonds, J. E., Walker, S. P. and Sin, F. Y. T. (1994) Electroporation of salmon sperm with plasmid DNA: evidence of enhanced sperm/DNA association. Aquaculture 119:313-327. William, R. S., Jonston, S. A. ，Riedy, M., Devit, M. J., Mcelligott, S. G. and Sndford, J. C. (1991) Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proc. Natl. Acad. Sci. USA 88: 2726-2730. Wolf, H., Rols, M. P., Boldt, E., Neumann, E. and Teissie, J. (1994) Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophys. J. 66:524-531. Winterbourne, D. J., Thomas, S., Hermon-Taylor, J., Hussain, I. and Johnstone A. P. (1988) Electric shock-mediated transfection of cells. characterization and optimization of electrical parameters. Biochem. J. 251:427-434. Wong, T. K. and Neumann, E. (1982) Electric field mediated gene transfer. Biochem. Biophys. Res. Comm. 107 (2) 584-587. Winkler, C., Vielkind, J. R. and Schartl, M. (1991) Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes). Mol. Gen. Genet. 226:129-140. Witkin, S. S. (1989) Can sperm capture foreign DNA for expression in the embryo Fertil. and Steril. 52 (4) 692. Xie, T. D. and Tsong, T. Y. (1992) Study of the mechanisms of electric field-induced DNA transfection. Ⅲ. electric parameters and other conditions for effective transfection. Biophys. J. 63:28-34. Xie, T. D., Sun, L., Zhao, H. G., Fuchs, J. A. and Tsong, T. Y, (1992) Study of mechanisms of electric field-induced DNA transfection. IV.. efects of DNA topology on cell uptake and transfection efficiency. Biophys. J. 63:1026-1031. Xie, T. D. and Tsong, T. Y. (1993) Study of mechanisms of electric field–induced DNA transfection. V. effects of DNA topology on surface binding, cell uptake, expression, and intergration into host chromosome of DNA in the mammalian cell. Biophys. J. 65:1684-1689. Xie, Y., Liu, D., Zou, J., Li, G. and Zhu, Z. (1993) Gene transfer via electroporation in fish. Aquaculture 111: 207-213. Zhang, P., Hayat, M., Joyce, C., Gonzalez, L. I., Lin, C. M., Dunham, R. A., Chen, T. T. and Powers, D. A. (1990) Gene transfer, expression and inheritance of pRSV-rainbowtrout-GH cDNA in the common carp, (Cyprinus carpio linnaeus). Mol. Reprod. Dev. 25:3-13. Zelenin, A. V., Alimov, A. A., Barmintzev, V. A., Beniumov, A. O., Zelenina, I. A., Kolesnov, A. M. and Kolesnikov, V. A. (1991) The delivery of foreign genes into fertilized eggs using high-velocity microprojectiles. FEBS Lett. 287:118-120. Zhao, X., Zhang, P. J. and Wong, T, K. (1993) Application of baekonization: a new approach to produce transgenic fish. Mol. Mar. Biol. Biotech. 2:63-69. Zhu, Z., Li, G., He, L. and Chen, S. (1985) Novel gene transfer into the fertilized eggs of gold fish (Carassius auratus) Z. Appl. Ichthyol. l:31-34. 曾福生（ 1993 ）以電穿孔法促使精子攜帶外來基因之探討：泥鰍之基因轉殖，國立臺灣大學漁業科學研究所碩士論文． | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76081 | - |
| dc.description.abstract | 利用電破法作為魚類基因轉殖到受精卵或胚胎的方式已被廣泛使用，但為了達到快速，大量及高轉殖效率的目的，精子載體攜帶外來基因，不失為良好的方式．本研究中利用 3 種帶有不同基因構築的質體，經由電破法轉殖入泥鰍精子，並分析轉殖後小魚的染色體。 結果顯示， ( l ）電破溶液方面：在精子活力及孵化率上，生理食鹽水為佳，且較 PBS 林格氏液或加鈣溶液接近控制組． ( 2 ）電破使用的 DNA 型式：轉殖 circular 與 linear 型式 pOPAFP-2000CAT ，經 Dot blot 分析，以 circular ( 50 % ）高於 linear ( 20 % ）。（3）電壓對魚苗孵化率及畸形率的關係：電壓由 8KV 降到 3.5KV 以下，其孵化率及畸形率分別為 13 % , 20 % （8KV）； 28 % , 0.05 %（3.5KV）電壓與孵化率為負相關與畸形率為正相關性（ 4 ）電破條件與活存率關係：改變電壓或其他參數，對於電破處理後所得到 2 cell stage 胚胎比例之間並無顯著差異（5）DNA 的存在與孵化率的關係：在 8KV 下， DNA 的有無，其孵化率相差一倍 ；3.5KV 下，則無差別（6）DNA 濃度的影響：使用 pOP5MGH2175 μg/ml 轉殖率為 43.3 ％高於 100μg/ml組的 28.5 ％而 DNA 濃度高於 25μg/ml，其孵化率 15.3 % ，控制組為 28.6 % ，此濃度下孵化率較低且與其他各組有顯著差異。(7)轉殖不同分子量質體及 cycle 數的影響：使用 pUC19 質體，並從 8cycle增加到 12 cycle ，其轉殖率由 40 ％提昇到 60 % ( 8 ）電破過程對 DNA 結構及轉殖後 Southern blot 的分析：電破 circular DNA ，電泳分析上層液 DNA ，其移動位置集中在近 linear form 位置，沉澱回收後則回後 circular 型式。 Southern blot 分析電破後發育到 64 cell stage 胚胎，有以 extrachromosome 質體型式存在，綜合以上結果，以 3.5kv , NP = 2 , cycle = 12 , Tb = 1.6s , Tp = 120 μs , D = lmm 條件下，轉殖外來基因到精子，能得到 50 ％以上，大量且形態正常的轉殖魚。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 摘要 前 言………………………………………………………………………………………………l 材 料………………………………………………………………………………………………3 1、魚種…………………………………………………………………………………………3 2、質體…………………………………………………………………………………………3 3、儀器…………………………………………………………………………………………3 4、藥品及溶液配方…………………………………………………………………………4 方 法………………………………………………………………………………………………5 （Ⅰ）電破用DNA的製備…………………………………………………………………5 （Ⅱ）精子載體的製備及實驗設計…………………………………………………………6 （Ⅲ）統計上的分析…………………………………………………………………………8 （Ⅳ）泥鰍魚苗 genomic DNA 的抽取……………………………………………………8 （Ⅴ）DNA 點墨法（Dot blot）……………………………………………………………8 （Ⅵ）南方氏點墨法（ Southern blot )………………………………………………………8 結 果………………………………………………………………………………………………10 討 論………………………………………………………………………………………………13 參考文獻…………………………………………………………………………………………17 圖 表………………………………………………………………………………………………25 附錄一（藥品及廠商）……………………………………………………………………………42 附錄二（溶液液配方）……………………………………………………………………………43 附 圖………………………………………………………………………………………………46 | |
| dc.language.iso | zh-TW | |
| dc.title | 精子載體 探討以魚類精子作為傳遞外來基因的媒介 | zh_TW |
| dc.title | Sperm carrier Study on sperm-mediated gene transfer of fish | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 50 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。