請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76060完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 劉珍 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:40Z | - |
| dc.date.available | 2021-07-01T08:17:40Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Andersson, K.B., Sletten K., Berntzen, H.B., Dale, I., Brandtzaeg, P., Jellun, E., and Fagerhol, M.K. (1988) The leucocyte L1 protein: identity with the cystic fibrosis antigen and the calcium-binding MRP-8 and MRP-14 macrophage components. Scand. J. Immunol. 28, 241-245.
Aubert, D., Garcia, M., Benchaibi, M., Poncet, D., Chebloune, Y., Verdier, G., Nigon, V., Samarut, J., and Mura C.V. (1991) Inhibition of proliferation of primary avian fibroblasts through expression of histione H5 depends on the degree of phosphorylation of the protein. J. of Cell Biol. 113, 497-506. Bamberger, D.M., Herndon, B.L., and Suvarna, P.R. (1993) The effect of zinc on microbial growth and bacterial killing by cefazolin in a Staphylococcus aureus abscess milieu. J of Infectious Disease 168, 893-896. Barraclough, R., Savin, J., Dube, S.K., Rudland, P.S. (1987) Molecular cloning and sequence of the gene p9Ka. A cultured myoepithelial cell protein with strong homology to S-100, a calcium-binding protein. J. Mol. Biol. 198, 13-20. Bhardwaj, R.S., Zotz, C., Zwadlo-Klarwasser, G., Roth, J., Goebeler, M., Mahnke, K, Falk, M., Meinardus-hager, G., and Sorg, C. (1992) The calcium-binding proteins MRP8and MRP14 form a membrane-associated heterodimer in a subset of monocytes/macrophages present in acute but absent in chronic inflammatory lesions. Eur. J. of Immunol. 22, 1891-1897. Brandtzaeg, P., Dale, I., and Fagerhol, M.K (1987) Distribution of a formalin-resistant myelomonocytic antigen (L1) in human tissue. II. Normal and abrrent occurence in various epithelia. Am. J. Clin. Pathol. 87, 700-707. Brandtzaeg, P. (1988) The new monoclonal antibody (Mac-387) that reacts with macrophages on paraffin sections detects the well-known leukocyte L1 antigen. J. Histochem. Cytochem. 36, 1203-1204. Brantzaeg, P., Dale, I., and Gabrielsen, T.-O. (1992) The leukocyte protein L1 (calprotectin): usefullness as an immunohistochemical marker antigen and putative biological function. Histopathology 21, 191-196. Bruggen, J., Tarcsay, L., Cerletti, N., Odink, K., Rutishauser, M., Hollander, G., and Sorg, C. (1988) The molecular nature of the cystic fibrosis antigen. Nature 331, 570. Bullock, S., Haywark, c., Manson, J., brockm D.J.H., and Raeburn, J.A. (1982) Quantitative immunoassays for diagnosis and carrier detection in cystic fibrosis. Clin. Genet. 21, 336-341. Clark, B.R., Kelly, S.E., and Fleming S. (1990) Calgranulin expression and association with the keratinocyte cytoskeleton. J. of Pathology 160, 25-30. Dale, I., Fagerhol, M.K., and Naesgaard, I. (1983) Purification and partial characteriation of a highly immunogenic human leukocyte protein, the L1 antigen. Eur. J. Biochem. 134, 1-6. Delabie, J., de Wolf-Peeters, C., van den Oord, J.J., and Desmet, V.J (1990) Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study. Clin. Exp. Immunol. 81, 123-126. Dianoux, A.C., Stasia, M.J., Garin, J., Gagnon, J., and Vignais, P.V. (1992) The 23-kilodalton protein, a substrate of protein kinase C, in bovine neutrophil cytosol is a member of the S100 family. Biochemistry 31, 5898-5905. Doram, T.I., Vidrich, A., and Sun, T.T. (1980) Intrisic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22, 17-25. Dorin, J.R., Novak, M., Hill, r.E., Brock, D.J.H., Secher, D.S., and van Heyning, V. (1987) A clue to the basic defect in cystic fibrosis form cloning the CF antigen gene. Nature 326, 614-617. Ebralidze, A., Tulchinsky, E., Grigorian, M., Afanasyeve, A., Viacheslav, S., Rexazova, E., and Lukannidin, E. (1989) Isolation and characterization of a gene specifically expressed in different metastatic cells and whose deduced gene product has a high degree of homology to a Ca2+-binding protein family. Genes Devel. 3, 1086-1093. Edgeworth, J., Gorman, M., Bennett, R., Freemont, P., and Hogg, N. (1991) Identification of p8, 14 as a highly abundant heterodimer calcium binding protein complex ofmyeloid cells. J. Biol. Chem. 266, 7706-7713. Eversole, L.R., Miyasaki K.T. and Christensen, R.E. (1992) The distribution of the anti-microbial protein, calprotectin. in normal oral keratinocyte. Arch. oral Biol. 37, 963-968. Feari, S., Calabretta, B., de Riel, J.K., Battini, R., Ghezzo, F., Lauret, E., Griffin, C., Emanuel, B.S., Gurrieri, F., and Baserga, R. (1987) Structural and functional analysis of a growth-regulated gene, the human calcyclin. J. Biol. Chem. 262, 8325-8332. Gabrielsin T.-O., Dale, I., Brandzaeg, P., Hoel, P.S., Fagerhol, M.K., Larsen, T.E., and Thune, P.O. (1986) Epidermal and dermal distribution of a myelomonocytic antigeh (L1) shared by epithellial cells in various inflammatory skin diseases. J. Am. Acad. Dermatol. 15, 173-179. Gerke, V., and Weber, K. (1984) Identity of p36 phosphorylated upon Rous sarcoma virus transformation with a protein purified from brush borders; calcium-dependent binding to nonerythroid spectrin and F-actin. EMBO J. 3, 227-233. Glenney, J.R., Kindy, M.S., and Zokas, L. (1989) Isolation of a new member of the S-100 protein family: amino acid sequence, tissue, and subcellular distribution. J. Cell Biol. 108, 569-578. Goebeler, M., Roth, J., Henseleit, U., Sunderkotter, C., and Sorg, C. 91993) Expression and complex assembly of calcium-binding proteins MRP8 and MRP 14 during differentiation of murine myelomonocytic cells. J. of Leukocyte Biol. 53, 11-18. Gomez-Cuadroda, A., rousseau, S., Renaud, J., and Ruiz-Carrillo, A. (1992) Repression of the H5 histion gene by a factor from erythrocyte that binds to the region of transcription initiation. EMBO J. 11, 1857-1866. Goto, K., Endo, H., and Fujiyoshi, T. (1988) Cloning of the sequences expressed abundantly in established cell lines: identification of a cDNA clone highly homologus to S-100, a calcium binding protein. J. Biochem. 103, 48-53. Graziano, V., Gerchman, S.E., Wonocott, A.J., Sweet, R.M., Wells, J.R., White, S.W., and Ramakrishnan, V. (1990) Crystallization of the globular domain of histone H5. JofMol. Biol. 212, 253-257. Heizmann, C.W. (1991) Calcium-binding proteins of the EF-hand-type and of the annexin family: a survey. InNovel Calcium-binding Proteins. Fundamentals and Clinical Implications, (ed. C.W. Heizmann). pp39-49. Berlin: Springer-Verlag. Heizmann, C.W., and Hunziker, W. (1991) Intracellular calcium-binding proteins: more sites than insights. TIBS 16, 98-103. Hessian, P.A., Edgeworth, J., and Hogg, N. (1993) MRP-8 and MRP-14, two abundant Ca2+- binding proteins of neutrophils and monocytes. J. of Leukocyte Biology 53, 197-204. Hilt, D.C., and Kligman, D. (1991) The S-100 protein family: a biochemical and functional overview. In Novel Calcium-binding Proteins. Fundamentals and Clinical Implications (ed. C.W. Heizmann), pp. 65-103. Berlin: Springer-Verlag. Hogg, N., Allen, C., and Edgeworth, J. (1989) Monoclonal antibody 5.5 reacts with p8, 14, a myeloid molecule associated with some vascular endothelium. Eur. J. Immunol. 19, 1053-1061. Imamichi, T., Uchida, I., Wahl, S.M., and McCartney-Francis, N. (1993) Expression and cloning of migration inhibitory factor-related pretein (MRP) 8 and MRP14 in arthritis-susceptible rats. Biochem. Biophy. Res. Commun. 194, 819-825. Jackson-Grusby, L.L., Swiergiel, J., and Linzer, D. (1987) A growth-related mRNA in cultured mouse cells encodes a placental calcium-binding protein. Nuc. Acids Res. 15, 6677-6690. Kligman, d., and Hilt, D.C. (1988) The S-100 protein family. TIBS 13, 437-443. Koike, T., Morikawa, M., Hashimoto, S., Harada, N., Yoshida, T. A possible regulatory role of calcium binding protein MRP-14 in the differentiation of myeloid cell lines. J. Cell Biol. 111, 356a. Kretsinger, R. (1980) Structure and evolution of calcium modulated proteins. CRC Crit. Rev. Blochem. 8, 119-174. Kunz M., Roth, J., and Kolde, G. (1992) Epidermal expression of the calcium binding surface antigen 27E10 in inflammatory diseases. Arch. Dermatol. Res. 284, 386-390. Kuwayama, A., Kuruto, R., Horie, N., Takeishi, K., and Nozawa, R. (1993) Appearence of nuclear factors that interact with genes for myeloid calcium binding proteins (MRP-8 and MRP-14) in differentiated HL-60 cells. Blood 81, 3116-3121. Lagasse, E., and Clerc, R.G. (1988) Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation. Mol. Cell Biol. 8, 2402-2420. Lagasse, E., and Weissman, I.L. (1992) Mouse MRP8 and MRP14, two intracellular calcium-binding proteins associated with development of myeloid lineage. Blood 79, 1907-1915. Lemarchand, P., Vaglio, M., Mauel, J., and Markert, M. (1992) Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation. J. Biol. Chem. 267, 19379-19382. Longbottom, D., and van Heyningen V. (1991) The calgranulins, members of the S-100 protein family:structural features, expression, and possible function. In Novel Calcium-binding Proteins. Fundamental and Clinical Implications (ed. C. W. Heizmann), pp226-236. Berlin: Springer-Verlag. Longbottom, D., Sallenave, JM., and van Heyning, V. (1992) Subunit structure of calgranulins A and B obtained from sputum, plasma, granulocytes, and cultured epithelial cells. Biochimica et Biophysica Acta 1120, 215-222. Masiakowaki, P., and Shooter, E.M. (1988) Nerve growth factor induces the genes for two proteins related to a family of calcium-binding proteins in PC12 cells. Proc. Natl. Acad. Sci. 85, 1277-1281. Moore, B. (1965) A soluble protein characterisitic of the nervous system. Biochem. Biophys. Res. Comm. 19, 739-744. Moore, B. (1984) The S-100 protin, In: Marangos, P.J., Cambell, I.C., and Cohen, R.M. (eds) Neuronal and glialproteins. Academic Press Pub, San Diego, 739-744. Moss, S.E., and Crumpton, M.J. (1990) The Lipocortins and the EF-hand proteins: Ca-binding stie and evolution. TIBS 15, 11-12. Murao, S., Epstein, A.L., Clevenger, C.V., and Huberman, E. (1985) Expression of maturation-specific nuclear antigens in differentiating human myeloid leukemia cells. Cancer Research 45, 791-795. Murao, S., Collart, F.R., and Huberman, E. (1989) A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases. J. Biol. Chem. 264, 8356-8360. Murao, S., Collart, F.R., and Huberman, E. (1990) A protein complex expressed during ternminal differentiation of monomyelocytic cells in an inhibiton of cell growth. Cell Growth Differ. 1, 447-454. Murthy, A.R. K., Lehrer, R.I., Harwug, S.S.L., and Miyasaki, K.T. (1993) In vitro candidastatic properties of the human neutrophil calprotectin complex. J. of Immunology 151, 6291-6301. Nozawa, R., Kato, H., Ito, T., and Yokoto, T. (1988) Identification and characterization of a differentiation antigen in human neutrophils. Blood 71, 1288-1294. Odink, K., cerletti, N., Bruggen, J., Clerc, R.G., Tarcsay, L., Zwadlo, G., Gerhards, G., Schlegel, R., and Sorg, C. (1987) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature 330, 80-82. Penner, C.G., and Davie, J.R. (1992) Multisubunit erythroid complexes binding to the enhancer element of the chicken histone H5 gene. Biochem. J. 283, 905-911. Pikaart, M., Irving, J., and Villeponteau, B. (1991) Decline in histione H5 phosphorylation during erythroid senescence in chick embryos. Mechanisms of Ageing and Development 59, 189-195. Ramakrishnan, V., Finch, J.T., Graziano, v., Lee, P.L., and Sweet R.M. (1993) Crystal structure of globular domain of histone H5 and its implications for nucleosome binding. Nature 362, 219-223. Roth, J., Teigelkamp, S., Wilke, M., Grun, L., Tummler, B., and Sorg, C. (1992) Complex patterm of the myelo-monocytic differentiation antigens MRP8 and MRP 14 during chronic airway inflammation. Imunobiol. 186, 304-314. Roth, J., Burwinkel, F., van den Bos, C., Goebeler, M., Vollmer, E., and Sorg, C. (1993) MRP8 and MRP14, S-100-like proteins associated with myeloid differentiation, are translocated to plasma membrane and intermediate filaments in a calcium-dependent manner. Blood 82, 1875-1883. Rousseau, S., Asselin, M., Renaud, J., and Ruiz-Carrillo, A. (1993) Transcription of the histone H5 gene is regulated by three differentiation-specific enhencers. Molecular & Cellular Biol. 13, 4904-4917. Schafer, T., Sachse, G.E., and Gassen, H.G. (1991) The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells. Biological Chemistry Hoppe-Seyler 372, 1-4. Schleicher, C.H., Dell'Angelica, E.C., and Santome, J.A. (1993) Isolation and N-terminal sequence of two low molecular weight calcium-binding proteins from pig granulocytes. Int. J. Biochem. 25, 1251-1256. Segers, A., Wyns, L., and Lasters, I. (1991) A model for histone H5-DNA interaction: simultaneous minor and major groove binding. Biochem. Biophys. Res. Commun. 174, 898-902. Sellmayer, A., Krane, S.M., Ouellette, A.J., and Bonventre, J.V. (1992) 1α, 25-(OH)2 vitamin D3 enhances expression of the genes encoding Ca2+-binding proteins MRP-8 and MRP-14. Am. J. Physiol. 262, C235-C242. Sohnle, P.G., Colins-Lech, C., and Wiessner, J.H. (1991a) Antimicrobial activity of an abundant calcium-binding protein in the cytoplasm of human neutrophils. J. of Infectious Diseases 163, 187-192. Sohnle, P.G., Collins-Lech, C., and Wiessner, J.H. (1991b) The zinc-revesible antimicrebial activity of neutrophil lysates and abscess fluid supernatants. J of Infectious Diseases 164, 137-142. Stasia, M., Dianoux, a., and Vivgnais, P.V. (1989) A 23-kDa protein as a substrate for protein kinase C in bovine neutrophils. purification and partial characterzation. Biochemistry 28, 9659-9667. Stein, G.S., Stein J.L., and Marzluff, W.F. (1984) Histone Genes. John Wiley & Sons, Inc. New York. Steinbakk, M., Naess-Andresen, C.-F. Lingaas, E., Dale, I., Brandtzaeg, P., and Fagerhol, M.K. (1990) Antimicrobial actions of calcium binding leukocyte L1 protein, calprotectin. Lancet 336, 763-765. Sun, J.M., Penner, c.G., and Davie, J.R. (1992a) Analysis of erythroid nuclear proteins binding to the promoter and enhancer elements of the chicken histone H5 gene. Nucleic Acids Research 20, 6385-6392. Sun, J.M., Hendzel, M.J., and davie, J.R. (1992b) Nuclear matrix proteins bind very tightlly to specific regions of the chicken histone H5 gene. Biochem. and Cell Biol. 70, 822-829. Sun, J.M., Penner, C.G., and Davie, J.R. (1993) Repression of histone H5 gene expression in chicken mature erythrocytes is correlated with reduced DNA-binding activities of transcription factors Sp1 and GATA-1. FEBS Letters 331, 141-144. Tang, T.K., Hong, T.-M., Lin, C.-Y., Lai, M.-L., Liu, C.H.L., Lo, H.-J., Wang, M.-E., Chen L.B., Chen, W.-T., Ip W., Lin, D.C., Lin, J. J.-C., Lin, S., Sun, T.-T., Wang, E., Wang, J.L., Wu, R., C.-W., Wu, and Chien, S. (1993) Nuclear proteins of the bovine esophageal epithelium. I. Monoclonal antibody W2 specifically reacts with condensed nuclei of differentiated superficial cells. J of Cell Sci. 104, 237-247. Teigelkamp, S., Bhardwaj, R.S., Roth, J., Meinardus-Hager, G., Karas, M., and Sorg, C. (1991) Calcium-dependent complex assembly of the myeloid differentiation proteins MRP-8 and MRP-14. J. Biol. Chem. 266, 13462-13467. Tobe, T., MUrakami, K., Tomita, M., and Nozawa, R. (1989) Amino acid sequences of 60B8 antigens induced in HL-60 cells by 1, 25-dihydroxyvitamin D3. the antigens are indential with macrophage-related protein-14 and -8. Chem. Pharm. Bull. 37, 1576-1580. Tsai, Y.H., and Hnilien, L.S. (1975) Tissue-specific histones in the erythrocytes of chicken and turtle. Exp. Cell Res. 91, 107-112. van Roozendaal, K.E.P., darling, D., and Farzaneh, F. (1990) DMSO and retinoic acid induce HL-60 differentiation by different but converging pathways. Exp. Cell Res. 190, 137-140. Warner-Bartnicki, A.L., Murao, S., Collart, F.R. and Huberman, E. (1993) Regulated expression of the MRP8 and MRP14 genes in human promyelocytic leukemic HL-60 cells treated with the differentiation-inducing agents mycophenolic acid and 1α, 25-dihydroxyvitamin D3. Exp. Cell Res. 204, 241-246. Wilkinson, M.M., Busuttil, A., Hayward, c., Brock, D.J.H., Dorin, J.R., and van Heyning, V. (1988) Expression pattern of two related cystic fibrosis - associated calcium-binding proteins in normal and abnormal tissues. J. Cell Sci. 91, 221-230. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76060 | - |
| dc.description.abstract | 為研究分化相關的細胞核蛋白質，本實驗室曾以牛食道上皮細胞核萃取物，製備了一系列之單株抗體，並篩選出一株可以專一地認得牛食道上皮末期分化細胞緻密核的單株抗體W2。利用免疫沈澱法分離W2所認得的抗原，得到了－33 kDa的蛋白質複合體。此複合體又可以還原分離為－11kDa及－22kDa的蛋白質，分別稱為牛食道上皮蛋白（Bovine Esophageal Epithelium），BEE11及BEE22。在本論文中，我選殖出BEE11完整之genomic DNA序列，並利用基因資料庫尋找相似的基因進行分析與比對，發現BEE11與一人類白血球分化調節基因，MRP（migration inhibitory factor-related protein）8，在序列比較上有極大的相似性。此外，我利用E. coli expression vector製備了BEE11之重組蛋白，及產生具有高度專一性之多源抗體。以Northern及Western Blot分析BEE11在牛各組織中的表現，顯示BEE11主要分佈在牛食道與牛舌等上皮細胞組織中。 | zh_TW |
| dc.description.abstract | Some nuclear proteins that exhibit unique expression patterns may play important roles in the differentiation program. In order to study the cell differentitaion mechanisms, a series of monoclonal antibodies that against nuclear extracts isolated from bovine esophageal epithelium were raised by our laboratory. One of these monoclonal antibodies, W2, stained specifically at the condensed nuclei of the superficial epithelium, but not the cells at the basal stratum of bovine esophagus. This specific staining pattern suggested that W2 antigen may be involved in epithelial cell differentiation. Immunoisolation of W2 target antigen from the nuclear extracts of superficial cells yielded a 33kDa protein complex named as bovine esophageal epithelium (BEE)33. Upon DTT reduction, BEE33 dissociated into two polypeptides, BEE11 and BEE22. In this study, I have isolated the BEE11 gene from a bovine genomic library and have characterized its genomic structure. My results showed that BEE11 gene shared high degree of similarity with human MRP (migration inhibitory factor-related protein) 8 gene. A polyclonal antibody that specifically against BEE11 protein was also raised. Northern and Western blot analyses were used to investigate the distribution of BEE11 in various bovine tissues at both mRNA and protein levels. My results show that BEE11 appears to be mainly expressed at bovine esophagus and tongue tissues. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:40Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 第一章 緒 論……………………………………1 第二章 材料與方法……………………………………12 2-1 實驗材料……………………………………12 2-2 實驗藥品及緩衝液……………………………………13 2-3 Genomic DNA株之分離——菌落雜交法（plague hybridization）……………………………………15 2-3-1 平皿培養基因庫及濾膜複印（Library Plating and Filter Replica）……………………………………15 2-3-2 以DNA探針進行雜交篩選（Hybridization with DNA Probe）……………………………………16 2-4 重組λ噬菌體DNA的製備（Recombinant Phage DNA Preparation）……………………………………17 2-5 DNA轉印分析（Southern Blot Analysis）……………………………………18 2-6 由洋菜凝膠中提取DNA片段（DNA Extraction from Agarose Gels）……………………………………19 2-6-1 由低熔點洋菜凝膠中提取DNA……………………………………19 2-6-2 由一般洋菜凝膠中提取DNA……………………………………19 2-7 接合作用（ligation）……………………………………20 2-8 轉化作用（Transformation）……………………………………20 2-9 質體DNA的小量製備（Mini-preparation of Plasmid DNA）……………………………………21 2-10 DNA定序反應（DNA Sequencing）……………………………………22 2-10-1 準備定序反應物……………………………………22 2-10-2 定序凝膠的製備……………………………………23 2-10-3 定序電泳……………………………………23 2-10-4 膠片固定乾燥及自動放射顯影……………………………………24 2-11 DNA、蛋白質序列比對分析……………………………………24 2-12 Polymerase Chain Reaction及以pCRII載體選殖基因……………………………………24 2-13 重組蛋白的生產……………………………………25 2-14 多源抗體的製備（Production of Polyclonal Antibodies）……………………………………26 2-15 由牛組織抽取total RNA……………………………………27 2-16 RNA轉印分析（Northern Blot Analysis）……………………………………27 2-16-1 甲醛凝膠的製備……………………………………28 2-16-2 RNA電泳及轉印……………………………………28 2-16-3 DNA-RNA Hybridization……………………………………29 2-17 製備牛組織之蛋白質萃取物……………………………………29 2-18 免疫轉印分析（Immunoblot Analysis）……………………………………30 第三章 結 果……………………………………31 3-1 BEE11基因的分離與再選殖……………………………………31 3-1-1 自bovine genomic DNA library中篩選出BEE11的基因株……………………………………31 3-1-2 以restriction enzyme圖譜分析BEE11 positive clones……………………………………31 3-1-3 利用南方轉印分析BEE11 genomic DNA片段以進行再選殖……………………………………32 3-2 BEE11基因的定序與構造分析……………………………………33 3-2-1 BEE11基因的定序……………………………………33 3-2-2 BEE11基因的構造……………………………………34 3-3 BEE11與相關基因的對照分析……………………………………35 3-3-1 BEE11蛋白質與其他S100家族蛋白質的比對分析……………………………………35 3-3-2 BEE11蛋白質與不同種動物之MRP8蛋白質的比對分析……………………………………36 3-3-3 BEE11與人類MRP8完整基因的對照和比較……………………………………37 3-4 BEE11重組蛋白與多源抗體的製備……………………………………39 3-4-1 利用PCR選殖BEE11完整之轉譯序列……………………………………39 3-4-2 BEE11重組蛋白的生產……………………………………39 3-4-3 BEE11多源抗體的產生與專一性的測試……………………………………40 3-5 BEE11組織特定性表現之研究……………………………………41 3-5-1 BEE11 mRNA在不同牛組織的表現分析……………………………………41 3-5-2 BEE11蛋白質在不同牛組織的表現分析……………………………………42 第四章 討 論……………………………………43 | |
| dc.language.iso | zh-TW | |
| dc.title | 食道上皮細胞分化核蛋白之分子選殖及特性之研究 | zh_TW |
| dc.title | Molecular Cloning and Characterization of A Bovine Esophageal Epithelial Nuclear Protein Involved in Epithelial Cell Differentiation | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 88 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。