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DC 欄位 | 值 | 語言 |
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dc.contributor.author | 曾淑汾 | zh_TW |
dc.date.accessioned | 2021-07-01T08:17:07Z | - |
dc.date.available | 2021-07-01T08:17:07Z | - |
dc.date.issued | 1993 | |
dc.identifier.citation | Abramovitz,M., H. Homma, S. Ishigaki, F. Tansey, W. Cammer, and I. Listowsky (1988) Characterization and localization of glutathione-S-transferases in rat brain and binding of hormones, neurotransmitters, and drugs.J.Neurochern., 50:50-57.
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Pyerin (1989) Glutathione S-transferase is an in vitro substrate of Ca++ -phospholipid-dependent protein kinase (protein kinase C). Biochem. Biophys. Res. Commun., 162:903-907. Thomas, D.B. (1991) Role of glutathione S-transferases in heme transport. Biochem. Pharmacol., 42: 188-190. Toung, Y.S., T.S. Hsieh, and C.P. Tu (1990) Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase . Proc. Natl. Acad. Sci. USA, 87:31-35. Tsao, B., and N.P. Curthoys (1980) The absolute asymmetry of orientathion of r-glutamyltranspeptidase and aminopeptidase of the external surface of the rat renal brush border membrane. J. Biol. Chem., 255:7708-7711. Tu, C.P., M.J. Weiss, N.Q. Li, and C.C. Redd (1983) Tissue-specific expression of the rat glutathione S-transferase. J. Biol. Chem., 258:4659- 4662. Vorachek, W.R., W.R. Pearson, G.S. Rule (1991) Cloning, expression, and characterization of a class-mu glutathione transferase from human muscle, the product of GST4 locus. Proc. Natl. Acad. Sci. USA, 88:4443-4447. Wolf, M., H. LeVine, S. May, P. Cuatrecasas, and N. Sahyoun (1985) A model for intracellular translocation of protein kinase C involving synergism between Ca++ and phorbol esters. Nature, 317:546-549. Saneto, R.P., Y.C. Awasthi, and S.K. Srivastava (1982) Glutathione S-transferases of the bovine retina, Evidence that glutathione peroxidase activity is the result of glutathione S-transferase. Biochem. J., 205:213-217. Sassa, T. and J. Miwa (1992) Purification and characterization of protein kinase C from the nematode Caenorhabditis elegans. Biochem. J., 282:219-223. Seidegard, J., W.R. Vorachek, R.W. Pero, and W.R. Pearson (1988) Hereditary differences in the expression of the human glutathione transferase active on trans-stilbene oxide are due to a gene deletion. Proc. Natl. Acad. Sci. USA, 85:7293-7297. Sies, H., and P. Graf (1985) Hepatic thiol and glutathione efflux under the influence of vasopressin, phenylephrine and adrenaline. Biochem. J., 226: 545-549. Staffas, L., L. Mankowitz, M. Sodderstrom, A. Blanck, I. Porsch-Hallstrom, C. Sundberg, B. Mannervik, B. Olin, J. Pydstrom, and J.W. DePierre (1992) Further characterization of hormonal regulation of glutathione transferase in rat liver and adrenal glands. Biochem. J., 286:65-72. Tahir, M.K., C. Guthenberg, and B. Mannervik (1985) Inhibitors for distinction of three types of human glutathione transferase. FEBS Lett., 181:219-252. Tan, K.H., V.J. Meyer, J. Be1in, and B. Ketterer (1984) Inhibition of microsomal lipid peroxidation by glutathione and glutathione transferases B and AA, role of endogenous phospholipase A2. Biochem. J., 220:243-252. Tan, K.H., D.J. Meyer, B. Coles, and B. Ketterer (1986) Thymine hydroperoxide, a substrate for rat Se-dependent glutathione peroxidase and glutathione transferase isoenzymes. FEBS Lett., 207:231-233. Tan, K.H., D.J. Meyer, N. Gillies, and B. Ketterer (1988) Detoxification of DNA hydroperoxide by glutathione transferases and the purification andcharacterization of glutathione transferases of the rat liver nucleus. Biochem. J., 254:841-845. Taniguchi, M., and M.G. Cherian (1990) Ontogenic changes in hepatic glutathione and metallothionein in rats and the effect of a low-sulphur-containing diet. Briti. J. Nutri., 63:97-103. Taniguchi. H. and W. Pyerin (1989) Glutathione S-transferase is an in vitro substrate of Ca++ -phospholipid-dependent protein kinase (protein kinase C). Biochem. Biophys. Res. Commun., 162:903-907. Thomas, D.B. (1991) Role of glutathione S-transferases in heme transport. Biochem. Pharmacol., 42: 188-190. Toung, Y.S., T.S. Hsieh, and C.P. Tu (1990) Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase . Proc. Natl. Acad. Sci. USA, 87:31-35. Tsao, B., and N.P. Curthoys (1980) The absolute asymmetry of orientathion of r-glutamyltranspeptidase and aminopeptidase of the external surface of the rat renal brush border membrane. J. Biol. Chem., 255:7708-7711. Tu, C.P., M.J. Weiss, N.Q. Li, and C.C. Redd (1983) Tissue-specific expression of the rat glutathione S-transferase. J. Biol. Chem., 258:4659- 4662. Vorachek, W.R., W.R. Pearson, G.S. Rule (1991) Cloning, expression, and characterization of a class-mu glutathione transferase from human muscle, the product of GST4 locus. Proc. Natl. Acad. Sci. USA, 88:4443-4447. Wolf, M., H. LeVine, S. May, P. Cuatrecasas, and N. Sahyoun (1985) A model for intracellular translocation of protein kinase C involving synergism between Ca++ and phorbol esters. Nature, 317:546-549. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75998 | - |
dc.description.abstract | 用S-hexylglutathione吸附性管膠與陰離子交換管膠Mono Q可將斑節蝦眼睛的GST θ和GST ?分離。比較GSTθ與GST ?的相對含量以眼睛和鰓最高,神經次之。根據SDS-PAGE、SuperoseTM-12的証據,顯示GST θ為二聚體(Diner),分子量約為50KDa,能與Corticosterone結合。經SDS-PAGE分析可結合在單元體(monomer)上,並能以10 mM 的Glutathione影響GSTθ與Corticosterone間的結合。老鼠的蛋白質激?C(PKC)、可使斑節蝦GSTθ進行磷酸化,但是,斑節蝦的蛋白質激?C無法使斑節蝦的GST磷酸化。 | zh_TW |
dc.description.abstract | Two anionic isoenzymes of glutathione S transferase (EC 2.5.1.18), GSTθand GST ?,have been purified from the eyes of the shrimp Penaeus japonicus by using a combination of S-hexylglutathione affinity column chronmatography and Mono Q fast protein liquid chromatography (f.p.l.c.). The purified GST θ was a dimeric enzyme protein with subunit Mr abount 25 kDa. HPLC reverse phase C4 column analysis of various shrimp tissues indicated that the ratio of GSTθ vs. GST ? was highest in the eye and gi11. HPLC reverse phase C4 column analysis demostrated that corticosterone was selective binding to GST θ. Glutathione influenced the binding of corticosterone to the GST θ. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:07Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
dc.description.tableofcontents | 表目次………………………………………………………………………………………………Ⅳ 圖目次………………………………………………………………………………………………Ⅴ 縮寫表………………………………………………………………………………………………Ⅵ 試藥…………………………………………………………………………………………………Ⅶ 中文摘要……………………………………………………………………………………………1 英文摘要……………………………………………………………………………………………2 一、緒言……………………………………………………………………………………………3 二、方法……………………………………………………………………………………………6 1.酵素純化 ……………………………………………………………………………………6 2. 硫酸十二酯鈉聚丙烯醯胺膠體電泳(SDS-PAGE )分析……………………………………11 3.膠體過瀘層析法 ……………………………………………………………………………12 4.交聯(cross link )反應……………………………………………………………………12 5.放射線分析 …………………………………………………………………………………13 三、結果……………………………………………………………………………………………14 四、討論……………………………………………………………………………………………18 五、參考文獻………………………………………………………………………………………22 六、圖表……………………………………………………………………………………………3l | |
dc.language.iso | zh-TW | |
dc.title | 斑節蝦眼中穀胱甘?轉換?θ與皮質酮結合的探討 | zh_TW |
dc.title | The binding of corticosterone with glutathione S-transferaseθpurified from the eyes of kuruma shrimp, Penaeus japonicus. | en |
dc.date.schoolyear | 81-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 65 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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