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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林佳蓉 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:51Z | - |
| dc.date.available | 2021-07-01T08:17:51Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | 宋宏紅，1994，草蝦抗弧菌病的研究，國立臺灣大學動物學研究所博士論文， pp. 32-43. 沈明來，1993，第四章：處理均值比較測驗， pp. 49-68 。 第七章：隨機區集完全設計， pp. 99-120. ，試驗設計學，九州圖書文物有限公司，臺北。 Agerberth, B., Lee, J. Y., Bergman, T., Carlquist, M., Boman, H. G., Mutt, V. and Jornvall, H. 1991. Amino acid sequence of PR-39 isolation from pig intestine of a new member of the family of proline-arginine-rich antibacterial peptides. Eur. J. Biochem. 202: 849-854. Bevins, C. L. and Zasloff, M. 1990. Peptides from frog skin Annu. Rev. Biochem. 59: 395-414. Boman, H. G. 1991. Antibacterial peptides: key components needed in immunity. Cell. 65: 205-207. Boman, H. G., Faye, I., Gudmundsson, D. H., Lee, J. -Y. and Lidholm, D-A. 1991. Cell-free immunity in cecropia. Eur. J. Biochem. 201:23-31. Boman, H. G., Faye, I., Hofsten P. V., Kockum, K., Lee, J. Y. and Xanthopoulos, K. G. 1985. On the primary structures of lysozyme, cecropins and attacins from Hyalophora cecropia. Dev, Comp. Immunol. 9: 551-558. Boman, H. G. and Hultmark D. 1987. Cell-free immunity in insects, Ann. Rev. Microbiol. 41: 103-126. Brey, P. T., Lee, W.-J., Yamakawa. M., Koizumi， Y., Perrot, S., Francois, M. and Ashida, M. 1993. Role of the integument in insect immunity: Epicuticular abrasion and induciton of cecropin synthesis in cuticular epithelial cells. Proc. Natl. Acad. Sci. USA 90: 6275-6279. Chadwick, J. S. and Dunphy, G. B. 1986. Antibacterial and antiviral factors in arthropod hemolymph. pp. 287-330. In: Gupta, A. P.( editor ). Hemocytic and Humarol Immunity in Arthropods. Jonh Wiley & Sons, Inc. USA. Christensen, B., Fink, J., Merrifield, B. and Mauzerall, D. 1988. Channel-forming properties and related model compounds incorporated into planer lipid membranes. Proc, Nalt. Acad. Sci, USA 85: 5027-5076. Dickinson, L. Russell, V. and Dunn, P. E. 1988. A family of bacteria-regulated, cecropin D-like peptides from Manduca sexta. J. Biol. Chem. 263: 19424-19429. Durell, S. R. Raghunathan, G. and Guy, H. R. 1992. Modeling the ion channel structure of cecropin. Biophys. J. 63: 1623-1631. Dunn, P. E., Dai, W., Kanost, M. R. and Geng, C. 1985. Soluble peptidoglycan fragments stimulate antibacterial protein synthesis by fat body from larvae of Manduca sexta. Dev. Comp. Immunol. 9: 559-568. Evans, E. E., Painter, B., Evans, M. L. Weinheimer, P. and Acton, R. T. 1968. An induced bactericidin in the spiny lobster, Panulirus argus.Proc. Xoc. Exp. Biol. Med. 128: 394-398. Fenouil, E. and Roch, P. 1991. Evidence and characterization of lysozyme in six species of freshwater crayfishes form astacidae and cambaridae families. Comp. Biochem. Physiol, 99B: 43-49. Flyg, C., Dalhammar, G., Rasmuson, B. and Boman, H. G. 1987. Insect immunity: Inducible antibacterial activity in Drosophila. Insect Biochem. 17: 153-160. Gotz, P., Boman, A. and Boman, H. G. 1981. Interactions between insect immunity and an insect-pathogenic nematode with symbiotic bacteria. Proc. R. Soc. London Ser. B 212: 333-350. Hoffmann, D., Hultmark, D. and Boman, H. G. 1981. Insect immunity: Galleria mellonella and other lepidoptera have cecropia-P9-like factors active against gram negative bacteria. Insect Biochem. 11: 537-548. Holak, T. A., Engstrom. A., Kraulis, P. T., Lindeberg, G., Bennich, H., Jones, T. A., Gronenborn, A. M. and Clore, G. M. 1988. The solution conformation of the antibacterial peptide cecropin A: A nuclear magnetic resonance and cynamical stimulated annealing study. Biochemistry 27: 7620-7629. Hong, T-H., Chen, S-T., Tang, T-K., Wang, S-C. and Cheng, T. H 1989. The production of polyclonal and monoclonal antibodies in mice using novel immunization methods. J. Immunol. Meth. JIM 05183. Hultmark, D., Steiner, H., Rasmuson, T. and Boman, H. G. 1980. Insect immunity, Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. Eur. J. Biochem. 106: 7-16. Hultmark, D., Engstrom, A., Bennich, H., Rashmi K. and Boman, H. G. 1982. Insect immunity: Isolation and structure of cecropin D and four minor antibaterial components from cecropia pupae. Eur. J. Biochem. 127: 207-217. Hultmark, D. Engstrom, A. Andersson, K. Steiner, H. Bonnich, H. and Boman. H. G. 1983. Insect immunity, Attacins, a family of antibacterial proteins from Hyalophora cecropia. EMBO J. 2: 571-576. Hultmark, D. 1993. Immune reactions in Drosophila and other insects: A model for innate immunity. Trends Genet. 9: 178-183. Iketani, M. and Morishima, I. 1993. Induction of antibacterial protein synthesis by soluble peptidoglycan in isolated fat body from larvae fo the silkworm, Bombyx mori. Insect Biochem. Molec. Biol. 23: 913-917. Keppi, E., Zachary, D., Robertson, M., Hoffmann, D. and Hoffmann, J. A. 1986. Induced antibacterial proteins in the haemolymph of Phormia terranovae ( Diptera ) purification and possible origrin of one protein. Insect Biochem. 16: 395-402. Kaaya, G. P., Flyg, C. and Boman. H. G. 1987. Insect immunity, Induction of cecropin and attacin-like antibacterial factors in the haemolymph of Glossina mirsitans morsitans. Insect Biochem. 17: 3009-3015. Kockum, K., Faye, I., von Hofsten, P., Lee, J-Y., Xanthopoulos, K. G. and Boman, H. G. 1984. Insect immunity, Isolation and sequence of two cDNA clones corresponding to acidic and basic atticins from Hyalophora cecropia, EMBO J. 3: 2071-2075. Lee, J-Y., Boman, A., Chuanxin, S., Andersson, Mats., Jounvall, H., Mutt, V. and Boman, H. G. 1989. Antibacterial peptides from pig intestine: isolation of a mammalian cecropin. Proc. Natl. Acad. Sci USA 86: 9159-9162. Lemos, F. J. A. ，Ribeiro, A. F. and Treea, W. R. 1993. A bacteria-digesting midgut-lysozyme from Musca domestica ( Diptera ) larvae. purification, properties and secretory mechanism. Insect Biochem. Molec. Biol. 23: 533-541. Merril, C. R., Goldman, D., Sedman, S. A. and Ebert, M. L. 1981. Ultra-sensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 211: 1437-1438. Morishima, I., Suginaka, S., Ueno, T. and Hirano, H. 1990. Isolation and structure of cecropins, Inducible antibacterial peptides, from the silkworm, Bombyx mori. Comp. Biochem. Physiol. 95B, 3: 551-554. Morishima, I., YamadA, K. and Ueno, T. 1992. Bacterial peptidoglycan as elicitor of antibacterial protein synthesis in larvae of the silkworm, Bombyx mori. Insect Biochem. Molec. Biol. 22: 363-367. Nagamine, C., Knight, A. W., Maggenti, A. and Paxman, G. 1980. Effects of androgenic gland ablation on male primary and secondary sexual characteristics in the Malaysian Prawn，Macrobrachium rosenbergii(Decapoda, Palaemonidae ), with first evidence of induced feminization in a nonhermaphroditic decapod. Gen. Comp. Endocrinol. 41: 423-441. Palli, S. R. and Locke, M. 1987. The synthesis of hemolymph proteins by the larval midgut of an insect Calpodes ethlius ( Lesperiidae）. Insect Biochem. 17: 561-572. Phipps, D. J., Chadwick, J. S. and Aston, W. P. 1994. Ga/lysin-1, an antibacterial protein isolated from hemolymph of Galleria mellonella. Dev. Comp Immunol. 18: 13-23. Powning, R. F. and Davidson, W. J. 1976. Studies on insect bacteriolytic enzymes-II some physical and enzymatic properties of lysozyme form haemolymph of Galleria mellonella. Comp. Biochem. Physiol. 55B: 221-228. Qu, X.- m., Steiner, H., Engstrom, A., Bennich, G. and Boman, H. G. 1982. Insect immunity: Isolation and structure of cecropins B and D from pupae of the Chinese oak silk moth, Antheraea pernyi. Eur. J. Biochem. 127: 219-224. Robertson, M. and Postlethwait, J. H. 1986. The humoral antibacterial response of drosophila adults. Dev. Comp. Immunol. 10: 167-179. Samakovlis, C. ，Kimbrell, D. A., Kylsten, P.. Engstrom, A. and Hultmark, D. 1 990. The immune response in Drosophila: Pattem of cecropin expression and biological activity. EMBO J. 9: 2969-2976. Sipos, D. Andersson, M. and Ehrenberg, A. 1992. The stucture of the mammalian antibacterial peptide cecopin P1 in solution, determined by proton-NMR. Eur. J. Biochem. 209: 163-169. Soderhall, K. and Cerenius, L. 1992. Crustacean immunity. Annual Rev Fish Dis., pp. 3-23. Sumida, M. Ichimori, H., Johchi, S., Takaoka, A., Yuhki, T., Mori H. and Matsubara, F. 1992a. Antibactrerial activity inducible in the haemolymph of the silkworm, Bombyx mori, by injection of formalin-treated Escherichia coli K-12 during the fifth larval instar and pharate adult development Comp. Biochem. Physiol. 101B: 165-171. Sumida, M., Ichimori, H., Johchi, S., Takaoka, A., Yuhki, T., Mori, H., and Matsubara, F. 1992b, Induction of antibacterial activity in the haemolymph of the silkworm, Bornbyx mori, by injection of formalin-treated Escherichia coli K-12 in the anterior and posterior body part of the ligated larvae Comp, Biochem. Physiol. 101B: 173-178. Sun, S-C. and Faye, I. 1992. Cecropia immunoresponsive factor, an insect immunoresponsive factor with DNA-binding properties similar to nuclear-factor κ B. Eur. J. Biochem. 204: 885-892. Steiner, H., Andreu, D. and Merrifield, R. B. 1988. Binding and action of cecropin and cecropin analogues: antibacterial peptides from insects. Biochim. Biophy. Acta. 939: 260-266. Steiner, H., Hultmark D., Engstrom, A., Bennich, H. and Boman, H. G. 1981. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature. 292: 246-248. Stewart, J. E. and Zwicker, B. M. 1972. Natural and induced bactericidal activities in the hemolymph of the lobster, Homarus americanus: products of hemocyte-plasma interaction. Can. J ．Microbiol. 18: 1499-1509. Sun, S-C., Asling, B. and Faye I. 1991. Organization and expression of the immunoresponsive lysozyme gene in the ginat silk moth, hyalophora cecropia. J. Biol. Chem. 266, 6644-6649. Sun, S-C. and Faye, I. 1992a. CIF, an insect immunoresponsive facotor with DNA-binding properties similar to NF -x B. Eur. J. Biochem. 204. 885-892. Sun, S-C. and Faye, I. 1992b. Affinity purification and characterization of CIF, an insect immunoresponsive factor with NF-x B-like properties. Comp. Biochem. Physiol. 103B: 225-233. Wade, D., Boman. A., Wahlin, B., Drain, C. M., Andreu, D., Boman, H. G. and Merrifield, R. B. 1990 ，All-D amino acid-containing channel-forming antibiotic peptides. Proc. Natl. Acad. Sci. USA 87: 4761-4765. Zasloff, M. 1987. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sepuence of a precursor. Proc. Natl. Acad. Sci. 84: 5449-5453. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76080 | - |
| dc.description.abstract | 以常見之水產動物的病原菌為測試菌，利用抑菌圈分析法檢測 cecropin A、 cecropin P1 及溶菌酵素（lysozyme) 對不同菌種的抗菌能力，結果發現 cecropin 可以抑制淡水菌 Aeromonas hydrophila 及海水菌 A . salmonicida 之生長；但對淡水菌 Edwardsiella tarda沒有抗菌活性；除了從病鰻分離出的 Vibrio vulnificus 菌株外，cecropin 對其餘常見 10 種 Vibrio 菌都有抗菌活性；cecropin 對 2 株淡水酵母菌 Debaryomyces 有抗菌活性：對 3 株 E . coli D21 , D31 及JM107 亦有抗菌效果。溶菌酵素則僅對 2 株酵母菌 Debaryomyces 有抗菌活性。 以大腸桿菌 E . coli JM 107 為免疫刺激物，注射到淡水長臂蝦圍心腔內，經過 1 、 2 、 3 、 4 、 5 、 6 、 12 、 18 、 24 、 48 、 72 小時後，從圍心腔抽取血淋巴液，將抗凝處理過的血淋巴液離心取血漿，再利用超過濾膜略純化並縮減體積約三倍後利用抑菌圈分析法檢測其是否含抗菌物質。以大腸桿菌 JM107 及 D21 為測試菌，結果發現血漿中確實有抗菌物質被誘發出來。注射後 6 小時， 31％的測試蝦血漿開始出現抗菌活性，迄 24 小時，79％的測試蝦出現抗菌活性，至 72 小時所有蝦子的抗菌活性皆消失，顯示一旦被激發後多數測試蝦在血漿中會合成抗菌素，估算其濃度最高不會超過 0.21 μM，且為短效性。將含抗菌物質的蝦血漿跑SDS-PAGE，轉印至 PVDF 膜上，再以兔抗 cecropin A 血清作免疫染色，發現在 35kd 附近有一色帶，因此推測淡水長臂大蝦注射大腸桿菌後血漿中誘發的抗菌物質可能類似野蠶的 cecropin。 | zh_TW |
| dc.description.abstract | Cecropin A, cecropin P1 and lysozyme susceptibilities of the common aquatic pathogens were tested with an inhibition zone assay. Results showed that cecropins could inhibit growth of the 16 stains of bacteria, including Aeromonas hydrophila, A. salmonicida, Escherichia coli, Vibrio alginolyticus, V. anguillarum, V. carchariae, V. damsela, V. fluvialis, V. harveyi, V. ordalii ，V, parahemolytics ，V. tubiashii and V. vulnificus. Cecropins also inhibited growth of 2 pathogenic yeast stains, Debaryomyces hansenii. However, antibacterial activities of cecropins could not reach to Edwardsiella tarda, as well as, V. vulnificus strains isolated from a diseased eel. Microbicidal activity provided by the lysozyme only acted on D. hansenii. We used the inhibition zone assay to detecte the presence of antimicrobial substances produced by giant freshwater prawn (Macrobrachium rosenbergii). When prawn were injected into the pericardial cavity with E. coli cells, hemolymph were collected at 1,2,3,4,5,6,1,18,24,48, 72 hours post - injection. Assay of the plate showed an induced bacterial substance (s). The antibacterial activity appeared in 31 % of tested prawn plasma 6 hrs post- injection. Same activity was shown in 79 % of tested plasma 24 hrs post-injection. However, it was not detected in all tested plasma synthesize antibacterial substance (s) once stimulated. However ，this activity was short - term and concentration was estimated no more than 0.2luM in prawn plasma. Western blot analysis of plasma with an antibacterial acti-rabbit-anti-cecropin A serum. These results indicated that the antibacterial substance induced in giant freshwater prawn may be a cecropin-like. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:51Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 謝 誌……………………………………………………1 一、中文摘要…………………………………………………2 二、英文摘要…………………………………………………4 三、緒言………………………………………………………6 四、文獻回顧…………………………………………………8 五、材料與方法………………………………………………19 六、結果………………………………………………………29 七、討論………………………………………………………34 八、參考文獻…………………………………………………42 九、附圖………………………………………………………52 十、附表………………………………………………………56 十一、附錄……………………………………………………60 | |
| dc.language.iso | zh-TW | |
| dc.title | 淡水長臂大蝦血漿內抗菌素的分析 | zh_TW |
| dc.title | Stimulation of Giant Freshwater Prawn （Macrobrachium rosenbergii ) to Generate the Antimicrobial Substances - Analysis of the Cecropin - like Peptide | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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