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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 黃曉薇 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:51Z | - |
| dc.date.available | 2021-07-01T08:16:51Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75967 | - |
| dc.description.abstract | 經鯉魚母系基因轉殖之人類腦下腺腫瘤細胞（Hp-3），與親代細胞（Hpt-1）相比較，已不能在半固態培養基內生長。免疫沈澱及西方點墨分析的結果則顯示，其EGFR之蛋白質量明顯地降低，但GFAP及p53蛋白質則增加。有趣的是，Hp-3細胞對細菌級培養皿的附著情形遠高於Hpt-1細胞，故二種細胞所分泌之細胞外間質，應有極大的差異。 Hpt-1及Hp-3細胞的條件培養液中，含有一活性，可以刺激BL、Hela、NIH 3T3及人類纖維細胞的生長，然而對Hpt-1及Hp-3細胞本身的效果則不顯著。且二細胞條件培養液之刺激生長活性並無明顯差異。以Hela及3T3細胞為主要的分析系統，則發現隨著條件培養液濃度的升高，細胞之增殖亦隨之增加。以0.5 ug/ml anti-bFGF antibody處理條件培養液，並未中和此活性，因而推測此活性應與bFGF無關。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:51Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 壹．中文摘要……………………………………………………I 貳．謝辭……………………………………………………II 參．中文內容 一、緒言……………………………………………………1 二、材料與方法……………………………………………………4 （一）細胞培養……………………………………………………4 （二）質體轉殖……………………………………………………4 （三）DNA雜合反應……………………………………………………4 （四）RNA雜合反應……………………………………………………5 （五）不同條件下細胞的生長……………………………………………………5 （六）致癌能力分析……………………………………………………5 （七）西方點墨分析……………………………………………………5 （八）細胞免疫染色……………………………………………………6 （九）免疫沉澱……………………………………………………6 （十）條件培養液的製備……………………………………………………6 （十一）濃縮條件培養液……………………………………………………6 （十二）刺激細胞生長活性之分析……………………………………………………7 三、結果……………………………………………………8 （一）母系基因之轉殖……………………………………………………8 （二）Hpt-1及Hp-3細胞的生長模式……………………………………………………8 （三）細胞在細菌級培養皿上生長的比較……………………………………………………9 （四）Hpt-1及Hp-3細胞基因表現的不同……………………………………………………9 （五）存於Hpt-1及Hp-3條件培養液內之促進生長活性……………………………………………………9 （六）條件培養液內之生長因數並非bFGF……………………………………………………10 四、討論……………………………………………………11 肆．英文內容……………………………………………………13 | |
| dc.language.iso | zh-TW | |
| dc.title | 人類腦下腺腫瘤細胞株經鯉魚母系基因轉型後其生長及分化之研究 | zh_TW |
| dc.title | Growth and Differentiation of Carp Maternal Gene Transformed Human Pituitary Gland Tumor Cells | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 60 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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