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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 劉景瑞 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:37Z | - |
| dc.date.available | 2021-07-01T08:16:37Z | - |
| dc.date.issued | 1993 | |
| dc.identifier.citation | Albers. J., Brunzell, J. D. and Knopp. R. H. (1989). Apoprotein measurements and their clinical applicaton. Clin. Lab. Med. Vol.9, p.137-152 Allen, W. V. (1972). Lipid transport in the dungeness carb. Cancer magister Dana. Comp. Biochem. Physiol. Vol. 43B, p.193-207 Anthony. H. M.. Terpstra. F. J., Sanchez-Muniz. C. E. W. and Christopher. J. H. W. (1982). The density profile and cholesterol concentration of serum lipoprotenins in domestic and laboratory animals, Comp. Biochem. Physiol. Vol. 71B, p.669-763 Babin, P. J. (1987). Apolipoproteins and the association of egg yolk proteins with plasma high density lipoproteins after ovulation and follicular atresia in the rainbow trout (Salmo gairdneri). J. Biol. Chem. Vol. 262, p.4290-4294 Babin . P. J. and Vernier. J. (1989). Plasma lipoproteins in Fish. J. Lipid Res. Vol.30. p.467-489 Bergink, E. W., Wallace. R. A. (1974). 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Studies on carotenoid-containing lipoproteins isolated from the eggs and ovaries of certain marine invertebrates. Comp. Biochem. Physiol. Vol. 22, p.851-871 李武忠(1987)淡水長臂大蝦養殖。養蝦總覽，p.280-283 養魚世界雜誌社出版 廖一久，趙乃賢，曾金菊，潘麗雪(1983)．淡水長臂大蝦專輯。臺灣省水產試驗所東港分所 小笠原義光(1987)．蝦的生態。養蝦總覽，p.377-413養魚世界雜誌社出版 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75940 | - |
| dc.description.abstract | 卵黃前質(vitellogenin)及卵黃質(vitellin)是含脂質之大分子，本研究構想由脂蛋白質(lipoprotein)之觀點來瞭解卵黃生成作用(vitellogensis)及脂蛋白質在卵中之堆積。故以淡水長臂大蝦(Macrobrachium rsenbergii)為材料，進行血淋巴、卵巢及卵中脂蛋白質之分離研究。 淡水長臂大蝦為抱卵蝦類，抱卵的顏色會隨胚胎發育而改變，由發育初期的鵝黃色漸轉為橘紅、黃灰，最後變為黑褐色。本實驗腹部抱卵而同時卵巢亦發育者，其卵的顏色和卵巢發育程度之關係為：抱鵝黃色卵者，其平均GSI為0.69±0.21%、橘紅色者為1.03±0.31%、黃灰色者為3.17±1.31%、黑褐色者為4.53±1.34%，即卵巢成熟的程度有隨抱卵顏色的加深而增大的現象。 以電泳分離血淋巴、卵巢及卵中脂蛋白質，並以梯度密度超高速離心的方法決定脂蛋白質之密度，得到以下結果： (1)淡水長臂大蝦在卵巢於發育及不發育時期，血淋巴中一直存在一種密度為1.089之脂蛋白質。卵巢於發育時期，血淋巴會增加三種脂蛋白質，一為母蝦所獨有，認定為卵黃前質，密度為1.172，其出現時間比另兩種脂蛋白質慢；另兩種脂蛋白質密度在1.041-1.103之間。 (2)卵巢成熟過程中，卵巢內出現三種主要的蛋白質，其中一種為密度1.193之脂蛋白質，含量隨卵巢成熟而逐漸增加，在成熟卵巢中約為未成熟者的20倍之多。 (3)鵝黃色、橘紅色及黃灰色卵內一直存在一種密度為1.190之脂蛋白質，其吸收光譜和卵巢內脂蛋白質相同，但兩者chloroform在萃取液吸收光譜略有不同，故推測兩者為極相近之物質，其含量在鵝黃色及橘紅色卵內無顯著差異，但隨卵發育至顏色轉為黃灰色時，含量則明顯下降百分之六十之多。黑褐色卵中可發現五種脂蛋白質，此現象顯示有多種新蛋白質合成出並以脂蛋白質型式存在，足見脂蛋白質在即將孵化之幼苗的組織構成佔有相當的地位，其間脂質如何轉變則有待進一步探討。 以chloroform/methanol/distilled water(2:2:l)萃取類胡蘿蔔素(carotenoids)並以最大吸光值進行定量。其結果：卵巢發育過程中，卵巢內脂蛋白質所攜帶之類胡蘿蔔素其含量有逐漸升高的趨勢，已成熟者約為未成熟者的10倍之多，而卵巢類胡蘿蔔素含量約增高3倍之多。在卵內，其脂蛋白質所攜帶之類胡蘿蔔素含量於卵發育期間有逐漸增加再下降的情況，由鵝黃色的0.43±0.31(mg carotenoids/Kg lipoprotein)，上升至橘紅色的0.58±0.24，再明顯下降至黃灰色的0.17±0.91，而卵之類胡蘿蔔素含量，鵝黃色為2.80±1.09(mg carotenoids/1009 egg)，橘紅色上升為6.18±1.08，至黃灰色下降為3.80±1.28，黑褐色為4.00±0.81。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:37Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 摘要…………………………………………1 壹、前言…………………………………………2 貳、材料及方法…………………………………………5 參、結果…………………………………………9 肆、討論…………………………………………14 伍、參考文獻…………………………………………22 陸、圖表…………………………………………28 | |
| dc.language.iso | zh-TW | |
| dc.title | 淡水長臂大抱卵期中脂蛋白質變動之研究 | zh_TW |
| dc.title | Viariation in lipoprotein of the matuting giant freshwater prawn,Macrobrachium rosenbergii during brooding peroid | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 56 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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