請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75968完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | C.H.WONG | en |
| dc.contributor.author | 翁?惠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:52Z | - |
| dc.date.available | 2021-07-01T08:16:52Z | - |
| dc.date.issued | 1977 | |
| dc.identifier.citation | 1.a) K. Hayaski, M. Takechi, T. Sasaki and C. Y. Lee, Biochem, Biophys. Res, Commun,, 64, 360 (1975), b) M. Takechi, T. Sasaki, N, Kaneda and K. Hayashi, FEBS Lett,, 66, 210 (1976), c) N. Kaneda, T. Sasaki and K, Hayashi, Biochem. Biophys. Res, Commun., 72, 1450(1976). d) N, Kaneda, T, Sasaki and K, Hayashi, FEBS Lett,, 70, 217 (1976) 2. K. Narita and C, Y. Lee, Biochem, Biophys, Res, Commun, 41, 339 (1970) 3. C. Y. Lee, A, Rev, Pharmac.,, 12, 265 (1972) 4 K. L. Cheng and T. B, Lo, unpublished, 5 H, Bodanszky, V. du vigneaud, Nature, 183, 1324 (1954) 6 R, Schwyzer, P. Sieber, Helv. Chim Acta,, 49, 1311 (1966) 7 R. Garner, G. T. Young, J Chem. Soc,, D50 (1971) 8 A. V. Zon and H. C, Beyerman, Helv, Chim. Acta,, 59, 1112 (1976). 9. P. Sieber, B, Kamber, A, Hartmann, A. Johl, B. Riniker and W. Rittel, Helv, Chim, Acta,,57 , 2617 (1974) 10. R.B. Merrifield, J, Am, Chem, Soc,, 85, 2149 (1963) 11 R. B. Merrifield, J, Am, Chem, Soc,, 86, 304 (1964) 12. G. R. Marshall, R. B. Merrifield, Biochemistry, 4, 2394 (1965) 13 M Manning, J, Am, Chem Soc, 90, 1348 (1968) 14 D, Yamashiro and C,,H, Li, J, Am, Chem, Soc,, 95, 1310 (1973) 15. N. H, Tan and E. T. Kaiser, J, Org. Chem,, 41, 2787 (1976) 16. A. Haruhiko, H. Yonezawa, N Takahashi, N, Izumiya and C. C, Yang,,,Biochim Biophys. Acta,, 263, 823 (1972) 17. J, J. Sharp, A, B, Ribinson and M. D. Jamen, J, Am, Chem, Soc., 95, 6097 (1973) 18. B, Gutte, R. B. Merrifield, J. Biol. Chem,, 246, 1922 (1971) 19. H. Yajima, Y. Kiso, Y, Okada, and H. Watanabe, J.S.C. Chem, Comm,, 106 (1974) 20.R. Matsueda, H. Maruyama, E. Kitazawa., H. Takahagi, and T. Mukaiyama, Bull, Chem Soc, Jpn., 46, 3240 (1973) 21. H, Determann, J. Heuer, P. Pfaender, and M. L. Reinartz, Ann,, 694, 190 (1966) 22. J. C, Sheehan, M, Goodman and G. P. Hess, J. Am, Chem, Soc,,78, 1367 (1956) 23. M. W Williams and G. T, Young, J. Chem, Soc,, C3701 (1964) 24. W Konig and R. Geiger, Chem, Ber,, 103, 788 (1970) 25.J, Biol, Chem,,7, 977 (1971) 26. T. B. Lo, Y. H, Chen and C.Y. Lee, J, Chinese Chem, Soc,, 13, 25 (1966) 27. M, C, Houng and Y, H. Chen, Internat. J, Peptide and Protein Res.,, in Press, 28 .R, Nietzki and E, Braunschweigh, Ber,, 27, 3381 (1894) 29.a)H, Gilman, A, H, Blatt, Org. Syn., Collective Vo,1, 220 (1951) b) A, H, Blatt, Org, Syn,, Collective Vo..2, 455 (1955) 30. R, A, Reisfeld, U, J. Lewis, D. E, Williams, Nature 195, 281 (1962) 31.K. R, Woods and K, T. Wang, Biochim, Biophys, Acta, 133, 369 (1967) 32. H, N, Rydon, P. W, G, Smith, Nature, 169, 922 (1952) 33. D. H, Spackman, S, Moor and W. H. Stein, Anal, Chem, 30, 1190 (1958) 34. F. C, Westall, J, Scotchier and A. B. Robinson, J, Org. Chem,, 37, 3363 (1972) 35. G. L, Ellman, Arch, Biochem Biophys,,, 82, 70 (1959) 36. a) L. .Zervas, D, Borouas and E, Gazis, J, Am, Chem Soc,, 85, 3660 (1963) b) L. Zervas and C. Hamalidis, J, Am Chem, Soc,, 87(1), 99 (1965) 37. a) B, Schnabel, Liebigs Ann, Chem,, 702, 188 (1967) b) K. P. Polyhofer, Tetrakedron Lett,, 27, 2305 (1969) 38. S. Guttmann and ,R, A. Boissonnas, Helv, Chim, Acta,, 41, 1852 (1958) 39. a) V. J. Hruby and K. W. Ehier, J. Org. Chem,, 35, 1960 (1970), K, Okawa, Bull, Chem Soc, Jpn,, 30, 110 (1957) b) T. Mizoguchi, G. Levin, D, W. Wooley and J. M. Stewart, J, Org. Chem., 33, 903 (1968). c) J. S. Morley, J, Chem. Soc,, C2410 (1967) 40. V.du Vigneaud, L, F, Audrieth and H. S.Loring, Am Chem Soc.., 52, 4500 (1930) 41. B. F, Erlanger, W. V. Curran and N, Kokowsky, J, Am,, Chem, Soc,52, 81 3051 (1959) 42.J. Ramachandran and C, H, Li, J. Org. Chem,,27, 4006 (1962) H. Oksuka and K, Inouye, Bull, Chem, Soc Jpn,, 37(10), 1465 (1964) 43. R, A, Biossinnas, S. Guttmann, R, L. Huguenin, P. A, Jaquenod and E.Sandrin, Helv, Chim, Acta,,, 41, 1867 (1958) 44. J. A. Waclaren, Aut, J, Chem,, 25, 1293 (1972) 45. a) C, H. Wong, M. H. Hsiao and K, T. Wang, J, Chinese Biochem, Soc., 2(2), 64 (1973). b) C, H. Wong and K, T, Wang J, Chinese Biochem, Sac,, 4, 24 (1975) 46. A, Marglin, Tetrakedron Lett,, 33, 3145 (1971) 47.E, Kaiser, R, L, Colescott, C. D, Bossinger and P. I. Cook, Anal, Biochem,, 34, 595 (1970) 48. S. Sakakibara, Y. Shimonishi, Y. Kishida, M, Okada, and Sugihara, Bull, Chem, Soc Jpn.,, 40, 2164 (1967) 49. M. Z. Atassi, A. F. S. A. Habeeb and L. Rydstedt, Biochim, Biophys, Acta, 184 (1970) 50. P, Cuatrecasas, J, Biol, Chem,, 245, 3059 (1970) 51. O. H, Lowry, N, J. Rosebrough, A, L. Farr, and R. J. Randall, J, Biol, Chem,, 193, 265 (1951) 52. J. T, Litchfield, Jr, and F, Wilcoxon, J, Pharmac, Exp, Ther,,, 96, 99. (1949) 53. O. Ouchterlong, Progress in Allergy, 6, 30 (1962) 54. N. H. Tan and E. P. Kaiser, J. Org. Chem,, 41, 2787 (1976) 55. D. Kowaiski and N. Caskowski, Jr., Biochemistry 15, 1300 (1976) 56. S. Bauer, R. Lamed, and Y. Lapidot, Biotech Bioeng.,14 , 861 (1972) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75968 | - |
| dc.description.abstract | 台灣飯匙倩蛇毒心藏毒由60個氨基酸所構成，其中含四對雙硫鍵。為瞭解構造與生物活性的關係，首先以化學合成方法加以探討。 經由逐步固相合成及片斷固相合成所得產物以液態氟化氫除去所有保護基，然後以分子篩過濾分離出還原態心臟毒。再將其置於適當水溶液中令其自動氧化並進行立體特異組合而成氧化態心臟毒。粗產物經分子篩過瀘，陽離子交換，及親和柱層分離重複純化，精製而成人工合成心臟毒。最後收量以片斷固相合成法較高（0.5%)，且較容易純化。 人工合成心臟毒其氨基酸組成分析，電泳分析，薄層色層分析及 CD -光譜分析發現與天然心臟毒相同。免疫活性，頸肌收縮活性及毒性試驗也與天然所表現相同（約90%)。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1977 | en |
| dc.description.tableofcontents | I．Intruduction…………………………………………………………………………1 I-1 Structure and function of cardiotoxin………………………………………………1 I-2 Propose of the chemical synthesis………………………………………………………1 I-3 Stwategy and tactics of the synthesis…………………………………………………1 II. Experimental methods……………………………………………………………………9 II-1.Symbols of abbreviations and the materials used……………………………………9 II-2 Physical, chemical characterization of the synthetic product…………………10 II-3 Preparation of amino acid derivative……………………………………………12 II-4 Preparation of peptide fragment…………………………………………………12 II-4-1 DCC-method………………………………………………………………………12 II-4-2 DCC-HONSu method……………………………………………………………13 II-4-3 DCC-HOBt method……………………………………………………………13 II-4-4 Active ester method…..…………………………………………………………13 II-5 Attachment of C-terminal amino acid to resin…………………………………14 II-6 Stepwise solid phase synthesis of protected polypeptide……………………15 II-7 Fragment solid phase synthesis of protected peptide…………………………15 II-8 Liquid HF cleavage…………………………………………………………………16 II-9 Refolding of reduced natural CTX………………………………………………17 II-10 Purification of the synthetic CTX in a reduced state the folding……………17 II-10-1 For the fragment solid phase synthesis………………………………………17 II-10-2 For the stepwise solid phase synthesis………………………………………18 II-11 Purification of the synthesized CTX after oxidation………………………….18 II_12Preparation of anti-CTX sera and immunoadsorbant………………………..18 II-13 The bioassay……………………………19 III.RESULTS..........................................................39 III-1 Preparation of protected amino acid and peptide fragment for solid phase synthesis…………39 III-2 Refolding of reduced natural cardiotoxin………………………………39 III-3 The stepwise solid phase synthesis of cardiotoxin ………………40 III-4 The fragment solid phase synthesis of cardiotoxin …………………41 IV. Discussion………………………………62 V. Acknowledgement…………………63 VI References…………………………64 | |
| dc.language.iso | zh-TW | |
| dc.title | 台灣飯匙倩蛇毒心?毒之化學合成 | zh_TW |
| dc.title | TOTAL SYNTHESIS OF TAIWAN COBRA CARDIOTOXIN | en |
| dc.date.schoolyear | 65-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。