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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳麗光 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:09Z | - |
| dc.date.available | 2021-07-01T08:17:09Z | - |
| dc.date.issued | 1993 | |
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Major protein changes during vitellogenesis maturation of fundulus oocyles. Dev. Biol. 110,492—498. Watanabe, W. O. and Kuo, C. M. 1986. Water and ion balance in hydrating oocytes of the grey mullet,Mugil cephalus(L).. during hormone—induced final maturation.J. Fish Biol. 28, 425—437. Weinstein, S. P., Kostellow, A. B., Ziegler, D. H. and Morrill, G. A. 1982. Progesterone—induced down—regulation of an electrogenic Na+, K+ —ATPase during the first meiotic division in amphibian oocytes. J. Membr. Biol. 69.41—48. 陳英男， 1988 。香魚，（Plecoglossus alltivelis），卵細胞發育成熟過程中滲透壓調節之研究。國立臺灣大學漁業科學研究所碩士論文 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76002 | - |
| dc.description.abstract | 魚類卵細胞成熟過程中，當達到卵黃生成期（Vitellogenesis ) 後，即暫時停止發育，迄受到適當環境因數刺激，經由神經及內分泌調控，腦下垂體分泌促性腺激素（Gonadotropin , GtH）進而誘發類固醇生成作用（Steroidogenesis) ，並合成成熟誘導類固醇 (Maturation-Inducing Steroid , MIS ) ，即17α，20β-Dihydroxyprogesterone , 待卵細胞質內之成熟促進因數(Maturation -Promoting Factor , MPF ）被活化後，促使卵細胞進入最後成熟（Final maturation）。大多數魚種之卵細胞在最後成熟期間會有吸水（Hydration）的現象，此乃卵細胞滲透壓調節之結果。本研究以烏魚（Mugil cephalus）為材料，利用人類絨毛促性腺激素（Human chorionic gonadotropin , hCG）催熟，使其進入最後成熟，並依卵細胞型態特徵及卵核位置，將卵細胞分為卵核居中期（ Subperipheral stage , S ）、卵核移動期（Germinal vesicle migration , GVM ）、卵核破裂期（Germinal vesicle breakdown , GVBD）、排卵期（Ovulation , OV ）等四期，並以成熟卵細胞（即卵黃第三期，Tertiary globule stage , YG3）為對照組，測定最後成熟各階段中卵細胞內與血清中滲透壓的改變，同時並測量血清、卵細胞中Na+、K+、Ca+2、Mg+2及Cl- 五種離子之含量變化及卵中有機物、灰分、卵徑及水分含量，以瞭解吸水過程中，卵細胞生化組成之改變。結果發現：卵徑與水分含量隨最後成熟的進行，呈正相關的增加，而卵細胞滲透壓及其總陽離子含量亦有相同關係。此結果顯示，最後成熟期間卵細胞體積的增大為吸水之結果，而吸水現象的發生，乃卵細胞內大量離子進入以達到滲透壓調節所造成。而卵細胞中K+的大量增加，可能表示對烏魚而言，K+為影響吸水之重要因數。本研究亦測定最後成熟各階段的血清中，17β-estradiol (E2)及17α-hydroxyprogesterone (17α-OHP ) 之濃度變化。結果發現：二者之濃度皆隨最後成熟之進行而減少。而17α-hydroxyprogesterone 乃多種成熟誘導類固醇之前驅物，其濃度之降低，表示著成熟誘導類固醇分泌量之增加，以促動最後成熟的進行。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:09Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . . . .1 壹、前言. . . . . . . . . . . . .2 貳、材料與方法. . . . . . . . . . . . .8 一、實驗材料. . . . . . . . . . . . .8 （一）實驗用魚之來源及蓄育 （二）實驗用魚之採樣時期 （三）實驗樣品的收集與初步處理 二、實驗方法. . . . . . . . . . . . .11 （一）血清的分析項目及方法 （二）卵細胞的分析項目及方法 三、統計分析 . . . . . . . . . . . . .17 參、結果. . . . . . . . . . . . .18 一、血清及卵細胞中與滲透壓調節相關因數之變化. . . . . . . . . . . . .18 （一）血清及卵細胞之滲透壓 （二）卵細胞卵徑、水份、灰份及有機物含量 （三）血清及卵細胞中離子含量之變化 二、血清中類固醇濃度之變化. . . . . . . . . . . . .20 肆、討論. . . . . . . . . . . . .21 伍、參考文獻. . . . . . . . . . . . .27 陸、表. . . . . . . . . . . . .40 柒、圖. . . . . . . . . . . . .50 捌、附 錄. . . . . . . . . . . . .82 | |
| dc.language.iso | zh-TW | |
| dc.title | 烏魚卵細胞最後成熟過程之生理研究 | zh_TW |
| dc.title | PHYSIOLOGICAL PROCESSES OF GREY MULLET, Mugil cephalus L. DURING OOCYTE FINAL MATURATION | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 89 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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