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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | 郭玫鈴 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:50Z | - |
| dc.date.available | 2021-07-01T08:15:50Z | - |
| dc.date.issued | 1991 | |
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(1985) Papillomavirus infection of the lower genital tract: detection of viral DNA in gynecological swabs. Int. J. Cancer 35, 443-448. 6. Durst, M., Gissmann, L., Ikenberg, H. and zur Hausen, H. (1983) A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc. Natl. Acad. Sci. USA 80, 3812-3815. 7. Ikenberg, H., Gissmann, L., Gross, G., Grussendorf-Conen, E. I, and zur Harsen, H. (1983) Human papillomavirus type-16-related DNA in genital Bowen’s disease and in bowenoid papulosis. Int. J. Cancer 32, 563-565. 8. Syrjanen, S, M., Happonenn, R.P., Virolainen, E., Siivonen, L, and Syrjanen, K. J. (1987) Detection of human papillomavirus (HPV) structural antigens and DNA types in inverted papillomas and squamous cell carcinomas of the nasal cavities and paranasal sinuses. Acta Otolaryngol, 104, 334-341. 9. Syrignen, S.M., Syrianen, K. J. and Lamberg, M. A. 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(1989) Continued expression of HPV-16 E7 protein is required for maintenance of the transformed phenotype of cells cotransformed by HPV-16 plus EJ-ras. EMBO J. 8, 513-519. 36. Phelps, W. C., Yee, C, L., Hunger, K. and Howley, P. M.(1988) The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A. Cell 53, 539-547. 37. Yutsudo, M., Okamoto, Y. and Hakura, A. (1988) Functional dissociation of transforming genes of human papillomavirus type 16. Virology 166, 594-597. 38. Kanada, T., Furuno, A. and Yoshiike. K, (1988) Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells. J, Virol. 62, 610-613. 39. Tanaka, A., Noda, T., Tajima, H., Hatanaka, M. and Yoshiakiito (1989) Identification of a transforming gene of human papillomavirus type 16. J. Virol. 63. 1455-1469. 40. Halbert, C. L., Demers, G. W. and Galloway, D. A. (1991) The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65, 473-478. 41. Sato, H., Gruuno, A. and Yoshike, K. (1989) Expression of human papillomavirus type 16 E7 gene induces DNA synthesis of rat 3Y1 cells. Virology 168, 195-199. 42. Banks, L. Barnett, S. C. and Crook, T. (1990) HPV-16 E7 functions at the GI to S phase transition in the cell cycle. Oncogene 5, 833-839. 43. Werness, B. A., Levine, A.J. and Howley, P. M. (1990) Association of human papillomavirus type 16 and 18 E6 proteins with p53. Science 248, 76-79. 43. Werness, B.A., Levine, A.J. and Howley, P.M, (1990) Association of human papillomavirus type 16 and 18 E6 proteins with p53. Science 248, 76-79. 44. Dyson, N., Howleyk, P. M., Hunger, K. and Harlow, E. (1989) The human papillomavirus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243, 934-937. 45. Debiecrychter M., zukoskik. , Wang C.Y. and Wen W.W. (1991) Chromosomal characterizations of human nasal and nasopharyngeal cells immortalized by human papillomavirus type 16 DNA. Cancer Genetics and Cytogenetics 51, 51-62. 46. Sambrook, J., Fritsch, E. and Maniatis, T. (1989) Molecular cloning, a laboratory manual, second edition. Cold Spring Harbor Laboratory Press. 47. Cornelissen, M. T. E., Smits, H.L. Briet, M.A., van der Noordaa, J. and ter Schegget, J. (1990) Uniformity of the splicing pattern of the E6/E7 transcripts in human papillomavirus type 16 transformed human fibroblasts, human cervical premalignant lesions and carcinomas. J, Gen. Virol. 71, 1243-1246. 48. Smotkin, D, Prlokoph, H. and Wettstein, F. O. (1989) Oncogenic and nonocogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms, J. Virol. 63, 1441-1447. 49. Seedorf, K., Krammer, G, Durst, M. Suhai, S. and Rowekamp, W. G. (1985) Human papillomavirus type 16 DNA sequence. Virology 145, 181-185. 50. Cripe, T.P., Haugen, T.H., Turk, J.P., Tabatabai, F, Schmid, III, P.T. (1987) Transcriptional regulation of the human papillomavirus-16 E6-E7 promoter by a keratinocyte-dependent enhancer, and by viral E2 trans-activator and repressor gene products: implications for cervical carcinogenesis. EMBO J. 6, 3745-3753. 51. Gloss, B., Bernard, H.U., Seedorf, K. and Klock, G. (1987) The upstream regulatory region of the human papillomavirus-16 contains an E2 protein-independent enhancer which is specific for cervical carcinoma cells and regulated by glucocorticoid hormones. EMBO J. 6, 3735-3743. 52. Kozak, M. (1987) Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol. Cell. Biol. 7, 3438-3445. 53. Smotkin, D. and Wettstein, F. O. (1987) The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein. J. Virol. 61, 1686-1689. 54. Barbosa, M.S., Edmonds, C., Fisher, C., Schiller, J. T- , Lowy, D.R. and Vousden, K.H. (1990) The region of the HPV E7 oncoprotein homologous to adenovirus Ela and SV40 large T antigen contains separate domains for Rb binding and casein kinase II phosphorylation. EMBO J. 9, 153-160. 55, Munger, K., Bruce, W.A., Dyson, N., Phelps, W.C. , Harlow, E. and Howley, P. H. (1989) Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J.8, 409 9-4015. 56. Gage, J., Meyers, C. and Wettstein, F.O, (1990) The E7 proteins of the nononcogenic human papillomavirus type 6b (HPV-6b) and of the oncogenic HPV-16 differ in retinoblastoma protein binding and other properties. J. Virol. 64, 723-730. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75844 | - |
| dc.description.abstract | 為探討人類乳突狀第十六型病毒(Human Papillooma-virus Type16, HPV-16)的致癌特性，我們將完整的HPV-16 DNA輿質體(plasmid) pdMMT之DNA重組，並將重組後的DNA轉入正常人類鼻腔上皮細胞，結果這細胞成功地被轉型為不死的細胞株，命名為NW-1。由生長需求實驗中顯示NW-1細胞不死的現像是牛腦下垂體萃取(Bovine Pituitary Extract, BPE)表皮生長因數(Epidermal Growth Factor，EGF)或胰島素(Insulin，Ins)與HPV-16 DNA相互作用的結果。 當細胞培養在生長較佳的狀況下，Northern Hybridization的實驗結果發現細胞有較多的HPV-16所有基因轉錄?物。當細胞以BPE,EGF和Ins或EGF及Ins 處理20小時後，RNA slot blot Hybridization的實驗結果證明HVP-16所有基因的表現也會增加。HPV-16之E6及E7基因的表現利用RNA Polymerase Chain Reaction (RNA-PCR)偵測，結果發現細胞培養在不同的狀況下均有三種放大產物出現，其大小各?791,608及491Base Pairs，這些RNA-PCR產物量之多寡輿細胞生長之快慢或DNA合成並沒有直接的正相關。 | zh_TW |
| dc.description.abstract | To determine the oncogenic properties of Human Papillomavirus Type 16(HPV-16), we have been successful in transfecting normal human nasal epithelial cells with recombinant DNA containing complete HPV—16 genomic DNA. The results of growth requirement indicate that the immortalization of NW—1 cells likely is due to the interaction between the growth factors and HPV-16 DNA . Greater amount of total viral transcripts was found in a culture condition in which NW—1 cells grow relatively well and induction of HPV—16 total transcripts occurres 20hr after treating the cells with 5ng/ml Epidermal Growth Factor and 10 μg/ml Insulin with or without 15 μg/ml bovine pituitary extract. Additionally, RNA polymerase chain reaction (PCR) was used to analyze the transcripts of E6 and E7 genes of HPV-16 in NW—1 cells cultured in MCDB—151 supplemented with different growth additive. Three expected amplification products of 791,608 and 491 base pairs in length were identified in NW—1 cells cultured differently. Contrary to what have been thought, however, DNA synthesis and cell growth of NW—1 showed no direct correlation with quantitative differences of PCR products resulting from different culture conditions. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . 1 前言. . . . . . . . . .5 實驗材料與方法. . . . . . . . . .8 結果. . . . . . . . . .21 討論. . . . . . . . . .27 圖表. . . . . . . . . .33 參考文獻. . . . . . . . . . 48 | |
| dc.language.iso | zh-TW | |
| dc.title | 生長因數對人類乳突狀第十六型病毒DNA轉型之人類鼻腔上皮細胞中病毒基因表現，細胞生長及DNA合成調控之研究 | zh_TW |
| dc.title | The Effects of Growth Factors on Regulation of Viral Gene Expressions, Cell Growth and DNA Synthesis in Normal Human Nasal Epithelial Cells Immortalized by HPV-16 DNA | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 61 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| Appears in Collections: | 動物學研究所 | |
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