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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75986完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 李宗徽 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:00Z | - |
| dc.date.available | 2021-07-01T08:17:00Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75986 | - |
| dc.description.abstract | 甲殼類之蛻殼機制主要是由眼柄中之x-organ分泌之勝?類的蛻殼抑制激素（MIH）來控制；但根據最近的研究報告指出，藍蟹和螯蝦眼柄中另含xanthurenic acid(XA）和3－OH－kynurenine(3-OH-K)二種 基酸代謝物質，對其蝦、蟹體本身亦具有蛻殼上之抑制作用。在台灣諸多甲殼類之生理研究上，相關於蛻殼抑制激素的研究極為有限，因此就本省幾種養殖之對蝦及秦國沼蝦為材料，利用高效液相層析建立一套標準化的定性、定量的分析系統，分析其眼柄中是否含有類似的物質存在，在初步的鑑定程式中，並佐以氣相層析與質譜之分析，肯定該等物質之存在。 在完成之高效層析系統建立後，於不同蛻殼週期下之泰國沼蝦眼柄中分析其XA和3-OH-K之含量，比較其間之互動關係。另建立類似之逆相高效層析以分離偵檢其血淋巴內之蛻殼激素（ecdysteroids）的含量並配合週期之變化分析激素含量的變化。本研究所建立之定性、定量高效層析系統，經審慎評估可精確偵檢到單隻蝦體內血淋巴或眼柄中，此兩類不同激素之含量。而該項分析系統之確立，應用於測定不同蛻殼週期中，蛻殼激素與蛻殼抑制激素含量之變化與相關情形，以確認甲殼類蛻殼機制中之化學控制因數。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:00Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 一、前言………………………………………1 二、材料與方法………………………………………6 I 、藥品來源與重要儀器…………………………6 II、草蝦眼柄萃出物中黃尿醯酸的純化與鑑定…………7 III、不同蝦種眼柄萃出物之高效層析樣品的制備與偵檢……9 IV、泰國沼蝦於蝦殼週期間眼柄蛻殼抑制激素和血淋巴蛻殼激素分析…10 V 、沼蝦眼柄萃出物之遊離胺基酸分析…………………12 三、結果………………………………………………………………13 I、黃尿醯酸、3－OH－犬尿素高效層析系統的建立……………13 II、草蝦眼柄中黃尿醯酸的純化與鑑定………………………14 III、不同蝦種眼柄中黃尿醯酸、3－OH－犬尿素之分析……14 IV 、沼蝦蛻殼週期中黃尿醯酸、3－OH－犬尿素與β－型蛻殼激素之含量分析……15 V 、單只蝦眼柄萃出物遊離胺基酸之分析……………………………19 四、討論…………………………………20 五、參考文獻………………………25 六、附表………………………33 七、附圖……………………34 八、附錄…………………60 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣養殖蝦類蛻?抑制因數及蛻?激素之分析研究 | zh_TW |
| dc.title | Studies on the analysis of molting-inhibiting related tryptophan metabolites and ecdysones in shrimps of Taiwan | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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