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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 李至剛 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:54Z | - |
| dc.date.available | 2021-07-01T08:16:54Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75973 | - |
| dc.description.abstract | 本實驗對鑲點石班（Epinephelus amblycephalus）的腦及眼球色素細胞進行初級培養，培養基成份為L-15及胎牛血清。在31℃，血清濃度20％，添加3g／L NaCl條件下培養。實驗記錄了培養過程中細胞的特性，並測試兩株細胞對病毒感受性及腦細胞最適當的培養條件。以IPNV-Ab、Herpes、Reo Virus三種病毒測試的結果，兩株都對IPNV及Reo Virus具感受性，對Herpes均無。至於最適生長條件：腦細胞為31℃，血清濃度20％，添加0-8g／L的鹽。細胞倍增週期介於2?3天之間。目前眼球細胞已繼代到第八代，腦細胞部份繼代到第四代，均已冷凍保存於液態氮中。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 摘要……………………………………I 謝辭……………………………………II 目錄……………………………………IV 第一章 緒論……………………………………1 第二章 材料和方法……………………………………5 第一節 實驗材料……………………………………5 （一）實驗用石斑魚……………………………………5 （二）實驗使用的溶液配方……………………………………5 第二節 實驗方法……………………………………9 （一）初級培養……………………………………9 （二）繼代培養……………………………………9 （三）血清濃度對細胞生長的影響……………………………………10 （四）滲透壓對細胞生長的影響……………………………………10 （五）溫度對細胞生長的影響……………………………………11 （六）對各種病毒的感受性……………………………………11 （七）釋放病毒的力價……………………………………13 （八）光學及電子顯微鏡觀察……………………………………13 （九）污染測試……………………………………14 （十）組織切片……………………………………15 第三章 結果……………………………………16 （一）初級培養……………………………………16 （二）繼代培養……………………………………17 （三）血清濃度對細胞生長的影響……………………………………18 （四）滲透壓對細胞生長的影響……………………………………18 （五）溫度對細胞生長的影響……………………………………18 （六）對各種病毒的感受性……………………………………18 （七）釋放病毒的力價……………………………………19 （八）光學及電子顯微鏡觀察……………………………………19 （九）污染測試……………………………………20 （十）組織切片……………………………………20 第四章 討論……………………………………22 參考文獻……………………………………27 圖表……………………………………34 | |
| dc.language.iso | zh-TW | |
| dc.title | 鑲點石斑（Epinephelus amblycephalus）眼球色素細胞與腦細胞之培養及其特性研究 | zh_TW |
| dc.title | The Establishment and Characterization of Two Primary Culture Derived from Ophthalmic Pigment Cell and Brain of Epinephelus amblycephalus | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 60 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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