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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 鄭恆仲 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:09Z | - |
| dc.date.available | 2021-07-01T08:17:09Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76003 | - |
| dc.description.abstract | 魚類卵細胞在環境誘導下，下視丘分泌的性釋素促進素（Gonadotropin Releasing Hormone , GnGH ）和性抑素（Gonadotropin Inhibiting Hormone ) ，調控腦下垂體促性腺激素（Gonadotropin , GtH ）之分泌。它刺激卵細胞濾泡層 ( Follicular layer ) ，經由類固醇生成作用產生雌二醇（Estradiol－17β, E2 ) ，E2經血液送到肝臟，誘導肝臟細胞合成卵黃前質（Vitellogenin ) ，再經由血液輸送，在卵細胞上以胞飲作用（Pinocytosis ）使卵黃前質累積於卵細胞中。 本研究以絨毛促性腺激素（Human Chorionic Gonadotropin , HCG ）、性釋素促進素類似物（ DesGly〔D－Ala6]－ethylamine analog ）等激素，探討促進卵細胞發育成熟之效果。 以HCG刺激處理，其效果以500 LU/Kg 最佳，促進肝臟合成卵黃前質之效果最快速，血液中卵黃前質量高，處理10天後在血液中即可檢測到Vg 之存在，隨劑量增加，效果反而減低。以 HCG 2000 LU/Kg處理者，容易造成死亡。至於 LHRH－a 處理，其促進卵巢成熟作用較HCG處理組慢，血液中卵黃前質量明顯比HCG處理組多，血液中Vg之出現於處理後90天，隨劑量增加，效果更顯著。LHRH－a 之處理，埋植處理之效果，比注射處理方式有效。 LHRH－a 及 LHRH－ a 加Domperidone 處理組，對鰻魚成熟沒有作用。 Estradiol l7β－ p 注射處理，能誘發肝臟合成Vg ，處理後第七天即可在血液中檢測到Vg 存在，隨著血液中Vg 量增加，粗脂肪量亦相對地升高，其中極性脂質之含量程現先增加後減少之現象，其原因尚未能鑑定。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:09Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 摘要• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • i 壹．前言• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 1 貳．材料與方法 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 5 一．材料來源及處理• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 5 二．試驗處理• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 6 三．卵黃前質之辨認．• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 7 四．高效陰離子交換層析法之卵黃前質之純化（Vitellogenin Purification by High performance Anion－ Exchange Chromatography ) • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 8 五．抗體之製備 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 8 六．雙向免疫擴散沉澱法（ Double Immunodiffusion ouchterlony ' s Immunodiffusion ) • • • • • 9 七．卵黃前質之定量一火箭式免疫電泳（ Rocke immunoelectrophoresis ) • • • • • • • • • • • • • 9 八．組織切片• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 10 九.粗脂肪分析• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 11 十. 極性脂肪及非極性脂肪分析．• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 12 十一.數據分析 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 12 參．結果 • • • • • • • • • • • • • • • • • • • • • 14 一．卵黃前質純化 • • • • • • • • • • • • • • • • • • • • • 14 二．以不同劑量的 HCG500 I.U ,1000 I.U ,2000I.U./Kg body weight , LHRH.a 20uglKg , LHRH-a 20 ug/Kg + Domperidone 5 mg/Kg , LHRH－a 200 mg / Kg pellet , LHRH－a 300 mg/Kg pellet ，注射或埋植方式處理另以藥丸埋植及生理食鹽水注射做為對照實驗，觀察誘導鰻魚卵成熟結果• • • • • • • • • • • 14 三．雌二醇處理對鰻魚卵巢成熟的影響 • • • • • • • • • • • • • • • • • • • • • • • 17 四．養殖鰻魚卵巢指數（Gonadosomatic index）、肝體比值(Hepatosomatic index）、卵黃前質的月變化 18 肆．討論• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 19 伍．參考文獻• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 25 陸．圖與表• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • 36 | |
| dc.language.iso | zh-TW | |
| dc.title | 白鰻催熟效果之比較研究 | zh_TW |
| dc.title | Evaluation on the induced maturation of Pacific eels , Anguilla japonica ,through endocrinological manipulation | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 77 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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