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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lisa Lee | en |
dc.contributor.author | 李麗莎 | zh_TW |
dc.date.accessioned | 2021-07-01T08:17:14Z | - |
dc.date.available | 2021-07-01T08:17:14Z | - |
dc.date.issued | 1977 | |
dc.identifier.citation | 1. Bouillenne, R. and F. W. Went. 1933. Recherches exp?rimentales sur la n?oformation des racines dans les plantules et les boutures des plantes sup?rieures. Ann. Jard. Botan. Buitenzorg. 43:25-202. 2. Brian, P. W., H. G. Hemming, and D. Lowe. 1960, Inhibition of rooting of cuttings by gibberellic acid. Annals of Botany. 24: 407-418. 3. Chin, T. Y., M, M. Meyer, Jr., and L Beevers. 1969. Atbscisic acid stimulated rooting of stem cuttings. Planta (Ben.) 88:192-195. 4. Chang, W. C. and S. T. Chan. 1975. Cytochemical studies on the changes of nuclear histones and RNA synthesis activity of adventitious root initials in mung bean hypocotyl cuttings. Bot. Bull. Academia. Sinica. 17:192-202. 5. Chandra, G. Re., L. E. Gregory, and J. F. Wonley. 1971. Studies on the initiation of adventitious root on mung bean hypocotyl. Plant and Cell Physiol, 12:317-324. 6. Dore, J. 1965. Physiology of regeneration in cormophytes, Encyclopedia of Plant Physiol. 15:1-91. 7. Eriksen, E. N. 1973. Root formation in pea cuttings I. Effects of decapitation and disbudding at different developmental stages, Physiol. Plant. 28:503-506. 8. Fadl, J. S. and H. T. Hartmann. 1967. Isolation, purification, and characturization of an endogenous root-promoting factor obtained from basal sections of per hsrdwood cuttings. Plant Physiol. 42:54l-549. 9. Fellenberg, G. 1966. Die Hemmung auxininduzierter wulzelbildung an etidierten orbsenopicotylen mithlisten und antimetaboliten der RNS-und proteins-ynthese. Planta (Ben.) 71:27-42. 10. Guildfoyle, J. J., C. Y. Lin, Y. H. Chon, R. T. Nagao, and J. L. Key. 1975. Enhancement of soybean RNA polymerase I by auxin Proc. Nat. Acad. Sci. USA 72:9-72. 11. Girouard, R. H. 1968. Physiological and biochemical studies of adventitious root formation. Extractible rooting cofactors from Hedera helix. Can. J. Botany. L7:687-699. 12. Hansen, J. and H. N. Eriksen. 1974. Root formation of pea cuttings in relation to the irradiance of the stock plants. Physiol. Plant. 32:170-173. 13. Hansen, J. 1975. Light dependent promotion and inhibition of adventitious root formation by gibberellic acid. Planua (Berl.) 123:203-205. 14. Hess, C. E. 1957. A physiological analysis of rooting in cuttings of juvenile and mature Hedera helix L. Ph. D. Thesis. Cornell Univereity, Ithaca, New York. 15. Hills, D. C, and J. Horowitz. 1966. Ribosome synthesis in Escherichia coli treated with 5-Fluorouracil. Biochemistry. 5:1625-1632. 16. Jackson, M B. and P. M. Harney. 1970. Rooting cofactors, indoleacetic acid, and adventitious root initiation in mung bean cuttings (Phaseolus aureus). Can. J. Bot. 48:943-946. 17. Jalouzot, R, 1970. Etude du metabolisme des acid nucleiques et des peoteines au cours de rocines adventitious. Planta (Berl.) 97:16-27. 18. Krishnamoorthy, H, N, 1970, Promotion of rooting in mung bean hypocotyl cuttings with ethrel, an ethylene releasing compound, Plant and Cell Phybiol. 11:979-982. 19. Krul, W, R, 1968, Increased root initiation in pintobean hypocotyls with 24-dinitrophenol. Plant Physiol. 43:439-441. 20. Key, J. L., C, Y, Lin, E. Ii. Gifford, Jr., and R. Dengler, 1966, Relation of 2, 4-D-induced growth aberrations to changes in nucleic acid metabolism in soybean seedlings. Bot. 127 (2-3):87-94. 21. Lewin, B. 1974, control of transcription. In: Gene expression-2, Eucaryotic chromosome, John Wiley and Son., London: pp 320-575. 22. Libbert, E. 1956. Untersuchungen uber die physiologie der adventivwurzelbildung. I. Die wirkungsweise einiger komponenten dea Rhizokalinkomplexes. Flora. 144:121-150. 23. Lin, C. Y. and J. L. Key. 1966. Dissociation and reassembly of polyribosomes in relation to protein synthesis in the soybean root. J. Mol, Bid, 26:237-247. 24. Lin, C. Y. and J. L, Key. 1968. Cell longation in the soybean root: the influence of inhibitors of RNA and protein synthesis, Plant and Cell Physiol, 9:553-560. 25. Priestley, J. E. and C. F. Swingle. 1929. Vegetative propagation from the standpoint of plant anatomy. Tech. Bull. U. S. Dept. Agric, No 151. 26. Schultz, L, D. and B. D. Hall. 1976. Transcription in yeast : α-amanitin sensitivity and oter properties which distinguish between RNA polymerase I and III, Proc. Nat, Acad, Sci, USA 73:1089-1033 27. Sobota, A. E., C. J. Leaver, and J. L. Key. 1968, A detailed evaluation of the possible contribution of bacteria to radioactive precursor incorporation into nucleic acids of plant tissues. Plant Physiol, 43:907-913. 28. Veierskov, B., J. Hansen, and A. S. Andersen. 1976. Influence of Cotyledon excision and sucrose or root formation in ca cuttings. Physoil, Plant, 36:105-109. 29. Wu, C. H. and. C. Y. Lia 1968. The effect of chioramphenicol and actidione on chlorophyll synthesis. Taiwania, 14:1-10. 30.朱鈞、侯清利1972,植物荷爾蒙及生長調節劑之生物檢定法I. Auxin之生物檢定法。科學農業24(l-2):62-72。 31.蔡淑華1975。植物組織切片技術綱要。臺北:茂昌圖書公司。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76012 | - |
dc.description.abstract | 生長至第七天的綠豆幼苗切株,浸置蒸餾水中,四天後,在下胚軸基部會出現四行十字排列的不定根。若將切株浸置在NAA溶液,於不同時間再移入蒸餾水中,不定根仍與對照組同時長出,但NAA會促進不定根的數目,其中以處理24小時之切株,不定根數目增加最多。若將切株以四種生長激素:NAA、IBA、2,4-D和以IAA的最適濃度處理24小時後移入蒸餾水中,發現NAA的促進作用最大,可增加三倍以上的不定根數目,蛋白質或核糖核酸合成抑制劑,對不定根的影響為:cyoloheximide會抑制切株的生長,並使植物爛死;α-amanitin不會抑制不定根的生長;而5-FU會完全抑制不定根的發生,並使植物漸漸枯萎。 切株下胚軸基部相對於子葉和第一對小葉維管束跡的?皮部薄壁細胞,是不定根的起源。這些薄壁細胞於切株後36小時開始分裂,48小時形成根原,96小時突破表皮形成不定根。此不定根發生過程,NAA處理者與對照組投有顯著差異,僅36小時者有較顯著的細胞分裂現象。5-FU會抑制細胞分裂。若在細胞開始分裂之後,才將切株移置5-FU中,仍會抑制不定根的形成。 若將切株浸置蒸餾水或NAA溶液24小時後,切取下胚軸,以合有1μCi/ml:H-uridine的培養液培養2小時,發現NAA可促進2倍的核糖核酸合成量。若先用抑制劑preincubation2小時,得知,α-amanitin不會抑制核糖核酸的合成量,5-FU會抑制約60%的核糖核酸合成量。 推測,不定根發生以前,必需有RNA的台成。並且在不定根生長過程中,必需不斷地有rRNA的合成,促使細胞不斷地分裂,以產生根原,形成不定根。 | zh_TW |
dc.description.abstract | The formation of adventitious roots was studied using 7-day-old mung bean hypocotyl cuttings. The cuttings were presoaked in auxins for different periods followed by transfer into distilled water for 3 days. The cuttings presodked for 24 hrs in NAA at a concentration of 1×10^(-4)M produced more adventitious roots than at any other concentration of used and more than in any other auxin tested. The basal part of the cuttings formed the roots in four rows; these originated from the phloem parenchyma cells. Cell division of those parenchyma cells was evident after 36 hrs of incubation in distilled water. The formation of adventitious roots was irhibited by cycloheximide (5μg/ml). and 5-fluorouracil (1×10^(-3)M) but not by α-amanitin (1μg/ml). Presence of 5-FU in the beginning of the incubation period in distilled water following 24 hrs presoaking, inhibited the cell division and consequently inhibited the formation of root initials. 5-FU also inhibited the formation of adventitious roots even after coil divisions had occurred. Using 8^Huridine as a tracer, RNA synthesis was measured before any cell division had occurred in the root initials. NAA stimulated the RNA synthesis nearly 2 fold and this was inhibited by 5-FU. It is concluded that rRNA synthesis is essential before cell division can occur in the formation of root initials. The ultimate formation of adventitious roots from root initials also requires continuous coil division and also continuous synthesis of rRNA. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:14Z (GMT). No. of bitstreams: 0 Previous issue date: 1977 | en |
dc.description.tableofcontents | 中文摘要………………………………………1 英文摘要………………………………………3 緒言………………………………………5 材料與方法……………………………………10 結果………………………………………14 討論………………………………………34 引用文獻………………………………………39 | |
dc.language.iso | zh-TW | |
dc.title | 綠豆幼苗下胚軸不定根發生機制的研究 | zh_TW |
dc.title | Studies on the formation of adventitious root initial in mung bean seedling hypocotyl | en |
dc.date.schoolyear | 65-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 43 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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