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DC 欄位 | 值 | 語言 |
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dc.contributor.author | 林仲彥 | zh_TW |
dc.date.accessioned | 2021-07-01T08:17:12Z | - |
dc.date.available | 2021-07-01T08:17:12Z | - |
dc.date.issued | 1993 | |
dc.identifier.citation | Abramowitz, A. A., Hisaw, F. L. and Pappandrea, D. N. 1944. The occurrence of a diabetogenic factor in the eyestalks of crustaceans. Biol. Bull. 86: 1-5.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76008 | - |
dc.description.abstract | 甲殼類眼柄( Eyestalk . ES )內之竇腺組織( Sinus gland . SG ) 中,含有各種由髓部末梢 X-Organ ( Medulla terminalis X-organ , MTXO )所分泌的神經內分泌( Neurohormone ) ,這些神經內分泌調控著甲殼類的各種生理現象,並維持體內的恆定狀態,所以,經常應用於種蝦催熟之眼柄摘除法( Eyestalk ablation )往往會造成生理上極大的衝擊。 甲殼類高醣激素( Crustacean Hyperglycemic Hormone ,CHH ) 為眼柄竇腺組織中相當重要的神經內分泌,其主要之生理功能為醣類代謝調控,同時亦與脫殼週期、生殖繁衍有極密不可分的關係。 本實驗以臺灣之主要養殖蝦種-草蝦( Tiger shrimp , Penaeus , monodon )為實驗材料,進行血醣基礎代謝變化及 CHH 的生理調控之相關研究。利用組織學的觀察,以證實剝除部份結構後,所取出之眼柄確保有竇腺組織。進一步利用醋酸加熱萃取、離心 、過濾及一次高效液相管柱層析( HPLC )等純化步驟,配合以生物活性測定,發現有二個蛋白質具有 CHH 的活性,分別命名為 CHH I , CHH II 。 藉由 HPLC 進一步純化鑑定、氨基酸組成分析發現: CHH I 及 CHH II二者在吸收光譜、氨基酸組成上,都具有相當類似的性質,未來若更進於研究其氨基酸序列、蛋白質構造及訊息傳遞途徑,將可對其生理調控之分子機制有更深一層的瞭解。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:12Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
dc.description.tableofcontents | 摘要 . . . . . . . . . . . I 壹、前言 . . . . . . . . . . . 1 貳、材料及方法 . . . . . . . . . . . 7 一、眼柄之來源及保存 二、眼柄之切片及 H-E 染色 三、眼柄之萃取過程 四、血醣測定法 五、高效液相層析(HPLC ) 六、生物活性測定法 七、胺基酸組成分析法 八、尿素-SDS-聚丙醯銨膠體電泳 參、結果 . . . . . . . . . . . 18 一、眼柄之處理及保存 二、組織學上的研究 三、血醣的日夜週期變化 四、粗萃取液之純化與鑑定 五、高效液相層析分析 六、再次生物活性之測定 七、進一步的高效液相層析分析 八、胺基酸分析 九、電泳之分析 肆、討論 . . . . . . . . . . .24 一、高醣激素的純化鑑定與組織學研究 二、血醣及高醣激素的日周變化 三、高醣激素之胺基酸組成與其他甲殼類的比較 伍、參考文獻 . . . . . . . . . . . 31 陸、圖表 . . . . . . . . . . . 45 | |
dc.language.iso | zh-TW | |
dc.title | 草蝦高醣激素之純化與特性研究 | zh_TW |
dc.title | IDENTIFICATION AND CHARACTERIZATION OF CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) FROM TIGER SHRIMP, PENAEUS MONODON | en |
dc.date.schoolyear | 81-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 79 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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