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  1. NTU Theses and Dissertations Repository
  2. 生命科學院
  3. 動物學研究所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75857
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dc.contributor.author李德章zh_TW
dc.date.accessioned2021-07-01T08:15:56Z-
dc.date.available2021-07-01T08:15:56Z-
dc.date.issued1976
dc.identifier.citation1. Allfrey, V. G. 1974. Iaolation of nuclei from the thymus. Methods in Enzymol. 31:246.
2. Allfrey, V. G., V. C. Littau & A. E. Mirsky. 1964. On the role of histones in regulating ribonucleic acid synthesis in the cell nucleus. Proc. Nat. Acad. Sci. USA 49:414.
3. Anderson, K. M., H. Chance & N. Kadohama. 1975. Separation of transcript-tionally active from less active rat ventral prostate chromatin. Exp. Cell Hes. 94:176.
4. Ansein, A. T., L. S. Hnilica, T. C. Spesberg & S. L. Kehm. 1971. Structure studies on chromatin and nucloproteins. Thermal denaturation profiles recorded in the presence of ura. Biochemistry 10:4793.
5. Arrighi, E. E., T. C. Hsu, P. Saunders □ G. F. Saunders. 1971. Localization of repetitive DNA in chromosomes of Microtus agrestis by means of in situ hybridization. Chiiomosoma 32:24.
6. Baldwin, J. P., P. G. Boseley, E. M. Bradbury & K. Ibel. 1975. The subunit structure of eukaryotic chromosome. Nature 253:245.
7. Bekhor, I.G. M. Kung & J. Bonner. 1969. Sequence specific interaction of DNA and chrmosomal protein. J. Mol, Biol. 39:351.
8. Berkowitz, E. M. & P. Doty. 1975. Chemical and physical properties of fractionated chromatin. Proc. Nat. Acad. Sci. USA 72:3328.
9. Bernardl, G. 1969. Chromatography of nucleic acids on hydroxyapatite. I I. Chromatography of denature DNA. Biochim. Biophys. Acta 174:435.
10. Bernardi, G. 1971. Chromatography of nucleic acids on hydroxyapatite column. Methods in Enzymol. 22 (D):95.
11. Bhorjee, J. S. & T. Pederson. 1973. Chromatin: Its isolation from cultured mammalian cells with particular reference to contamination by nuclear ribonucleoprotein particles. Biochemistry 12:2766.
12. Billing, R. J. & J. Boriner. 1972, The structure of chromatin as revealed by deoxyribonuclease digestion studies. Biochim. Biophys. Acta 281:453.
13. Bonner, J., G. R. Chalkley, M. Daharus, 0. Fambrogh, F. Fujimu□, R. C. Huang, J. Huberman, R. Jensen, K. Marushige, 11. Ohlenbrusach, B. Oliver & J. Widhoim. 1968. Isolation and characterization of chromosomal nucleoproteins. Methods in Enzymol. 12 (B):3.
14. Bradbury, E. M., H. V. MoLgaare, R. M. Stephens, E. W. Johns & L. A. Bolun. 1972. X-ray studies of nucleoproteins depleted of lysine-rich histone. Eur. J. Biochem. 31:474.
15. Brown, S. W. 1966. Heterochromatin. Science 151:417.
16. Burkholder, G. D. & M. G. Weaver. 197. Differential accessibility of DNA in extended and condensed chromatin to pancreatic DNase I. Exp. Cell Res. 92:518.
17. Bustin, M. & B. D. Stollar. 1972. Imminochemical specificity in lysine-rich histone subfractions. J. Biol. Chrm. 247:5716.
18. Byroet, F. 1966. Metabolic integrity of deoxyribonucleohistones, J. Mol. Biol. 17:311.
19. Chan, C-B. 1975. Reconstltution of cbromatin: Mode of reassociation of chromosomal proteins. Biochemistry 14:900.
20. Chauveau, J., Y. Moule' & C. H. Rou11er. 1956. Isolation of pure and unaltered liver nuclei morphology and biochemical components. Exp. Cell Res. 11:317.
21. Chevalliver, M. R. & G. Bernardi. 1968. Residual transforming activity of denatured Haemophilus Lnfluenzae DNA. J. Mol, Biol. 32:437.
22. Comings, D. E. 1970. Quantitative autoradiography of heterochromatin replication in Microtus agrestis. Chromosoma 29:423.
23. Comings, D. E. 1974. The structure of human chromosome, in The Cell Nucleus (H. Busch, ed.) 1:538.
24. Comings, D. E. & D. C. Harris 1975, Nuclear proteins. I. Electrophoretic comparison of mouse nucleoli, heterochromatin, euchromatin and contractile proteins. Exp. Cell Rae. 76:161.
25. DeLange, R. J., D. M. Fambrough, E. L. Smith & J. Bonner. 1969. Calf and pea histone IV. II. The complete amino acid sequence of calf thymus histones IV, presence of E-N-acetyllysine. J. Biol. Chem. 144:319.
26. DeLange, R. J. & E. L. Smith. 1971. Histones: structure and function, Ann, Rev. Biochem. 40:279.
27. Eckhardt, R. A. & J. G. Gall. 1971. Satellite DNA associated with hetero-chroiatin in Rynchociara. Chromosoina 32:407.
28. Elgin, S. C. a. & J. Bonner. 1972. Partial fractionation and chemical characterization of the major nonhione chromosomal proteins. Biochemistry 11:772.
29. Elgin, S. C. R. & H. Weintraub. 1975. Chromosomal proteins and chromatin atructure. Ann. Rev. Biochem. 44:725.
30. Flamm, W. G. 1972. Highly repetitive sequence of DNA in chromosomes. Internat. Rev. Cytol. 32:1.
31. Frenster, J. H. 1971. Electron microscopic localization of acridine orange binding to DNA within human leukemic bone. Cancer R□s. 31:1128.
32. Frenster, J. H., V. G. Allfrey & A. E. Mirsky. 1963. Repressed and active chromatin isolated from interphase lymphocytes. Proc. Nat. Acad. Sci. USA 50:1026.
33. Gilmour, R. S. & J. Paul. 1969. RNA transcribed from reconstituted nucleoprotein is similar to nature RNA. J. Mol. Biol. 40:137.
34. Gilrnour, R. S. & J. Paul. 1973. Tissue-specific transcription of globin gene in isolated chromatin. Proc. Nat. Aced. Sci. USA 70:3440.
35. Gosden, J. R. & A. R. Mitchell. 1975. Characterisation of DNA from corensed and dispersed human chromatin. Exp. Cell Roe. 92:131.
36. Gotterfeld, J. M., W. T. Garrard, C. Bagi, ft. F. Wilson & J. Bonnor. 1974. Partial purification of the template-active fraction of chromatin: A preliminary report Proc Nat. Acad. Sci. USA 71:2193.
37. Gotterfeld, J. M., ft. F. Murphy & J, Bonner. 1975. Structure of transcriptionally active chromatin, Proc. Nat. Aced. Sci. USA 72:4404.
38. Harwood, R. 1974. Cell separation by gradient centrifugation. Internat. Rev, Cytol. 38:369.
39. Reitz, E. 1928. Das Haterochromatin der Moose I. Jaheb. Weiss. Bat. 69:762.
40. Hemminki, K. 1975. Synthesis of chromatin proteins in resting and stimulated human lymphocyte populations. Exp. Cell Roe. 93:63.
41. Hensen, P. & I. O. Walker. 1971 The structure of nucleohistones: Hydrodynamic behavior at high ionic strength. Eur. J. Biochem. 22:1.
42. Hsu, T. C. 1962. Differential rate in RNA synbhesis between euchromatin and heterochromatin. Exp. Cell Rea, 27:332.
43. Hsu, T. C. 1973. Longitudinal differentiation of chromosomes. Ann. Rev. Qenet. 7:153.
44. Huang, R. C. & J. Bonner. 1962. Histone: A repressor of chromosomal RNA synthesis. Proc. Nat. Aced. Sci. USA 48:1216.
45. Huang, R. C. C. & P. C. Huang. 1969. Effect of protein-bound RNA associated with chick embryo chromatin on template specificity of the chromatin. J. Mol. Biol. 39:365.
46. Humason, G. C. 1967. Animal Tissue Technique (2nd ed.).
47. Itzhaki, R. F. 1971. The arrangment of proteins on the deoxyribonucleic acid in chromatin. Biochem. J. 125:221.
48. Janowski, M., D. S. Nasser & B. J. McCarthy. 1972. Fractionation of mammalian chromatin. Gene. transcription in reproduction tissue. Karolinska Symp. 5:112.
49. Johson, J. D., A. S. Douvas & J. Bonner. 1974. Chromosomal proteins. Internat. Rev. Cytol. 4 (supple.):273.
50. Jones, K. W. 1970. Chromoaomal and nuclear location of mouse satellite DNA in individual cell. Nature 225:912.
51. Kinkade, J. N. 1969. Qualeitative specific differences and quantitative differences in distribution of lysine-rich histories. J. Biol. Chem. 244:3375.
52. Kleinsmith, L. J., J. Heidema & A, Carroll. 1970. Specific binding of rat liver nuclear proteins to DNA. Nature 226:1025.
53. Kornberg, it. D. 1974. Chromatin structure: A repeating unit of histones and DNA. Science 184:868.
54. Kornberg, R. D. & J. D. Thomas. 1974, Chromatin structure: Oligomers of the histones. Science 184:865.
55. Kostraba, N. C., R. A. Montagna & T. Y. Wang. 1974. Study of the loosely bound nonhistone chromatin proteins. J. Biol. Chem. 250:1548.
56. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 227:680.
57. Langan, T. A., S. C. Rall & R. D. Cole, 1971. Variation in primary structure at a phosphorylation site in lysine-rich histories. J. Bi□l. Chem. 246:1942.
58. Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265.
59. MacGtillivary, A. J. & D. Richwood. 1974. The heterogeneity of mouse chromatin nonhistone proteins as evidenced by two-dimensional polyacryl-amide gel electrophoresis and ion-exchange chromatography. Eur. J. Biochem. 41:181.
60. Magee, B. B., J. Paolettl & P. T. Magee. 1975. Separation of lymphocyte chromatin into template-active fraction with apecificity for eukaryotic RNA polymerase II or prokaryotic RNA polymerase. Proc. Nat. Acad. Sci. USA 72:4830.
61. McCarthy, B. J., J. T. Nishiura, D. Doinecke, D. S. Nasser & C. B. Johnson. 1973. Transcription and chromatin structure. CSHSQB 38:763.
62. McConaugh, B. L. & B. J. MacCarthy. 1972. Fractionation of chromatin by thermal chromatography. Biochemiatry 11:998.
63. McGhee, J. D. & C. B. Kimnel. 1975. Evldence for a subunit structure of chromatin in mouse myeloma cells. Chrmoaoma 52:189.
64. Mirsky, A.E., C. J. Burd,.ck, E. H.. Davidson & V. C. Littau. 1968. The role of lysine-rich histone in the maintenance of chromatin structure in metaphase chromosome. Proc. Nat. Acad. Sci. USA 61:592.
65. Murphy, K. C. Jr., S. H. Ball, J. H.. Shepherd & r. S. Weise. 1973. Fractionation of mouse myeloma chromation. Biochemistry 12:3843.
66. Murray, K., E. K. Bradbury, C. Crane-Robison, R. M. Stephens, A. J. Haydon & A, R. Peacockes. 1970. The dissociation of chicken erybhrocyte deoxyribonucleoprotein and some properties of its partial nucleoproteina. Biochem. J. 120:859.
67. Nicolini, C & R. Baserga. 1975. Role of nonhistone chromosomal proteins in determining circular spectra of chromatin. Arch. Biochem. Biophys. 169:678.
68. Olina, A. L., R. D. Carlson & D E. Olins. 1975. Visualization of chrowatin substructure: v bodies, J. Cell Biol. 64:528.
69. Olins, A. L. & B. E. Olins. 1974. Spheroid chromatin units, Science 183:330.
70. Qlson, M. O. J. & II. Busch. 1974. Nuclear proteins, in The cell Nucleus (H. Busch, ed.) 3:211.
71. O'Malley, B. W. & A. R. Moans.1974. Effects of female steroid hormones on target cell nuclei, in The Cell Nucleus (H. Busch, d.) 3:379.
72. Oostarhof, D. K., J. C. Hozier & R. L. Hill. 1975. Nuclease action on chromatin: Evidence for discrete, repeated nucleoproteins units along chromatin fibrils. Proc. Nat. Acad. Sci. USA 72:633.
73. Panyim, S., D. Bilek & R. Chalkley. 1971. An electrophoresis comparison of vertebrate histonas. J. Biol, Chem. 246:4206.
74. Panyim, S. & R. Chalkley. 1969. The h3terogeneity of histones. I. A quantitative analysis of Calf thymus in very long polyacrylamid gels. Biochemistry 8:3972.
75. Panyim, S. & R. Chalkley. 1969. High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 130:337.
76. Pardue, M. L. & J. C. Gall. 1970. Chromosomal localization of mouse satellite DNA. Science 168:1356.
77. Pardon, J. F. & M. H. F. Wilkins, 1972. A model for supercoil nucleo-histones. J. Mol. Biol. 68:115.
78. Paul, J. & R. S. Gilmour 1968 Organ-specific restriction of transcription in mammalian chromavin. J. MoL Biol. 34:305.
79. Rattner, J. B., A. Branch & B. A. Hamkalo. 1975. Electron microscopy of whole mount metaphase chaomosome. Chromosoma 52:329.
80. Reeck, G. R., R. T. Simpson & H. A. Sober. 1972. Resolution of a spectrum of nucleoprotein species in sonicated chroinatin. Proc. Nat. Acad. Sci. USA 69:2317.
81. Rill, R. & H. £. van Holde, 1973. Properties of nuclease resistant fragment of calf thymus chromatin J. Biol. Chem. 248:1080.
82. Roark, D. E., T. E. Geoghegan &. H. Keller. 1714. A two-subunit histone complex from calf thymus, Biochem. Biophys. Res. Commun. 59:304.
83. Romer, A. S. 1970. The Vertebrate body (4th ed.) pp.347.
84. Saita, H. & K. Miur. 1963. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619.
85. Schnedle, W. 1974. Banding patterns in chromosomes. Internat. Rev. Cytol. 4 (supple.):237.
86. Senior, N. B., A. L. Olins & D. E. Olins. 1975. Chromatin fragments resembling v bodies. Science 187:173.
87. Shaw, B. R., J. L. Gorden, L. G. Sahasrabuddhe & K. E. van Holde. 1974. Chromatographic separation of chromatin subunits. Biochem. Biophys. Res. Commun. 61:1193.
88. Shea, M. & L. J. Kieinsmith. 1973. Template-specific stimulation of RNA synthesis by phosphorylated nonhistone chromatin proteins. Biochem. Biophys. Res. Commun. 50:473.
90. Shelton, K. & T. Hamilton. 1969. Role of chromatin in estrogen action in the uterus. II. Hormone-induced synthesis of nonhistone acidic proteins which restore histone-inhibted DNA-dependent RNA synthesis. Proc. Nat. Aced. Sci. USA 63:465.
89. Shelton, K. & V. G. Allfrey. 1970. Selective synthesis of a nuclear acidic protein in liver cells stimulated by cortisol. Nature 228:132.
91. Simpson, R. T. 1972. Modification of caromatin by trypsi. The role of proteins in maintenance of deoxyribonaleic acid. Biochemistry 11:2003.
92. Simpson, R. T. 1973. Separation of transcriable and repressed chroamtin. Current Topics in Biochenistry 3:135.
93. Simpson, B, T. 1974. Distribution of RNA polymerase binding sites in fractionated chromatin. Froc. Nat. Acnd, Sci. USA 71:2740.
94. Simpson, R. T. 1974. The distribution of histones and nonhistone proteins in the ECTHAM-cellulose fractions of chromatin from several tissues. Eur. J. Biochem. 49:407.
95. Stein, G. S. & R. Baserga. 1970. Continued synthesis of nonhistone chromosomal proteins during mitosis Biochem. Biophys. Res. Commun. 41:715.
96. Stein, G. H. & T. W. Broun. 1972. The synthesis of acidic chromosomal. protsins during cell cycle of HeLa S-3-cell. J. Cell Biol. 52:292.
97. Tashiro, T. & M, Kurolawl. 1975. A conformation of chromatin. Eur. J. Biochem. 60:569.
98. Tata, J. B. 1974. Isolation of nuclei from liver and other tissue. Methods in Enzymol. 31:252.
99. Teng. C. S., C. T. Teng & V G. Allfrey. 1971 Studies of nuclear acidic proteins. J, Biol. Chem, 246:3597.
100. Wang, T. Y. 1972. Tissue specificity of nonhistone chromosomal proteins. Exp. Cell Res. 69:217.
101. Wang, T. Y. & L. M. Nyberg. 1974. Androgen receptors in the nonhistone protein fraction, of prostatic chromatin. Internat. Rev. Cytol. 39:1.
102. Webb. J. M. & H. B. Levy. 1958. New developments in the chemical deter-mination of nucleic acid. Method of Biochem. Analysis 6:10.
103. Weber, K. & H. Osborn. 1969. SDS-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406.
104. Woodcock, C. L. F., J. P. Safer & J. E. Stanchlield. 1976. Structure repeating units in chromatin. I. Evidence for their general occurence. E. Cell Res. 97:101.
105. Yasmineh, W. G. & J. J. Yunis 1969. Satellite DNA in mouse autosomal heterochromatin. Biochem, Biophye. Res. Commun. 35:779.
106. Yasmineh, W. 0. & J. J. Yunis. 1970. Localization of mouse satellite in conatitutive heterochromatin. Exp. Coll Res. 59:69.
107. Yasminah, W. G. & J. J. Yunis. 1971. Repetitive DNA of Microtus agreetis. Biochem. Biophys. Res. Coinmun. 43:580.
108. Yasminah, W. G. & J. J. Yunis. 1971. Satellite DNA in calt heterochromatin. Exp. Cell Res. 64:41.
109. Yasmineh, W. G. & J. J. Yunls. 1974. Isolation of mammalian heterochromatin and euchromatin. Methods in. Cell Biol. 3:151.
110. Yeoman, L. C., G. W. Taylor1 J. J. Jordan & H. Bush. 1973. Two-dimensional polyacrylamide gel electroporesis of chromatin proteins of normal rat liver and Nowikoff hepatoma ascites cells. Biochem. Biophys. Res. Commun. 53:1067.
111. Yunis, J. J., L.. Roldan, W. G. Yasmineh & J. C. Lee. 1971. Staining of satellite DNA in metaphase chromosomes Nature 231:532.
112. Yunis, J. J. & U. G. Yasmineh. 1970. Satellite DNA in constitutive hetero-chromatin of guinea pig. Sclence 168:263.
113. Yunis, J. J. & W. G. Yasmineh. 1971. Heterochromatin, satellite DNA, and cell function. Science 174:1200.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75857-
dc.description.abstract用不同的維心力可將豬肝及示成熟睪丸之染色質劃分成三部分。染色質F1之非酸性核蛋白及酸性核蛋白□DNA之比值均大於染色質F3。各種染色質之非負性核蛋白與DNA之比值□化較明顯。
將各種染色質抽取之DNA用□□□□色層分析發現DNA之組成並不相同,可能與重複DNA之含□有關。酸性核蛋白之電冰分析,發現肝□□成熟睪丸之於及DNA少許的差別,但是同組織各種染色體之酸性核蛋白無明顯的區別。
染色體貼F3與F1之紫外吸光體在260-230nm間有明顯的差別,在此範圍內染色質F3之吸光度低於F1,此可能與蛋白質之含量有關。若將染色質以3X NaCl-5Murca處理,則染色質F3與F1之紫外吸光譜之差異更明顯,因此能□□者之結□有所不同,染色質F1之結構可能較緊密。肝□之染色質F1及F3在260-230nm間,其吸光度均高與睾丸,此可能與組織內質之含量有關。
本實驗之初步結論?染色制裁學與F3之差異,主要與DNA及非酸性核蛋白之質與□□;而酸性核蛋白雌然□□上□有少許的變化,但質上卻無明顯的差別。
zh_TW
dc.description.provenanceMade available in DSpace on 2021-07-01T08:15:56Z (GMT). No. of bitstreams: 0
Previous issue date: 1976
en
dc.description.tableofcontents一、緒論 l
二、材料 5
三、方法 6
(一)組織切片 6
(二)純化細胞核 6
(三)染色質之劃分 6
(四)染色質化學組成之分析 7
(五)DNA之粹取及分析 10
(六)酸性核蛋白之電冰分析 12
(七)染色質紫外吸光譜之測定 14
四、結果 15
(一)純化細胞核 15
(二)染色質之化學組成 16
(三)DNA 之分析 17
(四)酸性核蛋白之電冰分析 19
(五)染色質紫外吸光譜 27
五、討論 27
六、中文摘要 36
七、參考文獻 37
dc.language.isozh-TW
dc.title染色質之比較研究zh_TW
dc.date.schoolyear64-2
dc.description.degree碩士
dc.relation.page44
dc.rights.note未授權
dc.contributor.author-dept生命科學院zh_TW
dc.contributor.author-dept動物學研究所zh_TW
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