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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 郭上卿 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:08Z | - |
| dc.date.available | 2021-07-01T08:17:08Z | - |
| dc.date.issued | 1977 | |
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Microbiol. 25(1) :155-156. 9 White, F. K., C. F Simpson. and L. E. Williams. (1973) Isolation of Edwardsiella tarda from aquatic animal species and surface water in Florida. J. Wild. Dis. 9:204-208. 10. Sonnenwirth, A. C and B. A. Kallus. (1968) Meningitis due to Edwardeiella tarda. Amer. J. Clin. Pathol. 49(1):92-95. 11. Bockem?hl, J., R. Pan-Urar. and F Burkhardt. (1971) Edwardsiella tarda associated with human disease. Path. Microbiol. 37:393-401. 12. Lewis, D. H. and T. C. Allison. (1971) An immunofluorescent technique for detacting Aeromonas liquefaciens in fish utilized in lunar exposure studies. Trans. Amer. Fish. Soc. 3:575-578. 13. Johnson, G. R. and G. Wobeser. (1974) Indirect fluorescent antibody technique for detection of RM bacterium of rainbow trout (Salmo gairdneri). J. Fish. Ras. Board. Can, 31:1957-1959. 14. Kitao, T. and M. Kimura, (1974) Rapid diagnosis pseudotuberculosis in yellowtail by means of the fluorescent antibody technique. Bull. Jap. Soc. Sci, Fish. 40(9):889-893. 15, Bullock, G. L. and H. M. Stuckey, (1975) Fluorescent antibody technique for rapid diagnosis of corynehacteria1 kidney disease. Fish Health News. 4(2) 16. Mori, M., T. Kitao. and M. Kimua (1976) A field survey by means of the direct fluorescent antibody technique for diagnosis of pseudotuberculosis in yellowtail. Fish Patho1oy, 11(1):11-16. 17. Shotts, E. B and R, Riuler, (1973) Medium for the isolation of Aeromonas hydrophila. Appl. Microbiol. 26(4):550-553. 18. Rhodes, M, E (1958) The cytology of Pseudomonas spp. as revealed by a silverp1ating staining method, J. Gen. Micrchiol. 18:639-648. 19. Mannual of microbiolgical methods (1957) Mc, Graw-Hall Book Company Inc New York. 20. Pacha, R, E. and S. Porter. (1968) Characteristics of Myxobacteria isolated from the surface of freehwater fish. Apple, Microbiol. 16(12):1901-1906. 21. Smith, H. L. and P. B. Fernandes. (1973) Modified decarboxylasedihydrolase medium, Appl, Microhiol. 26:620-621. 22, Porres, J. M, and R. E. Stanyon, (1974) One tube oxidation-fermentation test. Amer. J. Clin. Pathol. 45(4):1493-496. 23. Bauer, A, w., W. M. Kirby J. C, Sherris. and M Turck, (1966) Antibiotic suceptibi1ity testing by a standardized single disk method, Amer. J. Clin. Pathol. 45(4):493-496. 21. Campbell, D, H., F. S. Garvey., N. E. Cremer, and D. H, Sussdorf. (1970) Methods in immunology. W. A. Benjamin, Inc., Now York. 10016. 25. Burrell, R. and C. C. Mascoli, (1970) Experimental immur.ology 3rd ed, Burgess Publishing Company. p28. 26. Kawamura, A. J. (1969) Fluorescent antibody techniques and their applications, University Park Press, Baltimore, Ma. 27, Goldman, M, (1968) Fluorescent antibody methods, Academic Press Inc., Now York and London. 28. The, T. H. and T. H. W. Feltkamp. (1970) Conugation of fluorescein isothiocyanate to antibodies. IT A reproducible method J. Immunol. 18:875-881. 29. Pavlova, M. T., E, Beauvais., F. T. Brezeski. and. Litsky. (1972) Fluorescent antibody techniques for the identification of group D Streptococci: Direct staining method, Appl. Microbiol. 23(3):571-577. 30. Wakabayshi, H. and S, Egua. (1973) Edwardsiella tarda (Paracolo-bactrum anguillimortiferum) associatod with pond-cultured eel disease. Bull. Jap. Soc. Sci. Fish. 39(9):931-936. 31. Sakazaki, R and K. Tamura. (1975) Priority of the specific epithet anguillimortiferum over the specific epithet tarda in the name of the organism presently Known as Edwardsiella tarda. Internatl. J. Syst Bacteriol, 25(2):219-220. 32. 魚病診斷?針(1994) コイ、ウナギ、ハマナ承度水?序編，日本水?資源保護協會.156?55. 33. Aoki, T., S. Egusa, and T, Watanabe. (1972) Drug resistance and R factors in fish culturing. (In Japanese) Jap. J, bacteriol. 27:762-767. 34 Aoki, T. and T. Watanabe. (1973) Studies of drug-resitant bacteriaisolated from eel-pond water and intestinal tracts of the eel (Anilla japonica and Anguilla anguilla). (In Janpanese) Bull. Jap. Soc. Sci, Fish. 39(2):121-130. 35. Davis, B. D., R. Dulbecco., H, N, Eiaen., H. S. Ginsberg. and W. B. Wood. (1973) Microbiology. 2nd ed. Harper & Row, p771. 36. Sakazaki, R, (1967) Studies on the asakusa group of Enterobacteri-aceae (Edwardsieila tarda). Jap. J. Med. 3d. Biol, 20:203-212. 37 Hebert, G. A., P. L. Pelham. and B. Pittman. (1973) Determination of the optimal armonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. Appl. Microbiol. 25(1):26-36. 38. Klugerman, M. R. (1965) Chemical and physical variables affecting the properties of fluorescein isoth1otyanate and its protein conjugates, J. Immunol, 95:1165-1173. 39. Pital, A. and S. L, Janowitz. (1963) Enhancement of staining intensity inthesity in the fluorescent antibody reaction. J, Bacteriol. 86:888-889. 40. Clark, H. F. and C. C. Shepard. (1963) A dialysis technique for preparing fluorescent antibody, Virology. 20:642-644. 41. Beutner, E. H. (1961) Immunofluorescent staining: the fluorescent antibody method. Bact. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76001 | - |
| dc.description.abstract | 筆者於1975年4月至1976年6月間自本書各地罹患潰瘍病之病鰻?集至11株病原菌,其特?為革蘭氏陰性,大小為2-3μ x 1μ之短桿菌,周鞭毛,運動性極強,生化學特性為產生H2S,不產生Cytochrome oxidabe,僅對Glucose, Maltose, Fructose, Calactose, D-Mannose等醣類發酵利用,鑑定為Edward-siella anguillimortiferum。此11株病原菌均對Nalidixic acid, Nitrofurantoin及Kanamycin等三種藥物具有高感受性。 分別以標準法(Standard technique) 及透析法(Dialydis technipue) 將熒光物質(Fluorescein isothiocyanate, FITC) 標識抗E. anguillimortiferum之抗體，發現透析法中FITC與γ-globulin之摩爾比值較高，可得較高之染色體□數(Staining titer)。由11株分離菌，Aercmonas hydrophila, Vibrlo anguillarum及Pseudomonas spp,之直接熒光抗體染色之結果顯示抗E. anguillimortiferum之熒光抗體具有高專一性(Specilicity)。又11株分離菌之。抗原為同一種。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:08Z (GMT). No. of bitstreams: 0 Previous issue date: 1977 | en |
| dc.description.tableofcontents | 緒言…………………………………………………1 材料與方法…………………………………………5 一、Edwardsiella anguillimortiferum支分離………5 Ⅰ.病原性實驗………………………………………5 Ⅱ.病體之觀察………………………………………6 Ⅲ.生化學征性………………………………………6 Ⅳ.藥物感受試驗……………………………………7 二. Edwardsiella anguillimortiferum螢光測定………8 Ⅰ.細菌之培養………………………………………8 Ⅱ.抗原之製備………………………………………8 Ⅲ.抗體之製備………………………………………8 Ⅳ.血清球蛋白globulin(rg)之分離…………………9 Ⅴ.螢光物質之標識…………………………………9 Ⅵ.FITC與gam Globujin 比值之測定……………10 Ⅶ.螢光染色…………………………………………10 Ⅷ.螢光顯微鏡之觀察………………………………11 Ⅸ.專一性之測定……………………………………12 結果…………………………………………………14 討論…………………………………………………18 摘要…………………………………………………26 謝辭…………………………………………………27 參考文獻……………………………………………36 | |
| dc.language.iso | zh-TW | |
| dc.title | 養殖鰻潰瘍病病原菌Edwardsiella anguillimortiferum之分離及螢光診斷 | zh_TW |
| dc.date.schoolyear | 65-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 39 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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