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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 吳素幸 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:10Z | - |
| dc.date.available | 2021-07-01T08:16:10Z | - |
| dc.date.issued | 1992 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75884 | - |
| dc.description.abstract | 植物在高溫逆境下會合成大量的低分子量熱休克蛋白質(LMW HSPs)，在大豆中，此LMW HSPs 70-100％硫酸銨集濃分劃區內的蛋白質具有熱保護能力，可以使植物可溶性蛋白質在高溫下的變性量降低達50％，此LMW HSPs所保護的對象可能是與膜系統相關的蛋白質，而且沒有任何一種蛋白質受保護的情形較為明顯，不同離子強度對此熱保護作用並無顯著影響。為更進一步證實此熱保護能力來自LMW HSPs，而不是其他HSPs所造成的影響，故直接以純化的水稻LMW HSP融合蛋白質(fusion protein)進行熱保護實驗，結果證實LMW HSP的確能提供熱保護效應，而且熱保護能力會隨著所加入水稻LMW HSP融合蛋白質的增加而增加。除此之外，此熱保護能力在不同植物種類之間具有共通性；另一方面，也對LMW HSPs累積的量與熱保護能力之間的相關性作一探討。 | zh_TW |
| dc.description.abstract | Plants can produce a large amount of Low Molecular Weight heat shock proteins under heat stress. The LMW HSPs enriched 70-100% ammonium-sulphate fraction has the thermoprotection ability to prevent the soluble protein from denaturation under heat stress, and the protection efficiency can reach 50%. The LMW HSPs have the tendency to protect the membrane-related proteins but have no substrate-specificity. Different salt concentration has little effect on this thermoprotection ability. For further confirmation of the thermoprotection ability is mainly from the LMW HSPs, I used the purified rice LMW HSP fusion protein as LMW HSP donator and varified that the LMW HSP solely can contribute the thermoprotection ability to plants. I also found the protection efficiency is proportional to the amount of LMW HSPs added. Besides these, the thermoprotection ability is changeable among different kind of plants. On the other hand, I have tried to find out the relationship between the amount of LMW HSPs accumulation and the thermoprotection ability. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:10Z (GMT). No. of bitstreams: 0 Previous issue date: 1992 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………1 英文摘要……………………………………………………2 前言……………………………………………………3 材料與方法……………………………………………………15 結果……………………………………………………27 討論……………………………………………………48 參考文獻……………………………………………………54 | |
| dc.language.iso | zh-TW | |
| dc.title | 植物低分子量熱休克蛋白質的生理功能 | zh_TW |
| dc.title | The Biological Function of Low Molecular Weight Heat Shock Proteins in Plants | en |
| dc.date.schoolyear | 80-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 61 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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