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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳彥州 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:16Z | - |
| dc.date.available | 2021-07-01T08:16:16Z | - |
| dc.date.issued | 1992 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75898 | - |
| dc.description.abstract | 文蛤水產呼腸孤病毒（CAV）為本研究室於民國七十五年自文蛤體內所分離出來。本論文分為二部份：（一）研究病毒複製及轉錄的過程，以瞭解病毒的生活史，期能發展出有效的防治方法。利用「32P」-Orthophosphate標定病毒RNA，再以3％Agarose-urea gel分析，發現在感染後18小時才有RNA的合成，且合成量隨時間增長而漸增；再用S1-Nuclease處理不同時間標定的RNA，以10％SDS-PAGE分析發現，在感染後18小時即有dsRNA出現。此外，自培養液回收病毒，發現在感染後36小時才有標定之病毒釋出。由以上可知CAV自感染細胞後12-18小時之間開始轉錄及複製，而在感染後36小時才開始將病毒顆粒釋出，所以一個病毒複製循環需時約36小時；在感染後12小時之前均無標定之病毒RNA出現，故推測CAV感染細胞後應有一段大約為12小時的潛伏期，目標是由病毒total RNA的放射量與dsRNA的比較，推測應先轉錄後複製。（二）探討CAV之Guanylyltransferase的特性。CAV的病毒結構蛋白λ2，可與GTP形成GMP-λ2複合體，故推測λ2具有Guanylyltransferase的功能。GMP-λ2複合體的形成受時間，溫度，病毒蛋白量，二價離子濃度等因素影響，且受焦磷酸（Pyrophosphate），DTT（dithiothreitol），Sodium Phosphate等分子抑制。若加入活體外轉錄之RNA，或RNA Ladder，均會使GMP-λ2複合體消失，且GMP被轉移至RNA上，故確定λ2具有加帽酵素之Guanylyltransferase的功能。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1992 | en |
| dc.description.tableofcontents | 摘 要……………………………………1 壹、前 言……………………………………2 貳、文獻整理……………………………………3 一、水產呼腸孤病毒（Aquareovirus）的發現……………………………………5 （一）GSV……………………………………5 （二）13P2……………………………………5 （三）CSV……………………………………6 （四）CRV……………………………………6 （五）其他……………………………………7 二、水產呼腸孤病毒（Aquareovirus）的分類地位……………………………………7 三、Reovirus……………………………………9 （一）Morphology……………………………………9 （二）基因組成……………………………………10 （三）蛋白質組成……………………………………10 （四）病毒蛋白醇素活性……………………………………11 （五）Reovirus的生活史……………………………………12 四、Rotavirus……………………………………13 （一）Morphalogy……………………………………14 （二）基因組成及產物……………………………………15 （三）Rotavirus的生活史……………………………………17 參、實驗材料及方法……………………………………20 （一）實驗材料……………………………………20 （二）實驗方法……………………………………21 肆、實驗結果……………………………………32 一、GTP-酵素複合體的位置……………………………………32 二、溫度對[α-32P]GTP結合反應的影響……………………………………32 三、鎂離子對GMP－λ2複合體形成的影響……………………………………33 四、GTP的影響對GMP－λ2複合體形成的影響……………………………………33 五、抑制物對複合體的影響……………………………………33 六、結合反應時間對GMP－λ2複合體的影響……………………………………34 七、病毒蛋白量對GMP-λ2複合體結合的影響……………………………………34 八、λ2對GTP結合的專一性……………………………………34 九、RNA對複合體結合的影響……………………………………35 十、確定CAV病毒的外鞘蛋白質組成……………………………………35 十一、EDTA對病毒顆粒的影響……………………………………36 十二、CAV轉錄及複裂的過程……………………………………36 伍、討 論……………………………………37 陸、參考資料……………………………………40 附 圖……………………………………f1 | |
| dc.language.iso | zh-TW | |
| dc.title | 文蛤病毒轉錄及複製過程及加帽酵素之研究 | zh_TW |
| dc.title | The Studies on Transcription and Replication Processes and Guanylyltransferase Function of Clam Aquareovirus (CAV) | en |
| dc.date.schoolyear | 80-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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