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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 鄭俊明 | zh_TW |
dc.date.accessioned | 2021-07-01T08:16:32Z | - |
dc.date.available | 2021-07-01T08:16:32Z | - |
dc.date.issued | 1992 | |
dc.identifier.citation | 張國廷, 1986 。紫菜絲狀體之化學分析,國立臺灣大學海洋研究所碩士論文。 尾行英二, 1970 。新しぃ海藻培養液SWM-IIIこぃ。Bull . Jap . Soc . phyco . 18 : 171-173。 林南增, 1989 。高效液相層析法概要,昇陽出版社, p . 211-240 。 Bennett, A. and L. Bogorad, 1971, Properties of subunits and aggregates of blue-green algal biliproteins. Biochemistry 10: 3625-3634. Bennett, A. and L. Bogorad, 1973, Complementary chromatic adaptation in a filamentous blue-green alga. J. Cell Biol. 58: 419-435. Berns, D. S., 1967, Imnunochemistry of biliproteins. Plant Physiol. 42: 1469-1586. Berns, D. S., 1971, Phycocyanins and deuterated proteins. Biology of Macromolecules. 5: 105-148. Bogorad, L., 1975, Phycobiliproteins and complementary chromatic adaptation. Ann. Rev. Plant Physiol. 26: 369-401. Boney, A. D. and E. B. White, 1968, Phycoerythrin from some Acrochaetium species. Nature 218: 1068-1069. Brown, A. S., J. A. Foster, P. V. Voynow, C. Franzblau and R. F. Troxler, 1975, Allophycocyanin from the filamentous cyanophyte, Phormidium luridum. Biochemistry16: 3581-3588. Chapman, D. J., W. J. Cole and H. W. Siegelman, 1968a, A comparative study of the phycoerythrin chromophore. Phytochem. 7: 1831-1835. Corran, P. H., 1989, Reversed-phase chromatography of proteins. In: HPLC of Macromolecules: a Practical Approach, R. W. A. Oliver, Ed. IRL Press, New York, pp.127-157. Dougherty, E. C. and M. B. Allen, 1970, Is pigmentation a clue to protistan phylogeny In: Biochemistry of Photoreceptive Systems, M. B. Allen, Ed. Academic Press, New York, pp. 129-144. Duerring, M., G. B. Schmidt and R. Huber, 1991, Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66A resolution. J. Mol. Biol. 217:577-592. Dunlap III , C. E., S. Gentleman and L. I. Lowney, 1978, Use of trifluoroacetic acid in the seperation of opiates and opioid peptides by reversed-phase high-performance liquid chromatography. J. chromatogr. 160: 191-198. Fisher, R. G., N. E. Woods, H. E. Fuchs and R. M. Sweet, 1980, Three-dimensional structures of c-phycocyanin and B-phycoerythrin at 5A resolution. J. Biol. Chem. 255:5082-5089. Frank, G., W. W. Sidler, H. Z. Widmer, 1978, The complete amino acid sequence of both subunits of C-Phycocyanin from the cyanobacterium Mastigocladus laminosus. Hoppe-Seyler. Z. Physiol. Chem. 359: 1491-1507. Gantt, E., 1969, Properties and ultrastructure of phycoerythrin from Porphyridium cruentum. Plant Physiol.44: 1629-1638. Gantt, E., 1981, Phycobilisomes. Ann. Rev. Plant. Physiol. 32: 327-347. Gantt, E. and C. A. Lipschultz, 1974, Phycobilisomes of Porphyridium cruentum: pigment analysis. Biochemistry 13: 2960-2966. Gantt, E. and C. A. Lipschultz, 1980, Structure and phycobiliprotein composition of phycobilisoines from Griffithsia pacifica (Rhodophyceae). J. Phycol. 16: 394-398. Geng, X. and F. E. Regnier, 1984, Retention model for proteins in reversed-phase liquid chromatography. J. Chromatogrr. 296: 15-30. Glazer, A. N., 1977, Structure and molecular organization of the photosynthetic accessory pigments of cyanobacteria and red algae. Mol. Cell. Biochem. 18:125-140. Glazer, A. N., 1982, Phycobilisomes: structure arid dynamics. Ann. Rev. Microbiol. 36: 173-198. Glazer, A. N., 1987, Phycobilisomes: assembly and attachment. In: Fay, P. and Van Baalen, C.(Eds.), The Cyanobacteria, Elsevier Science Publishers, Amsterdam, pp.69-94. Glazer, A. N., 1989, Light guides: Directional energy transfer in a photosynthetic antenna. J. Biol. Chem.264: 1-4. Glazer, A. N., G. Cohen-Bazire and R. Y. Stainier, 1971, Comparative immunology of algal biliproteins. Proc. Nat. Acad. Sci. USA 63: 3005-3008. Glazer, A. N. and C. S. Hixson, 1975, Characterization of R-phycocyanin. J. Biol. Chem. 250: 5487-5495. Glazer, A. N. and C. S. Hixson, 1977, Subunit structure and chromophore composition of Rhodophytan phycoerythrins. J. Biol. Chem. 252: 32-42. Glazer, A. N., J. A. West and C. Chan, 1982, Phycoerythrins as chemotaxonomic markers in red algae: a survey. Biochem. Sys. Ecol. 10: 203-215. Glazer, A. N., S. W. Yeh, S. P. Webb and J. H. Clark, 1985, Disk-to-disk transfer as the rate-limiting step for energy flow in phycobilisomes. Science 227: 419-423. Grossman, A. R., P. G. Lemaux, P. B. Conley, B. U. Bruns and L. K. Anderson, 1988, Characterization of phycobiliprotein and linker polypeptide genes in Fremyella diplosiphon and their regulated expression during complementary chromatic adaptation. Photosyn. Res. 17: 23-56. Henry, M. P. 1989, Ion-exchange chromatography of proteins and peptides, In: HPLC of Macromolecules: a Practical Approach, R. W. A. Oliver, Ed. IRL Press, New York, pp. 91-125. Hirose, H. and S. Kumano, 1966, Spectroscopic studies on phycoerythrins from rhodophycean algae with special reference to their phylogenetical relation. Bot. Mag. Tokyo 79: 105-113. Hirose, H., S. Kumano and K. Madono, 1969, Spectroscopic studies on phycoerythrins from Cyanophycean and Phodophycean algae with special reference to their phylogenetical relations. Bot. Mag. Tokyo 82: 197-203. Hoffmann, L., L. Talarico and A. Wilmotte, 1990, Presence of CU-phycoerythrin in the marine benthic blue-green alga Oscillatoria of corallinae. Phycologia 29(1): 19-26. Honsell, E., V. Kosovel and L. Talarico, 1984, Phycobiliprotein distribution in Rhodophyata: studies and interpretations on the basis of their absorption spectra. Bot. Mar. 27: 1-16. Johns, D. 1989, Columns for HPLC seperation of macromolecules. In: HPLC of Macromolecules: Practical Approach, R. W. A. Oliver, Ed. IRL Press, New York, pp. 1-42. Katzenstein, G. E., S. A. Vrona, R. J. Wechsler, B. L. Steadman, R. V. Lewis and C. R. Middaugh, 1986, Role of conformational changes in the elution of proteins from reversed-phase HPLC columns. Proc. Natl. Acad. Sci. USA 83: 4268-4272. Kikuchi, R., K. Ashida and S. Hirao, 1979, Phycobilins in different color types of Porphyra yezoensis Ueda. Nippon Suisan Gakkaishi 45(11): 1461-1464. Klotz, A. V. and A. N. Glazer, 1985, Characterization of the bilin attachment sites in R-phycoerythrin. 260:4856-4863. Koller, K.-P. and W. Wehrmeyer, 1975, B-phycoerythrin from Rhodella violacea characterization of two isoproteins. Arch. Microbiol. 104: 255-261. Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227: 680-685. Lagarian, J. C., A. V. Klotz, J. L. Dallas, A. Z. Glazer,J. E. Bishop, J. F. O’Connell and H. Rapoport, 1988, Exclusive A-ring linkage for singly attached phycocyanobilins and phycoerythrins in phycobiliproteins. J. Biol. Chem. 263: 12977-12985. Lee, B. D., 1978, Separation, identification and quantitative determination of photosynthetic pigments in seaweeds.Trans. Acad. Sci. USSR Far East Sci. Centre, Inst. Mar. Biol. 11:38-54. Lowry,O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall, 1951, J. Biol. Chem. 193: 265-275. Lu, X. M., K. Benedek and B. L. Karger, 1986, Conformational effects in the high-performance liquid chromatography of proteins. J. Chromatogr. 359: 19-29. Lundell, D. J. , A. N. Glazer, R. J. Delange and D. M. Brown, 1984, Billin attachment sites in α and β subunits of B-phycoerythrin. J. Biol. Chem. 259: 5472-5480. MacColl, R., J. J. Lee and D. S. Berns, 1971, Prote-02Xin aggregation in C-phycocyanin. Biochem. J. 122: 421-426. MacColl, R., K. Csatorday, D. S. Berns, E. Traeger, 1980, Chromophore interactions in allophycocyanin. Biochemistry 19:2817-2820. Mallery, C. H. and N. Richardson, 1971a, Disc gel electrophoresis of biliproteins. Plant and Cell Physiol.12: 997-1001. Mallery, C. H. and N. Richardson, 1971b, Disc gel electrophoresis of proteins of Rhodophyta. Proc. Int. Seaweed Symp. 7: 292-300. McLachlan, J., 1964, Some consideration of the growth of marine algae in artifical media. Can. J. Microbiol. 10:769-782. Mizuno, H., N. Iso, T. Saito, F. Ohzeki and T. Hatano, 1982, Identification of phycocyanin isolated from Porphyratenera and Porphyra yezoensis. Nippon Suisan Gakkaishi 48(7): 971-974. Morschel, E., W. Wehrmeyer and K-P Koller, 1980, Biliprotein assembly in the disc-shaped phycobilisomes of Rhodellaviolacea: Electron microscopical and biochemical analysis of B-phycoerythrin and B-phycoerythrin-C-phycocyanin aggregate. Eur. J. Cell Biol. 21: 319-327. Nagy, J.O., J. E. Bishop, A. V. Klotz, A. N. Glazer and H.Rapoport, 1985, Billin attachment sites in the α, β and γ subunits of R-phycoerythrin. J. Biol. Chem. 260:4864-4868. O’carra, P., 1965, Purification and N-terminal analyses of algal biliproteins. Biochem. J. 94: 171-174. Ogawa, H., H. Mizuno, T. Saito, Y. Yamada, T. Oohusa and N. Iso, 1991, Effects of pH on the conformation of phycoerythrin from nori Porphyra sp.. Nippon Suisan Gakkaishi 57(5): 899-903. O’hEocha, C., 1965, Biliproteins of algae. Ann. Rev. Plant Physiol. 16: 415-434. Ong, L. J. and A. N. Glazer, 1991, Phycoerythrins of marine unicellular cyanobacteria. J. Biol. Chem. 266: 9515- 9527. Redlinger, T. and E. Gantt, 1981, Phycobilisome structure of Porphyridium cruentum. Plant Physiol. 68: 1375-1379. Schoenleber, R. W., D. J. Lundell, A. N. Glazer and H. Rapoport, 1984, Bum attachment sites in a and B subunits of B-phycoerythrin. J. Biol. Chem. 258: 5481-5484. Sidler, W., B. Kumpf, W. Rudiger and H. Zuber, 1986, The complete amino-acid sequence of C-phycoerythrin from the cyanobacterium Fremyella diplosiphon. Biol. Chem. 367:627-642. Stadnichuk, I. N., D. I. Odintsova and A. I. Strongin, 1984, Molecular organization and chromophore composition of R-phycoerythrin from the alga Callithamnion rubosum. Mol. Biol. 18: 272-277. Swanson, R. V. and A. N. Glazer, 1990, Seperation of phycobiliprotein subunits by reverse-phase high-pressure liquid chromatography. Anal. Biochem. 188: 295-299. Talarico, L., 1990, R-phycoerythrin from Audouinella saviana (Nemaliales, Phodophyta): Ultrastructural and biochemical analysis of aggregates and subunits. Phycologia 29(3):292-302. Van der Velde, H. H., 1973a, The use of phycoerythrin absorption spectra in the classification of red algae. Acta Bot. Neerl. 22: 92-99. Van der Velde, H. H., 1973b, The natural occurrence in red algae of two phycoerythrins with different molecular weights and spectral properties. Biochem. Biophys. Acta.303: 246-257. Wedemayer, G. J., D. E. Wemmer and A. N. Glazer, 1991, Phycobilins of cryptophycean algae. J. Biol. Chem. 266:4731-4741. Yu, M-H., A. N. Glazer, K. G. Spencer and J. A. West, 1981, Phycoerythrins of the red alga Callithamnion. Plant Physiol. 68: 482-488. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75930 | - |
dc.description.abstract | 頭髮菜 Banqia atropurpurea ,長葉紫菜 Porphyra dentata , 狹葉紫菜P. anqusta ,蠕枝藻 Helminthocladia australis 與粉枝藻 Liaqora orientalis 等五種紅藻之絲狀體為其生活史中的一段時期藉由環境條件的控制可大量培養於實驗室中,由於其生長的穩定及藻膽色素的抽取容易,比葉狀體更適合色素的抽取與分析研究。 從頭髮菜B.atropurpurpurea的絲狀體,藻色素蛋白可以磷酸緩衝液萃取出,再經製備型膠濾層析反覆操作可純化出藻紅素( Phycoerythrin )。首先利用純質藻紅素建立了分離藻紅素異價體之高效能陰離子交換層析系統與分離藻紅素次單元體之高效能逆相層析系統。在高效能陰離子交換層析的表現,發現頭髮菜藻紅素實際乃三種以上異價蛋白所組成的,不同的藻紅蛋白異價體成份在高效能逆相層析系統下可看出各含有相同的α、β次單元體與不同的γ次單元體。 結合了製備型膠濾層析與高效能陰離子交換層析,成功地純化了頭髮菜之藻紅素、藻藍素與異藻藍素,並以此純質色素蛋白藉由蛋白質定量數據與色素在可見光譜下的吸光特性,求出各純質在波長 565 nm 、 618nm 、 650nm 處之消光係數,並藉之導出在粗萃取物中測定各色素含量的計算公式,各色素蛋白含量如下:(單位為 mg / ml ) PE = 0.21A 565-0.16A618 + 0.05A650 PC = 0.56A618-0.35A650 APC = 0.58A650-0.52A618 藉由光譜學的分析,發現頭髮菜藻紅素各異價體成份間的光譜性質並不完全相同,次單元體衍蛋白分子部分,會影響到相同發色團的光譜表現。藻紅素次單元體α含有發色團 PEB ,β與γ次單元體則含有兩不同的發色團 PUB 、PEB 但在相對量上並不相同。 更進一步利用純質色素在高效能陰離子交換層析的表現,成功地建立各藻色素的定性分析系統,同時亦發現各不同藻之藻紅素實際乃由許多不同的異價蛋白所組成。以高效能陰離子交換層析比較二科四屬共五種之紅藻藻膽蛋白組成,由各藻膽蛋白層析的表現中,發現P.anqusta ,P . dentate為紫菜屬的兩種不同植物,於相同的培養條件下,其絲狀體藻膽蛋白的組成幾乎一致,與同為頭髮菜科的頭髮菜B.atropurpurea之藻膽蛋白組成相近,而與蠕枝藻科的L.orientalis,H.australis 兩種絲狀體有較大的差異。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:32Z (GMT). No. of bitstreams: 0 Previous issue date: 1992 | en |
dc.description.tableofcontents | I 、前言. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 II、材料與方法 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 甲、藻膽蛋白之高效能層析分析 . . . . . . . . . . . . . . . . . . . . . 7 乙、頭髮菜絲狀體藻膽蛋白的研究 . . . . . . . . . . . . . . . . . . . 8 丙、不同藻種絲狀體之藻膽蛋白在高效液相層析的表現. . . . . . . 11 III、結果 . . . . . . . . . . . . . . . . . . . . . . . . 13 甲、. . . . . . . . . . . . 13 乙、 . . . . . . . . . . . . . . . . . . . 16 丙、 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 IV 、討論 . . . . . . . . . . . . . . . . . . . . . 22 甲、. . . . . . . . . . . . . . . . . . . . 22 乙、. . . . . . . . . . . . . . . . . . . . . . . . 26 丙、. . . . . . . . . . . . . . . . . . . . . . 30 參考文獻. . . . . . . . . . . . . . . . . . . . . . . . . . 32 附表. . . . . . . . . . . . . . . . . . . . . . . . . 40 附圖. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 附錄.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 | |
dc.language.iso | zh-TW | |
dc.title | 紅藻絲狀體藻膽蛋白之高效層析及光譜之研究 | zh_TW |
dc.title | Chromatographic analysis and identification of phycobiliproteins from filamentous phase of some red algae | en |
dc.date.schoolyear | 80-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 81 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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